We narrowed to 32,650 results for: Eng

1. pMT-sox10-cytoBirA-2A-mCherry_Ras

   Plasmid

   #80062

   Purpose

   Sox10 promoter driving HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences

   Depositor

   Tatjana Sauka-Spengler

   Article

   Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

   Insert

   Biotin ligase (BirA), a Thosea asigna virus peptide (2A), and mCherry protein
   

   Use

   Zebrafish biotagging

   Tags

   3x HA

   Expression

   Bacterial

   Promoter

   Sox10 promoter

   Available Since

   Oct. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAC94-pmax-dCas9VP160-2A-puro

   Plasmid

   #48226

   Purpose

   dCas9VP160-2A-puro (puro-selectable) on pmax expression vecor. Note: This is being tested.

   Depositor

   Rudolf Jaenisch

   Article

   Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

   Insert

   dCas9(D10A;H840A) fusion with VP160 activation domain followed by 2A-puro
   

   Use

   CRISPR

   Tags

   2A-puro, HA Tag, and VP160

   Expression

   Mammalian

   Mutation

   D10A;H840A (catalytically inactive)

   Promoter

   CAGGS

   Available Since

   Sept. 17, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLV V5-lamin A

   Plasmid

   #175107

   Purpose

   Lentiviral expression of V5-tagged mouse Lmna

   Depositor

   Larry Gerace

   Article

   Cheng et al J Proteome Res. 2022 Sep 2;21(9):2197-22

   Insert

   Lmna (Lmna Mouse)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Available Since

   Jan. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. xCas12i

   Plasmid

   #195333

   Purpose

   vector for encoding a human codon-optimized xCas12i driven by CBh promoter, guide RNAs compatible with xCas12i driven by hU6, and mCherry driven by CMV promoter

   Depositor

   Yingsi Zhou

   Article

   Zhang et al Protein Cell. 2023 Jun 28;14(7):538-543.

   Insert

   human codon-optimized xCas12i
   

   Tags

   3xFlag

   Expression

   Mammalian

   Promoter

   CBh, CMV, hU6

   Available Since

   Jan. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. sg_Gemin2_i1-ipUSEPR-TR657

   Plasmid

   #228919

   Purpose

   Knockdown of Gemin2 in mammalian cells

   Depositor

   Sika Zheng

   Article

   Lin et al Neuron. 2024 Jul 3;112(13):2157-2176.e12

   Insert

   sg_Gemin2_i1 (Gemin2 Mouse)
   

   Use

   Lentiviral

   Available Since

   Jan. 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. sg_Gemin2_i5-ipUSEPR-TR657

   Plasmid

   #228920

   Purpose

   Knockdown of Gemin2 in mammalian cells

   Depositor

   Sika Zheng

   Article

   Lin et al Neuron. 2024 Jul 3;112(13):2157-2176.e12

   Insert

   sg_Gemin2_i5 (Gemin2 Mouse)
   

   Use

   Lentiviral

   Available Since

   Jan. 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. sg_Snrpa_i2-ipUSEPR-TR657

   Plasmid

   #228923

   Purpose

   Knockdown of Snrpa in mammalian cells

   Depositor

   Sika Zheng

   Article

   Lin et al Neuron. 2024 Jul 3;112(13):2157-2176.e12

   Insert

   sg_Snrpa_i2 (Snrpa Mouse)
   

   Use

   Lentiviral

   Available Since

   Jan. 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. sg_Snrpa_i4-ipUSEPR-TR657

   Plasmid

   #228924

   Purpose

   Knockdown of Snrpa in mammalian cells

   Depositor

   Sika Zheng

   Article

   Lin et al Neuron. 2024 Jul 3;112(13):2157-2176.e12

   Insert

   sg_Snrpa_i4 (Snrpa Mouse)
   

   Use

   Lentiviral

   Available Since

   Jan. 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. AAV-GFAP-TurboID

   Plasmid

   #227179

   Purpose

   Co-expression of FLAG-tagged-TurboID and T2A-mCherry under the GFAP short promoter by AAV vector

   Depositor

   Junmin Peng

   Article

   Sun et al Anal Chem. 2022 Apr 5;94(13):5325-5334.

   Insert

   TurboID
   

   Use

   AAV

   Tags

   FLAG and T2A-mCherry

   Expression

   Mammalian

   Promoter

   GFAP_short

   Available Since

   Oct. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. H3S10ph biosensor

    Plasmid

    #120809

    Purpose

    FRET biosensor. To monitor histone H3 Serine 10 phosphorylation in mammalian cells by FRET

    Depositor

    Yingxiao Wang

    Article

    Peng et al Proc Natl Acad Sci U S A. 2018 Nov 26. p

    Insert

    Mouse histone H3-CFP-FHA2-histone H3 peptide (1-14aa)-YFP
    

    Expression

    Mammalian

    Mutation

    Threonine 3, Threonine 6, and Threonine 11 are mu…

    Promoter

    CMV

    Available Since

    Feb. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.1-Flag-mRIPK1-S321E

    Plasmid

    #115340

    Purpose

    Mammalian expression of Flag-mRIPK1-S321E

    Depositor

    Junying Yuan

    Article

    Geng et al Nat Commun. 2017 Aug 25;8(1):359. doi: 1

    Insert

    RIPK1 (Ripk1 Mouse)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    S321E

    Available Since

    Sept. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pENTR221 UBR5 ∆UBR

    Plasmid

    #81063

    Purpose

    Gateway entry vector encoding full length UBR5 W1235L (∆UBR)

    Depositor

    Darren Saunders

    Article

    Shearer et al Mol Biol Cell. 2018 May 9:mbcE17040248.

    Insert

    Ubiquitin protein ligase E3 component N-recognin 5 (UBR5) ∆UBR (UBR5 Human)
    

    Use

    Cloning

    Mutation

    W1235L (bp g3704t)

    Promoter

    N/A

    Available Since

    Jan. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. Nxph4-2A-CreERT2 Targeting Vector

    Plasmid

    #62219

    Purpose

    Targets CreERT2 to the mouse Nxph4 stop codon

    Depositor

    Hongkui Zeng

    Article

    Harris et al Front Neural Circuits. 2014 Jul 10;8:76.

    Insert

    Nxph4-2A-CreERT2 (Nxph4 Mouse, Bacteriophage)
    

    Use

    Cre/Lox and Mouse Targeting

    Expression

    Mammalian

    Promoter

    Mouse Nxph4

    Available Since

    June 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pENTR221-EF1.Pro

    Plasmid

    #79488

    Purpose

    MultiSite Gateway entry clones, first fragment, (attL1, attR5) Human EF1 promoter

    Depositor

    Peter Jon Nelson

    Article

    Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

    Insert

    EF1 Promoter (EEF1A1 Synthetic, Human)
    

    Use

    Multisite gateway entry clone

    Promoter

    EF1 Promoter

    Available Since

    May 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pENTR221 UBR5 ∆HECT

    Plasmid

    #81065

    Purpose

    Gateway entry vector encoding full length UBR5 C2768A (∆HECT)

    Depositor

    Darren Saunders

    Article

    Shearer et al Mol Biol Cell. 2018 May 9:mbcE17040248.

    Insert

    Ubiquitin protein ligase E3 component N-recognin 5 (UBR5) ∆HECT (UBR5 Human)
    

    Use

    Cloning

    Mutation

    C2768A (bp t8302g and g8303c)

    Promoter

    N/A

    Available Since

    Jan. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. MBP-FUS_FL_6E

    Plasmid

    #98655

    Purpose

    Expresses MBP-tagged full length phosphomimetic FUS

    Depositor

    Nicolas Fawzi

    Article

    Monahan et al EMBO J. 2017 Aug 8. pii: e201696394. doi

    Insert

    FUS (FUS Human)
    

    Tags

    6xHis-MBP

    Expression

    Bacterial

    Mutation

    6 S/T to E

    Available Since

    Aug. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pFB-GST-IRP2

    Plasmid

    #226621

    Purpose

    Expresses human IRP2 in insect cells

    Depositor

    Ning Zheng

    Article

    Wang et al Mol Cell. 2020 Apr 2;78(1):31-41.e5. doi

    Insert

    IRP2 (IREB2 Human)
    

    Tags

    GST

    Expression

    Insect

    Promoter

    Polyhedrin

    Available Since

    Oct. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHRIG-AktDN

    Plasmid

    #53597

    Purpose

    lentiviral expression of dominant negative mouse Akt1 and EGFP

    Depositor

    Heng Zhao

    Article

    Xie et al J Cereb Blood Flow Metab. 2013 Dec;33(12

    Insert

    Akt1 (Akt1 Mouse)
    

    Use

    Lentiviral

    Tags

    HA and IRES-EGFP

    Expression

    Mammalian

    Mutation

    Dominant negative (K179M)

    Promoter

    CMV

    Available Since

    Aug. 25, 2014

    Availability

    Academic Institutions and Nonprofits only

19. pET-A3A(N57Q)-BE3

    Plasmid

    #158157

    Purpose

    T7 promoter bacterial expression plasmid for hAPOBEC3A(N57Q)-BE3 with N-terminal 6xHis-tag and C-terminal SV40 NLS

    Depositor

    Keith Joung

    Article

    Zeng et al Nat Med. 2020 Apr;26(4):535-541. doi: 10

    Insert

    6xHis-hA3A(N57Q)-XTEN linker-hSpCas9n(D10A)-UGI-SV40NLS
    

    Tags

    6xHis tag on N-terminus

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLV Cbx3-V5

    Plasmid

    #175104

    Purpose

    Lentiviral expression of mouse Cbx3-V5

    Depositor

    Larry Gerace

    Article

    Cheng et al J Proteome Res. 2022 Sep 2;21(9):2197-22

    Insert

    Cbx3 (Cbx3 Mouse)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Available Since

    Oct. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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