We narrowed to 12,024 results for: pcdna

1. p5073 pcDNA4c hBrd4 (1047-1223)

   Plasmid

   #14452

   Depositor

   Peter Howley

   Article

   You et al Cell. 2004 Apr 30. 117(3):349-60.

   Insert

   Brd4 (1047-1223) (BRD4 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   amino acids 1047-1223

   Promoter

   Available Since

   March 2, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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2. p5081 pcDNA4c hBrd4 (1134-1223)

   Plasmid

   #14457

   Depositor

   Peter Howley

   Article

   You et al Cell. 2004 Apr 30. 117(3):349-60.

   Insert

   Brd4 (1134-1223) (BRD4 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   amino acids 1134-1223

   Promoter

   Available Since

   March 2, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3 c-SRC (250-536) K298M HA

   Plasmid

   #42210

   Depositor

   Robert Lefkowitz

   Article

   Miller et al J Biol Chem. 2000 Apr 14;275(15):11312-9

   Insert

   c-SRC (250-536) HA (SRC Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   contains amino acids 250-536; K298M

   Promoter

   CMV

   Available Since

   Jan. 28, 2013

   Availability

   Academic Institutions and Nonprofits only

4. pcDNA-dCas9-p300 Core (Y1467F)

   Plasmid

   #61362

   Purpose

   encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664; inavtivating mutation Y1467F) driven by CMV promoter

   Depositor

   Charles Gersbach

   Article

   Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do

   Insert

   S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 Human, Synthetic, S. pyogenes)
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Flag, HA, and SV40 NLS

   Expression

   Mammalian

   Mutation

   D10A; H840A in S.pyogenes Cas9; Y1467F mutation i…

   Promoter

   CMV

   Available Since

   April 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3 mRuby2 LIC cloning vector (6H)

   Plasmid

   #86965

   Purpose

   Empty LIC vector; adds an mRuby2 gene to the C-terminus of your protein of interest.

   Depositor

   Chris Jeans

   Type

   Empty backbone

   Use

   Tags

   mRuby2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   March 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. (137) pcDNA3.1-HA-nEPEA-(EAAAK)4-OGT(4)

   Plasmid

   #160740

   Purpose

   Expresses a truncated OGT (4.5 TPRs) fused to the EPEA nanobody

   Depositor

   Christina Woo

   Article

   Ramirez et al ACS Chem Biol. 2020 Apr 17;15(4):1059-10

   Insert

   HA-nEPEA-(EAAAK)4-OGT(4)
   

   Use

   Tags

   HA-Tag

   Expression

   Mammalian

   Mutation

   Promoter

   T7/CMV

   Available Since

   May 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1-dCas9-MQ1(C141S)-EGFP

   Plasmid

   #89635

   Purpose

   Targeted CpG methylation

   Depositor

   Margaret Goodell

   Article

   Lei et al Nat Commun. 2017 Jul 11;8:16026. doi: 10

   Insert

   site-specific DNA-methyltransferase SssI
   

   Use

   CRISPR

   Tags

   3*FLAG, 6*His, and T2A-EGFP

   Expression

   Mammalian

   Mutation

   C141S, TGC-->TCC

   Promoter

   CMV

   Available Since

   July 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA5/FRT/TO/Flag-tGFP-HuR.wt

   Plasmid

   #110376

   Purpose

   Mammalian expression of turboGFP-HuR (wild type) with FLAG tag, Flp-In T-Rex system

   Depositor

   Sandra Martha Gomes Dias

   Insert

   ELAV like RNA binding protein 1 (ELAVL1 Human)
   

   Use

   Tags

   FLAG and tGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   June 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1 LIC 6A HIS-TEV-DPP8

   Plasmid

   #166793

   Purpose

   Mammalian cell expression vector for Flag-tagged DPP8

   Depositor

   Hao Wu

   Article

   Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

   Insert

   DPP8 (DPP8 Human)
   

   Use

   Tags

   HIS-TEV

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   March 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. Nano-lantern-plasma membrane/pcDNA3

    Plasmid

    #51975

    Purpose

    mammalian expression of Nano-lantern, a luminescent protein for high-speed single-cell and whole body imaging, targeted to the plasma membrane

    Depositor

    Takeharu Nagai

    Article

    Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

    Insert

    Nano-lantern
    

    Use

    Tags

    HIS and Lyn

    Expression

    Mammalian

    Mutation

    S257G in Rluc8

    Promoter

    Available Since

    April 15, 2014

    Availability

    Academic Institutions and Nonprofits only

11. pCDNA3 5'cMyc-SMCl- S957A/S966A

    Plasmid

    #32372

    Depositor

    Michael Kastan

    Article

    Kim et al Genes Dev. 2002 Mar 1;16(5):560-70.

    Insert

    SMC1A (SMC1A Human)
    

    Use

    Tags

    c-Myc

    Expression

    Mammalian

    Mutation

    S957A/S996A

    Promoter

    Available Since

    Sept. 30, 2011

    Availability

    Academic Institutions and Nonprofits only

12. pcDNA3.1-tvD-Cit-tevs-DHFR

    Plasmid

    #116054

    Purpose

    Reporter plasmid for measuring protease activity

    Depositor

    Michael Elowitz

    Article

    Gao et al Science. 2018 Sep 21;361(6408):1252-1258

    Insert

    tvD-Cit-tevs-DHFR
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3-myc3-CUL4A delta N52

    Plasmid

    #19952

    Depositor

    Yue Xiong

    Article

    Liu et al Mol Cell. 2002 Dec . 10(6):1511-8.

    Insert

    CUL4A (cullin 4A) (CUL4A Human)
    

    Use

    Tags

    myc3

    Expression

    Mammalian

    Mutation

    Missing 52 residues from N terminal CUL4A.

    Promoter

    Available Since

    April 1, 2009

    Availability

    Academic Institutions and Nonprofits only

14. pcDNA5 FRT TO Myc ArB WT RNAiR

    Plasmid

    #59806

    Purpose

    Allows the integration of Myc ArB in the genome and Tet-inducible expression.

    Depositor

    Stephen Taylor

    Article

    Girdler et al J Cell Sci. 2006 Sep 1;119(Pt 17):3664-7

    Insert

    Aurora B (AURKB Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    RNAi resistant

    Promoter

    hybrid human cytomegalovirus (CMV)/TetO2 promoter

    Available Since

    Feb. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA5/FRT/TO SBBP-CDK1 WT (2007)

    Plasmid

    #61847

    Purpose

    mammalian expression of CDK1 WT, used for Ips

    Depositor

    Jonathon Pines

    Insert

    CDK1 (CDK1 Human)
    

    Use

    Tags

    SBP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

16. pcDNA3 Flag-MBD1 R44Q-14-mVenusC

    Plasmid

    #191394

    Purpose

    R44Q binding pocket mutant of MBD domain of MBD1 fused to C-terminal part of split mVenus

    Depositor

    Albert Jeltsch

    Article

    Lungu et al Nat Commun. 2017 Sep 21;8(1):649. doi: 1

    Insert

    pcDNA3 Flag-MBD1 R44Q-14-mVenusC
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    R44Q

    Promoter

    CMV

    Available Since

    Dec. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCDNA3 Flag p38 gamma (agf)

    Plasmid

    #20354

    Depositor

    Roger Davis

    Article

    Enslen et al J Biol Chem. 1998 Jan 16. 273(3):1741-8.

    Insert

    pDCNA3 Flag p38 gamma(agf) (Mapk12 Rat)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Replace Thr 183 and Tyr 185 with Ala and Phe resp…

    Promoter

    Available Since

    April 29, 2009

    Availability

    Academic Institutions and Nonprofits only

18. pcDNA3.1(+)-Myr-DnaJB1-PKAcat-mCherry

    Plasmid

    #181853

    Purpose

    mCherry-tagged DnaJB1-PKA catalytic subunit fusion with N-terminal myristoylation site added; for mammalian expression.

    Depositor

    Jin Zhang

    Article

    Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.

    Insert

    Myr-DnaJB1-PKAcat-mCherry (PRKACA, DNAJB1 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    July 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA3-Flag mTOR S2035T R2109A

    Plasmid

    #26606

    Depositor

    Jie Chen

    Article

    Vilella-Bach et al J Biol Chem. 1999 Feb 12. 274(7):4266-72

    Insert

    mTOR (MTOR Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    S2035T R2109A

    Promoter

    Available Since

    Nov. 18, 2010

    Availability

    Academic Institutions and Nonprofits only

20. pcDNA3-Myc3-Cul3 H2M/H5M

    Plasmid

    #21591

    Depositor

    Yue Xiong

    Article

    Furukawa et al Mol Cell Biol. 2005 Jan . 25(1):162-71.

    Insert

    cullin 3 (CUL3 Human)
    

    Use

    Tags

    myc3

    Expression

    Mammalian

    Mutation

    Mutated residues H2M/H5M

    Promoter

    Available Since

    July 1, 2009

    Availability

    Academic Institutions and Nonprofits only