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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 25,973 results for: his

Showing: 581 - 600 of 25973 results

  1. Ntng1.c-Fc-His

    Plasmid
    #72112
    Purpose
    Expresses the extracellular region of the Netrin G1, isoform c protein (truncated immediately upstream of propeptide cleavage and GPI-attachement site), C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.
    Depositor
    Woj Wojtowicz
    Article
    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1
    Insert
    Ntng1.c (Ntng1 Mouse)
    Use
    Tags
    Fc-His
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    72112-plasmid-map-sequence-id-131298

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  3. pST50Trc2-FLAGHISNDHFR

    Plasmid
    #63965
    Purpose
    Position 2 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable double tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control
    Depositor
    Song Tan
    Article
    Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9
    Type
    Empty backbone
    Use
    Tags
    6xHis & FLAG; TEV cleavable
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Oct. 7, 2015
    Availability
    Academic Institutions and Nonprofits only
    63965-plasmid-map-sequence-id-177498

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  8. NLuc-His10

    Plasmid
    #234567
    Purpose
    Bacterially expressed NLuc for Ni-NTA purification
    Depositor
    James Inglese
    Article
    Ciulla et al Nat Commun. 2025 May 10;16(1):4342. doi:
    Insert
    Nano luciferase
    Use
    Tags
    T7 gene 10 leader peptide and Thrombin site-10xHi…
    Expression
    Bacterial
    Mutation
    Codon optimized for bacterial expression
    Promoter
    T7
    Available since
    May 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    234567-plasmid-map-sequence-id-466796

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  11. pET15bHis6-pG-Tnp

    Plasmid
    #124884
    Purpose
    To transform BL21-Gold(DE3) and induced by IPTG for His-proteinG-Tnp (hyperactive mutant) fusion protein.
    Depositor
    Keji Zhao
    Insert
    protein G's C1D1C2D2C3 domains contained in the protein G gBLOCK.
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    April 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    124884-plasmid-map-sequence-id-243752

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  12. pET15bHis6-pG-MNase

    Plasmid
    #124886
    Purpose
    To transform BL21-Gold(DE3) and be induced by IPTG for His-proteinG-MNase fusion protein expression.
    Depositor
    Keji Zhao
    Insert
    protein G-MNase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    April 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    124886-plasmid-map-sequence-id-243685

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  13. pML104-HIS3-3

    Plasmid
    #232882
    Purpose
    Plasmid expressing Cas9 and gRNA AAACCTTTTTACTCCACGCA which targets the HIS3 gene.
    Depositor
    Jolanda Van Leeuwen
    Insert
    HIS3 gRNA (HIS3 Budding Yeast)
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    snR52
    Available since
    Feb. 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    232882-plasmid-map-sequence-id-462144

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  14. pML104-HIS3-2

    Plasmid
    #232881
    Purpose
    Plasmid expressing Cas9 and gRNA CCAAGTTCGACAACTGCGTA which targets the HIS3 gene.
    Depositor
    Jolanda Van Leeuwen
    Insert
    HIS3 gRNA (HIS3 Budding Yeast)
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    snR52
    Available since
    Feb. 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    232881-plasmid-map-sequence-id-462138

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  15. pEND-443_Cacnes_HisB

    Plasmid
    #225608
    Purpose
    Suicide C. acnes vector to generate HisB (PPA_RS05815) deletion leaving an Ery resistance cassette flanked by BxbI recombination sites
    Depositor
    Marc Güell
    Article
    Guillermo et al Cell Systems, 2025
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Feb. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    225608-plasmid-map-sequence-id-451366

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Showing: 581 - 600 of 25973 results