We narrowed to 6,951 results for: crispr cas9 plasmids

1. pAAVS1-NDi-CRISPRd (ABI-dCas9-mCherry)

   Plasmid

   #216531

   Purpose

   Targeting vector for CRISPRd generation

   Depositor

   Makiko Iwafuchi

   Article

   Matsui et al Mol Cell. 2024 Jan 4:S1097-2765(23)01026

   Insert

   ABI-dCas9-mCherry
   

   Use

   CRISPR

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   TRE3G

   Available since

   Sept. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. BPK4410 - human expression plasmid for SpCas9 Cluster 1 (HypaCas9)

   Plasmid

   #101178

   Purpose

   Human expression plasmid for SpCas9 Cluster 1 variant (HypaCas9): CMV-T7-hSpCas9-Cluster1(N692A, M694A, Q695A, H698A)-NLS(SV40)-3xFLAG

   Depositor

   Jennifer Doudna, Keith Joung

   Article

   Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

   Insert

   hSpCas9-Cluster1(N692A/M694A/Q695A/H698A)
   

   Use

   Tags

   3x FLAG and NLS (SV40)

   Expression

   Mammalian

   Mutation

   N692A/M694A/Q695A/H698A

   Promoter

   CMV

   Available since

   Sept. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJSC114 - Bacterial expression plasmid for SpCas9 eSpCas9(1.1) variant

   Plasmid

   #101215

   Purpose

   Bacterial expression plasmid for SpCas9 eSpCas9(1.1) variant

   Depositor

   Jennifer Doudna, Keith Joung

   Article

   Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

   Insert

   SpCas9 variant K848A/K1003A/R1060A
   

   Use

   Tags

   10x His, MBP, and TEV site

   Expression

   Bacterial

   Mutation

   K848A, K1003A and R1060A

   Promoter

   T7

   Available since

   Oct. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJSC173 - Bacterial expression plasmid for SpCas9 Cluster 1 (HypaCas9) variant

   Plasmid

   #101218

   Purpose

   Bacterial expression plasmid for SpCas9 Cluster 1 (HypaCas9) variant

   Depositor

   Jennifer Doudna, Keith Joung

   Article

   Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

   Insert

   SpCas9 variant N692A/M694A/Q695A/H698A
   

   Use

   Tags

   10x His, MBP, and TEV site

   Expression

   Bacterial

   Mutation

   N692A, M694A, Q695A and H698A

   Promoter

   T7

   Available since

   Nov. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJSC270 - Bacterial expression plasmid for SpCas9 eSpCas9(1.1)-HF1 variant

   Plasmid

   #101217

   Purpose

   Bacterial expression plasmid for SpCas9 eSpCas9(1.1)-HF1 variant

   Depositor

   Jennifer Doudna, Keith Joung

   Article

   Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

   Insert

   SpCas9 variant K848A/K1003A/R1060A/N497A/R661A/Q695A/Q926A
   

   Use

   Tags

   10x His, MBP, and TEV site

   Expression

   Bacterial

   Mutation

   K848A, K1003A, R1060A, N497A, R661A, Q695A and Q9…

   Promoter

   T7

   Available since

   Oct. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJSC281 - Bacterial expression plasmid for SpCas9 Cluster 1 (HypaCas9), REC2 FRET variant

   Plasmid

   #101230

   Purpose

   Bacterial expression plasmid for SpCas9 Cluster 1 (HypaCas9), REC2 FRET variant

   Depositor

   Jennifer Doudna, Keith Joung

   Article

   Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

   Insert

   SpCas9 variant C80S/C574S/E60C/D273C/N692A/M694A/Q695A/H698A
   

   Use

   Tags

   10x His, MBP, and TEV site

   Expression

   Bacterial

   Mutation

   C80S, C574S, E60C, D273C, N692A, M694A, Q695A and…

   Promoter

   T7

   Available since

   Nov. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJSC282 - Bacterial expression plasmid for SpCas9 Cluster 1 (HypaCas9), REC3 FRET variant

   Plasmid

   #101231

   Purpose

   Bacterial expression plasmid for SpCas9 Cluster 1 (HypaCas9), REC3 FRET variant

   Depositor

   Jennifer Doudna, Keith Joung

   Article

   Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

   Insert

   SpCas9 variant C80S/C574S/S701C/S960C/N692A/M694A/Q695A/H698A
   

   Use

   Tags

   10x His, MBP, and TEV site

   Expression

   Bacterial

   Mutation

   C80S, C574S, S701C, S960C, N692A, M694A, Q695A an…

   Promoter

   T7

   Available since

   Nov. 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJSC011 - Bacterial expression plasmid for SpCas9 Cluster 1 (HypaCas9), HNH FRET variant

   Plasmid

   #101229

   Purpose

   Bacterial expression plasmid for SpCas9 Cluster 1 (HypaCas9), HNH FRET variant

   Depositor

   Jennifer Doudna, Keith Joung

   Article

   Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

   Insert

   SpCas9 variant C80S/C574S/S355C/S867C/N692A/M694A/Q695A/H698A
   

   Use

   Tags

   10x His, MBP, and TEV site

   Expression

   Bacterial

   Mutation

   C80S, C574S, S355C, S867C, N692A, M694A, Q695A an…

   Promoter

   T7

   Available since

   Oct. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRP[2CRISPR]-mCherry/Hygro-hCas9-U6>{mdx left}-U6>{mdx right}

   Plasmid

   #184381

   Purpose

   This plasmid contains hCas9 and two gRNAs that can excise the genomic area around the mouse mdx mutation in dystrophin's exon 23

   Depositor

   Ori Bar-Nur

   Article

   Domenig et al Stem Cell Reports. 2022 Feb 8;17(2):321-

   Insert

   Cas9, two gRNAs targeting dystrophin
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. Jun lab tunable CRISPRi strain

    Bacterial Strain

    #86400

    Purpose

    Strain SJ_XTL219 Genotype: MG1655 PBAD_dcas9 ΔlacI ΔaraE araFGH<>spec lacY A177C, galM<pBBa-J23119-tet-sacB-handle-term

    Depositor

    Suckjoon Jun

    Article

    Li et al Sci Rep. 2016 Dec 20;6:39076. doi: 10.10

    Bacterial Resistance

    Spectinomycin and hygromycin

    Available since

    Feb. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

11. pSHS207 - Bacterial expression plasmid for WT SpCas9

    Plasmid

    #101199

    Purpose

    Bacterial expression plasmid for WT SpCas9

    Depositor

    Jennifer Doudna, Keith Joung

    Article

    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

    Insert

    SpCas9
    

    Use

    Tags

    10x His, MBP, and TEV site

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pRP[CRISPR]-EGFP/Puro-hCas9-U6>(long left)-U6>(long right)

    Plasmid

    #216871

    Purpose

    This plasmid contains hCas9 and two gRNAs that can excise the genomic area around the mouse mdx mutation in dystrophin's exon 23

    Depositor

    Ori Bar-Nur

    Article

    Lenardic et al J Clin Invest. 2024 May 7:e166998. doi:

    Insert

    Cas9, gRNA Long.L, gRNA Long.R (Dmd Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    mdx mutation in mouse dystrophin

    Promoter

    Available since

    June 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. dCas9 plasmid

    Plasmid

    #100091

    Purpose

    dCas9 expression plasmid without effector fusions; 3X Flag tag; 2NLS; pCDNA3 vector backbone, mammalian expression

    Depositor

    David Segal

    Article

    O'Geen et al Nucleic Acids Res. 2017 Sep 29;45(17):99

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    3XFlag-NLS-dCas9-NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. BPK3258 - human expression plasmid for eSpCas9(1.1)

    Plasmid

    #101176

    Purpose

    Human expression plasmid for SpCas9 eSpCas9(1.1) variant: CMV-T7-hSpCas9-eSpCas9(1.1)(K848A, K1003A, R1060A)-NLS(SV40)-3xFLAG

    Depositor

    Jennifer Doudna, Keith Joung

    Article

    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

    Insert

    hSpCas9-eSpCas9(1.1)(K848A/K1003A/R1060A)
    

    Use

    Tags

    3x FLAG and NLS (SV40)

    Expression

    Mammalian

    Mutation

    K848A/K1003A/R1060A

    Promoter

    CMV

    Available since

    Sept. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. BPK4387 - human expression plasmid for SpCas9 Cluster 5

    Plasmid

    #101191

    Purpose

    Human expression plasmid for SpCas9 Cluster 5 variant: CMV-T7-hSpCas9-Cluster5(K918A, V922A, R925A)-NLS(SV40)-3xFLAG

    Depositor

    Jennifer Doudna, Keith Joung

    Article

    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

    Insert

    hSpCas9-Cluster5(K918A/V922A/R925A)
    

    Use

    Tags

    3x FLAG and NLS (SV40)

    Expression

    Mammalian

    Mutation

    K918A/V922A/R925A

    Promoter

    CMV

    Available since

    Sept. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. MMW3645 - human expression plasmid for SpCas9 Cluster 3

    Plasmid

    #101187

    Purpose

    Human expression plasmid for SpCas9 Cluster 3 variant: CMV-T7-hSpCas9-Cluster3(T657A, G658A, W659A, R661A)-NLS(SV40)-3xFLAG

    Depositor

    Jennifer Doudna, Keith Joung

    Article

    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

    Insert

    hSpCas9-Cluster3(T657A/G658A/W659A/R661A)
    

    Use

    Tags

    3x FLAG and NLS (SV40)

    Expression

    Mammalian

    Mutation

    T657A/G658A/W659A/R661A

    Promoter

    CMV

    Available since

    Sept. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. MMW3770 - human expression plasmid for SpCas9 Cluster 4

    Plasmid

    #101189

    Purpose

    Human expression plasmid for SpCas9 Cluster 4 variant: CMV-T7-hSpCas9-Cluster4(F491A, M495A, T496A, N497A)-NLS(SV40)-3xFLAG

    Depositor

    Jennifer Doudna, Keith Joung

    Article

    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

    Insert

    hSpCas9-Cluster4(F491A/M495A/T496A/N497A)
    

    Use

    Tags

    3x FLAG and NLS (SV40)

    Expression

    Mammalian

    Mutation

    F491A/M495A/T496A/N497A

    Promoter

    CMV

    Available since

    Sept. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. MMW2993 - human expression plasmid for SpCas9 Cluster 2

    Plasmid

    #101185

    Purpose

    Human expression plasmid for SpCas9 Cluster 2 variant: CMV-T7-hSpCas9-Cluster2(G582A, V583A, E584A, D585A, N588A)-NLS(SV40)-3xFLAG

    Depositor

    Jennifer Doudna, Keith Joung

    Article

    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

    Insert

    hSpCas9-Cluster2(G582A/V583A/E584A/D585A/N588A)
    

    Use

    Tags

    3x FLAG and NLS (SV40)

    Expression

    Mammalian

    Mutation

    G582A/V583A/E584A/D585A/N588A

    Promoter

    CMV

    Available since

    Sept. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. CRISPR plasmid

    Plasmid

    #236553

    Purpose

    Encodes Cas9 and two guideRNAs, one to cut the genome downstream of HSPA5 (BiP/GRP78) and one to linearize the DONOR plasmid.

    Depositor

    Pablo Rivera-Fuentes

    Insert

    Cas9
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pJSC111 - Bacterial expression plasmid for SpCas9-HF1 variant

    Plasmid

    #101209

    Purpose

    Bacterial expression plasmid for SpCas9-HF1 variant

    Depositor

    Jennifer Doudna, Keith Joung

    Article

    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

    Insert

    SpCas9 variant N497A/R661A/Q695A/Q926A
    

    Use

    Tags

    10x His, MBP, and TEV site

    Expression

    Bacterial

    Mutation

    N497A, R661A, Q695A and Q926A

    Promoter

    T7

    Available since

    Nov. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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