We narrowed to 13,863 results for: mpl

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SDHC-pHLuorin-sems

Plasmid

#107767

Purpose

labeling of mitochondrial succinate dehydrogenase subunit C, pH measurements

Depositor

Karin Busch

Article

Rieger et al Sci Rep. 2017 Apr 6;7:46055. doi: 10.103

Insert

Subunit C of SDH fused to pHluorin (SDHC Human)

Tags

pHluorin

Expression

Mammalian

Promoter

CMV

Available Since

April 20, 2018

Availability

Academic Institutions and Nonprofits only

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EGFP-Caveolin-3

Plasmid

#68396

Purpose

Fluorescently tagged Caveolin-3 for expression in mammalian cells

Depositor

Rob Parton

Article

Luetterforst et al J Cell Biol. 1999 Jun 28;145(7):1443-59.

Insert

Cav3 (Cav3 Mouse)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 20, 2015

Availability

Academic Institutions and Nonprofits only
pAAV-CaMKII-jGCaMP7f-WPRE

Plasmid

#203848

Purpose

Expression of GCaMP7f in CaMKII expressing cells

Depositor

Ivo Spiegel

Article

Cohen-Kashi Malina et al Neuron. 2021 Jul 7;109(13):2150-2164.e5.

Insert

jGCaMP7f

Use

AAV

Available Since

Aug. 9, 2023

Availability

Academic Institutions and Nonprofits only

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Halotag-6xHp

Plasmid

#214414

Purpose

Lipid droplet localization of 6xHp of Spastin fused to Halotag

Depositor

Chi-Lun Chang

Article

Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

Insert

6x Spastin aa 43-92 (SPAST Human)

Tags

Halo

Expression

Mammalian

Promoter

CMV

Available Since

March 18, 2024

Availability

Academic Institutions and Nonprofits only

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pU6-sgRNA-CBh-eGFP

Plasmid

#206885

Purpose

cloning backbone for sgRNA using OptScf2

Depositor

Jacob Giehm Mikkelsen

Article

Haldrup et al Nucleic Acids Res. 2023 Sep 8:gkad676. d

Type

Empty backbone

Promoter

U6

Available Since

Oct. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti-PGK-Hygro-amp-sgRNA-resistant PBRM1

Plasmid

#107407

Purpose

Lentiviral expression of gRNA resistant PBRM1

Depositor

William Kaelin

Article

Gao et al Proc Natl Acad Sci U S A. 2017 Jan 31;11

Insert

sg resistant PBRM1 (PBRM1 Human)

Use

Lentiviral

Mutation

sgRNA resistant

Available Since

May 22, 2018

Availability

Academic Institutions and Nonprofits only

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pJFRC-13XLexAoP-FRT-STOP-FRT-myrGFP-2A-KDR::Pest

Plasmid

#217515

Purpose

Encodes a LexAoP controlled and Flp dependent conditional trangene of bicistronic myrGFP and KD Recombinase

Depositor

S. Lawrence Zipursky

Article

Sanfilippo et al Neuron. 2024 Jan 13:S0896-6273(23)00973-

Insert

13XLexAoP-FRT-STOP-FRT-myrGFP-2A-KDR::Pest

Expression

Insect

Available Since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

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pMBEC2

Plasmid

#187596

Purpose

Base editing plasmid, constitutively expressing cytosine base editor and cas6. Contains GFP, flanked by BsaI restriction sites to introduce spacer and gRNAs. Kanamycin resistance

Depositor

Pablo Ivan Nikel

Article

Volke et al Nat Commun. 2022 May 31;13(1):3026. doi:

Insert

nCas9-BEC-UGI; cas6f, gfp

Expression

Bacterial

Promoter

trc

Available Since

July 27, 2022

Availability

Academic Institutions and Nonprofits only

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TERTpWT_Gluc_CCR5

Plasmid

#192270

Purpose

Donor template for insertion of hTERT promoter driven Gaussia luciferase reporter

Depositor

Shantanu Chowdhury

Article

Sharma et al Cell Rep. 2021 May 18;35(7):109154. doi:

Insert

Promoter Reporter Insert within donor template

Use

CRISPR

Promoter

TERT promoter driving expression of gaussia

Available Since

Dec. 20, 2022

Availability

Academic Institutions and Nonprofits only

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keratin 8 (krt8)-H2B-RFP

Plasmid

#105955

Purpose

transgenesis

Depositor

Caren Norden

Article

Dzafic et al Cell Rep. 2015 Oct 6;13(1):168-182. doi:

Insert

krt8 (KRT8 Human)

Tags

RFP

Expression

Bacterial

Available Since

Feb. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pMOD_D-AtUBI10-NbGP41-sfGFP-VP64-GB1

Plasmid

#202012

Purpose

Moclo level 1 - Module D, Promoter: AtUBI10, Gene: NbGP41P-sfGFP-VP64-GB1, Terminator: pea rbcsE9

Depositor

Michael Smanski

Article

Casas-Mollano et al Nucleic Acids Res. 2023 Jul 21;51(13):70

Insert

NbGP41-sfGFP-VP64-GB1

Use

Synthetic Biology; Moclo level 1 vector

Tags

GB1 and sfGFP

Promoter

AtUBI10

Available Since

July 31, 2023

Availability

Academic Institutions and Nonprofits only

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pMDJ113 - pEXP(HygroR, 5605)[Phsp-16.41 |Cre recombinase (PATCs) | tbb-2 UTR]

Plasmid

#159809

Purpose

Optimized enzyme for genome editing in C. elegans

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

pEXP(HygroR, 5605)[Phsp-16.41 |Cre recombinase (PATCs) | tbb-2 UTR]

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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5' Del 1

Plasmid

#16049

Depositor

Robert Benezra

Article

Tournay et al Mol Cell Biol. 1996 May . 16(5):2418-30.

Insert

Id1 promoter, 5' deletion (Id1 Mouse)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Mutation

-1372 to +88 of promoter

Available Since

Nov. 2, 2007

Availability

Academic Institutions and Nonprofits only
5' Del 2

Plasmid

#16050

Depositor

Robert Benezra

Article

Tournay et al Mol Cell Biol. 1996 May . 16(5):2418-30.

Insert

Id1 promoter, 5' deletion (Id1 Mouse)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Mutation

-1147 to +88 of promoter

Available Since

Nov. 2, 2007

Availability

Academic Institutions and Nonprofits only
pTAMAHISTEV_PfeA

Plasmid

#128943

Purpose

expresses PfeA in E. coli

Depositor

James Naismith

Article

Moynie et al Nat Commun. 2019 Aug 14;10(1):3673. doi:

Insert

ferric enterobactin receptor

Tags

TEV protease cleavable 7xHis and TamA

Expression

Bacterial

Mutation

Signal peptide sequence has been removed (1-75)

Promoter

T7

Available Since

Aug. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pLYS1-MICU1-Flag

Plasmid

#50058

Purpose

Expression of human MICU1 with Flag tag in mammalian cells

Depositor

Vamsi Mootha

Article

Sancak et al Science. 2013 Nov 14.

Insert

MICU1 (isoform 2) (MICU1 Human)

Tags

Flag

Expression

Mammalian

Mutation

*

Available Since

May 30, 2014

Availability

Academic Institutions and Nonprofits only

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pTF023_His_hsDDA1

Plasmid

#174480

Purpose

Expresses DDA1 in insect cells

Depositor

Eric Fischer

Article

Faust et al Nat Chem Biol. 2020 Jan;16(1):7-14. doi:

Insert

DDA1 (DDA1 Human)

Tags

His-TEV

Expression

Insect

Available Since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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bArr1-V5

Plasmid

#201492

Purpose

Expression of the beta-arrestin1 containing a C-terminal V5 tag.

Depositor

Patrick Giguère

Article

Zeghal et al J Biol Chem. 2023 Jul 28:105107. doi: 10

Insert

bArr1-V5 (ARRB1 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

EF1a

Available Since

Aug. 10, 2023

Availability

Academic Institutions and Nonprofits only

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sgPBRM1-1- LentiCRISPRv2

Plasmid

#107403

Purpose

Lentiviral expression of Cas9 and gRNA targeting PBRM1

Depositor

William Kaelin

Article

Gao et al Proc Natl Acad Sci U S A. 2017 Jan 31;11

Insert

gRNA targeting PBRM1 (PBRM1 Human)

Use

Lentiviral

Available Since

June 14, 2018

Availability

Academic Institutions and Nonprofits only

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pdRBFC-B2-YN

Plasmid

#74721

Purpose

Express C-terminal YN fused flock house virus (FHV) B2 protein (2741-2956 nt) for dsRNA labeling

Depositor

Aiming Wang

Article

Cheng et al Virology. 2015 Nov;485:439-51. doi: 10.1

Insert

Influenza A virus NS1 protein, partial

Use

Double-stranded rna labeling

Tags

HA and YFP N-terminal half domain

Expression

Plant

Promoter

CaMV 35S

Available Since

May 9, 2016

Availability

Academic Institutions and Nonprofits only

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