We narrowed to 31,805 results for: LIS;

1. FH-TET3-HxDmut-pEF

   Plasmid

   #127896

   Purpose

   Expresses ten-eleven-translocation 3 (TET3) containing HxD mutation at H1077Y and D1079A

   Depositor

   Patrick Hogan, Anjana Rao

   Article

   Ko et al Nature. 2013 May 2;497(7447):122-6. doi:

   Insert

   TET3-HxDmut (TET3 Human)
   

   Tags

   HA and Flag

   Expression

   Mammalian

   Promoter

   EF-1a

   Available Since

   Oct. 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pENTR223-BGLF4

   Plasmid

   #195811

   Purpose

   Gateway Entry Clone of Epstein-Barr virus BGLF4

   Depositor

   Eric Johannsen

   Article

   Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

   Insert

   BGLF4 (BGLF4 Epstein-Barr virus)
   

   Use

   Gateway entry vector

   Available Since

   May 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. px330-UFL1 sgRNA2

   Plasmid

   #134636

   Purpose

   contains sgRNA targeting human UFL1 for gene knockout

   Depositor

   Yihong Ye

   Article

   Wang et al Cell Res. 2020 Jan;30(1):5-20. doi: 10.1

   Insert

   UFL1 sgRNA2 (UFL1 Human)
   

   Expression

   Mammalian

   Available Since

   Nov. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCDNA3-V5-PTPN14-PPxY

   Plasmid

   #61006

   Purpose

   Expresses human PTPN14 (PPxY domain mutant) in mammalian cells, N-terminus V5 tag

   Depositor

   Jianmin Zhang

   Article

   Wilson et al J Biol Chem. 2014 Aug 22;289(34):23693-7

   Insert

   PTPN14 PPxY mutation (PTPN14 Human)
   

   Tags

   V5 tag

   Expression

   Mammalian

   Mutation

   PPxY mutation

   Promoter

   CMV

   Available Since

   Dec. 4, 2014

   Availability

   Academic Institutions and Nonprofits only

5. VVT1

   Plasmid

   #65779

   Purpose

   The Joung Lab recommends using BPK2660 (Addgene plasmid 70709) instead of VVT1 as guide RNAs cloned into BPK2660 are more effective than this plasmid. Human expression plasmid for S. aureus Cas9 sgRNA

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

   Insert

   SaCas9 gRNA backbone, without spacer sequence
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   June 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. hTRAFD1-pCMV14-3xFlag

   Plasmid

   #73587

   Purpose

   Express human TRAFD1 (FLN29) in mammalian cells

   Depositor

   Paul Odgren

   Article

   Witwicka et al PLoS One. 2015 May 19;10(5):e0127537. do

   Insert

   TRAFD1 (TRAFD1 Human)
   

   Tags

   3xFlag

   Expression

   Mammalian

   Mutation

   The original vector hTRAFD1-mCMV14-Flag was a gif…

   Available Since

   March 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMV-HIS3

   Plasmid

   #65333

   Purpose

   standard plasmids for exogenus pathway assembly haboring yeast auxotrophic marker HIS3 which could be released and ligated to series of TUs

   Depositor

   Junbiao Dai

   Article

   Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

   Insert

   HIS3
   

   Use

   Synthetic Biology

   Available Since

   Feb. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV6- Entry-RPL26DC-DDK

   Plasmid

   #133867

   Purpose

   Expression of C-terminally FLAG-tagged human RPL26 mutant lacking the C-terminal UFMylation sites

   Depositor

   Yihong Ye

   Article

   Wang et al Cell Res. 2020 Jan;30(1):5-20. doi: 10.1

   Insert

   RPL26DC-myc-FLAG (RPL26 Human)
   

   Tags

   Myc-FLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. Lox-stop-Lox p53 R270H

   Plasmid

   #14853

   Depositor

   Tyler Jacks

   Article

   Olive et al Cell. 2004 Dec 17. 119(6):847-60.

   Insert

   p53 R270H (Trp53 Mouse)
   

   Use

   Cre/Lox

   Tags

   Lox-STOP-lox

   Expression

   Mammalian

   Mutation

   Contact mutant R270H. (Murine p53 codon 270 corre…

   Available Since

   April 27, 2007

   Availability

   Academic Institutions and Nonprofits only

10. px330-UFL1 sgRNA1

    Plasmid

    #134635

    Purpose

    contains sgRNA targeting human UFL1 for gene knockout

    Depositor

    Yihong Ye

    Article

    Wang et al Cell Res. 2020 Jan;30(1):5-20. doi: 10.1

    Insert

    UFL1 sgRNA1 (UFL1 Human)
    

    Expression

    Mammalian

    Available Since

    Dec. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pYpet-Optop38i

    Plasmid

    #89749

    Purpose

    Expression of light-regulated p38 inhibitor, Ypet-fused, wild-type photosensor, inhibitor MK3BD3-13F

    Depositor

    Michael Courtney

    Article

    Melero-Fernandez de Mera et al Nat Commun. 2017 May 12;8:15017. doi: 10

    Insert

    Optop38i3
    

    Use

    Fluorescent protein

    Tags

    Ypet

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGL4.24-Cd36-enhancer4

    Plasmid

    #138576

    Purpose

    Firefly luciferase enhancer reporter fused with 1.5~2 kb fragments of mouse Cd36 enhancers at Chr5:17876439_17877752

    Depositor

    Lora Hooper

    Article

    Kuang et al Science. 2019 Sep 27;365(6460):1428-1434

    Insert

    Cd36 Promoter (Cd36 Mouse)
    

    Use

    Luciferase

    Promoter

    Minimal promoter

    Available Since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pENTR223-BGLF5

    Plasmid

    #195813

    Purpose

    Gateway Entry Clone of Epstein-Barr virus BGLF5

    Depositor

    Eric Johannsen

    Article

    Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

    Insert

    BGLF5 (BGLF5 Epstein-Barr virus)
    

    Use

    Gateway entry vector

    Available Since

    May 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.1-dCas9-MQ1(C141S)-EGFP

    Plasmid

    #89635

    Purpose

    Targeted CpG methylation

    Depositor

    Margaret Goodell

    Article

    Lei et al Nat Commun. 2017 Jul 11;8:16026. doi: 10

    Insert

    site-specific DNA-methyltransferase SssI
    

    Use

    CRISPR

    Tags

    3*FLAG, 6*His, and T2A-EGFP

    Expression

    Mammalian

    Mutation

    C141S, TGC-->TCC

    Promoter

    CMV

    Available Since

    July 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET21-pelB-LaG-17

    Plasmid

    #172747

    Purpose

    Bacterial expression of HIS-tagged anti-GFP nanobody LaG-17; HIS tag preceded by HRV 3C/PreScission cleavage site.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaG-17
    

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3-AMPKsub(TA)-YPet-NES

    Plasmid

    #84636

    Purpose

    Encodes negative-control C-terminal (substrate) fragment of bimolecular AMPK/BRSK activity reporter (bimABKAR); cytosol targeted; use in conjunction with pcDNA3-Cerulean-FHA1-NES

    Depositor

    Jin Zhang

    Article

    Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do

    Insert

    AMPKsub(TA)-YPet-NES
    

    Tags

    6xHis, Nuclear export signal (NES), T7 tag (gene …

    Expression

    Mammalian

    Mutation

    Target Thr residue in substrate domain mutated to…

    Promoter

    CMV

    Available Since

    April 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGEL636

    Plasmid

    #189830

    Purpose

    The T-DNA backbone of Cas12j2 for transcriptional activation in rice

    Depositor

    Yong Zhang

    Article

    Liu et al Plant Commun. 2022 Sep 20:100453. doi: 1

    Type

    Empty backbone

    Use

    CRISPR

    Promoter

    pZmUbi1

    Available Since

    April 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGEL638

    Plasmid

    #189831

    Purpose

    The T-DNA backbone of nCas12j2 for genome editing in rice

    Depositor

    Yong Zhang

    Article

    Liu et al Plant Commun. 2022 Sep 20:100453. doi: 1

    Type

    Empty backbone

    Use

    CRISPR; T-dna clone

    Promoter

    ZmUbi1

    Available Since

    April 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pD2529-CAG-aIIb

    Plasmid

    #189796

    Purpose

    Expression of full length human integrin aIIb

    Depositor

    Timothy Springer

    Article

    Lin et al Cell. 2022 Sep 15;185(19):3533-3550.e27.

    Insert

    aIIb (ITGA2B Human)
    

    Expression

    Mammalian

    Available Since

    Sept. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pENTR-ATXN1

    Plasmid

    #16133

    Depositor

    Huda Zoghbi

    Article

    Lim et al Cell. 2006 May 19. 125(4):801-14.

    Insert

    Ataxin1 (ATXN1 Human)
    

    Expression

    Mammalian

    Available Since

    Jan. 18, 2008

    Availability

    Academic Institutions and Nonprofits only