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Plasmid#187190PurposeProtein ExpressionDepositorInsertENSDARG00000069463 (alox12 Zebrafish)
UseTagsEGFPExpressionBacterialMutationPromoterAvailable sinceAug. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pOC5 cloning template vector
Plasmid#183424PurposeFlpOFF knock-in vectorDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceApril 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pOC3 cloning template vector
Plasmid#183422PurposeCreON FlpOFF knock-in vectorDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceApril 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pORANGE LOX Cloning template vector
Plasmid#139651PurposeCloning template for ORANGE-CAKE conditional knock-in constructsDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterU6Available sinceApril 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
hU6-pegRNA-EFS-mScarlet Template
Plasmid#196037PurposeThe template UPEmS vector designed for cloning of a single pegRNA and subsequent co-expression with mScarlet.DepositorInsertmScarlet
UseTagsExpressionMammalianMutationPromoterhU6 and EFSAvailable sinceJune 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBx1-VHH template1-3XMyc-Spacer
Plasmid#176208PurposePCR template for generating CeVICA VHH/nanobody libraryDepositorInsertVHH template1-3XMyc-Spacer
UseTagsMyc tagExpressionBacterialMutationPromoterT7Available sinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBx1-VHH template3-3XMyc-Spacer
Plasmid#176210PurposePCR template for generating CeVICA VHH/nanobody libraryDepositorInsertVHH template3-3XMyc-Spacer
UseTagsMyc tagExpressionBacterialMutationPromoterT7Available sinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBx1-VHH template2-3XMyc-Spacer
Plasmid#176209PurposePCR template for generating CeVICA VHH/nanobody libraryDepositorInsertVHH template2-3XMyc-Spacer
UseTagsMyc tagExpressionBacterialMutationPromoterT7Available sinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
human SLC12A5-V5-HA-2A-Luciferase repair template
Plasmid#158576PurposeFor targeted insertion of V5-HA-2A-Luciferase reporter before the stop codon of the human SLC12A5 gene that encodes neuronal chloride transporter KCC2 protein.DepositorInserthuman SLC12A5 5' homology arm-V5-HA-2A-Luciferase-3' homology arm (SLC12A5 Human)
UseTagsV5 and HA on the endogenous KCC2 protein productExpressionMammalianMutationPromoterAvailable sinceSept. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPB-CAG-PlatTET-wpA-pPuroTK
Plasmid#214125PurposeGene expression by Piggybac transposase in mammalian cellsDepositorInsertdCas9-TET
UseTagsExpressionMammalianMutationCodon-optimized for mammalian cellsPromoterAvailable sinceFeb. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGL3-noSV40-EC1.45-human_min1-platypus_min2
Plasmid#173967PurposeFirefly luciferase enhancer reporter plasmid.DepositorInsertHuman minimal enhancer region 1 (min1) and platypus minimal enhancer region 2 (min2) from SOX9 enhancer cluster EC1.45
UseLuciferaseTagsExpressionMutationNonePromoterSV40 promoterAvailable sinceSept. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPD027 Reporter Template v1 (Near Spaced)
Plasmid#138751PurposeTemplate for COMET promoters, expresses EYFP reporter upon promoter activation.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPD032 Reporter Template v1 (Mid Spaced)
Plasmid#138753PurposeTemplate for COMET promoters, expresses EYFP reporter upon promoter activation.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPD033 Reporter Template v1 (Far Spaced)
Plasmid#138754PurposeTemplate for COMET promoters, expresses EYFP reporter upon promoter activation.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGL3-Basic-Rab18-sfGFP(N) HDR template
Plasmid#129415PurposeHDR tempalte for tagging of endogenous human RAB18 N-terminus with sfGFPDepositorInsertRAB18 HDR template (RAB18 Human)
UseCRISPR and TALEN; Endogenous tagging hdr templateTagssfGFPExpressionMammalianMutationPromoterAvailable sinceAug. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
SOX9-T2A-H2B-EGFP repair template
Plasmid#167972PurposeSOX9 reporter repair templateDepositorInsertSRY-Box Transcription Factor 9 (SOX9 Human)
UseCRISPR; Donor templateTagsH2B-EGFPExpressionMammalianMutationhomology arms contain point mutations to disrupt …PromoterAvailable sinceJune 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGL3-Basic-ACSL3-sfGFP(C) HDR template
Plasmid#129413PurposeHDR tempalte for tagging of endogenous human ACSL3 C-terminus with sfGFPDepositorInsertACSL3 HDR template (ACSL3 Human)
UseCRISPR and TALEN; Endogenous tagging hdr templateTagssfGFPExpressionMammalianMutationPromoterAvailable sinceAug. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
mNeonGreen-P2A-3xFLAG-hEEA1 HDR template
Plasmid#214989PurposeEndogenously tag human EEA1 with an N-terminal 3xFLAG in addition to cytosolic mNG for cell sorting.DepositorInsertEEA1 (EEA1 Human)
UseCRISPRTags3xFLAGExpressionMutationPromoterAvailable sinceMay 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
mNeonGreen-P2A-3xFLAG-msEEA1 HDR template
Plasmid#214990PurposeEndogenously tag mouse EEA1 with an N-terminal 3xFLAG in addition to cytosolic mNG for cell sorting.DepositorInsertEEA1 (Eea1 Mouse)
UseCRISPRTags3xFLAGExpressionMutationPromoterAvailable sinceApril 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUC57-2xFLAG-mAID-AMBRA1 HDR donor template
Plasmid#172609PurposeDonor plasmid containing the HDR template for the in-frame, N-terminal 2xFLAG-mAID tagging of AMBRA1 geneDepositorInsert2xFLAG-mAID-AMBRA1 (AMBRA1 Synthetic, Human)
UseCRISPR; Human targetingTagsExpressionMutationPromoterAvailable sinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only