We narrowed to 23,847 results for: LEA

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VP16-ER beta (long)

Plasmid

#11352

Depositor

Donald McDonnell

Article

Chang et al Mol Cell Biol. 1999 Dec . 19(12):8226-39

Insert

ER beta long (ESR2 Human)

Expression

Mammalian

Available Since

May 25, 2006

Availability

Academic Institutions and Nonprofits only

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GFP-DroshaS300/302A

Plasmid

#62530

Purpose

Serines 300 and 302 of Drosha are mutated to alanines

Depositor

Bharat Ramratnam

Article

Tang et al Nucleic Acids Res. 2010 Oct;38(19):6610-

Insert

human Drosha with serines 300 and 302 changed alanines (DROSHA Human)

Tags

GFP

Expression

Mammalian

Mutation

changed serines 300 and 302 to alanines

Available Since

March 20, 2015

Availability

Academic Institutions and Nonprofits only
LCV2 V-1

Plasmid

#84804

Purpose

Cas9 with sgRNA targeting Exon 1 of VEGF-A

Depositor

Glenn Yiu

Article

Yiu et al Invest Ophthalmol Vis Sci. 2016 Oct 1;57

Insert

gRNA targeting VEGF-A (VEGFA Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Nov. 8, 2016

Availability

Academic Institutions and Nonprofits only

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proISG15 (2-165)

Plasmid

#110762

Purpose

Bacterial expression of proISG15

Depositor

David Komander

Article

Swatek et al Proc Natl Acad Sci U S A. 2018 Mar 6;115

Insert

ISG15 (ISG15 Human)

Tags

N-His6-3C

Expression

Bacterial

Promoter

T7

Available Since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

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pLVH-EF1a-GFP-P2A-KLF4

Plasmid

#130694

Purpose

Lentiviral plasmids encoding human KLF4 with co-expressoin of GFP mediated by P2A.

Depositor

Kejin Hu

Article

Shao et al Nat Commun. 2016 Mar 7;7:10869. doi: 10.

Insert

human KLF4 (KLF4 Human)

Use

Lentiviral

Available Since

Aug. 22, 2019

Availability

Academic Institutions and Nonprofits only

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LCV2 V-3

Plasmid

#84806

Purpose

Cas9 with sgRNA targeting Exon 1 of VEGF-A

Depositor

Glenn Yiu

Article

Yiu et al Invest Ophthalmol Vis Sci. 2016 Oct 1;57

Insert

gRNA targeting VEGF-A (VEGFA Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Nov. 8, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn Con/Foff EYFP

Plasmid

#55651

Purpose

Cre-on/Flp-off EYFP under the Synapsin promoter

Depositor

Karl Deisseroth

Article

Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

Insert

eYFP with introns

Use

AAV; Cre on/flp off eyfp

Expression

Mammalian

Promoter

hSyn

Available Since

Aug. 11, 2014

Availability

Academic Institutions and Nonprofits only

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pYFP-ErbB2

Plasmid

#66948

Purpose

Mammalian expression of rat ErbB2 tagged with YFP

Depositor

Mads Lerdrup, Bo van_Deurs

Article

Lerdrup et al Mol Biol Cell. 2007 Sep;18(9):3656-66. E

Insert

ErbB2 (Erbb2 Rat)

Tags

6xHis, V5 Tag, and YFP

Expression

Mammalian

Mutation

(See depositor comment below on mutations A24T an…

Promoter

CMV

Available Since

Sept. 15, 2015

Availability

Academic Institutions and Nonprofits only
pLenti-PGK-c-myc-2A-CDK4(R24C)-GFP-Puro

Plasmid

#83107

Purpose

3rd generation lentiviral expression of C-myc, CDK4(R24C), and GFP separated by 2A self-cleaving peptide

Depositor

Andrew Brooks

Inserts

c-Myc (MYC Human)
CDK4 (CDK4 Human)
eGFP

Use

Lentiviral

Tags

2A self cleaving peptide

Expression

Mammalian

Mutation

R24C and p64 isoform

Promoter

hPGK and hPGK (single transcript)

Available Since

July 3, 2017

Availability

Academic Institutions and Nonprofits only

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pGP-CMV-GCaMP6-210

Plasmid

#91778

Purpose

Mammalian expression of low affinity calcium-sensing fluorescent protein

Depositor

Douglas Kim, GENIE Project

Article

de Juan-Sanz et al Neuron. 2017 Jan 30. pii: S0896-6273(17)

Insert

GCaMP6-210

Tags

6xHis, T7 epitope, and Xpress tag

Expression

Mammalian

Promoter

CMV

Available Since

June 27, 2017

Availability

Academic Institutions and Nonprofits only

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OCT4 GFP puro Donor 1

Plasmid

#22209

Depositor

Rudolf Jaenisch

Article

Hockemeyer et al Nat Biotechnol. 2009 Sep;27(9):851-7. do

Insert

SA-OCT-GFP-2APuro-PA (POU5F1 Human)

Available Since

Oct. 5, 2009

Availability

Academic Institutions and Nonprofits only

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SHMT2-RES

Plasmid

#72880

Purpose

overexpression

Depositor

David Sabatini

Article

Kim et al Nature. 2015 Apr 16;520(7547):363-7. doi

Insert

SHMT2 (SHMT2 Human)

Use

Retroviral

Expression

Mammalian

Mutation

silent mutations in site corresponding to shSHMT2…

Available Since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only
pICSL11060

Plasmid

#68264

Purpose

Level 1 Golden Gate Cassette: Cas9 expression cassette for plants (exemplified in Brassica oleracea)

Depositor

Nicola Patron

Article

Lawrenson et al Genome Biol. 2015 Nov 30;16(1):258. doi:

Insert

Promoter/5UTR: CsVMV+ CDS:SpCas9 + 3UTR/terminator:35s

Use

Synthetic Biology

Expression

Plant

Available Since

Aug. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pHR-HP1α-miRFP670-Cry2WT

Plasmid

#122442

Purpose

Expresses disordered protein HP1α fused with fluorescent protein miRFP670 and optogenetic protein Cry2WT

Depositor

Clifford Brangwynne

Article

Shin et al Cell. 2018 Nov 29;175(6):1481-1491.e13.

Insert

HP1 alpha (CBX5 Human)

Use

Lentiviral

Tags

Cry2WT and miRFP670

Expression

Mammalian

Promoter

SFFV

Available Since

March 8, 2019

Availability

Academic Institutions and Nonprofits only

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GFP-BLM DM

Plasmid

#80071

Purpose

Expression of a sumo-mutant form GFP-BLM with K317R and K331R sumo-site mutations

Depositor

Nathan Ellis

Article

Eladad et al Hum Mol Genet. 2005 May 15;14(10):1351-6

Insert

BLM (BLM Human)

Tags

GFP-BLM

Expression

Mammalian

Mutation

K317R (AAA>AGA) and K331R (AAA>AGA)

Available Since

July 25, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-sCreDIO hChR2(H134R)-EYFP

Plasmid

#55642

Purpose

sCre-Dependent ChR2-EYFP

Depositor

Karl Deisseroth

Article

Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

Insert

hChR2(H134R)-EYFP

Use

AAV; Scre-dependent

Tags

EYFP

Expression

Mammalian

Promoter

Ef1a

Available Since

Aug. 8, 2014

Availability

Academic Institutions and Nonprofits only

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pEGFPN1-N-gamma-tubulin (334-449)

Plasmid

#87859

Purpose

Fluorescent fragment of gamma-tubulin containing gamma-tubulin DNA binding domain and NLS

Depositor

Maria Alvarado-Kristensson

Article

Hoog et al FASEB J. 2011 Nov;25(11):3815-27. doi: 1

Insert

gamma-tubulin 1 (TUBG1 Human)

Tags

GFP

Expression

Mammalian

Mutation

fragment from aa 334 to 449

Available Since

May 12, 2017

Availability

Academic Institutions and Nonprofits only

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ID1-pLVXT

Plasmid

#119280

Purpose

Doxycycline-inducible mouse ID1 from 806 cells

Depositor

Joan Massague

Article

Huang et al Cancer Discov. 2019 Oct 3. pii: 2159-829

Insert

Id1 (Id1 Mouse)

Expression

Mammalian

Promoter

TRE

Available Since

Jan. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pMVA2

Plasmid

#119951

Purpose

Expresses recombinant MVA pathway in Escherichia coli

Depositor

Nigel Scrutton

Article

Jervis et al ACS Synth Biol. 2018 Dec 18. doi: 10.102

Inserts

mvaE
mvaS
mvaK1
mvaK2
mvaD
idi

Expression

Bacterial

Mutation

A110G

Available Since

Feb. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA5-PKHD1-myc

Plasmid

#108407

Purpose

Can be used to generate stable Flp-In cells with tetracycline-inducible human polyduction/fibrocystin expression.

Depositor

Gregory Germino

Article

Kaimori et al Hum Mol Genet. 2007 Apr 15. 16(8):942-56

Insert

PKHD1 (PKHD1 Human)

Tags

myc

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 15, 2018

Availability

Academic Institutions and Nonprofits only

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