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We narrowed to 169 results for: primer

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Showing: 41 - 60 of 169 results

  1. pUC_sgRNA

    Plasmid
    #68710
    Purpose
    Contains the sgRNA backbone sequence (tracrRNA, 82 bp) and is used as DNA template to amplify a specific sgRNA using a forward primer with the protospacer sequence for gene targeting.
    Depositor
    Roberto Docampo
    Publication
    Lander et al MBio. 2015 Jul 21;6(4). pii: e01012-15.
    Type
    Empty backbone
    Use
    CRISPR and Synthetic Biology
    Available since
    Sept. 14, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    5 Articles
    68710-plasmid-map-sequence-id-123992

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  2. pGZ110 (pFA6a-3FLAG-MNase-TRP1)

    Plasmid
    #70233
    Purpose
    ChEC-tagging vector encoding a 3xFLAG tag and aa 83-231 of Mnase in pFA6a-3HA-Trp1 replacing the 3xHA tag. These vectors retain compatibility with the F2/R1 primer pairs commonly used for epitope tag
    Depositor
    Steven Henikoff
    Publication
    Zentner et al Nat Commun. 2015 Oct 22;6:8733. doi: 10.
    Insert
    3xFLAG tag and aa 83-231 of Mnase
    Use
    Yeast genomic targeting
    Available since
    Nov. 6, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    70233-plasmid-map-sequence-id-129168

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  4. pc5139-pEGFP-H3.1-K36M

    Plasmid
    #241430
    Purpose
    Expresses human Histone H3.1 as fusion to GFP with lysine to methionine point mutation of K36 from plasmid H3.1 using primers: forward: ACCGGTGGCGTGATGAAACCTCATCGC & reverse: GCGATGAGGTTTCATC
    Depositor
    Cristina Cardoso
    Publication
    Pradhan et al EMBO Rep. 2025 Oct;26(20):4950-4976. doi
    Insert
    human Histone H3.1 (H3C1 Human)
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    lysine at position 36 to methionine (K36M)
    Promoter
    CMV
    Available since
    June 1, 2026
    Availability
    Academic Institutions and Nonprofits only
    241430-plasmid-map-sequence-id-484514

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  7. pc2099-pEGFP-H3.1

    Plasmid
    #241429
    Purpose
    Expresses human Histone H3.1 as fusion to GFP constructed by PCR using Primers: h3.1-cl-fw (AAGAATTCAATGGCTCGTACGAAGCAAAC) and h3.1-cl-rv (AAGCGGCCGCTGCCCTTTCCCCACGGATG) cloned into pEGFP-C1
    Depositor
    Cristina Cardoso
    Publication
    Pradhan et al EMBO Rep. 2025 Oct;26(20):4950-4976. doi
    Insert
    human Histone H3.1 (H3C1 Human)
    Tags
    EGFP
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    June 1, 2026
    Availability
    Academic Institutions and Nonprofits only
    241429-plasmid-map-sequence-id-484515

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  8. CROPseq-3P5P

    Plasmid
    #219680
    Purpose
    CROPseq vector enabling gRNA detection via mRNA-based capture (CROPseq), 3'-based direct capture using 10x capture sequence 1 or 5'-based enrichment using a custom reverse transcription primer
    Depositor
    Christoph Bock
    Publication
    Traxler et al Cell Syst. 2025 Aug 20;16(8):101346. doi
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Tags
    EF1a-Puro-P2A-mCherry2-WPRE-hU6-gRNA
    Expression
    Mammalian
    Available since
    Feb. 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    219680-plasmid-map-sequence-id-462884

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Showing: 41 - 60 of 169 results