We narrowed to 19,562 results for: Tec

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3xHA-TurboID_pAS31

Plasmid

#118220

Purpose

expresses 3xHA-tagged TurboID in C. elegans intestine

Depositor

Jessica Feldman

Article

Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

Insert

TurboID (BirA mutant)

Tags

3x HA tag

Expression

Worm

Mutation

Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…

Promoter

ges-1p

Available Since

Jan. 14, 2019

Availability

Academic Institutions and Nonprofits only

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FLHKI-pGFPN3

Plasmid

#21917

Depositor

Hossein Ardehali

Article

Sun et al Mol Cell Biol. 2008 Feb . 28(3):1007-17.

Insert

Full length human HKI in pGFP-N3 (HK1 Human)

Tags

GFP

Expression

Mammalian

Mutation

This is full length human HKI in pGFP-N3

Available Since

Sept. 17, 2009

Availability

Academic Institutions and Nonprofits only

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pGWB402

Plasmid

#74796

Purpose

Gateway cloning compatible binary vector for expression of gene by CaMV35S promoter.

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Promoter

CaMV35S

Available Since

July 22, 2016

Availability

Academic Institutions and Nonprofits only

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pBiFC-bJunVN173

Plasmid

#22012

Depositor

Chang-Deng Hu

Article

Shyu et al Biotechniques. 2006 Jan . 40(1):61-6.

Insert

bJunVN173 (Jun Rat, Aequorea Victoria)

Tags

FLAG

Expression

Mammalian

Mutation

c-Jun(257-338)-Venus(1-172)

Available Since

Sept. 28, 2009

Availability

Academic Institutions and Nonprofits only

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pTLR repair vector

Plasmid

#64322

Purpose

Vector for repair of the traffic light reporter (TLR), providing the venus coding region

Depositor

Ralf Kuehn

Article

Chu et al Nat Biotechnol. 2015 Mar 24. doi: 10.103

Insert

Venus

Use

Artificial targeting vector

Mutation

see publication for details

Promoter

none

Available Since

May 19, 2015

Availability

Academic Institutions and Nonprofits only

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BPK3079

Plasmid

#78741

Purpose

Human expression plasmid for AsCpf1 guide RNA (need to clone in spacer into BsmBI sites)

Depositor

Keith Joung

Article

Kleinstiver et al Nat Biotechnol. 2016 Jun 27. doi: 10.103

Insert

AsCpf1 crRNA backbone, without spacer sequence

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

June 16, 2016

Availability

Academic Institutions and Nonprofits only

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RNA motif plasmid cloning backbone

Plasmid

#107253

Purpose

RNA motif plasmid with BoxB sites. Digest with BsmBI and insert RNA motif of interest. Stuffer size 1.8kb

Depositor

Paul Khavari

Article

Ramanathan et al Nat Methods. 2018 Feb 5. pii: nmeth.4601

Insert

RNA motif plasmid cloning backbone

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

March 15, 2018

Availability

Academic Institutions and Nonprofits only

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ND4.2-Left TALE-G1397-N-dead-mCherry

Plasmid

#179683

Purpose

Expression of base editor in mammalian cells and for cell sorting

Depositor

David Liu

Article

Mok et al Nat Biotechnol. 2022 Sep;40(9):1378-1387

Insert

COX8a MTS_3xFLAG_ND4.2 Left TALE_2aa_DddA G1397-N (E1347A)_1xUGI_P2A_mCherry_ATP5B 3'UTR

Expression

Mammalian

Mutation

E1347A

Available Since

April 4, 2022

Availability

Academic Institutions and Nonprofits only

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pXPR_123

Plasmid

#96919

Purpose

for CRISPRi, lentiviral expression of dCas9-p300 2A BlastR.

Depositor

John Doench, David Root

Article

Najm et al Nat Biotechnol. 2018 Feb;36(2):179-189.

Insert

dCas9

Use

Lentiviral

Tags

p300

Expression

Mammalian

Mutation

dCas9

Promoter

EF1a

Available Since

Nov. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pGWB411

Plasmid

#74805

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with FLAG (CaMV35S promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Promoter

CaMV35S

Available Since

Oct. 18, 2016

Availability

Academic Institutions and Nonprofits only

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pDonor_attP-Ef1a-Puro-P2A-mCherry

Plasmid

#247159

Purpose

attP donor plasmid expressing puromycin resistance and mCherry

Depositor

Patrick Hsu

Article

Fanton et al Nat Biotechnol. 2025 Nov 6. doi: 10.1038

Insert

Puromycin resistance, mCherry

Expression

Mammalian

Promoter

Ef1a

Available Since

Nov. 12, 2025

Availability

Academic Institutions and Nonprofits only

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v1em-Nterm-PE2max-U6-sgRNA

Plasmid

#198732

Purpose

AAV genome encoding N-terminal PE2max and U6 expression cassette

Depositor

David Liu

Article

Davis et al Nat Biotechnol. 2023 May 4. doi: 10.1038

Insert

Nterm PE2max-NpuN

Use

AAV

Promoter

EFS

Available Since

May 4, 2023

Availability

Academic Institutions and Nonprofits only

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pCfB2312 (TEF1p-Cas9-CYC1t_kanMX)

Plasmid

#78231

Purpose

Expresses Cas9 protein, under the control of the TEF1 promoter, and CYC terminator. KanMX resistance

Depositor

Irina Borodina

Article

Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

Insert

Cas9

Expression

Yeast

Available Since

Nov. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pHS_Nova

Plasmid

#239831

Purpose

NovaIscB mammalian expression

Depositor

Feng Zhang

Article

Kannan et al Nat Biotechnol. 2025 May 7. doi: 10.1038

Insert

NovaIscB

Tags

Nucleoplasmin NLS-3xHA and SV40 NLS

Expression

Mammalian

Mutation

Insertion of REC domain from NbaCas9-1 and swap o…

Promoter

CMV

Available Since

July 21, 2025

Availability

Academic Institutions and Nonprofits only

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pGTMV

Plasmid

#118755

Purpose

Binary plasmid with a tobacco mosaic virus infectious clone; Agrobacterium-mediated transformation required for expression in plants.

Depositor

Jose-Antonio Daros

Article

Shi et al Plant Biotechnol J. 2018 Nov 22. doi: 10

Insert

Tobacco mosaic virus

Expression

Plant

Promoter

Cauliflower mosaic virus 35S

Available Since

Nov. 30, 2018

Availability

Academic Institutions and Nonprofits only

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pAF0689 Ef1a-Pa01_attB-BFP acceptor

Plasmid

#193472

Purpose

Acceptor plasmid for 3 plasmid recombination assay

Depositor

Patrick Hsu

Article

Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

Insert

Pa01_attB

Expression

Mammalian

Promoter

Ef1a

Available Since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pQC.Flip-nluc(LgBiT1-8)CP[Casp3A]-Fluc

Plasmid

#200123

Purpose

Retroviral expression of Flip-nluc(LgBiT1-8)CP[Casp3A] protease reporter and P2A firefly luciferase in mammalian cells

Depositor

Eiji Morita

Article

Arakawa et al Sci Rep. 2023 Jan 31;13(1):1753. doi: 10

Insert

Flip-nluc(LgBiT1-8)CP[Casp3A] P2A firefly luciferase

Use

Retroviral

Expression

Mammalian

Promoter

CMV

Available Since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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Factor IX donor

Plasmid

#182141

Purpose

Factor IX donor for twinPE and Bxb1 mediated insertion at ALB

Depositor

David Liu

Article

Anzalone et al Nat Biotechnol. 2022 May;40(5):731-740.

Insert

attP-Factor IX intron 1 and exon 2-8 CDS and exon 8 3'UTR

Expression

Mammalian

Available Since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

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pUAS-V5-miniTurboID-NES

Plasmid

#116905

Purpose

Expresses miniTurboID under Gal4/UAS control. miniTurboID is tagged at the N-terminus with V5 and a nuclear export signal (NES) at the C-terminus.

Depositor

Norbert Perrimon

Article

Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

Insert

miniTurboID

Use

Drosophila expression

Available Since

Oct. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pGWB417

Plasmid

#74811

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with 4xMyc (CaMV35S promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Promoter

CaMV35S

Available Since

July 14, 2016

Availability

Academic Institutions and Nonprofits only

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