We narrowed to 4,215 results for: spl

Showing: 581 - 600 of 4215 results

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pUBC_V5_MS2-mCherry

Plasmid

#68420

Purpose

Transient expression of humanized MS2 (V75E/A81G), fused to mCherry in mammalian cells, under a UBC promoter

Depositor

John Rinn

Publication

Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

Insert

MS2

Use

Synthetic Biology

Tags

V5 and mCherry

Expression

Mammalian

Mutation

MS2: V75E/A81G

Promoter

human UbC

Available since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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PX851

Plasmid

#62883

Purpose

N-term SpCas9 piece of inducible split-4 (Cas9(N)-FRB-NES split-4).

Depositor

Feng Zhang

Publication

Zetsche et al Nat Biotechnol. 2015 Feb 2. doi: 10.1038

Insert

SpCas9 (aa 2-535)

Use

CRISPR

Tags

FRB and NES PTK2

Expression

Mammalian

Promoter

CBh

Available since

March 4, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pcDNA3-FLAG-Luciferase

Plasmid

#58792

Purpose

Express FLAG-tagged Luciferase in mammalian cells

Depositor

Hiroyoshi Ariga

Publication

Maita et al Anal Biochem. 2014 Feb 3. pii: S0003-269

Insert

firefly Luciferase

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available since

Aug. 15, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles
2Flag-pDEST-C

Plasmid

#118372

Purpose

Gateway compatible mammalian expression vector with C-terminal 2Flag to tag proteins of interest.

Depositor

Maria Vartiainen

Publication

Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs

Type

Empty backbone

Use

Destination

Tags

2Flag

Expression

Mammalian

Available since

Nov. 28, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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PX852

Plasmid

#62884

Purpose

C-term SpCas9 piece of inducible split-4 (Cas9(C)-FKBP split-4).

Depositor

Feng Zhang

Publication

Zetsche et al Nat Biotechnol. 2015 Feb 2. doi: 10.1038

Insert

SpCas9 (aa536-1368)

Use

CRISPR

Tags

FKBP12, NLS SV40, and NLS nucleoplasmin

Expression

Mammalian

Promoter

CBh

Available since

March 4, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pCMV-Tag2B-NCreERT2

Plasmid

#106370

Purpose

N-terminal part of the split-CreERT2 System

Depositor

Johannes Hirrlinger

Publication

Hirrlinger et al PLoS One. 2009 Dec 16;4(12):e8354. doi:

Insert

NCreERT2

Use

Cre/Lox

Tags

flag

Expression

Mammalian

Promoter

CMV

Available since

March 20, 2018

Availability

Academic Institutions and Nonprofits only

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pXN22-Dest

Plasmid

#105085

Purpose

Yeast mating-based Split Ubiquitin Assay, prey vector for Gateway cloning

Depositor

Christopher Grefen

Publication

Grefen et al Curr Protoc Neurosci. 2007 Oct;Chapter 5

Type

Empty backbone

Tags

NubG-3xHA

Expression

Yeast

Available since

Feb. 14, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pTXB1-CfaC-Gyra

Plasmid

#122950

Purpose

Bacterial expression of C-intein

Depositor

Thomas Muir

Publication

Stevens et al J Am Chem Soc. 2016 Feb 24;138(7):2162-5

Insert

CfaC

Expression

Bacterial

Available since

April 18, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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SFG.CNb30_opt.IRES.eGFP

Plasmid

#22493

Depositor

Martin Pule

Publication

Brewin et al Blood. 2009 Nov 26;114(23):4792-803. doi

Insert

Codon optimised Calcineurin B mutant 30 (PPP3R1 Human)

Use

Retroviral

Mutation

L124T; K125-LA-Ins

Available since

Dec. 16, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pCMV14/3xFLAG-Pax3

Plasmid

#25427

Depositor

Jonathan Epstein

Insert

Pax3 (Pax3 Mouse)

Tags

3XFLAG

Expression

Mammalian

Available since

June 25, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
PX853

Plasmid

#62885

Purpose

N-term SpCas9 piece of inducible split-5 (Cas9(N)-FRB-NES split-5).

Depositor

Feng Zhang

Publication

Zetsche et al Nat Biotechnol. 2015 Feb 2. doi: 10.1038

Insert

SpCas9 (aa 2-573)

Use

CRISPR

Tags

FRB and NES PTK2

Expression

Mammalian

Promoter

CBh

Available since

March 5, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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AdML-M3

Plasmid

#11244

Depositor

Robin Reed

Publication

Zhou et al Proc Natl Acad Sci U S A. 2002 Sep 17. 9

Insert

AdML-M3

Tags

3 MS2 hairpins

Expression

Bacterial

Available since

April 20, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pCTCON2 Aga2P-HRP

Plasmid

#73152

Purpose

Full-length horseradish peroxidase (HRP) fused to the C-terminus of the yeast mating protein Aga2P. Galactose-inducible expression.

Depositor

Alice Ting

Publication

Martell et al Nat Biotechnol. 2016 May 30. doi: 10.103

Insert

Aga2P-HRP

Tags

HA tag and myc tag

Expression

Yeast

Promoter

GAL1

Available since

June 21, 2016

Availability

Academic Institutions and Nonprofits only

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pIVEX-SNAP-GFP

Plasmid

#46846

Purpose

SNAP-GFP fusion

Depositor

Florian Hollfelder

Publication

Diamante et al Protein Eng Des Sel. 2013 Oct;26(10):713

Insert

SNAP-GFP fusion

Use

In vitro expression

Available since

Aug. 12, 2013

Availability

Academic Institutions and Nonprofits only

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pEGFP-hG9a

Plasmid

#33025

Depositor

Martin Walsh

Publication

Nishio et al Proc Natl Acad Sci U S A. 2004 Aug 3;101

Insert

hG9a (EHMT2 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available since

April 2, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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PX854

Plasmid

#62886

Purpose

C-term SpCas9 piece of inducible split-5 (Cas9(C)-FKBP split-5).

Depositor

Feng Zhang

Publication

Zetsche et al Nat Biotechnol. 2015 Feb 2. doi: 10.1038

Insert

SpCas9 (aa573-1368)

Use

CRISPR

Tags

FKBP12, NLS SV40, and NLS nucleoplasmin

Expression

Mammalian

Promoter

CBh

Available since

March 16, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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FRB_SCF2_LD6_CTEV

Plasmid

#58879

Purpose

MESA split-TEV protease chain with Rapamycin FRB ectodomain 2 aa scaffold, 6 aa linker domain, C-terminal TEV protease

Depositor

Joshua Leonard

Publication

Daringer et al ACS Synth Biol. 2014 Mar 11.

Insert

MESA split protease chain with FRB rapamycin-binding ectodomain and C terminal fragment of TEV protease

Use

AAV

Expression

Mammalian

Promoter

CMV

Available since

Nov. 3, 2014

Availability

Academic Institutions and Nonprofits only

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pCAG-NL1A

Plasmid

#15227

Depositor

Peter Scheiffele

Publication

Chih et al Neuron. 2006 Jul 20. 51(2):171-8.

Insert

Neuroligin 1 with insert A (Nlgn1 Mouse)

Tags

HA

Expression

Mammalian

Available since

Jan. 23, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pCMV-Tag3B-ERT2CCre

Plasmid

#106371

Purpose

C-terminal part of the split-CreERT2 System

Depositor

Johannes Hirrlinger

Publication

Hirrlinger et al PLoS One. 2009 Dec 16;4(12):e8354. doi:

Insert

ERT2CCre

Use

Cre/Lox

Tags

c-myc

Expression

Mammalian

Promoter

CMV

Available since

March 20, 2018

Availability

Academic Institutions and Nonprofits only

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pLHCX-FLAG-mPKM1

Plasmid

#44240

Depositor

Lewis Cantley, Matthew Vander Heiden

Publication

Christofk et al Nature. 2008 Mar 13;452(7184):230-3. doi

Insert

Pyruvate Kinase M1 (Pkm Mouse)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available since

April 10, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles