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VV220: CoChR(C108S)-eGFP in fck

Plasmid

#58512

Purpose

Expresses CoChR with the C108S (step function opsin) mutation fused to eGFP under the a-CamKII promoter

Depositor

Adam Cohen

Article

Venkatachalam et al Biophys J. 2014 Oct 7;107(7):1554-63. do

Insert

CoChR(C108S)-eGFP

Use

Lentiviral

Tags

eGFP

Expression

Mammalian

Mutation

changed Cysteine 108 to Serine

Promoter

a-CamKII

Available Since

Nov. 5, 2014

Availability

Academic Institutions and Nonprofits only

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p5E-Ui4-eSIBR

Plasmid

#82586

Purpose

5'entry vector containing human ubiquitin C promoter followed by intronically-expressed eSIBR cassette for ubiquitous expression, RNAi

Depositor

Kryn Stankunas

Article

Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

Insert

human ubiquitin C promoter followed by intronically expressed eSIBR cassette

Promoter

None

Available Since

Dec. 21, 2016

Availability

Academic Institutions and Nonprofits only

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pMuLE ENTR U6 L1-L4

Plasmid

#62112

Purpose

MuLE (Multiple Lentiviral Expression) Entry vector containing a U6 promoter for shRNA expression. Compatible with MultiSite Gateway cloning

Depositor

Ian Frew

Article

Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

Type

Empty backbone

Use

RNAi; Mule gateway entry vector

Expression

Mammalian

Available Since

Feb. 26, 2015

Availability

Academic Institutions and Nonprofits only

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psiCHECK-2-Ywhag-3'UTR

Plasmid

#61778

Purpose

Luciferase reporter containing the 3'UTR of mouse Ywhag

Depositor

Judy Lieberman

Article

Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

Insert

mouse Ywhag 3'UTR

Use

Luciferase

Available Since

April 20, 2016

Availability

Academic Institutions and Nonprofits only
pcDNA3_3_TOPO_TA-ms2-VP64

Plasmid

#79371

Purpose

Recruits VP64 to a compatible gRNA for transcriptional activation

Depositor

George Church

Article

Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

Insert

MS2-VP64

Use

CRISPR

Expression

Mammalian

Available Since

June 27, 2017

Availability

Academic Institutions and Nonprofits only

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pENTR221-WNT3A

Plasmid

#79501

Purpose

MultiSite Gateway entry clones, second fragment (attL5, attL2) Human WNT3A CDS

Depositor

Peter Jon Nelson

Article

Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

Insert

WNT3A (WNT3A Human)

Use

Multisite gateway entry clone

Promoter

no Promoter

Available Since

May 12, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-SMVP-Cas9N-U6-gRNA M4

Plasmid

#80937

Purpose

Expresses Cas9N in mammalian cells; expresses gRNA M4 for Mstn cleavage.

Depositor

George Church

Article

Chew et al Nat Methods. 2016 Sep 5. doi: 10.1038/nm

Insert

Cas9N

Use

AAV, CRISPR, and Synthetic Biology

Expression

Mammalian

Available Since

Oct. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pAct:MCP-VP64

Plasmid

#78904

Purpose

Expresses MCP:VP64 under actin promoter for "Scaffold" CRISPRa in Drosophila cells

Depositor

Norbert Perrimon

Article

Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

Insert

MS2:VP64

Use

CRISPR

Expression

Insect

Promoter

pActin (Drosophila)

Available Since

March 25, 2017

Availability

Academic Institutions and Nonprofits only

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psiCHECK-2-Xbp1-3'UTR

Plasmid

#61779

Purpose

Luciferase reporter containing the 3'UTR of mouse Xbp1

Depositor

Judy Lieberman

Article

Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

Insert

mouse Xbp1 3'UTR

Use

Luciferase

Available Since

April 20, 2016

Availability

Academic Institutions and Nonprofits only
pU6_(GLuc)_INT[GFPApt]

Plasmid

#68428

Purpose

Transient expression in mammalian cells of an "INT" construct_bearing the GFP aptamer, targeting the GLuc reporter. U6 promoter

Depositor

John Rinn

Article

Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

Insert

INT construct_bearing the GFP aptamer

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Promoter

human U6

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only

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pET15b+PCNA3 WT

Plasmid

#66179

Purpose

Expresses the wild type of Sulfolobus solfataricus PCNA3

Depositor

Teruyuki Nagamune

Article

Hirakawa et al Biotechnol Bioeng. 2013 Jul;110(7):1858-

Insert

DNA polymerase sliding clamp 3

Tags

His6 tag

Expression

Bacterial

Promoter

T7 promoter

Available Since

June 30, 2015

Availability

Academic Institutions and Nonprofits only

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pET15b+PCNA2 WT

Plasmid

#66175

Purpose

Expresses the wild type of Sulfolobus solfataricus PCNA2

Depositor

Teruyuki Nagamune

Article

Hirakawa et al Biotechnol Bioeng. 2013 Jul;110(7):1858-

Insert

DNA polymerase sliding clamp 2

Tags

His6 tag

Expression

Bacterial

Promoter

T7 promoter

Available Since

June 30, 2015

Availability

Academic Institutions and Nonprofits only

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pU6_(GLuc)_INT[3xK-T]

Plasmid

#68429

Purpose

Transient expression in mammalian cells of an "INT" construct_bearing three kink-turns, targeting the GLuc reporter. U6 promoter

Depositor

John Rinn

Article

Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

Insert

INT construct_bearing three kink-turns

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Promoter

human U6

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only

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pGSTag BimEL (T112A)

Plasmid

#23101

Depositor

Roger Davis

Article

Hubner et al Mol Cell. 2008 May 23. 30(4):415-25.

Insert

Bim EL (TA) (Bcl2l11 Mouse)

Tags

Gst

Expression

Bacterial

Mutation

Changed Threonine 112 to Alanine

Available Since

Feb. 24, 2010

Availability

Academic Institutions and Nonprofits only
pGSTag BimEL (T112D)

Plasmid

#23102

Depositor

Roger Davis

Article

Hubner et al Mol Cell. 2008 May 23. 30(4):415-25.

Insert

Bim EL (TD) (Bcl2l11 Mouse)

Tags

Gst

Expression

Bacterial

Mutation

Changed Threonine 112 to Aspartate

Available Since

Feb. 24, 2010

Availability

Academic Institutions and Nonprofits only
pMuLE ENTR H1 L5-L2

Plasmid

#62103

Purpose

MuLE (Multiple Lentiviral Expression) Entry vector containing a H1 promoter for shRNA expression. Compatible with MultiSite Gateway cloning

Depositor

Ian Frew

Article

Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

Type

Empty backbone

Use

RNAi; Mule gateway entry vector

Expression

Mammalian

Available Since

Feb. 11, 2015

Availability

Academic Institutions and Nonprofits only

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Rc/CMV cyclin D2 K112E HA

Plasmid

#8953

Depositor

Philip Hinds

Article

Baker et al Cell Cycle. 2005 Feb . 4(2):330-8.

Insert

cyclin D2 (CCND2 Human)

Tags

HA

Expression

Mammalian

Mutation

K112E: point mutation in cyclin box

Available Since

Oct. 6, 2005

Availability

Academic Institutions and Nonprofits only
pCAG-aptamer array

Plasmid

#236387

Purpose

crRNA array expression

Depositor

Ling-Ling Chen

Article

Yang et al Nat Methods. 2024 Sep;21(9):1646-1657. d

Insert

Aptamer array

Use

CRISPR and Lentiviral

Available Since

Dec. 10, 2025

Availability

Academic Institutions and Nonprofits only

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1M-Qt-eUnaG-TGA-rt20s-anti-mCherry(LaM-4)

Plasmid

#238894

Purpose

EMcapsulin class with a eUnaG fusion and an anti-mCherry intrabody (LaM-4) fusion preceded by a TGA stop codon and the rt20s readthrough motif.

Depositor

Gil Westmeyer

Article

Berezin et al Nat Protoc. 2025 Nov 19. doi: 10.1038/s4

Insert

1M-Qt-eUnaG-TGA-rt20s-anti-mCherry(LaM-4)

Use

Affinity Reagent/ Antibody

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 1, 2025

Availability

Academic Institutions and Nonprofits only

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HP17 sfGFP::P8

Plasmid

#235448

Purpose

Helper plasmid with sfGFP gene inserted at gene VIII locus

Depositor

Yolanda Schaerli

Article

Kusumawardhani et al Nat Commun. 2025 Apr 15;16(1):3569. doi:

Insert

Coding part of the M13KO7 helper phage genome, except gene VIII

Expression

Bacterial

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

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