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We narrowed to 57,437 results for: cloning

Showing: 41 - 60 of 57437 results

  1. EasyCloneYALI- CRISPR/Cas9 Set

    Plasmid Kit
    #1000000141
    Purpose
    Contains plasmids for genome editing of Y. lipolytica via CRISPR/Cas9
    Depositor
    Irina Borodina
    Available since
    Jan. 3, 2019
    Availability
    Academic Institutions and Nonprofits only

  2. The Expanded EasyClone-MarkerFree toolkit

    Plasmid Kit
    #1000000190
    Purpose
    The Expanded EasyClone-MarkerFree toolkit is a new set of 8 validated chromosomal loci for the stable integration of heterologous DNA into baker’s yeast Saccharomyces cerevisiae.
    Depositor
    Irina Borodina
    Available since
    Aug. 9, 2022
    Availability
    Academic Institutions and Nonprofits only

  3. Escherichia coli DH10B-MOB

    Bacterial Strain
    #229395
    Purpose
    Escherichia coli DH10B DAP-auxotrophic strain containing an integrated RK2/RP4 mobilization vector to conjugate plasmids with an IncP origin of transfer (modified pTA-Mob 2.0, AddGene Plasmid #149662)
    Depositor
    Michael Springer
    Article
    Soltysiak et al ACS Synth Biol. 2025 Apr 18;14(4):1173-1
    Bacterial Resistance
    60 ug/ml of dap
    Species
    Escherichia coli
    Available since
    March 24, 2025
    Availability
    Academic Institutions and Nonprofits only

  4. RAS Pathway 2.0 Clone Collection

    Plasmid Kit
    #1000000070
    Purpose
    Collection contains 180 genes in the “RAS pathway version 2.0”, a crowdsourced pathway map of the RAS signaling pathway; For use with Gateway cloning or FNLCR Combinatorial Cloning Platform
    Depositor
    Dominic Esposito
    Available since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only

  7. TB202

    Bacterial Strain
    #230032
    Purpose
    Fluorescently labelled (mCerulean) E. coli
    Depositor
    Calin Guet, Tobias Bergmiller
    Bacterial Resistance
    None
    Available since
    Feb. 3, 2025
    Availability
    Academic Institutions and Nonprofits only

  13. XClone_Cas9_KRAB_T2A_BFP

    Plasmid
    #141208
    Purpose
    Tunable and temporal expression control of dCas9
    Depositor
    Rory Johnson
    Insert
    dCas9
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 18, 2020
    Availability
    Academic Institutions and Nonprofits only
    141208-plasmid-map-sequence-id-308770

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  14. psglessClonePX

    Plasmid
    #87193
    Purpose
    used for downstream cloning, contains CMV promoter with NheI and XhoI cloning sites flanking a partial insert that gets excised when adding an insert of choice
    Depositor
    Thomas Cech
    Article
    Goldfarb et al Genes Dev. 2017 Jan 1;31(1):59-71. doi:
    Type
    Empty backbone
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 24, 2017
    Availability
    Academic Institutions and Nonprofits only
    87193-plasmid-map-sequence-id-170165

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  19. pPEPZ-sgRNAclone

    Plasmid
    #141090
    Purpose
    This vector is designed for efficient cloning of sgRNAs by Golden Gate assembly. The sgRNA insertion leads to replacement of mCherry, resulting in loss of red color of E. coli colony.
    Depositor
    Jan-Willem Veening
    Article
    Liu et al Cell Host Microbe. 2021 Jan 13;29(1):107
    Insert
    mCherry
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    p3
    Available since
    April 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    141090-plasmid-map-sequence-id-330475

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Showing: 41 - 60 of 57437 results