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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 36,022 results for: CaS;

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Showing: 5961 - 5980 of 36022 results

  5. pcDNA3_erGAP3

    Plasmid
    #78118
    Purpose
    Low affinity fluorescent calcium indicator of the GAP (GFP-Aequorin Protein) family targeted to the endoplasmic reticulum
    Depositor
    Teresa Alonso, Javier García-Sancho
    Article
    Navas-Navarro et al Cell Chem Biol. 2016 Jun 1. pii: S2451-9
    Insert
    erGAP3
    Tags
    KDEL and calreticulin signal peptide
    Expression
    Mammalian
    Mutation
    S175G, D180Y, I167V and L15Q in GFP moiety and D…
    Promoter
    CMV
    Available Since
    July 21, 2016
    Availability
    Academic Institutions and Nonprofits only
    78118-plasmid-map-sequence-id-153361

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  6. pA-CBh.TP-CNOT8-V5H6.MCh.Puro

    Plasmid
    #209947
    Purpose
    In mammalian cells expresses TP fused to a CNOT8 with a V5H6 tag. Also expresses an mCherry fluorescent protein and puromycin resistance marker.
    Depositor
    Kevin Weeks
    Article
    Hatfield et al Biochemistry. 2025 Sep 16;64(18):3854-38
    Inserts
    CCR4-NOT transcription complex subunit 8 (CNOT8 Human)
    mCherry fluorescent protein fused to puromycin resistance marker
    Tags
    TP (MS2 coat protein) and V5 epitope with H6 tag
    Expression
    Mammalian
    Promoter
    CBh and CMV
    Available Since
    July 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    209947-plasmid-map-sequence-id-418725

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  7. pNon-target

    Plasmid
    #214053
    Purpose
    Expresses non-target sequence for Haliangium type III CRISPR-Cas complex; identical to pTarget (Addgene plasmid # 214052) but does not contain a protospacer.
    Depositor
    Raymond Staals
    Article
    Steens et al Science. 2024 Feb 2;383(6682):512-519. d
    Insert
    Non-target sequence
    Use
    Non-target rna plasmid
    Expression
    Bacterial
    Promoter
    trc promoter
    Available Since
    June 14, 2024
    Availability
    Academic Institutions and Nonprofits only
    214053-plasmid-map-sequence-id-428839

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  8. pTarget

    Plasmid
    #214052
    Purpose
    Expresses target sequence for Haliangium type III CRISPR-Cas complex; identical to pNon-target (Addgene plasmid # 214053) but contains a protospacer.
    Depositor
    Raymond Staals
    Article
    Steens et al Science. 2024 Feb 2;383(6682):512-519. d
    Insert
    Target sequence
    Use
    Target rna plasmid
    Expression
    Bacterial
    Promoter
    trc promoter
    Available Since
    June 14, 2024
    Availability
    Academic Institutions and Nonprofits only
    214052-plasmid-map-sequence-id-428822

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  10. pEcNucC

    Plasmid
    #214050
    Purpose
    Bacterial expression plasmid for E. coli MS 115-1 NucC, a cA3-responsive nuclease known to cause abortive infection; used as a positive control in the Haliangium ochraceum type III CRISPR-Cas system
    Depositor
    Raymond Staals
    Article
    Steens et al Science. 2024 Feb 2;383(6682):512-519. d
    Insert
    EcNucC
    Expression
    Bacterial
    Mutation
    WT
    Promoter
    lacUV5 promoter
    Available Since
    June 14, 2024
    Availability
    Academic Institutions and Nonprofits only
    214050-plasmid-map-sequence-id-428997

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  11. pA-CBh.TP-CNOT7-V5H6.MCh.Puro

    Plasmid
    #209946
    Purpose
    In mammalian cells expresses TP fused to a CNOT7 with a V5H6 tag. Also expresses an mCherry fluorescent protein and puromycin resistance marker.
    Depositor
    Kevin Weeks
    Article
    Hatfield et al Biochemistry. 2025 Sep 16;64(18):3854-38
    Inserts
    CCR4-NOT transcription complex subunit 7 (CNOT7 Human)
    mCherry fluorescent protein fused to puromycin resistance marker
    Tags
    TP (MS2 coat protein) and V5 epitope with H6 tag
    Expression
    Mammalian
    Promoter
    CBh and CMV
    Available Since
    July 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    209946-plasmid-map-sequence-id-418741

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  12. pA-CBh.TP-DCP2-V5H6.MCh.Puro

    Plasmid
    #209948
    Purpose
    In mammalian cells expresses TP fused to a DCP2 with a V5H6 tag. Also expresses an mCherry fluorescent protein and puromycin resistance marker.
    Depositor
    Kevin Weeks
    Article
    Hatfield et al Biochemistry. 2025 Sep 16;64(18):3854-38
    Inserts
    Decapping protein 2 (DCP2 Human)
    mCherry fluorescent protein fused to puromycin resistance marker
    Tags
    TP (MS2 coat protein) and V5 epitope with H6 tag
    Expression
    Mammalian
    Promoter
    CBh and CMV
    Available Since
    July 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    209948-plasmid-map-sequence-id-418740

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  13. pA-CBh.TP-(inactive)CNOT6-V5H6.MCh.Puro

    Plasmid
    #209943
    Purpose
    In mammalian cells expresses TP fused to an inactive CNOT6 with a V5H6 tag. Also expresses an mCherry fluorescent protein and puromycin resistance marker.
    Depositor
    Kevin Weeks
    Article
    Hatfield et al Biochemistry. 2025 Sep 16;64(18):3854-38
    Inserts
    CCR4-NOT transcription complex subunit 6 (enzymatically inactive) (CNOT6 Human)
    mCherry fluorescent protein fused to puromycin resistance marker
    Tags
    TP (MS2 coat protein) and V5 epitope with H6 tag
    Expression
    Mammalian
    Promoter
    CBh and CMV
    Available Since
    July 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    209943-plasmid-map-sequence-id-418717

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  14. pA-CBh.TP-CNOT6-V5H6.MCh.Puro

    Plasmid
    #209944
    Purpose
    In mammalian cells expresses TP fused to a CNOT6 with a V5H6 tag. Also expresses an mCherry fluorescent protein and puromycin resistance marker.
    Depositor
    Kevin Weeks
    Article
    Hatfield et al Biochemistry. 2025 Sep 16;64(18):3854-38
    Inserts
    CCR4-NOT transcription complex subunit 6 (CNOT6 Human)
    mCherry fluorescent protein fused to puromycin resistance marker
    Tags
    TP (MS2 coat protein) and V5 epitope with H6 tag
    Expression
    Mammalian
    Promoter
    CBh and CMV
    Available Since
    July 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    209944-plasmid-map-sequence-id-418715

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  15. pA-CBh.TP-CNOT6L-V5H6.MCh.Puro

    Plasmid
    #209945
    Purpose
    In mammalian cells expresses TP fused to a CNOT6L with a V5H6 tag. Also expresses an mCherry fluorescent protein and puromycin resistance marker.
    Depositor
    Kevin Weeks
    Article
    Hatfield et al Biochemistry. 2025 Sep 16;64(18):3854-38
    Inserts
    CNOT6L (CNOT6L Human)
    mCherry fluorescent protein fused to puromycin resistance marker
    Tags
    TP (MS2 coat protein) and V5 epitope with H6 tag
    Expression
    Mammalian
    Promoter
    CBh and CMV
    Available Since
    July 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    209945-plasmid-map-sequence-id-418727

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  16. 1203A-CMV-5’DR-EGFP-bGHpolyA

    Plasmid
    #221452
    Purpose
    EGFP reporter construct with single unprocessed direct repeat (35nt) at 5' end
    Depositor
    Omar Akbari
    Insert
    eGFP
    Expression
    Mammalian
    Available Since
    Feb. 19, 2025
    Availability
    Academic Institutions and Nonprofits only
    221452-plasmid-map-sequence-id-437434

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  17. 1203B-CMV-3’DR-EGFP-bGHpolyA

    Plasmid
    #221453
    Purpose
    EGFP reporter construct with single unprocessed direct repeat (35nt) at 3' end
    Depositor
    Omar Akbari
    Insert
    eGFP
    Expression
    Mammalian
    Available Since
    Feb. 19, 2025
    Availability
    Academic Institutions and Nonprofits only
    221453-plasmid-map-sequence-id-437437

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Showing: 5961 - 5980 of 36022 results