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PERK 4: PERKKD-pGEX4T-1

Plasmid

#21817

Depositor

David Ron

Article

Harding et al Nature. 1999 Jan 21. 397(6716):271-4.

Insert

PERK (537-1114) (Eif2ak3 Mouse)

Tags

GST and Myc (9E10)

Expression

Bacterial

Mutation

PERK Kinase Domain. Amino acids 537-1114.

Available Since

March 29, 2010

Availability

Academic Institutions and Nonprofits only

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pHAGE-UBC-TagRFP-T-ps

Plasmid

#178656

Purpose

Expression of palmitoylated TagRFP-T for live imaging of plasma membrane (protrusions, tunneing nanotubes), in mammalian cells

Depositor

Jeffrey Gerst

Article

Haimovich et al Proc Natl Acad Sci U S A. 2017 Nov 14;11

Insert

TagRFP-T with palmitoylation signal at N-terminus

Use

Lentiviral

Tags

Palmitoylation signal

Promoter

Ubc

Available Since

May 10, 2022

Availability

Academic Institutions and Nonprofits only

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pEG302 22aa SunTag VP64 g4+g17

Plasmid

#120252

Purpose

CRISPR-Cas9 SunTag system to target VP64 to the FWA locus with two guide RNAs

Depositor

Steven Jacobsen

Article

Papikian et al Nat Commun. 2019 Feb 13;10(1):729. doi:

Insert

g17_U6_g4_U6_NOS_NLS_GB1_NLS_linker_VP64_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_3xNLS_linker_10xGCN4_OCS

Use

CRISPR and Synthetic Biology

Expression

Plant

Available Since

March 17, 2019

Availability

Academic Institutions and Nonprofits only

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pEG302 22aa SunTag NtDRMcd g4+g10+g18 (FWA)

Plasmid

#115487

Purpose

CRISPR Cas9 SunTag system to target NtDRMcd to the FWA locus with three guide RNAs

Depositor

Steven Jacobsen

Article

Papikian et al Nat Commun. 2019 Feb 13;10(1):729. doi:

Insert

g18_U6_g10_U6_g4_U6_NOS_NLS_GB1_NLS_linker_DRMcd_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_3xNLS_linker_10xGCN4_OCS

Use

CRISPR and Synthetic Biology

Expression

Plant

Available Since

April 2, 2019

Availability

Academic Institutions and Nonprofits only

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pMIEG3-BCL6

Plasmid

#40339

Depositor

Alexander Dent

Article

Toney et al Nat Immunol. 2000 Sep;1(3):214-20.

Insert

BCL6 (BCL6 Human)

Use

Retroviral

Tags

IRES eGFP

Expression

Mammalian

Mutation

optimized translation initiation codon

Available Since

Sept. 28, 2012

Availability

Academic Institutions and Nonprofits only
LRP6N-IgG-pCS2

Plasmid

#27279

Depositor

Xi He

Article

Tamai et al Nature. 2000 Sep 28. 407(6803):530-5.

Insert

low density lipoprotein receptor-related protein 6 (LRP6 Human)

Use

Xenopus injection

Tags

IgG Fc

Available Since

Feb. 10, 2011

Availability

Academic Institutions and Nonprofits only
Luc-SIRT1 mutant 3'UTR

Plasmid

#20380

Depositor

Charles Lowenstein

Article

Yamakuchi et al Proc Natl Acad Sci U S A. 2008 Sep 9. 10

Insert

SIRT1 (SIRT1 Human)

Use

Luciferase

Mutation

mutant 3'UTR of SIRT1 (ACACCCACCAAGGACCATTAG…

Available Since

May 1, 2009

Availability

Academic Institutions and Nonprofits only

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pEG302 5aa SunTag VP64 nog

Plasmid

#115480

Purpose

CRISPR-Cas9 SunTag system to target VP64 to specific loci of interest (nog=no guide)

Depositor

Steven Jacobsen

Article

Papikian et al Nat Commun. 2019 Feb 13;10(1):729. doi:

Insert

NOS_NLS_GB1_NLS_linker_VP64_linker sfGFP_scFv_UBQ10_Insulator UBQ10_Ω dCas9_1xHA_2xNLS_linker_10xGCN4_OCS

Use

CRISPR and Synthetic Biology

Expression

Plant

Available Since

March 17, 2019

Availability

Academic Institutions and Nonprofits only

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PXPR007 sgGFP-1

Plasmid

#74960

Purpose

Cas9 + sgGFP-1 with blasticidin selection

Depositor

William Hahn

Article

Wang et al Cell Rep. 2017 Feb 7;18(6):1543-1557. do

Insert

GFP

Use

CRISPR

Expression

Mammalian

Available Since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

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pTn7·X-nia

Plasmid

#122592

Purpose

Plasmid for genomic integraction of xylS-Pm->nia into the Tn7 insertion site

Depositor

Pablo Ivan Nikel

Article

Volke et al Microb Biotechnol. 2019 Mar 12. doi: 10.

Insert

xylS/Pm->Nia

Use

Synthetic Biology; Tn7 genomic integration

Expression

Bacterial

Promoter

Pm

Available Since

March 22, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA HisB TO rFez1

Plasmid

#17385

Depositor

Stephen Goff

Article

Naghavi et al Genes Dev. 2005 May 1. 19(9):1105-15.

Insert

FEZ1 (Fez1 Rat)

Tags

his and myc

Expression

Mammalian

Available Since

March 1, 2010

Availability

Academic Institutions and Nonprofits only
pAAV_tKiMBI-T2A-caMEK

Plasmid

#199578

Purpose

Expresses tKiMBI and caMEK in an AAV vector

Depositor

Michael Lin

Article

Wu et al ACS Cent Sci. 2023 Mar 20;9(4):719-732.

Inserts

ERK tdTomato-Kinase-Modulated Bioluminescent Indicator
constitutively active MEK

Use

AAV

Expression

Mammalian

Promoter

CMV

Available Since

June 23, 2023

Availability

Academic Institutions and Nonprofits only

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pFAB808

Plasmid

#47853

Purpose

BIOFAB reporter plasmid for measuring rrnB T1 termination efficiency

Depositor

Drew Endy

Article

Mutalik et al Nat Methods. 2013 Apr;10(4):354-60. doi:

Insert

rrnB T1 terminator

Use

Synthetic Biology

Expression

Bacterial

Promoter

pLTetO8

Available Since

Dec. 3, 2013

Availability

Academic Institutions and Nonprofits only

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LZF1826 pAAV_hSyn-DiO-SomArchon-EGFP-P2A-stGtACR2

Plasmid

#135412

Purpose

simultaneous optical recording and manipulation of electrical activity in neurons

Depositor

Adam Cohen

Article

Fan et al Cell. 2020 Jan 23. pii: S0092-8674(20)30

Insert

SomArchon-EGFP-P2A-stGtACR2

Use

AAV

Expression

Mammalian

Available Since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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GST-MAGE-2-429

Plasmid

#89129

Purpose

bacterial expression of full-length human MAGE-A11 with N-terminal GST tag for rapid isolation, in pGEX-4T-1 EcoRI and XhoI sites

Depositor

Elizabeth Wilson

Article

Askew et al Mol Cell Endocrinol. 2017 Mar 5;443:42-5

Insert

MAGE-A11 (MAGEA11 Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

April 10, 2017

Availability

Academic Institutions and Nonprofits only
RIPR-bio-His

Plasmid

#50824

Purpose

Expresses enzymatically monobiotinylated full length RIPR

Depositor

Gavin Wright

Article

Zenonos et al Malar J. 2014 Mar 12;13(1):93. doi: 10.1

Insert

RIPR

Tags

ratCD4d3+4,biotinylation sequence, 6xHis

Expression

Mammalian

Mutation

all potential N-linked glycosylation sites (N-X-S…

Promoter

CMV

Available Since

Feb. 11, 2014

Availability

Academic Institutions and Nonprofits only

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pHL 1075

Plasmid

#53004

Purpose

pCon:fhlA::mCherry, pLlacO-1:sodB::gfp

Depositor

Han Lim

Article

Hussein et al Proc Natl Acad Sci U S A. 2011 Jan 18;10

Inserts

fhlA
sodB

Use

Synthetic Biology

Tags

GFP and mCherry

Expression

Bacterial

Mutation

contains −309 to +96 bp relative to start codon a…

Promoter

pCon and pLlacO-1

Available Since

May 16, 2014

Availability

Academic Institutions and Nonprofits only
pARF1-YFP

Plasmid

#11390

Depositor

Joel Swanson

Article

Beemiller et al PLoS Biol. 2006 May 9. 4(6):e162.

Insert

ADP Ribosylation Factor 1 (ARF1 Human)

Tags

YFP

Expression

Mammalian

Available Since

May 30, 2006

Availability

Academic Institutions and Nonprofits only

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pFAB815

Plasmid

#47854

Purpose

BIOFAB reporter plasmid for measuring rnpB T1 termination efficiency

Depositor

Drew Endy

Article

Mutalik et al Nat Methods. 2013 Apr;10(4):354-60. doi:

Insert

rnpB T1 terminator

Use

Synthetic Biology

Expression

Bacterial

Promoter

pLTetO9

Available Since

Dec. 3, 2013

Availability

Academic Institutions and Nonprofits only

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pJZC519

Plasmid

#62278

Purpose

Yeast dCas9-VP64 expression plasmid

Depositor

Wendell Lim, Stanley Qi

Article

Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

Insert

dCas9-VP64

Tags

Has a VP64 fusion

Expression

Yeast

Mutation

Has mutations in the RuvC1 and HNH domains to ren…

Promoter

pTdh3

Available Since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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