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pRSET FLII12Pglu-700uDelta6

Plasmid

#13568

Depositor

Wolf Frommer

Article

Takanaga et al Biochim Biophys Acta. 2008 Apr . 1778(4)

Insert

FLIPglu F16A Y12S-ECFP-D13R, 6aa linker

Tags

Citrine

Expression

Bacterial

Mutation

F16A, ECFP insert in mglB

Available Since

Feb. 7, 2008

Availability

Academic Institutions and Nonprofits only

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pGEX-GST-AGO1

Plasmid

#24316

Depositor

Carl Novina

Article

Wang et al Nat Struct Mol Biol. 2009 Dec . 16(12):1

Insert

human AGO1 (AGO1 Human)

Tags

GST

Expression

Bacterial

Available Since

April 7, 2010

Availability

Academic Institutions and Nonprofits only
pRN3P_AID-Dam-only

Plasmid

#136065

Purpose

in vitro transcription for mRNA

Depositor

Maria-Elena Torres-Padilla

Article

Borsos et al Nature. 2019 May;569(7758):729-733. doi:

Insert

AID-Dam only

Use

In vitro transcription

Available Since

March 26, 2020

Availability

Academic Institutions and Nonprofits only

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pmVenus-C2-MmMKL2

Plasmid

#67894

Purpose

Expresses a fusion of mVenus with MKL2 (MRTF-B) in mammalian cells

Depositor

Dorus Gadella

Article

van Unen et al Sci Rep. 2015 Oct 5;5:14693. doi: 10.103

Insert

mVenus-MKL2

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 12, 2016

Availability

Academic Institutions and Nonprofits only

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Mef2A-shRNA

Plasmid

#32899

Depositor

Michael Greenberg

Article

Flavell et al Science. 2006 Feb 17;311(5763):1008-12.

Insert

Mef2a (Mef2a Rat)

Use

RNAi

Expression

Mammalian

Promoter

H1-RNA

Available Since

Nov. 18, 2011

Availability

Academic Institutions and Nonprofits only
pLentiCRISPR V2 sgIREB2_1

Plasmid

#169894

Purpose

Disrupt IREB2

Depositor

Richard Possemato

Article

Terzi et al Sci Adv. 2021 May 26;7(22). pii: 7/22/ea

Insert

sgRNA targeting IREB2

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

May 28, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP-Bik

Plasmid

#10952

Depositor

Bingliang Fang

Article

Kagawa et al Cancer Res. 2001 Apr 15. 61(8):3330-8.

Insert

human Bik gene (BIK Human)

Tags

GFP

Expression

Mammalian

Mutation

NA

Available Since

April 4, 2006

Availability

Academic Institutions and Nonprofits only

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HA-DN-14-3-3 zeta (R56A/R60A)

Plasmid

#116889

Purpose

Expresses HA-tagged human dominant-negative 14-3-3 zeta (R56A/R60A) in mammalian cells

Depositor

FENG-QIAN Li, Ken-Ichi Takemaru

Article

Li et al J Cell Biol. 2008 Jun 30;181(7):1141-54.

Insert

DN-14-3-3 zeta (R56A/R60A) (YWHAZ Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pYX233

Plasmid

#163762

Purpose

Empty vector for Gal-inducible yeast expression

Depositor

Johannes Herrmann

Article

Boos et al Nat Cell Biol. 2019 Apr;21(4):442-451. d

Type

Empty backbone

Tags

Hemagglutinin (HA) tag

Expression

Yeast

Promoter

GAL1

Available Since

Feb. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pDSAR-Cre-F2A-I-SceI

Plasmid

#62302

Purpose

Anopheles docking site transgenesis plasmid expressing Cre recombinase and I-SceI nuclease, vasa promoter, DsRed marker

Depositor

Eric Marois

Article

Volohonsky et al G3 (Bethesda). 2015 Apr 13. pii: g3.115.

Insert

Cre recombinase, I-SceI nuclease

Expression

Insect

Promoter

vasa

Available Since

May 19, 2015

Availability

Academic Institutions and Nonprofits only

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H6hSDHA

Plasmid

#113045

Purpose

Hetero expression of His-tagged human SDHA

Depositor

Edward A. Berry

Article

Zafreen et al Arch Biochem Biophys. 2016 Aug 15;604:47

Insert

H6hSDHA (SDHA Human)

Tags

His6 (MGSSHHHHHHSQDP-)

Expression

Bacterial

Mutation

Insert begins at amino acid 43

Available Since

Aug. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-hSynapsin1-GCaMP6s-P2A-mRuby3

Plasmid

#112007

Purpose

For co-expression of GCaMP6s with mRuby3

Depositor

Lin Tian

Article

Broussard et al Nat Neurosci. 2018 Sep;21(9):1272-1280.

Has Service

AAV9

Insert

GCaMP6s-P2A-mRuby3

Use

AAV

Expression

Mammalian

Promoter

hSynapsin1

Available Since

June 26, 2018

Availability

Academic Institutions and Nonprofits only

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pCAG-p65AD-GBP6

Plasmid

#49440

Purpose

Expression of a GFP-dependent transcription factor component in mammalian cells

Depositor

Connie Cepko

Article

Tang et al Cell. 2013 Aug 15;154(4):928-39. doi: 10

Insert

GBP6-p65 transactivation domain fusion protein

Expression

Mammalian

Promoter

CAG

Available Since

Jan. 9, 2014

Availability

Academic Institutions and Nonprofits only

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pCMV-Tag2B Flag BimEL

Plasmid

#23090

Depositor

Roger Davis

Article

Hubner et al Mol Cell. 2008 May 23. 30(4):415-25.

Insert

Bim EL (Bcl2l11 Mouse)

Tags

Flag

Expression

Mammalian

Available Since

Feb. 26, 2010

Availability

Academic Institutions and Nonprofits only
MARCKS(1-40)-paGFP

Plasmid

#18695

Depositor

Karel Svoboda

Article

Harvey et al Science. 2008 Jul 4. 321(5885):136-40.

Insert

MARCKS(1-40) (MARCKS Human)

Tags

photoactivatable GFP

Expression

Mammalian

Mutation

A206K mutation in paGFP

Available Since

July 2, 2008

Availability

Academic Institutions and Nonprofits only

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ECFP(W66A)-FRB-MoA

Plasmid

#67904

Purpose

Expresses a Mitochondria located FRB domain in mammalian cells (the ECFP is not fluorescent)

Depositor

Dorus Gadella

Article

van Unen et al Sci Rep. 2015 Oct 5;5:14693. doi: 10.103

Insert

ECFP(W66A)-FRB-MoA

Use

Synthetic Biology

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 12, 2016

Availability

Academic Institutions and Nonprofits only

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pCAG-iCre

Plasmid

#125577

Purpose

Expresses iCre under the CAG promoter in mammalian cells.

Depositor

Takeshi Imai

Article

Fujimoto et al Dev Cell. 2023 Jul 24;58(14):1221-1236.e

Insert

iCre

Expression

Mammalian

Available Since

July 8, 2019

Availability

Academic Institutions and Nonprofits only

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pLJM60-S6K1(wt) barcoded

Plasmid

#48801

Purpose

expresses wt S6K1

Depositor

David Sabatini

Article

Kang et al Science. 2013 Jul 26;341(6144):1236566.

Insert

S6K1 (Rps6kb1 Rat)

Use

Lentiviral

Tags

FLAG and barcode: GGATCC

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 18, 2013

Availability

Academic Institutions and Nonprofits only
pCR3-vsv-Sgo1 N61I

Plasmid

#108496

Purpose

expression of vsv-Sgo1 N61I (no PP2A binding)

Depositor

Susanne Lens

Article

Meppelink et al Cell Rep. 2015 Apr 28;11(4):508-15. doi:

Insert

Shugoshin 1 (SGO1 Human)

Tags

VSV

Expression

Mammalian

Mutation

no PP2A binding N61I

Promoter

CMV

Available Since

May 8, 2018

Availability

Academic Institutions and Nonprofits only

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pCW-LIC-N8C_Fab4a

Plasmid

#203690

Purpose

Expresses N8C_Fab4a

Depositor

Brenda Schulman

Article

Henneberg et al Nat Chem Biol. 2023 Dec;19(12):1513-1523

Insert

N8C_Fab4a

Expression

Bacterial

Available Since

Aug. 31, 2023

Availability

Academic Institutions and Nonprofits only

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