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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 37,317 results for: kan

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Showing: 6021 - 6040 of 37317 results

  1. G12-CASE

    Plasmid
    #190714
    Purpose
    Encodes a BRET-based activity sensor for the heterotrimeric G protein G12. Composed of the subunits G alpha 12 (GNA12) tagged with NanoLuciferase, G beta 3 (GNB3) and Venus-tagged G gamma 9 (GNG9).
    Depositor
    Gunnar Schulte
    Publication
    Schihada et al J Biol Chem. 2022 Aug 3:102328. doi: 10.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    GNB3 (GNB3 Human)
    GNGT9
    GNA12 (GNA12 Human)
    Use
    Luciferase
    Tags
    Nluc is inserted at A133/F134 within GNA12 and cp…
    Expression
    Mammalian
    Available since
    Dec. 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    190714-plasmid-map-sequence-id-379753

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  2. pG108-K - AhpC-Glow

    Plasmid
    #185135
    Purpose
    Bacteroides - Escherichia coli shuttle vector with TetQ and Kanamycin selection markers BS2 is cloned under the Bacteroides thetaiotaomicron ahpC promoter
    Depositor
    Janina Lewis
    Publication
    Jones et al Mol Oral Microbiol. 2020 Aug;35(4):181-1
    Inserts
    Glow gene
    Bacteroides thetaiotaomicron ahpC promoter
    Use
    Pg106
    Expression
    Bacterial
    Available since
    Dec. 5, 2022
    Availability
    Academic Institutions and Nonprofits only
    185135-plasmid-map-sequence-id-378889

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  3. pMax_mOrange_G299C

    Plasmid
    #177829
    Purpose
    Site-specific mutagenesis of mOrange for the purposes of creating a site-specific G:G mispair for the purposes of measuring MMR activity via Host Cell Reactivation (HCR).
    Depositor
    Zachary Nagel, Leona Samson
    Publication
    Nagel et al Proc Natl Acad Sci U S A. 2014 May 6;111
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mOrange_G299C
    Expression
    Mammalian
    Mutation
    G299C
    Promoter
    CMV
    Available since
    Nov. 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    177829-plasmid-map-sequence-id-348954

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  8. pdCas9-GFP-VPR

    Plasmid
    #181907
    Purpose
    Fluorescently tagged dead Cas9 fused to the tripartite transcriptional activator VP64-p65-Rta
    Depositor
    Karsten Rippe
    Publication
    Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dCas9-VPR
    Use
    CRISPR
    Tags
    GFP
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    April 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    181907-plasmid-map-sequence-id-359690

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  10. pDAS12125_PEAR-GFP

    Plasmid
    #177178
    Purpose
    PEAR-GFP target plasmid with split EGFP and disrupted 5' splice site
    Depositor
    Ervin Welker
    Publication
    Simon et al Elife. 2022 Feb 23;11. pii: 69504. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    EGFP split with an intron between amino acids 95-96
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    disrupted 5' splice site
    Promoter
    CMV
    Available since
    Feb. 3, 2022
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    177178-plasmid-map-sequence-id-348473

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  16. pKm-HCBE

    Plasmid
    #170333
    Purpose
    a modularized single-plasmid cytidine base editing (C-to-T) tool for Sinorhizobium meliloti
    Depositor
    Deqiang Duanmu
    Publication
    Wang et al Front Microbiol. 2021 Jun 18;12:686008.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Rhizobium codon-optimized CBEs
    Use
    CRISPR
    Mutation
    Cas9n-D10A
    Available since
    Aug. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    170333-plasmid-map-sequence-id-341069

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  18. NanoLuc-PM-Venus

    Plasmid
    #164786
    Purpose
    Nanoluciferase (NanoLuc) targeted to the extracellular surface of the cell membrane and intracellularly labeled with monomeric Venus fluorescent protein
    Depositor
    Laszlo Hunyady
    Publication
    Toth et al J Biol Chem. 2021 Feb 2:100366. doi: 10.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Nanoluciferase fused with the transmembrane domain of the platelet-derived growth factor receptor beta and Venus (PDGFRB Oplophorus gracilirostris, Human)
    Tags
    NanoLuc and mVenus
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    May 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    164786-plasmid-map-sequence-id-326679

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  20. pJYDNp

    Plasmid
    #162451
    Purpose
    Positive control plasmid carrying the eUnaG2 bilirubin dependent fluorescent protein fused to the C-terminus of Aga2p protein. This plasmid serves as an expression control.
    Depositor
    Gideon Schreiber
    Publication
    Zahradník et al bioRxiv Preprint 2020
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Aga2p-eUnaG2
    Use
    Shuttle vector bacteria/yeast
    Tags
    HA and myc
    Promoter
    GAL1
    Available since
    Feb. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    162451-plasmid-map-sequence-id-321446

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Showing: 6021 - 6040 of 37317 results