We narrowed to 2,475 results for: T2A

1. pBabe_Zeo_cOVA_T2A_mStrawberry

   Plasmid

   #161736

   Purpose

   Expression of cOVA and mStrawberry

   Depositor

   Alec Kimmelman

   Article

   Yamamoto et al Nature. 2020 May;581(7806):100-105. doi:

   Insert

   cytoplasmic ovalbumin
   

   Use

   Retroviral

   Tags

   mStrawberry

   Expression

   Mammalian

   Available Since

   Dec. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. PB_TetON_mCherry_P2AT2A_GFP-nb

   Plasmid

   #238239

   Purpose

   For integration of mCherry_P2AT2A_GFP-nb

   Depositor

   Denes Hnisz

   Article

   Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

   Insert

   mCherry_P2AT2A_GFP-nb
   

   Expression

   Mammalian

   Available Since

   June 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pH2rU3_ForInd_Omicron_sinobiological_Spiked21_T7_CMV_ZsGT2APurR

   Plasmid

   #204146

   Purpose

   Lentivirus backbone with BA.1 spike under an inducible promoter and constitutive expressed zsGreen-T2A-PuR genes

   Depositor

   Jesse Bloom

   Article

   Dadonaite et al Cell. 2023 Mar 16;186(6):1263-1278.e20.

   Inserts

   SARS-CoV-2 BA.1 variant spike
   zsGreen-T2A-PuR
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   21 amino acid cytoplasmic tail deletion

   Promoter

   CMV and TRE3G

   Available Since

   Aug. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pHR_Gal4UAS_Tbet_T2A_GFP_PGK_mCherry

   Plasmid

   #85431

   Purpose

   Lentiviral vector for Gal4 inducible Tbet T2A GFP and constitutive mCherry expression

   Depositor

   Wendell Lim

   Article

   Roybal et al Cell. 2016 Oct 6;167(2):419-432.e16. doi

   Insert

   human Tbet
   

   Use

   Lentiviral

   Available Since

   Oct. 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pH2rU3_ForInd_sinobiological_617.2_Spiked21_CMV_ZsGT2APurR

   Plasmid

   #204151

   Purpose

   Lentivirus backbone with Delta spike under an inducible promoter and constitutive expressed zsGreen-T2A-PuR genes

   Depositor

   Jesse Bloom

   Article

   Dadonaite et al Cell. 2023 Mar 16;186(6):1263-1278.e20.

   Insert

   SARS-CoV-2 Delta variant spike
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   21 amino acid cytoplasmic tail deletion

   Promoter

   TRE3G

   Available Since

   Aug. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. PB_iMYOD1_P2A_BAF60C2_T2A_mCh_Puro

   Plasmid

   #168807

   Purpose

   PiggyBac transposon construct with doxycycline inducible MYOD1, BAF60C + mCherry expression

   Depositor

   Prashant Mali

   Article

   Dailamy et al Stem Cell Reports. 2021 Sep 8. pii: S221

   Insert

   MYOD1 (MYOD1 Human, Synthetic)
   

   Use

   Synthetic Biology; Piggybac

   Expression

   Mammalian

   Promoter

   TRE

   Available Since

   Aug. 31, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pRK5_TagBFP_P2AT2A_GFP-nb-KS

   Plasmid

   #238274

   Purpose

   For overexpression of TagBFP_P2AT2A_GFP-nb-KS

   Depositor

   Denes Hnisz

   Article

   Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

   Insert

   TagBFP_P2AT2A_GFP-nb-KS
   

   Expression

   Mammalian

   Available Since

   June 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pRK5_TagBFP_P2AT2A_GFP-nb

   Plasmid

   #238273

   Purpose

   For overexpression of TagBFP_P2AT2A_GFP-nb

   Depositor

   Denes Hnisz

   Article

   Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

   Insert

   TagBFP_P2AT2A_GFP-nb
   

   Expression

   Mammalian

   Available Since

   June 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pLenti_Cas9_T2A_mNeonGreen_P2A_blasticidin

   Plasmid

   #211471

   Purpose

   lentiviral tricistronic complex expressing Cas9, mNeonGreen, and blasticidin resistance

   Depositor

   James Eshleman

   Article

   Teh et al NAR Cancer. 2024 Jun 19;6(2):zcae028. do

   Insert

   Cas9_T2A_mNeonGreen_P2A_BlasticidinR
   

   Use

   CRISPR and Lentiviral

   Tags

   3xFlag

   Expression

   Mammalian

   Promoter

   hU6, EF-1a

   Available Since

   March 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pEX.sEF1a.Kozak.HA.NLS.Sce.T2A.BFP

    Plasmid

    #45565

    Purpose

    Expresses I-Sce I nuclease in mammalian cells and BFP as a tracker

    Depositor

    Andrew Scharenberg

    Article

    Kuhar et al Nucleic Acids Res. 2013 Oct 10.

    Inserts

    I-Sce I Nuclease
    BFP
    

    Tags

    HA

    Expression

    Mammalian

    Available Since

    Nov. 4, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pEX.d14GFP.sEF1a.Kozak.HA.NLS.Sce.T2A.BFP

    Plasmid

    #45564

    Purpose

    Expresses I-Sce I nuclease and BFP tracker in mammalian cells, codes for eGFP donor sequence

    Depositor

    Andrew Scharenberg

    Article

    Kuhar et al Nucleic Acids Res. 2013 Oct 10.

    Inserts

    d14 eGFP donor
    I-Sce I Nuclease
    BFP
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    deleted n' 15 amino acids

    Available Since

    Nov. 4, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. CDK1as_T2A_Zeo

    Plasmid

    #118596

    Purpose

    One of three plasmids required to create CDK1as human cells by single transfection (One shot system). CDK1as cDNA and Zeocin resistance cassette flanked by Sleeping Beauty transposon.

    Depositor

    William Earnshaw

    Article

    Saldivar et al Science. 2018 Aug 24;361(6404):806-810.

    Insert

    CDK1
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pT2ADWpuro_2paRAF

    Plasmid

    #129653

    Purpose

    For generating cell line expressing the 2P-activatable RAF system with P2A and full-length RAF1

    Depositor

    Michiyuki Matsuda

    Article

    Kinjo et al Nat Methods. 2019 Sep 9. pii: 10.1038/s4

    Insert

    2paCRY2-Raf1-2A-CIBN-mScarlet-KRasCAAX
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Sept. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. PCYT2A

    Plasmid

    #42415

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    PCYT2A (PCYT2 Human)
    

    Tags

    His6-TEV

    Expression

    Bacterial

    Mutation

    See Comments

    Available Since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

15. XClone_Cas9_KRAB_T2A_BFP

    Plasmid

    #141208

    Purpose

    Tunable and temporal expression control of dCas9

    Depositor

    Rory Johnson

    Insert

    dCas9
    

    Expression

    Mammalian

    Available Since

    Aug. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. PX458_3XHA_den1FnCas9_KRAB_T2A_eGFP

    Plasmid

    #200910

    Purpose

    3XHA tagged dead en1Cas9 from F. novicida with KRAB repressor domain, T2A self-cleaving peptide and enhanced GFP biomarker for mammalian expression

    Depositor

    Debojyoti Chakraborty

    Insert

    3XHA_den1FnCas9_KRAB_eGFP
    

    Use

    CRISPR

    Tags

    3xHA-SV40NLS and Nucleoplasmin NLS-KRAB-T2A-EGFP

    Expression

    Mammalian

    Mutation

    Changed Glutamic Acid to Arginine at 1369 position

    Promoter

    CBH

    Available Since

    Sept. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. MT2a

    Plasmid

    #105693

    Purpose

    Bacterial expression plasmids for production of recombinant metallothionein 2a

    Depositor

    Artur Krężel

    Article

    Drozd et al Metallomics. 2018 Apr 25;10(4):595-613.

    Insert

    Metallothionein 2a (MT2A Human)
    

    Tags

    Chiting Binding Domanin-Intein

    Expression

    Bacterial

    Available Since

    April 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. KAT2A

    Plasmid

    #38914

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    KAT2A (KAT2A Human)
    

    Tags

    His Tev

    Expression

    Bacterial

    Available Since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

19. pT2ADW_2paRAF

    Plasmid

    #129654

    Purpose

    For generating Tg mice expressing the 2P-activatable RAF system with P2A and full-length RAF1

    Depositor

    Michiyuki Matsuda

    Article

    Kinjo et al Nat Methods. 2019 Sep 9. pii: 10.1038/s4

    Insert

    2paCRY2-Raf1-2A-CIBN-mScarlet-KRasCAAX
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Sept. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pWPI_GW_BioID2_T2A_Blast

    Plasmid

    #214831

    Purpose

    Destination vector for cloning BioID2 fusion proteins

    Depositor

    Hani Goodarzi

    Article

    Khoroshkin et al Nat Commun. 2024 Sep 9;15(1):7872. doi:

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    BioID2

    Expression

    Bacterial and Mammalian

    Available Since

    Aug. 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge