We narrowed to 15,674 results for: NOL

1. ND4.2-Right DdCBE-G1397-C- T1413I

   Plasmid

   #179685

   Purpose

   Expression of base editor in mammalian cells

   Depositor

   David Liu

   Article

   Mok et al Nat Biotechnol. 2022 Sep;40(9):1378-1387

   Insert

   SOD2 MTS_3xHA_ND4.2 Right TALE_2aa_DddA G1397-C (T1413I)_1xUGI_SOD2 3'UTR
   

   Expression

   Mammalian

   Mutation

   T1413I

   Available Since

   April 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDonor14-AcHELIX-KanR (CJT96)

   Plasmid

   #181794

   Purpose

   pDonor for AcHELIX containing 14bp of spacing between the I-AniI site and LE/RE using ShCAST flanking sequence and encoding a kanamycin resistance gene as cargo

   Depositor

   Benjamin Kleinstiver

   Article

   Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

   Insert

   KanR, R6K origin of replication
   

   Expression

   Bacterial

   Available Since

   March 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEC3105 (pSpy1C-PxylS2_ffluc)

   Plasmid

   #218512

   Purpose

   replicative E. coli-S. pyogenes shuttle plasmid, constitutive expression of firefly luciferase reporter

   Depositor

   Emmanuelle Charpentier

   Article

   Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1

   Insert

   ffluc
   

   Use

   Luciferase

   Mutation

   deletion of Plac_mrfp cassette

   Promoter

   PxylS2

   Available Since

   Sept. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMC_MS2_DNA_VLP

   Plasmid

   #179156

   Purpose

   Expression of maturation protein and his-tagged coat protein dimer from MS2 phage

   Depositor

   Paul Freemont

   Article

   Crone et al GEN Biotechnol. 2022 Dec 1;1(6):496-503.

   Inserts

   Maturation Protein
   Coat Protein Dimer
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Sept. 6, 2022

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pCellFree_G03 CYP2D6

   Plasmid

   #67090

   Purpose

   Standard for cell-free expression benchmarking.

   Depositor

   Kirill Alexandrov

   Article

   Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

   Insert

   CYP2D6 (CYP2D6 Human)
   

   Use

   Cell-free expression

   Tags

   EGFP and His

   Promoter

   T7

   Available Since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCoofy57

   Plasmid

   #122009

   Purpose

   Mammalian vector for parallel SLIC cloning containing the VEGF signal sequence for secretion, N-terminal His, PreScission Cleavage site and different C-tag options

   Depositor

   Sabine Suppmann

   Article

   Scholz et al BMC Biotechnol. 2013 Feb 14;13(1):12.

   Type

   Empty backbone

   Tags

   His8 and Multi1: depending on the LP2 primer, eit…

   Expression

   Mammalian

   Available Since

   May 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBTK1009 (pSL9)

   Plasmid

   #191004

   Purpose

   Expresses E2 Crimson on a broad host range pBBR1 origin plasmid

   Depositor

   Jeffrey Barrick

   Article

   Elston et al ISME Commun. 2023 May 24;3(1):49. doi: 1

   Insert

   E2-Crimson
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   CP25

   Available Since

   Oct. 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. JD631

   Plasmid

   #160399

   Purpose

   Binary vector pGWB14 including Vitis GRF4-GIF1 under 35S promoter/ Plant transformation

   Depositor

   Jorge Dubcovsky

   Article

   Debernardi et al Nat Biotechnol. 2020 Oct 12. pii: 10.103

   Insert

   35S::vitis GRF4-GIF1 chimera
   

   Use

   Binary vector

   Available Since

   Nov. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pOCC151

   Plasmid

   #118899

   Purpose

   shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal GST, cleavable with 3C

   Depositor

   Marc Bickle, PEPC MPI CBG

   Article

   Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

   Insert

   NcoI-GST-3C-NotI-ccdB-AscI-stop-HindIII cassette
   

   Tags

   GST tag, cleavable with 3C protease

   Expression

   Insect

   Promoter

   polH

   Available Since

   Jan. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. SpCas9 nickase for Split-PE (pJUL2497)

    Plasmid

    #190104

    Purpose

    Expresses nickase SpCas9(H840A) for Split-PE use

    Depositor

    Keith Joung

    Article

    Grunewald et al Nat Biotechnol. 2023 Mar;41(3):337-343.

    Insert

    nSpCas9
    

    Tags

    XTEN-bpNLS and bpNLS

    Expression

    Mammalian

    Mutation

    H840A

    Promoter

    CMV

    Available Since

    Sept. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMB1-spect-PthrC3_8-eGFP

    Plasmid

    #107411

    Purpose

    This plasmid contain promoter PthrC3_8, which is used for study about Short, Auto-inducible Promoters for Well-Controlled Protein Expression in Escherichia coli.

    Depositor

    Kang Zhou

    Article

    Anilionyte et al Appl Microbiol Biotechnol. 2018 Aug;102(

    Insert

    eGFP
    

    Expression

    Bacterial

    Promoter

    PthrC3_8

    Available Since

    May 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTF-lacZ

    Plasmid

    #153036

    Purpose

    For CRISPR-Cas12a genome editing in E. coli for the insertion of large DNA fragments at the lacZ locus. Contains a constitutively expressed CRISPR array targeting the lacZ gene . No cargo DNA.

    Depositor

    Nigel Scrutton

    Article

    Jervis et al Microb Biotechnol. 2021 Mar 12. doi: 10.

    Insert

    CRISPR array
    

    Expression

    Bacterial

    Available Since

    May 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMJA179

    Plasmid

    #128537

    Purpose

    Expresses anti-Prunus dulcis scFv Ab

    Depositor

    Marcos Alcocer

    Article

    de la Cruz et al J Biotechnol. 2016 Jun 10;227:112-9. doi

    Insert

    scFv Ab clone PD1F6
    

    Expression

    Yeast

    Promoter

    AOX1

    Available Since

    July 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pUC18/promEco/Lysostaphin

    Plasmid

    #125765

    Purpose

    Production of staphylolytic protein lysostaphin from a constitutive promoter

    Depositor

    Benedykt Wladyka

    Article

    Madry et al Mol Biotechnol. 2019 Oct;61(10):774-782.

    Insert

    lysostaphin
    

    Expression

    Bacterial

    Mutation

    sequence corresponding to the mature form protein

    Promoter

    pemIK-Sa1 promoter

    Available Since

    Oct. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pU6-gRNA

    Plasmid

    #53062

    Purpose

    Transcription of guide RNA (gRNA) in wheat cells

    Depositor

    Caixia Gao

    Article

    Shan et al Nat Biotechnol. 2013 Aug;31(8):686-8. do

    Insert

    wheat U6 promoter - gRNA
    

    Use

    CRISPR; Plant expression

    Promoter

    wheat U6

    Available Since

    Aug. 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGL3-mArg1 base promoter -31/-2365

    Plasmid

    #34570

    Depositor

    Peter Murray

    Article

    Pauleau et al J Immunol. 2004 Jun 15;172(12):7565-73.

    Insert

    Arg1 base promoter -31/-2365 (Arg1 Mouse)
    

    Use

    Luciferase

    Tags

    Firefly Luciferase

    Available Since

    Dec. 21, 2011

    Availability

    Academic Institutions and Nonprofits only

17. pCMT-flp

    Plasmid

    #67274

    Purpose

    Conjugally transferable Flp recombinase plasmid for unmarked mutagenesis

    Depositor

    Mark Liles

    Article

    Hossain et al Biotechnol Rep (Amst). 2015 Aug 28;8:24-

    Insert

    tetA, flp, repA101, oriR101
    

    Use

    Flp recombinase plasmid, temperature sensitive

    Tags

    His-SUMO (flp)

    Promoter

    tetA promoter of plasmid pACYC184 origin

    Available Since

    Aug. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pQE-80L 2xHis-Rab29

    Plasmid

    #186021

    Purpose

    2xHis-Rab29

    Depositor

    Suzanne Pfeffer

    Article

    Vides et al Elife. 2022 Sep 23;11:e79771. doi: 10.75

    Insert

    2xHis-Rab29 (RAB29 Human)
    

    Expression

    Bacterial

    Available Since

    Sept. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. flex-ChrimsonR-EYFP-Kv

    Plasmid

    #135319

    Purpose

    Cre-dependent expression of soma-targeted ChrimsonR

    Depositor

    Hongkui Zeng

    Article

    Hage et al Elife. 2022 Jan 21;11:e71103. doi: 10.75

    Insert

    ChrimsonR
    

    Use

    AAV

    Tags

    EYFP

    Promoter

    synapsin

    Available Since

    Jan. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pHSB04X

    Plasmid

    #117258

    Purpose

    Genome editing for Lactobacillus brevis ATCC367

    Depositor

    Sheng Yang

    Article

    Huang et al Biotechnol J. 2019 Jul;14(7):e1800690. d

    Insert

    Cas9, promotor PslpA-guide-RNA, homologous arms of Lb_1019
    

    Use

    Other

    Available Since

    Sept. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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