We narrowed to 3,485 results for: T2A

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pBS_KS_attB2_SA(2)_T2ALexA

Plasmid

#125222

Purpose

Artificial phase 2 exon to include a spliced T2A-LexA effector into a locus of interest through RMCE of MiMIC insertion in an intron between coding exons of a gene

Depositor

Herman Dierick

Article

Gnerer et al Nucleic Acids Res. 2015 Apr 30;43(8):e56

Insert

LexA

Use

Other

Tags

T2A

Available Since

May 16, 2019

Availability

Academic Institutions and Nonprofits only

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KAT2A sgRNA2

Plasmid

#138186

Purpose

3rd generation lentiviral gRNA plasmid targeting human KAT2A

Depositor

Wen Xue

Article

Kwan et al Hepatology. 2019 Jun 12. doi: 10.1002/he

Insert

KAT2A (KAT2A Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

March 18, 2020

Availability

Academic Institutions and Nonprofits only

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KAT2A sgRNA1

Plasmid

#138185

Purpose

3rd generation lentiviral gRNA plasmid targeting human KAT2A

Depositor

Wen Xue

Article

Kwan et al Hepatology. 2019 Jun 12. doi: 10.1002/he

Insert

KAT2A (KAT2A Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

March 18, 2020

Availability

Academic Institutions and Nonprofits only

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OSC_Reporter_UAS_traffic jam_BoxB_d2eGFP_t2a_Blast

Plasmid

#128010

Purpose

traffic jam enhancer driven GFP_P2A-Blasticidin-resistance harboring 10 intronic boxB sites and 14 upstream UAS sites

Depositor

Julius Brennecke

Article

Batki et al Nat Struct Mol Biol. 2019 Aug;26(8):720-

Insert

traffic jam enhancer driven GFP_P2A-Blasticidin-resistance harboring 10 intronic boxB sites and 14 upstream UAS sites

Use

Drosophila osc reporter construct

Available Since

July 25, 2019

Availability

Academic Institutions and Nonprofits only

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pME18ST-d5-HT2A

Plasmid

#53109

Purpose

Expression of Drosophila 5-HT2A

Depositor

Leslie Vosshall

Article

Gasque et al Sci Rep. 2013;3:srep02120. doi: 10.1038/

Insert

5HT2A (5-HT2 Fly)

Available Since

June 24, 2014

Availability

Academic Institutions and Nonprofits only
pBS_KS_attB2_SA(0)_T2AGAL4

Plasmid

#125211

Purpose

Artificial phase 0 exon to include a spliced T2A-GAL4 effector into a locus of interest through RMCE of MiMIC insertion in an intron between coding exons of a gene

Depositor

Herman Dierick

Article

Gnerer et al Nucleic Acids Res. 2015 Apr 30;43(8):e56

Insert

GAL4

Use

Other

Tags

T2A

Available Since

May 15, 2019

Availability

Academic Institutions and Nonprofits only

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pBS_KS_attB2_SA(1)_T2AGAL4

Plasmid

#125212

Purpose

Artificial phase 1 exon to include a spliced T2A-GAL4 effector into a locus of interest through RMCE of MiMIC insertion in an intron between coding exons of a gene

Depositor

Herman Dierick

Article

Gnerer et al Nucleic Acids Res. 2015 Apr 30;43(8):e56

Insert

GAL4

Use

Other

Tags

T2A

Available Since

May 15, 2019

Availability

Academic Institutions and Nonprofits only

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pBS_KS_attB2_SA(2)_T2AGAL4

Plasmid

#125213

Purpose

Artificial phase 2 exon to include a spliced T2A-GAL4 effector into a locus of interest through RMCE of MiMIC insertion in an intron between coding exons of a gene

Depositor

Herman Dierick

Article

Gnerer et al Nucleic Acids Res. 2015 Apr 30;43(8):e56

Insert

GAL4

Use

Other

Tags

T2A

Available Since

May 15, 2019

Availability

Academic Institutions and Nonprofits only

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MAT2AA-c001

Plasmid

#53648

Purpose

bacterial expression plasmid

Depositor

Nicola Burgess-Brown

Insert

MAT2AA

Tags

His6-TEV

Expression

Bacterial

Mutation

Bacterial expression for structure determination;…

Available Since

June 3, 2014

Availability

Academic Institutions and Nonprofits only
786 pCSmAKT2Acherry

Plasmid

#31167

Depositor

Nathan Lawson

Article

Covassin et al Dev Biol. 2009 May 15. 329(2):212-26.

Insert

Akt

Use

Expression vector

Tags

Myr and mCherry

Mutation

membrane tethered Akt

Available Since

Sept. 6, 2011

Availability

Academic Institutions and Nonprofits only
pBS_KS_attB2_SA(0)_T2AGeneSwitch

Plasmid

#125214

Purpose

Artificial phase 0 exon to include a spliced T2A-GeneSwitch effector into a locus of interest through RMCE of MiMIC insertion in an intron between coding exons of a gene

Depositor

Herman Dierick

Article

Gnerer et al Nucleic Acids Res. 2015 Apr 30;43(8):e56

Insert

GeneSwitch

Use

Other

Tags

T2A

Available Since

May 15, 2019

Availability

Academic Institutions and Nonprofits only

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PB-Haunt E1-PuroT2AmCherry-4A KI

Plasmid

#92164

Purpose

For lncRNA 4_pA(3_SV40 polyA and 1 BGH polyA) homologue recombination mediated knockin

Depositor

Xiaohua Shen

Article

Yin et al Cell Stem Cell. 2015 May 7;16(5):504-16.

Insert

Haunt P-Inr2-cDNA KI homology arms

Use

Other

Available Since

March 29, 2019

Availability

Academic Institutions and Nonprofits only

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MT2a_K30A K31A

Plasmid

#105696

Purpose

Bacterial expression plasmids for production of recombinant metallothionein 2a K30/31A mutant

Depositor

Artur Krężel

Article

Singh et al Metallomics. 2023 Jun 1;15(6):mfad029. d

Insert

Metallothionein 2a (MT2A Human)

Tags

Chiting Binding Domanin-Intein

Expression

Bacterial

Mutation

Lysine 30 and 31 to Alanine

Available Since

April 27, 2020

Availability

Academic Institutions and Nonprofits only

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pBS_KS_attB2_SA(2)_T2AGeneSwitch

Plasmid

#125216

Purpose

Artificial phase 2 exon to include a spliced T2A-GeneSwitch effector into a locus of interest through RMCE of MiMIC insertion in an intron between coding exons of a gene

Depositor

Herman Dierick

Article

Gnerer et al Nucleic Acids Res. 2015 Apr 30;43(8):e56

Insert

GeneSwitch

Use

Other

Tags

T2A

Available Since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pBS_KS_attB2_SA(2)_T2AGAL80

Plasmid

#125219

Purpose

Artificial phase 2 exon to include a spliced T2A-GAL80 effector into a locus of interest through RMCE of MiMIC insertion in an intron between coding exons of a gene

Depositor

Herman Dierick

Article

Gnerer et al Nucleic Acids Res. 2015 Apr 30;43(8):e56

Insert

GAL80

Use

Other

Tags

T2A

Available Since

June 28, 2019

Availability

Academic Institutions and Nonprofits only

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pBS_KS_attB2_SA(1)_T2AGeneSwitch

Plasmid

#125215

Purpose

Artificial phase 1 exon to include a spliced T2A-GeneSwitch effector into a locus of interest through RMCE of MiMIC insertion in an intron between coding exons of a gene

Depositor

Herman Dierick

Article

Gnerer et al Nucleic Acids Res. 2015 Apr 30;43(8):e56

Insert

GeneSwitch

Use

Other

Tags

T2A

Available Since

May 16, 2019

Availability

Academic Institutions and Nonprofits only

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pT2A11 (GB0143)

Plasmid

#68189

Purpose

Provides the S. Lycopersicum 2A11 terminator as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

Sl2A11 terminator

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

Oct. 23, 2015

Availability

Academic Institutions and Nonprofits only

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pCAG-dCas9-5xPlat2AflD

Plasmid

#82560

Purpose

Expression vector for Cas9 peptide array (linker length: 22aa).

Depositor

Izuho Hatada

Article

Morita et al Nat Biotechnol. 2016 Aug 29. doi: 10.103

Insert

dCas9-5xPlat2AflD

Expression

Mammalian

Available Since

Sept. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pUAS:Cas9T2AGFP;U6:sgRNA1;U6:sgRNA2

Plasmid

#74009

Purpose

Tissue-specific knock-out in zebrafish, Cas9 and GFP driven by a UAS promoter

Depositor

Filippo Del Bene

Article

Di Donato et al Genome Res. 2016 Mar 8.

Inserts

5x UAS
Cas9
T2A
GFP
U6 promoter

Use

CRISPR

Available Since

April 20, 2016

Availability

Academic Institutions and Nonprofits only

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pPB-R1R2_EF1aVP64dCas9VP64_T2A_MS2p65HSF1-IRESbsdpA

Plasmid

#113341

Purpose

Expresses VP64-dCas9-VP64 and MS2-p65-HSF1 fusion proteins

Depositor

Gavin Wright

Article

Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:

Insert

VP64-dCas9-VP64-T2A-MS2-p65-HSF1

Use

Piggybac

Expression

Mammalian

Available Since

Dec. 12, 2018

Availability

Academic Institutions and Nonprofits only

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