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pECFP_C1_NSD2 FL

Plasmid

#221382

Purpose

Expresses NSD2 in mammalian cells

Depositor

Albert Jeltsch

Article

Weirich et al Commun Biol. 2024 Jun 8;7(1):707. doi: 1

Insert

Histone-lysine N-methyltransferase NSD2 (NSD2 Human)

Expression

Mammalian

Mutation

L237R mutation in ECFP (Please see depositor comm…

Promoter

CMV

Available Since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

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pDG330

Plasmid

#100898

Purpose

SpCas9 with a cloning backbone for 2 custom gRNAs which can be cloned in via a one-step reaction. For generation of double knock-outs and large deletions in a single plasmid system.

Depositor

Paul Thomas

Article

Adikusuma et al PLoS One. 2017 Dec 6;12(12):e0187236. do

Inserts

humanized CRISPR associated protein 9 Nickase
U6-gRNA scaffold 1
U6-gRNA scaffold 2

Use

CRISPR and Mouse Targeting

Tags

3xFLAG

Expression

Mammalian

Promoter

CBh and U6

Available Since

Dec. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pEF1a-hMLH1NTD-NLS

Plasmid

#174826

Purpose

Mammalian expression of human MLH1NTD-NLS (codon optimized)

Depositor

David Liu

Article

Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

Insert

human MLH1_NTD-NLS (codon optimized)

Tags

SV40 bpNLS

Expression

Mammalian

Mutation

truncation of residues 1-335

Promoter

EF1a

Available Since

Oct. 14, 2021

Availability

Academic Institutions and Nonprofits only

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His-GFP-Clathrin

Plasmid

#170858

Purpose

Mammalian expression of His-GFP-Clathrin.

Depositor

Justin Taraska

Article

Prasai et al Nat Commun. 2021 Jun 25;12(1):3970. doi:

Insert

Clathrin light polypeptide (Lca) (Clta Mouse)

Tags

(His)6 and EGFP

Expression

Mammalian

Promoter

CMV

Available Since

June 22, 2021

Availability

Academic Institutions and Nonprofits only

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pST_119_LVL2 cam

Plasmid

#179333

Purpose

NT-CRISPR plasmid for integration of multiple gRNAs.

Depositor

Anke Becker

Article

Stukenberg et al Commun Biol. 2022 Mar 25;5(1):265. doi:

Insert

Ptac tfoX, Ptet cas9, PJ23106 acrIIA4, sfGFP dropout to be replaced with gRNAs

Use

CRISPR

Available Since

March 17, 2022

Availability

Academic Institutions and Nonprofits only

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1b34

Plasmid

#177692

Purpose

HCV E1E2 expression plasmid

Depositor

Justin Bailey

Article

Salas et al Gastroenterology. 2022 Feb;162(2):562-57

Insert

HCV E1E2

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pGL4_10xUAS-MLPmin-luc2

Plasmid

#194383

Purpose

Reporter plasmid for split TEV assays; firefly luciferase reporter gene driven by 10x clustered UAS binding sites linked to a minimal MLP promoter

Depositor

Michael Wehr

Article

Wu et al Biosensors (Basel). 2022 Dec 29;13(1):48

Insert

10xUAS-luc2

Expression

Mammalian

Promoter

MLP minimal

Available Since

Jan. 30, 2023

Availability

Academic Institutions and Nonprofits only

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EGFP-CLC

Plasmid

#193559

Purpose

Expresses human Clathrin Light Chain B tagged with mEGFP in mammalian cells.

Depositor

Justin Taraska

Article

Obashi et al Nat Commun. 2023 Feb 9;14(1):732. doi: 1

Insert

Clathrin light chain B (CLTB Human)

Tags

mEGFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-FLEx-tdT-P2A-Mxra8

Plasmid

#242478

Purpose

AAV vector expressing Mxra8 and tdTomato under cre-recombinase control

Depositor

Justus Kebschull

Article

Kim et al bioRxiv 2025.06.25.661405; doi: https://

Inserts

Mxra8 (Mxra8 Mouse)
tdTomato

Use

AAV and Cre/Lox

Tags

FLAG

Expression

Mammalian

Available Since

Sept. 18, 2025

Availability

Academic Institutions and Nonprofits only

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1a72

Plasmid

#177690

Purpose

HCV E1E2 expression plasmid

Depositor

Justin Bailey

Article

Salas et al Gastroenterology. 2022 Feb;162(2):562-57

Insert

HCV E1E2

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

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1b25

Plasmid

#177691

Purpose

HCV E1E2 expression plasmid

Depositor

Justin Bailey

Article

Salas et al Gastroenterology. 2022 Feb;162(2):562-57

Insert

HCV E1E2

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 24, 2021

Availability

Academic Institutions and Nonprofits only

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1b58

Plasmid

#177693

Purpose

HCV E1E2 expression plasmid

Depositor

Justin Bailey

Article

Salas et al Gastroenterology. 2022 Feb;162(2):562-57

Insert

HCV E1E2

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA pDEST40 Cas9-POLD3

Plasmid

#183194

Purpose

Expression clone

Depositor

Emma Haapaniemi

Article

Reint et al Elife. 2021 Dec 13;10. pii: 75415. doi:

Insert

POLD3

Use

CRISPR

Tags

Cas9WT

Expression

Mammalian

Mutation

n/a

Available Since

May 6, 2022

Availability

Academic Institutions and Nonprofits only

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CLC-EGFP

Plasmid

#193566

Purpose

Expresses human Clathrin Light Chain B tagged with mEGFP in mammalian cells.

Depositor

Justin Taraska

Article

Obashi et al Nat Commun. 2023 Feb 9;14(1):732. doi: 1

Insert

Clathrin light chain B (CLTB Human)

Tags

mEGFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 10, 2023

Availability

Academic Institutions and Nonprofits only

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His-GFP-FCHo2

Plasmid

#170870

Purpose

Mammalian expresssion of His-GFP-FCHo2.

Depositor

Justin Taraska

Article

Prasai et al Nat Commun. 2021 Jun 25;12(1):3970. doi:

Insert

FCH domain only 2 (Fcho2) (Fcho2 Mouse)

Tags

(His)6 and mEmerald

Expression

Mammalian

Promoter

CMV

Available Since

June 22, 2021

Availability

Academic Institutions and Nonprofits only

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GST-3C-FIP200-GFP

Plasmid

#187832

Purpose

Plasmid for the expression and purification of GST-3C-FIP200-GFP. Internal reference: SMC1445

Depositor

Sascha Martens

Article

Turco et al Nat Commun. 2021 Sep 1;12(1):5212. doi:

Insert

FIP200 (RB1CC1 Human)

Tags

GST and mGFP

Expression

Bacterial and Insect

Available Since

Oct. 20, 2022

Availability

Academic Institutions and Nonprofits only

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pCRISPaint-T2A-Gal4-3xP3-RFP

Plasmid

#127556

Purpose

CRISPaint universal donor plasmid for gene exon insertion. Encodes T2A-Gal4-SV40 and 3xP3-RFP visible eye marker.

Depositor

Norbert Perrimon

Article

Bosch et al Genetics. 2019 Nov 4. pii: genetics.119.

Insert

Gal4

Use

CRISPR

Expression

Insect

Available Since

Aug. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pJJGL001

Plasmid

#180605

Purpose

Plasmid expressing E. coli codon optimized His-MBP-TEV-DpbCasX

Depositor

Jennifer Doudna

Article

Tsuchida et al Mol Cell. 2022 Mar 17;82(6):1199-1209.e6

Insert

DpbCasX

Use

CRISPR

Tags

10x His and MBP

Expression

Bacterial

Promoter

T7

Available Since

April 25, 2022

Availability

Academic Institutions and Nonprofits only

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1a154 (H77)

Plasmid

#177689

Purpose

HCV E1E2 expression plasmid

Depositor

Justin Bailey

Article

Salas et al Gastroenterology. 2022 Feb;162(2):562-57

Insert

HCV E1E2

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pTorPE-R-GECO1

Plasmid

#32465

Depositor

Robert Campbell

Article

Zhao et al Science. 2011 Sep 30;333(6051):1888-91.

Insert

R-GECO1.0

Use

Lab constructed

Tags

6x His and Modified TorA MNNNDLFQASRRRFLAQLG[G to…

Expression

Bacterial

Mutation

Substitutions relative to the mApple-derived anal…

Available Since

Sept. 29, 2011

Availability

Academic Institutions and Nonprofits only

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