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Plasmid#236770PurposeA sleeping beauty-based vector containing CAG promoter-driven mCherry-P2A-EIF3D S528D/S529D-IRES-Pac.DepositorInsertEIF3D (EIF3D Human)
UseSleeping beautyTagsExpressionMammalianMutationchanged Serine 528 to Aspartic Acid and Serine 52…PromoterCAG promoterAvailable sinceMay 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
SB-CAG-mCherry-P2A-EIF3D-NN-IP
Plasmid#236771PurposeA sleeping beauty-based vector containing CAG promoter-driven mCherry-P2A-EIF3D S528N/S529N-IRES-Pac.DepositorInsertEIF3D (EIF3D Human)
UseSleeping beautyTagsExpressionMammalianMutationchanged Serine 528 to Asparagine and Serine 529 t…PromoterCAG promoterAvailable sinceMay 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
pT2/SV-Venus-SIINFEKL
Plasmid#111624PurposeSleeping Beauty transposon containing SV-40-driven Venus-LEQLE-SIINFEKL-TEW for antigen presentation studiesDepositorInsertVenus-LEQLE-SIINFEKL-TEW
UseTagsLEQLE-SIINFEKL-TEWExpressionMammalianMutationPromoterSV40Available sinceJune 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSBBi-Pur LaG17-synNotch-TetRVP64
Plasmid#158133PurposeConstitutive expression of anti-GFP nanobody (LaG-17) synNotch TetRVP64 receptor in a pSBBi-Pur Sleeping Beauty backboneDepositorInsertLaG17-synNotch-TetRVP64
UseTransposonTagsExpressionMammalianMutationPromoterEF1αAvailable sinceJune 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSBBi-Pur LaG17-synNotch-Gal4VP64
Plasmid#158135PurposeConstitutive expression of anti-GFP nanobody (LaG-17) synNotch Gal4VP64 receptor in a pSBBi-Pur Sleeping Beauty backboneDepositorInsertLaG17-synNotch-Gal4VP64
UseTransposonTagsExpressionMammalianMutationPromoterEF1αAvailable sinceOct. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pT/CAGGS-mVenus-P2A-NRASG12V
Plasmid#236072PurposeSleeping Beauty transposon plasmid for hydrodynamic tail vein injection (HDTVi) into mice. Caggs promotor driven expression of mVenus and mutant NRAS (NRASG12V).DepositorInsertsUseSleeping beauty transposon plasmidTagsExpressionMutationG12VPromoterCAGGSAvailable sinceMay 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pT/UBC-mVenus-P2A-NRASG12V
Plasmid#236074PurposeSleeping Beauty transposon plasmid for hydrodynamic tail vein injection (HDTVi) into mice. Ubc promotor driven expression of mVenus and mutant NRAS (NRASG12V).DepositorInsertsUseSleeping beauty transposon plasmidTagsExpressionMutationG12VPromoterUbcAvailable sinceMay 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pT/CAGGS-NRASG12V/D38A-IRES-mVenus
Plasmid#236077PurposeSleeping Beauty transposon plasmid for hydrodynamic tail vein injection (HDTVi) into mice. Caggs promotor driven expression of mVenus and a double mutant NRAS (NRASG12VD38A).DepositorInsertsUseSleeping beauty transposon plasmidTagsExpressionMutationG12V D38APromoterCAGGSAvailable sinceMay 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pT/CAGGS-NRASG12V-IRES-mVenus
Plasmid#236076PurposeSleeping Beauty transposon plasmid for hydrodynamic tail vein injection (HDTVi) into mice. Caggs promotor driven expression of mVenus and mutant NRAS (NRASG12V).DepositorInsertsUseSleeping beauty transposon plasmidTagsExpressionMutationG12VPromoterCAGGSAvailable sinceMay 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pT/PGK-mVenus-P2A-NRASG12V
Plasmid#236073PurposeSleeping Beauty transposon plasmid for hydrodynamic tail vein injection (HDTVi) into mice. Pgk promotor driven expression of mVenus and mutant NRAS (NRASG12V).DepositorInsertsUseSleeping beauty transposon plasmidTagsExpressionMutationG12VPromoterPGKAvailable sinceMay 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pSBbi-RP Hu CXCR3
Plasmid#183255PurposeExpression of human CXCR3 in a Sleeping Beauty transposon vectorDepositorInsertCXCR3 (CXCR3 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT2/shαSMA/GFP4_Seq1/clone1
Plasmid#206138PurposeKnockdown of alpha-SMA. Construct has inverted repeats to be used in Sleeping Beauty transposon system.DepositorInsertalpha-SMA (Acta2 Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pT3-Neo-EF1a-GFP
Plasmid#69134PurposeSleeping Beaquty (SB) vector encoding G418 resistance and GFP from two separate promotersDepositorTypeEmpty backboneUseSleeping beauty transposonTagsExpressionMammalianMutationPromoterU3MSCVAvailable sinceJan. 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSB_PhiC31LandingSite
Plasmid#48875PurposeSleeping beauty transposon with the PhiC31 landing site in a cmlc2::EGFP selection cassetteDepositorInsertcmlc2::attP-EGFP
UseTagsExpressionMutationPromoterAvailable sinceNov. 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
pTS395_PhCMV-SB100
Plasmid#169633PurposeExpression vector encoding PhCMV driven SB100 sleeping beauty transposase (PhCMV-SB100-pA)DepositorInsertPCMV-driven SB100
UseTagsExpressionMammalianMutationPromoterPhCMVAvailable sinceJune 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUHD-SB-mAID/Zeo
Plasmid#171681PurposeTRE-controlled mAID-fusion sleeping beauty transposon vector with selection marker (zeocin)DepositorTypeEmpty backboneUseTagsmAIDExpressionMammalianMutationPromoterTREAvailable sinceJuly 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUHD-SB-mAID/Bla
Plasmid#171680PurposeTRE-controlled mAID-fusion sleeping beauty transposon vector with selection marker (blasticidin)DepositorTypeEmpty backboneUseTagsmAIDExpressionMammalianMutationPromoterTREAvailable sinceJuly 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUHD-SB-AID/Bla
Plasmid#171678PurposeTRE-controlled AID-fusion sleeping beauty transposon vector with selection marker (blasticidin)DepositorTypeEmpty backboneUseTagsAIDExpressionMammalianMutationPromoterTREAvailable sinceJuly 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUHD-SB-AID/Hyg
Plasmid#171677PurposeTRE-controlled AID-fusion sleeping beauty transposon vector with selection marker (hygromycin)DepositorTypeEmpty backboneUseTagsAIDExpressionMammalianMutationPromoterTREAvailable sinceJuly 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSBDEST.IP
Plasmid#79472PurposePromoterless, Sleeping Beauty-compatible Gateway destination vector, IRES PuromycinDepositorTypeEmpty backboneUsePromoterless gateway destination vectorTagsExpressionMutationPromoternoneAvailable sinceMay 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pUHD-SB-mAID/Hyg
Plasmid#171679PurposeTRE-controlled mAID-fusion sleeping beauty transposon vector with selection marker (hygromycin)DepositorInsertmAID
UseTagsExpressionMammalianMutationPromoterTREAvailable sinceJuly 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSBDEST.B
Plasmid#79460PurposePromoterless, Sleeping Beauty-compatible Gateway destination vector, Blasticidin selectionDepositorTypeEmpty backboneUsePromoterless gateway destination vectorTagsExpressionMutationPromoternoneAvailable sinceMay 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSBDEST.Z
Plasmid#79470PurposePromoterless, Sleeping Beauty-compatible Gateway destination vector, ZeocinDepositorTypeEmpty backboneUsePromoterless gateway destination vectorTagsExpressionMutationPromoternoneAvailable sinceMay 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTS1107_Tier3(SB)
Plasmid#169634PurposeTier-3 vector for stable integration of up to three cassettes via sleeping beauty transposase (5'ITR-A1-pA::A2-pA::A3-pA-3'ITR)DepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceJune 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSBDEST.N
Plasmid#79468PurposePromoterless, Sleeping Beauty-compatible Gateway destination vector, NeomycinDepositorTypeEmpty backboneUsePromoterless gateway destination vectorTagsExpressionMutationPromoternoneAvailable sinceMay 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSBDEST.H
Plasmid#79464PurposePromoterless, Sleeping Beauty-compatible Gateway destination vector, Hygromycin selectionDepositorTypeEmpty backboneUsePromoterless gateway destination vectorTagsExpressionMutationPromoternoneAvailable sinceMay 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSBbi-TIR1/Pur
Plasmid#171682Purposeconstitutive expression of TIR1-myc in sleeping beauty transposon vector with selection marker (puromycin)DepositorInsertOsTIR1-MYC
UseTagsMYCExpressionMammalianMutationPromoterEF-1a promoterAvailable sinceOct. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSBbi-TIR1-tTA/Neo
Plasmid#171684Purposeconstitutive expression of TIR1-myc and tTA in sleeping beauty transposon vector with selection marker (G418)DepositorInsertNeomycin
UseTagsExpressionMammalianMutationPromoterRPBSA promoterAvailable sinceOct. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSBbi-TIR1-tTA/Zeo
Plasmid#171685Purposeconstitutive expression of TIR1-myc and tTA in sleeping beauty transposon vector with selection marker (zeocin)DepositorInsertZeocin
UseTagsExpressionMammalianMutationPromoterRPBSA promoterAvailable sinceOct. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSBbi-TIR1-tTA/Pur
Plasmid#171683Purposeconstitutive expression of TIR1-myc and tTA in sleeping beauty transposon vector with selection marker (puromycin)DepositorInsertstetracycline-controlled transactivator
Puromycin
EF-1a promoter
UseTagsExpressionMammalianMutationPromoterEF-1a promoter and RPBSA promoterAvailable sinceOct. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSBtet-2xMARS-nGFP-puro
Plasmid#205239PurposeDOX inducible expression of two repeats of PLEKHA5 aa 143-271 (K163A and R164A) fused to a HA-tagged GFP nanobody in mammalian cells from the Sleeping Beauty transposon systemDepositorInsert2xPLEKHA5(143-271)-K163A/R164A-HA-nGFP (PLEKHA5 Human)
UseTransposonTagsHA, Nuclear Export Sequence, mScarlet-i, and nGFP…ExpressionMammalianMutationamino acids 143-271 of PLEKHA5 (GenBank reference…Promotertight TRE promoterAvailable sinceJuly 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKT-mIDH1(R132H)-Myc-FLAG-IRES-Kat
Plasmid#133779PurposeExpresses IDH1 with a R132H mutation and has Myc and FLAG tags. IDH1(R132H) also has a katushka fluorescent reporter. Construct has inverted repeats to be used in Sleeping Beauty transposon system.DepositorInsertIsocitrate dehydrogenase 1 (IDH1 Human)
UseTagsMyc and FLAGExpressionMammalianMutationArginine is mutated to histidine at amino acid po…PromoterAvailable sinceOct. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
InterTag
Plasmid#186910PurposeSleeping Beauty transposon system with constitutive bi-directional promoter, one side expressing SpyTag003-sfCherry11 fused to PDGFR and the other side expressing BFP and puromycin resistance geneDepositorInsertSpyTag003-sfCherry11-PDGFR
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
InterTag-3xL-Δtag
Plasmid#186914PurposeSleeping Beauty transposon system with constitutive bi-directional promoter, expressing sfCherry11 fused to PDGFR through a (GGGGS)3 linker and the other side BFP and puromycin resistance geneDepositorInsertsfCherry11-(GGGGS)3-PDGFR
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
InterCatch
Plasmid#186906PurposeSleeping Beauty transposon system with constitutive bi-directional promoter, one side expressing SpyCatcher003-sfCherry1-10 fused to TfR and the other side expressing EGFP and puromycin resistance geneDepositorInsertTfR-SpyCatcher003-sfCherry1-10
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTC-ApoE-Tet
Plasmid#85578PurposeSleeping beauty transposon for hydrodynamic tail vein injection for CreER expression and tetracyclin-dependent transgene/shRNA expression (ApoE.HCR.hAAT promoter ) in mouse liverDepositorInsertCreER
UseCre/LoxTagsExpressionMammalianMutationPromoterApoE.HCR.hAATAvailable sinceFeb. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
InterCatch-3xL
Plasmid#186909PurposeSleeping Beauty transposon system with constitutive bi-directional promoter, one side expressing SpyCatcher003-sfCherry1-10 fused to TfR through (GGGGS)3 linker and the other side expressing EGFP and puromycin resistance geneDepositorInsertTfR-(GGGGS)3-SpyCatcher003-sfCherry1-10
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
InterTag-3xL
Plasmid#186913PurposeSleeping Beauty transposon system with constitutive bi-directional promoter, one side expressing SpyTag003-sfCherry11 fused to PDGFR through a (GGGGS)3 linker and the other side expressing BFP and puromycin resistance geneDepositorInsertSpyTag003-sfCherry11-(GGGGS)3-PDGFR
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
InterCatch-1xL
Plasmid#186907PurposeSleeping Beauty transposon system with constitutive bi-directional promoter, one side expressing SpyCatcher003-sfCherry1-10 fused to TfR through a single GGGGS linker and the other side expressing EGFP and puromycin resistance geneDepositorInsertTfR-(GGGGS)1-SpyCatcher003-sfCherry1-10
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDBEcR/Bla
Plasmid#201556PurposePlasmid with SB ITRs for expressing DBEcR (3xmyc-tagged) under EF1α promoter; blasticidin resistance.DepositorInsertDBEcR
UseUnspecified; Sleeping beauty transposonTagsMycExpressionMammalianMutationPromoterEF1aAvailable sinceJuly 13, 2023AvailabilityAcademic Institutions and Nonprofits only