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pJM525 EF1α 3xFLAG-NLS-DsDed-intC-ZF1-NLS-HA in TUPV2

Plasmid

#161554

Purpose

Constitutive expression of 3xFLAG-NLS-DsDed-intC-ZF1-NLS-HA under the EF1α promoter

Depositor

Joshua Leonard

Article

Muldoon et al Sci Adv. 2021 Feb 19;7(8). pii: 7/8/eabe

Insert

3xFLAG-NLS-DsDed-intC-ZF1-NLS-HA

Use

Synthetic Biology

Tags

3x-FLAG and HA

Expression

Mammalian

Promoter

EF1α

Available Since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pJM505 EF1α 3xFLAG-NLS-DsRed-Express2-ZF1 in TUPV2

Plasmid

#161535

Purpose

Constitutive expression of 3xFLAG-NLS-DsRed-Express2-ZF1 under the EF1α promoter

Depositor

Joshua Leonard

Article

Muldoon et al Sci Adv. 2021 Feb 19;7(8). pii: 7/8/eabe

Insert

3xFLAG-NLS-DsRed-Express2-ZF1

Use

Synthetic Biology

Tags

3x-FLAG

Expression

Mammalian

Promoter

EF1α

Available Since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pJM553 ZF10x6-C 3xFLAG-NLS-DsDed-ZF1-PEST in TUPV2

Plasmid

#161540

Purpose

Inducible expression of 3xFLAG-NLS-DsDed-ZF1-PEST under the ZF10x6-C promoter

Depositor

Joshua Leonard

Article

Muldoon et al Sci Adv. 2021 Feb 19;7(8). pii: 7/8/eabe

Insert

3xFLAG-NLS-DsRed-ZF1-PEST

Use

Synthetic Biology

Tags

3x-FLAG

Expression

Mammalian

Promoter

ZF10x6-C

Available Since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pGAL4-DBD-MNX1wt-IDR

Plasmid

#145248

Purpose

Expresses fusion of GAL4 DNA-binding domain and MNX1wt IDR

Depositor

Denes Hnisz

Article

Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

Insert

MNX1wt-IDR (MNX1 Human)

Expression

Mammalian

Available Since

Sept. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pTV-Ctcf-tdiRFP670-PEST

Plasmid

#122357

Purpose

Targeting vector for mouse Ctcf to insert 2A-tdiRFP670-PEST into immediately upstream of stop codon.

Depositor

Hiroshi Ochiai

Article

Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

Insert

2A-tdiRFP-PEST with homology arms for Ctcf

Use

Mouse Targeting

Available Since

June 30, 2020

Availability

Academic Institutions and Nonprofits only

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pTV-Dnmt3l-tdiRFP670-PEST

Plasmid

#122358

Purpose

Targeting vector for mouse Dnmt3l to insert 2A-tdiRFP670-PEST into immediately upstream of stop codon.

Depositor

Hiroshi Ochiai

Article

Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

Insert

2A-tdiRFP-PEST with homology arms for Dnmt3l

Use

Mouse Targeting

Available Since

June 30, 2020

Availability

Academic Institutions and Nonprofits only

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pTV-Etv5-tdiRFP670-PEST

Plasmid

#122359

Purpose

Targeting vector for mouse Etv5 to insert 2A-tdiRFP670-PEST into immediately upstream of stop codon.

Depositor

Hiroshi Ochiai

Article

Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

Insert

2A-tdiRFP-PEST with homology arms for Etv5

Use

Mouse Targeting

Available Since

June 30, 2020

Availability

Academic Institutions and Nonprofits only

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pTV-Jade1-tdiRFP670-PEST

Plasmid

#122360

Purpose

Targeting vector for mouse Jade1 to insert 2A-tdiRFP670-PEST into immediately upstream of stop codon.

Depositor

Hiroshi Ochiai

Article

Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

Insert

2A-tdiRFP-PEST with homology arms for Jade1

Use

Mouse Targeting

Available Since

June 30, 2020

Availability

Academic Institutions and Nonprofits only

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pTV-Kdm1a-tdiRFP670-PEST

Plasmid

#122361

Purpose

Targeting vector for mouse Kdm1a to insert 2A-tdiRFP670-PEST into immediately upstream of stop codon.

Depositor

Hiroshi Ochiai

Article

Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

Insert

2A-tdiRFP-PEST with homology arms for Kdm1a

Use

Mouse Targeting

Available Since

June 30, 2020

Availability

Academic Institutions and Nonprofits only

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pTV-Ncapd3-tdiRFP670-PEST

Plasmid

#122362

Purpose

Targeting vector for mouse Ncapd3 to insert 2A-tdiRFP670-PEST into immediately upstream of stop codon.

Depositor

Hiroshi Ochiai

Article

Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

Insert

2A-tdiRFP-PEST with homology arms for Ncapd3

Use

Mouse Targeting

Available Since

June 30, 2020

Availability

Academic Institutions and Nonprofits only

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pTV-Npm1-tdiRFP670-PEST

Plasmid

#122363

Purpose

Targeting vector for mouse Npm1 to insert 2A-tdiRFP670-PEST into immediately upstream of stop codon.

Depositor

Hiroshi Ochiai

Article

Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

Insert

2A-tdiRFP-PEST with homology arms for Npm1

Use

Mouse Targeting

Available Since

June 30, 2020

Availability

Academic Institutions and Nonprofits only

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pTV-Dnmt3b-tdiRFP670-PEST

Plasmid

#122364

Purpose

Targeting vector for mouse Dnmt3b to insert 2A-tdiRFP670-PEST into immediately upstream of stop codon.

Depositor

Hiroshi Ochiai

Article

Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

Insert

2A-tdiRFP-PEST with homology arms for Dnmt3b

Use

Mouse Targeting

Available Since

June 30, 2020

Availability

Academic Institutions and Nonprofits only

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pTV-Dhx15-tdiRFP670-PEST

Plasmid

#122365

Purpose

Targeting vector for mouse Dhx15 to insert 2A-tdiRFP670-PEST into immediately upstream of stop codon.

Depositor

Hiroshi Ochiai

Article

Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

Insert

2A-tdiRFP-PEST with homology arms for Dhx15

Use

Mouse Targeting

Available Since

June 30, 2020

Availability

Academic Institutions and Nonprofits only

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pTV-Birc6-tdiRFP670-PEST

Plasmid

#122366

Purpose

Targeting vector for mouse Birc6 to insert 2A-tdiRFP670-PEST into immediately upstream of stop codon.

Depositor

Hiroshi Ochiai

Article

Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

Insert

2A-tdiRFP-PEST with homology arms for Birc6

Use

Mouse Targeting

Available Since

June 30, 2020

Availability

Academic Institutions and Nonprofits only

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pTV-Trim28-tdiRFP670-PEST

Plasmid

#122367

Purpose

Targeting vector for mouse Trim28 to insert 2A-tdiRFP670-PEST into immediately upstream of stop codon.

Depositor

Hiroshi Ochiai

Article

Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

Insert

2A-tdiRFP-PEST with homology arms for Trim28

Use

Mouse Targeting

Available Since

June 30, 2020

Availability

Academic Institutions and Nonprofits only

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pTV-Gsk3b-tdiRFP670-PEST

Plasmid

#122368

Purpose

Targeting vector for mouse Gsk3b to insert 2A-tdiRFP670-PEST into immediately upstream of stop codon.

Depositor

Hiroshi Ochiai

Article

Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

Insert

2A-tdiRFP-PEST with homology arms for Gsk3b

Use

Mouse Targeting

Available Since

June 30, 2020

Availability

Academic Institutions and Nonprofits only

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pTV-Cdt1-tdiRFP670-PEST

Plasmid

#122369

Purpose

Targeting vector for mouse Cdt1 to insert 2A-tdiRFP670-PEST into immediately upstream of stop codon.

Depositor

Hiroshi Ochiai

Article

Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

Insert

2A-tdiRFP-PEST with homology arms for Cdt1

Use

Mouse Targeting

Available Since

June 30, 2020

Availability

Academic Institutions and Nonprofits only

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pTV-Ddx42-tdiRFP670-PEST

Plasmid

#122370

Purpose

Targeting vector for mouse Ddx42 to insert 2A-tdiRFP670-PEST into immediately upstream of stop codon.

Depositor

Hiroshi Ochiai

Article

Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

Insert

2A-tdiRFP-PEST with homology arms for Ddx42

Use

Mouse Targeting

Available Since

June 30, 2020

Availability

Academic Institutions and Nonprofits only

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px335-sgRNA-EF(Cas9n)-Ddx42-L

Plasmid

#122343

Purpose

Expresses sgRNA targeting mouse Ddx42 and Cas9 nickase in mammalian cells

Depositor

Hiroshi Ochiai

Article

Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

Insert

sgRNA for mouse Ddx42

Expression

Mammalian

Available Since

June 30, 2020

Availability

Academic Institutions and Nonprofits only

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px335-sgRNA-EF(Cas9n)-Ddx42-R

Plasmid

#122344

Purpose

Expresses sgRNA targeting mouse Ddx42 and Cas9 nickase in mammalian cells

Depositor

Hiroshi Ochiai

Article

Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

Insert

sgRNA for mouse Ddx42

Expression

Mammalian

Available Since

June 30, 2020

Availability

Academic Institutions and Nonprofits only

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