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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 19,851 results for: Nov;

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Showing: 5981 - 6000 of 19851 results

  1. ARB365

    Plasmid
    #124048
    Purpose
    Encodes pEF1a driving expression of TET3G P2A iRFP713, pEF1a expressing sadCas9 fused to mRuby2 P2A mRuby2, and an sgRNA targeting pUAS expressing mAzamiGreen in a PiggyBac destination vector
    Depositor
    Hana El-Samad
    Article
    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2
    Insert
    PB_pEF1a-tet3G-P2A-iRFP713_pEF1a-sadCAS9::mRuby2-P2A-mRuby2-SV40PA_mu6-sgUAS_pUAS-NLS::mAzamiGreen-rgPA
    Expression
    Mammalian
    Available Since
    Dec. 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    124048-plasmid-map-sequence-id-252519

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  4. pYFAC-CH2

    Plasmid
    #168978
    Purpose
    AMA1 based expression in filamentous fungi pYFAC-pyrG carrying PalcA-T1-PalcS/M-T2-PaldA expression cassette; unique restriction site: PacI for PalcA, NotI for PalcS, AsiSI for PalcM, AscI for PaldA
    Depositor
    Yit Heng Chooi
    Article
    Hu et al Chem Sci. 2018 Nov 22;10(5):1457-1465. d
    Type
    Empty backbone
    Use
    Synthetic Biology; Heterologous expression in asp…
    Available Since
    Feb. 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    168978-plasmid-map-sequence-id-353766

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  7. pLKO_HA.V5.FLAG_NGFR

    Plasmid
    #158250
    Purpose
    Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).
    Depositor
    Brian Brown
    Article
    Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d
    Insert
    Pro-Code Tagged human dNGFR (NGFR Human)
    Use
    CRISPR and Lentiviral
    Tags
    HA.V5.FLAG
    Mutation
    Truncation of the signaling domain
    Promoter
    EF1a
    Available Since
    Sept. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    158250-plasmid-map-sequence-id-327836

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  11. pcDNA5-FRT-TO-FH-Nsp13_GC3opt

    Plasmid
    #157715
    Purpose
    mammalian expression of N-terminally Flag-His8 tagged SARS-CoV-2 Nsp13 under control of a tetracycline-inducible promoter
    Depositor
    David Tollervey
    Article
    Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi
    Insert
    FH-Nsp13_GC3opt (ORF1ab SARS-CoV-2)
    Tags
    Flag-His8
    Expression
    Mammalian
    Mutation
    codon optimized; gene expression was optimized by…
    Available Since
    Aug. 6, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    157715-plasmid-map-sequence-id-307906

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  12. pcDNA5-FRT-TO-Nsp8-Nsp7-HF_GC3opt

    Plasmid
    #157702
    Purpose
    mammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 Nsp8-Nsp7 fusion protein under control of a tetracycline-inducible promoter
    Depositor
    David Tollervey
    Article
    Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi
    Insert
    Nsp8-Nsp7-HF_GC3opt (ORF1ab SARS-CoV-2)
    Tags
    His8-Flag
    Expression
    Mammalian
    Mutation
    codon optimized; gene expression was optimized by…
    Available Since
    Aug. 5, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    157702-plasmid-map-sequence-id-307866

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  16. pAAV-TRE-flex-ChR2 (E123T/T159C)-EYFP

    Plasmid
    #135176
    Purpose
    AAV expression of TRE(TRE3G)-driven, Cre-dependent, humanized channelrhodopsin E123T/T159C mutant fused to EYFP for optogenetic activation.
    Depositor
    Tetsuo Yamamori
    Article
    Ebina et al Proc Natl Acad Sci U S A. 2019 Nov 5;116
    Inserts
    hChR2
    TRE3G
    Use
    AAV and Cre/Lox
    Tags
    EYFP (C terminal on insert)
    Expression
    Mammalian
    Mutation
    E123T and T159C
    Promoter
    TRE3G
    Available Since
    Jan. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    135176-plasmid-map-sequence-id-268585

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  18. AAV2_hSyn_Aurora_Citrine

    Plasmid
    #98217
    Purpose
    Red-shifted artificial anion conducting channelrhodopsin (aACR). Activation up to 600 nm; off-kinetics 260 ms. Codon optimized for mammalian expression.
    Depositor
    Simon Wiegert
    Article
    Wietek et al Sci Rep. 2017 Nov 2;7(1):14957. doi: 10.
    Insert
    Synthetic construct Aurora gene
    Use
    AAV
    Tags
    Citrine
    Expression
    Mammalian
    Mutation
    V59S, E83N, E90Q, E101S, V117R, E123S, P242R, A24…
    Promoter
    human synapsin
    Available Since
    Aug. 22, 2017
    Availability
    Academic Institutions and Nonprofits only
    98217-plasmid-map-sequence-id-256049

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  19. AAV2_hSyn_Phobos_Citrine

    Plasmid
    #98216
    Purpose
    Blue-shifted artificial anion conducting channelrhodopsin (aACR). Activation max 466 nm; off-kinetics 10 ms. Codon optimized for mammalian expression.
    Depositor
    Simon Wiegert
    Article
    Wietek et al Sci Rep. 2017 Nov 2;7(1):14957. doi: 10.
    Insert
    Synthetic construct Phobos gene
    Use
    AAV
    Tags
    Citrine
    Expression
    Mammalian
    Mutation
    T59S, E83N, E90Q, E101S, V117R, E123S, T159G, G16…
    Promoter
    human synapsin
    Available Since
    Aug. 22, 2017
    Availability
    Academic Institutions and Nonprofits only
    98216-plasmid-map-sequence-id-256050

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Showing: 5981 - 6000 of 19851 results