We narrowed to 19,051 results for: Tec;

1. pMT450

   Plasmid

   #139390

   Purpose

   Bile Acid inducible sgRNA-3 with PM3 driving Nanoluc expression (NOT Gate 3), pNBU1 backbone, AmpR, ErmR

   Depositor

   Christopher Voigt

   Article

   Taketani et al Nat Biotechnol. 2020 Aug;38(8):962-969.

   Insert

   Bile Acid inducible sgRNA-3 with PM3 driving Nanoluc expression (NOT Gate 3), pNBU1 backbone, AmpR, ErmR
   

   Use

   Synthetic Biology

   Promoter

   Bile Acid inducible Promoter

   Available Since

   Jan. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMT492

   Plasmid

   #139397

   Purpose

   Bile Acid inducible sgRNA-5 with PM5 driving Nanoluc expression (NOT Gate 5), pNBU1 backbone, AmpR, ErmR

   Depositor

   Christopher Voigt

   Article

   Taketani et al Nat Biotechnol. 2020 Aug;38(8):962-969.

   Insert

   Bile Acid inducible sgRNA-5 with PM5 driving Nanoluc expression (NOT Gate 5)
   

   Use

   Synthetic Biology

   Promoter

   Bile Acid inducible Promoter

   Available Since

   Jan. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. Actin Binding Domain Tensionless Control

   Plasmid

   #215371

   Purpose

   Expresses Actin Binding Domain Tensionless Control (ABDTL), composed of Ftractin-Linker-TSMod. This is the unloaded control for Actin Binding Domain Tension Sensor (ABDTS).

   Depositor

   Brenton Hoffman

   Article

   Shoyer et al Cell Rep Methods. 2024 Jul 15;4(7):10081

   Insert

   Actin Binding Domain Tensionless Control
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDD315 (mTurquoise2^SEC^2xHA)

   Plasmid

   #73343

   Purpose

   mTurquoise2^SEC^2xHA vector with ccdB sites for cloning homology arms

   Depositor

   Bob Goldstein

   Article

   Zhang et al J Cell Biol. 2023 Sep 4;222(9):e20230210

   Insert

   mTurquoise2-C1^SEC^3xFlag
   

   Use

   CRISPR and Cre/Lox

   Tags

   2xHA and C. elegans codon-optimized mTurquoise2

   Expression

   Worm

   Available Since

   March 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pUC57 APEX

   Plasmid

   #40306

   Purpose

   A monomeric and improved-activity variant of pea ascorbate peroxidase. Used for electron microscopy and live-cell proteomic mapping. This construct has been replaced by APEX2 (construct 49386).

   Depositor

   Alice Ting

   Article

   Martell et al Nat Biotechnol. 2012 Oct 21. doi: 10.103

   Insert

   pea APEX (Synthetic)
   

   Use

   Unspecified

   Mutation

   K14D, W41F, E112K (relative to wild-type)

   Available Since

   Sept. 5, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCellFree_G03 CIRBP

   Plasmid

   #67055

   Purpose

   Standard for cell-free expression benchmarking.

   Depositor

   Kirill Alexandrov

   Article

   Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

   Insert

   CIRBP (CIRBP Human)
   

   Use

   Cell-free expression

   Tags

   EGFP and His

   Promoter

   T7

   Available Since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

7. pBAD-mNectarine

   Plasmid

   #21717

   Purpose

   Bacterial expression of pH-sensitive monomeric mNectarine red fluorescent protein

   Depositor

   Robert Campbell

   Article

   Johnson et al J Biol Chem. 2009 Jul 31;284(31):20499-5

   Insert

   mNectarine fluorescent proteins
   

   Tags

   His

   Expression

   Bacterial

   Mutation

   The fluorescent protein was inserted between XhoI…

   Available Since

   Aug. 11, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTS2311-Tier1-spacer-PPGK-NLuc_TB

   Plasmid

   #169552

   Purpose

   Tier-1 expression vector encoding PmPGK1-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 preluded by a 5' spacer sequence (spacer-PmPGK1-IgkSS-Nluc-p2A-mTagBFP2-pA).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   PPGK-NanoLuc Luciferase - mTagBFP fusion
   

   Expression

   Mammalian

   Promoter

   PPGK

   Available Since

   June 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pYPQ166

   Plasmid

   #109328

   Purpose

   Gateway entry plasmid (attL1 & attR5) expressing 3_FLAG-NLS-zCas9-NLS without promoter

   Depositor

   Yiping Qi

   Article

   Lee et al Plant Biotechnol J. 2018 Jul 4. doi: 10.

   Insert

   zCas9
   

   Use

   CRISPR; Gateway compatible zcas9 entry clone

   Tags

   3x FLAG, NLS and NLS

   Expression

   Plant

   Mutation

   Zea mays codon-optimized Cas9

   Available Since

   June 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRDB_118

    Plasmid

    #216089

    Purpose

    Cas9 [Sp] CRISPRa targeting CD47, positive control

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Insert

    CD47 guide
    

    Use

    CRISPR and Lentiviral; Positive controls

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pRT498-3xOtUBD

    Plasmid

    #190088

    Purpose

    Expresses 3 tandem OtUBD with an TEV-cleavable N-terminal 6xHis-MBP tag as well as an engineered N-terminal cysteine in E. coli

    Depositor

    Mark Hochstrasser

    Article

    Zhang et al PLoS Biol. 2022 Jun 30;20(6):e3001501. d

    Insert

    3xOtUBD
    

    Tags

    6xHis and MBP

    Expression

    Bacterial

    Available Since

    Oct. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGEL031

    Plasmid

    #137900

    Purpose

    STU2.0 SpyCas9 tRNA system for plant genome editing

    Depositor

    Yong Zhang

    Article

    Tang et al Plant Biotechnol J. 2019 Jul;17(7):1431-

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Plant

    Available Since

    Feb. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3.1(+)-blueAKAR(T/A)

    Plasmid

    #118471

    Purpose

    Negative-control mutant for blueAKAR biosensor.

    Depositor

    Jin Zhang

    Article

    Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

    Insert

    blueAKAR(T/A)
    

    Tags

    6xHis, T7 tag (gene 10 leader), and Xpress (TM) t…

    Expression

    Mammalian

    Mutation

    Contains Thr-to-Ala mutation in substrate sequenc…

    Promoter

    CMV

    Available Since

    April 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMT493

    Plasmid

    #139398

    Purpose

    Bile Acid inducible sgRNA-6 with PM6 driving Nanoluc expression (NOT Gate 6), pNBU1 backbone, AmpR, ErmR

    Depositor

    Christopher Voigt

    Article

    Taketani et al Nat Biotechnol. 2020 Aug;38(8):962-969.

    Insert

    Bile Acid inducible sgRNA-6 with PM6 driving Nanoluc expression (NOT Gate 6)
    

    Use

    Synthetic Biology

    Promoter

    Bile Acid inducible Promoter

    Available Since

    April 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pMT494

    Plasmid

    #139399

    Purpose

    Bile Acid inducible sgRNA-7 with PM7 driving Nanoluc expression (NOT Gate 7), pNBU1 backbone, AmpR, ErmR

    Depositor

    Christopher Voigt

    Article

    Taketani et al Nat Biotechnol. 2020 Aug;38(8):962-969.

    Insert

    Bile Acid inducible sgRNA-7 with PM7 driving Nanoluc expression (NOT Gate 7)
    

    Use

    Synthetic Biology

    Promoter

    Bile Acid inducible Promoter

    Available Since

    April 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pMT449

    Plasmid

    #139389

    Purpose

    Bile Acid inducible sgRNA-2 with PM2 driving Nanoluc expression (NOT Gate 2), pNBU1 backbone, AmpR, ErmR

    Depositor

    Christopher Voigt

    Article

    Taketani et al Nat Biotechnol. 2020 Aug;38(8):962-969.

    Insert

    Bile Acid inducible sgRNA-2 with PM2 driving Nanoluc expression (NOT Gate 2)
    

    Use

    Synthetic Biology

    Promoter

    Bile Acid inducible Promoter

    Available Since

    April 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMT455

    Plasmid

    #139392

    Purpose

    aTC and Bile Acid inducible sgRNA-4 with PM4 driving Nanoluc expression (NOR Gate), AmpR, ErmR

    Depositor

    Christopher Voigt

    Article

    Taketani et al Nat Biotechnol. 2020 Aug;38(8):962-969.

    Insert

    aTC and Bile Acid inducible sgRNA-4 with PM4 driving Nanoluc expression (NOR Gate)
    

    Use

    Synthetic Biology

    Promoter

    aTC and BA inducible promoters

    Available Since

    April 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. attB-puro-GA donor

    Plasmid

    #182138

    Purpose

    attB-puro-GA DNA donor for twinPE and Bxb1 mediated gene-size insertion

    Depositor

    David Liu

    Article

    Anzalone et al Nat Biotechnol. 2022 May;40(5):731-740.

    Insert

    attB-GA-EGFP-EF1α-PuroR-T2A-TagBFP
    

    Expression

    Mammalian

    Available Since

    March 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. NG-ABE8-dimer

    Plasmid

    #138497

    Purpose

    A-to-G base editor

    Depositor

    David Liu

    Article

    Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

    Insert

    ecTadA(WT)-ecTadA(8e)-nSpCas9 (NG)
    

    Expression

    Mammalian

    Available Since

    March 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. Sec61b-HA-mNG21-10

    Plasmid

    #141213

    Purpose

    Membrane Tethered Split Fluorescent Reporter for ER Retragrade

    Depositor

    Wayne Lencer

    Article

    Luong et al Mol Biol Cell. 2019 Nov 27:mbcE19070375.

    Insert

    Sec61b-HA-mNG21-10
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Available Since

    July 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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