166,461 results

1. p-EF1a-CreERT2-3Xflag-T2A-eBFP2

   Plasmid

   #170186

   Purpose

   This Cre-ERT2 expressing construct can be used to inducibly recombine loxp sites. It can be used with poly-loxP containing plasmids to generate timestamp barcodes useful for linage tracing

   Depositor

   Bradley Bernstein

   Article

   Kim et al Cell Rep. 2020 Oct 6;33(1):108222. doi:

   Insert

   CreERT2-T2A-eBFP2
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   EF1 alpha

   Available Since

   July 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pENN.AAV.CamKII.HI.GFP-Cre.WPRE.SV40 (AAV9)

   Viral Prep

   #105551-AAV9

   Purpose

   Ready-to-use AAV9 particles produced from pENN.AAV.CamKII.HI.GFP-Cre.WPRE.SV40 (#105551). In addition to the viral particles, you will also receive purified pENN.AAV.CamKII.HI.GFP-Cre.WPRE.SV40 plasmid DNA. Expression of GFP-Cre from CamKII promoter. These AAV preparations are suitable purity for injection into animals.

   Depositor

   James M. Wilson

   Promoter

   CaMKII

   Tags

   GFP

   Available Since

   Aug. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

3. Lenti-Hb9-GFP

   Plasmid

   #37080

   Purpose

   3rd generation lentiviral transfer plasmid for Hb9 GFP

   Depositor

   Fred Gage

   Article

   Marchetto et al Cell Stem Cell. 2008 Dec 4;3(6):649-57.

   Insert

   Hb9 GFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Hb9

   Available Since

   Oct. 15, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-Ef1a-DIO eNpHR 3.0-EYFP (AAV5)

   Viral Prep

   #26966-AAV5

   Purpose

   Ready-to-use AAV5 particles produced from pAAV-Ef1a-DIO eNpHR 3.0-EYFP (#26966). In addition to the viral particles, you will also receive purified pAAV-Ef1a-DIO eNpHR 3.0-EYFP plasmid DNA. EF1a-driven, cre-dependent eNpHR 3.0-EYFP for optogenetic inhibition. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Karl Deisseroth

   Article

   Gradinaru et al Cell. 2010 Apr 2. 141(1):154-65.

   Promoter

   EF1a

   Tags

   EYFP (Cre-dependent)

   Available Since

   Feb. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

5. pAct:dCas9-VPR

   Plasmid

   #78898

   Purpose

   Expresses dCas9-VPR under Actin promoter for CRISPRa in Drosophila cell culture. Please note- this plasmid does not express GFP.

   Depositor

   Norbert Perrimon

   Article

   Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

   Insert

   dCas9-VPR
   

   Use

   CRISPR

   Tags

   Expression

   Insect

   Mutation

   Promoter

   pActin (Drosophila)

   Available Since

   Aug. 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. CROPseq-EFS-HypaSpCas9-P2A-EGFP

   Plasmid

   #106964

   Purpose

   single-vector CROPseq system with high fidelity SpCas9 (HypaSpCas9) for use in the CROPseq CRISPR knockout screen

   Depositor

   Alex Hewitt

   Insert

   HypaSpCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EFS

   Available Since

   May 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMpGE014

   Plasmid

   #108682

   Purpose

   All-in-one genome editing vector in M. polymorpha (chlorsulfuron resistant)

   Depositor

   Shigeo Sugano, Takayuki Kohchi

   Article

   Sugano et al PLoS One. 2018 Oct 31;13(10):e0205117. d

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   June 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. 5` CC: Puro – loxP – GFP-C

   Plasmid

   #219563

   Purpose

   5` circularization cassette.Used in combination with one of the 3' circularization cassettes to induce Cre-mediated circularizazion or inversion of a desired genomic region.

   Depositor

   Andrea Ventura

   Article

   Pradella et al Nature. 2024 Dec 18. doi: 10.1038/s41586

   Insert

   hPGK-promoter_PuroR_2A_loxP_GFP-C
   

   Use

   Unspecified

   Tags

   Expression

   Mutation

   Promoter

   hPGK

   Available Since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET15-UbE2W

   Plasmid

   #15809

   Depositor

   Wade Harper

   Article

   Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

   Insert

   Ubiquitin conjugating enzyme UbE2W (UBE2W Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Sept. 28, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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10. PiggyBac PlayBack

    Plasmid

    #203312

    Purpose

    To all for PiggyBac gene insertion into Genome

    Depositor

    Aleksandar Necakov

    Article

    Foran et al Biotechniques. 2023 Oct;75(4):168-178. d

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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