Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

We narrowed to 157,779 results for: ins

Clear keywords
Showing: 5981 - 6000 of 157779 results

  4. pFastBac HT JS-Munc18c

    Plasmid
    #135555
    Purpose
    Express mouse Munc18c in Sf9 cells, the resulted plasmid encodes an N-terminally His6-tagged Munc18c protein with a tobacco etch virus (TEV) cleavage site between the His6 tag and Munc18c.
    Depositor
    Jingshi Shen
    Article
    Yu et al Methods Mol Biol. 2019;1860:237-249. doi
    Insert
    Munc18c
    Tags
    6xHis tag and TEV site
    Expression
    Insect
    Promoter
    polyhedrin promoter
    Available Since
    Feb. 20, 2020
    Availability
    Academic Institutions and Nonprofits only
    135555-plasmid-map-sequence-id-272498

    Enlarge

  11. MSP1673

    Plasmid
    #65769
    Purpose
    Bacterial expression plasmid for S. thermophilus1 Cas9 & sgRNA (need to clone in spacer into BspMI sites): T7-humanSt1Cas9-NLS-T7-BspMIcassette-St1-sgRNA
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature
    Insert
    mammalian codon-optimized Streptococcus thermophilus1 Cas9-NLS, and St1Cas9 gRNA
    Use
    CRISPR
    Tags
    NLS
    Expression
    Bacterial
    Promoter
    T7 (x2)
    Available Since
    June 24, 2015
    Availability
    Academic Institutions and Nonprofits only
    65769-plasmid-map-sequence-id-112659

    Enlarge

  13. BPK2101

    Plasmid
    #65770
    Purpose
    Bacterial expression plasmid for S. aureus Cas9 & sgRNA (need to clone in spacer into BsaI sites): T7-humanSaCas9-NLS-3xFLAG-T7-BsaIcassette-Sa-sgRNA
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature
    Insert
    mammalian codon-optimized SaCas9, and SaCas9 gRNA
    Use
    CRISPR
    Tags
    NLS-3xFlag
    Expression
    Bacterial
    Promoter
    T7 (x2)
    Available Since
    June 24, 2015
    Availability
    Academic Institutions and Nonprofits only
    65770-plasmid-map-sequence-id-112650

    Enlarge

  16. pET28a Gx-mNG-NL

    Plasmid
    #169106
    Purpose
    Expresses protein G fused to mNeonGreen and NanoLuc. The protein G domain contains an amber stop-codon at position 24 for incorporation of the unnatural amino acid para-benzoylphenylalanine.
    Depositor
    Maarten Merkx
    Article
    Wouters et al Bioconjug Chem. 2020 Mar 18;31(3):656-66
    Insert
    protein G - mNeonGreen - NanoLuc
    Tags
    his-tag and strep-tag
    Expression
    Bacterial
    Mutation
    Ala 24 stop
    Promoter
    T7
    Available Since
    June 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    169106-plasmid-map-sequence-id-335361

    Enlarge

  17. f-CTS-EGFP

    Plasmid
    #86064
    Purpose
    A mammalian expression plasmid encoding fibrocystin ciliary targeting signal domain with C-terminus EGFP.
    Depositor
    Lei Lu
    Article
    Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393
    Insert
    Fibrocystin (Pkhd1 Mouse)
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    contains ciliary targeting signal (3870-3887 amin…
    Promoter
    CMV
    Available Since
    May 8, 2017
    Availability
    Academic Institutions and Nonprofits only
    86064-plasmid-map-sequence-id-179754

    Enlarge

Showing: 5981 - 6000 of 157779 results