We narrowed to 15,358 results for: nts

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pAAV-MBP-2xNLS-tdTomato

Plasmid

#104054

Purpose

An AAV genome encoding expression of the nuclear localized fluorescent protein tdTomato from the MBP promoter

Depositor

Viviana Gradinaru

Article

Challis et al Nat Protoc. 2019 Feb;14(2):379-414. doi:

Insert

2xNLS-tdTomato

Use

AAV

Tags

NLS

Promoter

MBP

Available Since

April 24, 2018

Availability

Academic Institutions and Nonprofits only

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pDGE687

Plasmid

#167299

Purpose

Vector for plant genome editing containing p35S:GFP-Cas9 expression cassette, nptII selection marker, and SlFAST2 and Ca-Bs3 counterselction markers, but not sgRNAs.

Depositor

Johannes Stuttmann

Article

Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

May 21, 2021

Availability

Academic Institutions and Nonprofits only

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pGWB454

Plasmid

#74836

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with mRFP (CaMV35S promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Promoter

CaMV35S

Available Since

Oct. 18, 2016

Availability

Academic Institutions and Nonprofits only

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pENTR E3 pR5A L3-L2

Plasmid

#158732

Purpose

Plant mitoTALEN vector assembly, Entry clone 3 with attL3 L2 (last HD)

Depositor

Shin-ichi Arimura

Article

Kazama et al Nat Plants. 2019 Jul;5(7):722-730. doi:

Insert

TALEN ORF without TAL repeat

Use

TALEN

Available Since

Sept. 17, 2020

Availability

Academic Institutions and Nonprofits only

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Phi3-GCaMP6f

Plasmid

#106298

Purpose

CMV driven GFP based calcium (Ca2+) reporter

Depositor

Sanford Simon

Article

Belote et al J Cell Biol. 2020 Jan 6;219(1). pii: 132

Insert

GCaMP6f

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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pENTR E1 pF7A L1-L4

Plasmid

#158730

Purpose

Plant mitoTALEN vector assembly, Entry clone 1 with attR4 R3 (last NI)

Depositor

Shin-ichi Arimura

Article

Kazama et al Nat Plants. 2019 Jul;5(7):722-730. doi:

Insert

TALEN ORF without TAL repeat

Use

TALEN

Available Since

Sept. 17, 2020

Availability

Academic Institutions and Nonprofits only

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p2T-CMV-eA3A-BE5-NG-BlastR

Plasmid

#152998

Purpose

High purity C-to-T base editor

Depositor

David Liu

Article

Arbab et al Cell. 2020 Jun 9. pii: S0092-8674(20)306

Insert

eA3A-BE4 T44D+S45A-nSpCas9-UGI-UGI

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

July 24, 2020

Availability

Academic Institutions and Nonprofits only

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pGWB544

Plasmid

#74878

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with eCFP (CaMV35S promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Promoter

CaMV35S

Available Since

July 20, 2016

Availability

Academic Institutions and Nonprofits only

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pGWB502

Plasmid

#74844

Purpose

Gateway cloning compatible binary vector for expression of gene by CaMV35S promoter.

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Promoter

CaMV35S

Available Since

Aug. 3, 2016

Availability

Academic Institutions and Nonprofits only

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CHMP6_GFP

Plasmid

#31806

Depositor

Daniel Gerlich

Article

Guizetti et al Science. 2011 Mar 25;331(6024):1616-20.

Insert

CHMP6 (CHMP6 Human)

Expression

Mammalian

Available Since

Oct. 5, 2011

Availability

Academic Institutions and Nonprofits only

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pAGM55273

Plasmid

#153211

Purpose

Level 2 cloning vector for a single guide RNA, already contains Cas9, Kanamycin selection cassette for plant transformation and FAST marker

Depositor

Sylvestre Marillonnet

Article

Grutzner et al Plant Commun. 2020 Nov 23;2(2):100135. d

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pComb3XTT

Plasmid

#63891

Purpose

Backbone of pComb3X containing TT Fab (light and heavy chains) against tetanus toxin, used as phage display/expression control and PCR template for human antibody constant regions (kappa and CH1)

Depositor

Carlos Barbas

Article

Andris-Widhopf et al J Immunol Methods. 2000 Aug 28;242(1-2):

Insert

TT Fab

Use

Phage display

Tags

6xHis, HA tag, and geneIII

Expression

Bacterial

Promoter

lacZ

Available Since

July 21, 2015

Availability

Academic Institutions and Nonprofits only

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pCDNA-3xHA-Reptin

Plasmid

#51636

Purpose

Mamamlian expression of 3xHA tagged reptin, N-terminal.

Depositor

Steven Artandi

Article

Venteicher et al Cell. 2008 Mar 21;132(6):945-57. doi: 10

Insert

reptin (RUVBL2 Human)

Tags

3xHA

Expression

Mammalian

Promoter

CMV

Available Since

May 19, 2014

Availability

Academic Institutions and Nonprofits only

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pET28C-mCherry-YTHDF2

Plasmid

#204406

Purpose

E. coli expression of full-length YTHDF2 with an N-terminal mCherry tag

Depositor

Samie Jaffrey

Article

Dumelie et al Nat Chem Biol. 2023 Nov 16. doi: 10.1038

Insert

YTHDF2 (YTHDF2 Human)

Tags

6x-His; mCherry

Expression

Bacterial

Promoter

T7

Available Since

Sept. 5, 2023

Availability

Academic Institutions and Nonprofits only

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GFP-noPS

Plasmid

#177943

Purpose

Transient mammalian expression of eGFP. Lacks packaging sequence and is used as a negative control transfer plasmid for SARS-CoV-2 virus-like particles.

Depositor

Jennifer Doudna

Article

Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

Insert

eGFP

Expression

Mammalian

Available Since

Dec. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 3xFLAG TPP1 88-544

Plasmid

#53548

Purpose

mammalian expression of TPP1 full length

Depositor

Kathleen Collins

Article

Sexton et al J Biol Chem. 2012 Oct 5;287(41):34455-64

Insert

TPP1 (ACD Human)

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

May 29, 2014

Availability

Academic Institutions and Nonprofits only
pAAV-CAG-DIO-EYFP

Plasmid

#104052

Purpose

An AAV genome encoding Cre-dependent expression of the fluorescent protein EYFP from the CAG promoter

Depositor

Viviana Gradinaru

Article

Challis et al Nat Protoc. 2019 Feb;14(2):379-414. doi:

Has Service

AAV PHP.V1

Insert

EYFP

Use

AAV

Promoter

CAG

Available Since

Jan. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-SARS-CoV-2-S (Wuhan)

Plasmid

#215812

Purpose

Expresses SARS-CoV-2 Spike protein in mammalian cells

Depositor

Thomas Gallagher

Article

Qing et al mBio. 2021 Aug 31;12(4):e0159021. doi: 1

Insert

SARS-CoV-2 Spike (S )

Expression

Mammalian

Available Since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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pBABE-hygro CDK4 R24C

Plasmid

#11254

Depositor

Bob Weinberg

Article

Hahn et al Mol Cell Biol 2002 Apr;22(7):2111-23.

Insert

CDK4 (CDK4 Human)

Use

Retroviral

Expression

Mammalian

Mutation

R24C

Available Since

Feb. 14, 2006

Availability

Academic Institutions and Nonprofits only

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pQE-WT-RGVG-M6

Plasmid

#70172

Purpose

Expresses RGVG-M6 T7 RNA polymerase variant with enhanced transcription of 2' modified RNA

Depositor

Andrew Ellington

Article

Meyer et al Nucleic Acids Res. 2015 Sep 3;43(15):748

Insert

RGVG-M6 T7 RNA polymerase

Tags

His

Expression

Bacterial

Promoter

T5/Lac

Available Since

Dec. 16, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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