We narrowed to 150,751 results for: ins

1. pCIneo-PAM-sig-His6-gamma-PALcc-deltaGlyc

   Plasmid

   #25458

   Depositor

   Betty Eipper

   Article

   Chufan et al Structure. 2009 Jul 15. 17(7):965-73.

   Insert

   PAM (Pam Rat)
   

   Tags

   His6 and PAM signal

   Expression

   Mammalian

   Mutation

   Contains aa 498-820 (PALcc= PAL catalytic core) …

   Available Since

   July 13, 2010

   Availability

   Academic Institutions and Nonprofits only

2. Vh-HA-2xFKBP

   Plasmid

   #20221

   Depositor

   Carolyn Bertozzi

   Article

   Czlapinski et al J Am Chem Soc. 2008 Oct 8. 130(40):13186

   Insert

   2xFKBP (FKBP1A Human)
   

   Tags

   HA and Vh

   Expression

   Mammalian

   Available Since

   Feb. 27, 2009

   Availability

   Academic Institutions and Nonprofits only

3. pSCM167-Venus-VF-hADAM10(aa455-654)

   Plasmid

   #189561

   Purpose

   Protein expression ADAM10 in mammalian cells. Contains only disintegrin and cysteine rich domains--amino acids 455-654

   Depositor

   Haian Fu

   Insert

   truncated ADAM metallopeptidase domain 10 (ADAM10 Human)
   

   Tags

   VF

   Expression

   Mammalian

   Mutation

   contains aa 455-654

   Available Since

   Oct. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. PKG I beta CAT

   Plasmid

   #16396

   Depositor

   Bernard Weinstein

   Article

   Deguchi et al Mol Cancer Ther. 2002 Aug . 1(10):803-9.

   Insert

   PKG1 beta (PRKG1 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   CAT: catalytic domain

   Available Since

   April 14, 2009

   Availability

   Academic Institutions and Nonprofits only

5. pMTH PKC beta II

   Plasmid

   #8410

   Depositor

   Frederic Mushinski

   Article

   Mischak et al J Biol Chem 1993 Sep 25;268(27):20110-5.

   Insert

   Rat Protein Kinase C beta II (Prkcb Rat)
   

   Expression

   Mammalian

   Available Since

   April 28, 2006

   Availability

   Academic Institutions and Nonprofits only

6. pIC389

   Plasmid

   #37387

   Depositor

   Iain Cheeseman

   Article

   Kiyomitsu et al Nat Cell Biol. 2012 Feb 12;14(3):311-7.

   Insert

   LGN (GPSM2 Human)
   

   Use

   Retroviral

   Tags

   GFP, S-tag, and TEV

   Expression

   Mammalian

   Mutation

   deleted amino acid 1-364 and 524

   Available Since

   July 19, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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7. Hy_pMT Laccase2 MCS-WT Split GFP

   Plasmid

   #69891

   Purpose

   Expresses a GFP circular RNA in Drosophila

   Depositor

   Jeremy Wilusz

   Article

   Kramer et al Genes Dev. 2015 Oct 8.

   Insert

   GFP, laccase2 (stw Fly)
   

   Expression

   Insect

   Promoter

   Metallothionein Promoter (pMT)

   Available Since

   Nov. 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-CaMKIIa-ChRger3-TS-EYFP

   Plasmid

   #127241

   Purpose

   High photocurrent, low-light sensitive channelrhodopsin (ChRger3) for optogenetic activation with systemic delivery or for low-light activation. Driven by the CamKIIa promoter.

   Depositor

   Viviana Gradinaru

   Article

   Bedbrook et al Nat Methods. 2019 Oct 14. pii: 10.1038/s

   Insert

   ChRger3-TS-EYFP
   

   Use

   AAV

   Tags

   EYFP and SpyTag

   Expression

   Mammalian

   Promoter

   CamKIIa

   Available Since

   Sept. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1 3xFLAG TPP1 88-249

   Plasmid

   #53549

   Purpose

   mammalian expression of TPP1 OB-fold domain

   Depositor

   Kathleen Collins

   Article

   Sexton et al J Biol Chem. 2012 Oct 5;287(41):34455-64

   Insert

   TPP1 (ACD Human)
   

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   aa 88-249

   Promoter

   CMV

   Available Since

   May 29, 2014

   Availability

   Academic Institutions and Nonprofits only

10. pBabe-Tet-off-puro-mCherry-PA(wt)-Vav2

    Plasmid

    #91873

    Purpose

    Photo-activatable/light sensitive Vav2

    Depositor

    Klaus Hahn

    Article

    Dagliyan et al Science. 2016 Dec 16;354(6318):1441-1444

    Inserts

    LOV2
    VAV2 DH/PH/ZF (Vav2 Mouse)
    

    Tags

    mCherry

    Expression

    Bacterial and Mammalian

    Available Since

    July 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGEX Stat4-SH2

    Plasmid

    #46514

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    Stat4 (STAT4 Human)
    

    Tags

    GST and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 133-678

    Promoter

    Tac

    Available Since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGEX Syk(C)-SH2

    Plasmid

    #46519

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    Syk(C) (SYK Human)
    

    Tags

    GST and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 149-263

    Promoter

    Tac

    Available Since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET28a Gx-NL2

    Plasmid

    #169107

    Purpose

    Expresses protein G fused to 2 copies of NanoLuc. The protein G domain contains an amber stop-codon at position 24 for incorporation of the unnatural amino acid para-benzoylphenylalanine.

    Depositor

    Maarten Merkx

    Article

    Wouters et al Bioconjug Chem. 2020 Mar 18;31(3):656-66

    Insert

    protein G - NanoLuc - NanoLuc
    

    Tags

    his-tag and strep-tag

    Expression

    Bacterial

    Mutation

    Ala 24 stop

    Promoter

    T7

    Available Since

    June 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGEX Slap-SH2

    Plasmid

    #46500

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    Slap (SLA Human)
    

    Tags

    GST and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 61-221

    Promoter

    Tac

    Available Since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBABE bleo mouse IRS-2

    Plasmid

    #11447

    Depositor

    Ronald Kahn

    Article

    Tsuruzoe et al Mol Cell Biol. 2001 Jan . 21(1):26-38.

    Insert

    IRS-2 (Irs2 Mouse)
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    May 25, 2006

    Availability

    Academic Institutions and Nonprofits only

16. pGEX Vav1-SH2

    Plasmid

    #46528

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    Vav1 (VAV1 Human)
    

    Tags

    AviTag, GST, and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 661-777

    Promoter

    Tac

    Available Since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. B. fragilis mRNA toehold switch sensor

    Plasmid

    #110706

    Purpose

    Toehold switch sensor to detect a species specific mRNA with GFP output

    Depositor

    James Collins

    Article

    Takahashi et al Nat Commun. 2018 Aug 21;9(1):3347. doi:

    Insert

    B. fragilis species specific toehold switch sensor
    

    Use

    Synthetic Biology

    Promoter

    T7

    Available Since

    Sept. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. 2Bc-T MBP_BoMoC(ed)_I770A_6xH

    Plasmid

    #185711

    Purpose

    Recombinant B. mori truncated R2 RT expression

    Depositor

    Kathleen Collins

    Article

    Pimentel et al J Biol Chem. 2022 Mar;298(3):101624. doi

    Insert

    B. mori truncated R2 RT
    

    Tags

    6xHis and MBP

    Expression

    Bacterial

    Mutation

    Changed I770 to A

    Promoter

    T7

    Available Since

    Aug. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCarCBA

    Plasmid

    #117754

    Purpose

    Codon optimized CarCBA enzymes from Pectobacterium carotovorum in pBbaA5k backbone

    Depositor

    Greg Bokinsky

    Article

    Shomar et al Nat Chem Biol. 2018 Aug;14(8):794-800. d

    Insert

    Codon optimised Carbapenem synthase (CarC), Carboxymethylproline synthase (CarB), Carbapenam synthetase (CarA)
    

    Expression

    Bacterial

    Promoter

    PLac(UV5)

    Available Since

    Nov. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. GFP-CmTMC1truncated

    Plasmid

    #154908

    Purpose

    For the expression and purification of the truncated TMC1 protein from Chelonia mydas. The protease cleavage site was replaced with the HRV 3C site.

    Depositor

    Motoyuki Hattori

    Article

    Jia et al Neuron. 2020 Jan 22;105(2):310-321.e3. d

    Insert

    GFP-CmTMC1truncated
    

    Tags

    8histidine-EGFP

    Expression

    Insect

    Mutation

    Truncations of the residues 1-243 and 843-969

    Available Since

    Sept. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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