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pEasyG2_zeo

Plasmid

#184913

Purpose

Template to generate via PCR two gRNAs for expression in S. cerevisiae. The PCR product from pEasyG2_zeo recombines in vivo with a PCR product from pEasyG2_mic.

Depositor

Jeferson Gross

Article

Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-

Insert

gRNA scaffold

Use

CRISPR

Expression

Bacterial

Available Since

Dec. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pEasyG2_nat

Plasmid

#184914

Purpose

Template to generate via PCR two gRNAs for expression in S. cerevisiae. The PCR product from pEasyG2_nat recombines in vivo with a PCR product from pEasyG2_mic.

Depositor

Jeferson Gross

Article

Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-

Insert

gRNA scaffold

Use

CRISPR

Expression

Bacterial

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pEasyG3_zeo

Plasmid

#184917

Purpose

Template to generate via PCR two gRNAs for expression in S. cerevisiae. The PCR product from pEasyG3_zeo recombines in vivo with a PCR product from pEasyG3_mic.

Depositor

Jeferson Gross

Article

Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-

Insert

gRNA scaffold

Use

CRISPR

Expression

Bacterial

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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EF1a_KLF14_P2A_Hygro_Barcode

Plasmid

#120500

Purpose

Barcoded lentiviral vector to express KLF14 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

KLF14 (KLF14 Human)

Use

Lentiviral

Promoter

EF1a

Available Since

Feb. 6, 2019

Availability

Academic Institutions and Nonprofits only

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Topo_SpCas9.tracr_LbCas12a.DR

Plasmid

#155049

Purpose

TOPO vector for the cloning of _the SpCas9 tracrRNA - LbCas12a Direct Repeat (DR) fragment into the pLCHKO hgRNA vector

Depositor

Benjamin Blencowe, Jason Moffat

Article

Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

Insert

SpCas9 tracrRNA and LbCas12a Direct Repeat (DR)

Use

CRISPR

Available Since

Sept. 16, 2020

Availability

Academic Institutions and Nonprofits only

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Cx26-sfGFP

Plasmid

#69026

Purpose

Expresses rat Cx26 (Gjb2 CDS) with a 7 amino acid linker on the C-terminus of the Connexin linking to non-monomerized superfolder GFP. Expression driven by CMV promoter.

Depositor

David Spray

Article

Stout RF et al J Biol Chem. 2015 Aug 11. pii: jbc.M115.

Insert

Cx26 (Gjb2 Rat)

Tags

non-monomerized superfolder GFP (V206)

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 23, 2015

Availability

Academic Institutions and Nonprofits only

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CRY-tTA (B702)

Plasmid

#92032

Purpose

Expresses CRY2 (Full length, mutated NLS) fusion with tTA2 'tet-OFF' transcriptional activator

Depositor

Chandra Tucker

Article

Pathak et al Nucleic Acids Res. 2017 Apr 20. doi: 10.

Insert

CRY2 (CRY2 Mustard Weed)

Tags

tTA2 (TetR fused to VP64)

Expression

Mammalian

Mutation

NLS on CRY2 is mutated

Promoter

CMV

Available Since

July 25, 2017

Availability

Academic Institutions and Nonprofits only

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p1.2-GS

Plasmid

#162770

Purpose

Protein expression in CHO cells

Depositor

Ivan Vorobiev

Article

Kovnir et al Bull Exp Biol Med. 2017 Jun;163(2):245-2

Type

Empty backbone

Expression

Mammalian

Promoter

CHO EEF1A1 (Translation Elongation Factor 1 Alpha…

Available Since

Aug. 5, 2021

Availability

Academic Institutions and Nonprofits only

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LK4-Ets2

Plasmid

#44606

Depositor

Robert Oshima

Article

Galang et al J Biol Chem. 1996 Apr 5;271(14):7992-8.

Insert

Ets2 (Ets2 Mouse)

Expression

Mammalian

Promoter

human Beta-Actin

Available Since

May 13, 2013

Availability

Academic Institutions and Nonprofits only
pcDNA3.1 PA-NL

Plasmid

#113445

Purpose

ProteinA-NanoLuc hybrid fusion control expression vector (for BRET and LuC normalization)

Depositor

Erich Wanker

Article

Trepte et al Mol Syst Biol. 2018 Jul 11;14(7):e8071.

Insert

ProteinA-NanoLuc

Use

Luciferase

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 PA-mCit-NL

Plasmid

#113444

Purpose

ProteinA-mCitrine-NanoLuc hybrid-fusion control expression vector (positive control)

Depositor

Erich Wanker

Article

Trepte et al Mol Syst Biol. 2018 Jul 11;14(7):e8071.

Insert

ProteinA-mCitrine-NanoLuc

Use

Luciferase

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 10, 2018

Availability

Academic Institutions and Nonprofits only

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HTRA3_PDZ_1

Plasmid

#103914

Purpose

Protein expression and purification of HTRA3 PDZ domain

Depositor

Sachdev Sidhu

Article

Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

Insert

HTRA3 (HTRA3 Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

Aug. 29, 2018

Availability

Academic Institutions and Nonprofits only

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PD-L1-miSFIT-6-C, 16-C

Plasmid

#124689

Purpose

Tuning expression of PD-1

Depositor

Tudor Fulga

Article

Michaels et al Nat Commun. 2019 Feb 18;10(1):818. doi:

Insert

PD-L1 (CD274 Human)

Expression

Mammalian

Promoter

minimal CMV

Available Since

May 8, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti PA-mCit-GW

Plasmid

#113456

Purpose

Lentiviral ProteinA-mCitrine Gateway shuttle vector for N-terminal fusions

Depositor

Erich Wanker

Article

Trepte et al Mol Syst Biol. 2018 Jul 11;14(7):e8071.

Type

Empty backbone

Use

Lentiviral

Tags

ProteinA-mCitrine

Expression

Mammalian

Promoter

hUbC

Available Since

Aug. 14, 2018

Availability

Academic Institutions and Nonprofits only

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p3E DN Kif5a

Plasmid

#105973

Purpose

3' element for Gateway cloning, 5 single nucleotide mutations compared to the reference sequence, but none of them change the amino acidtruncation aa416-1033(end) 1854 bp

Depositor

Caren Norden

Article

Taverna et al Sci Rep. 2016 Feb 16;6:21206. doi: 10.10

Insert

Kif5a (kif5aa Zebrafish)

Expression

Bacterial

Mutation

truncation of aa 416-1033

Available Since

Feb. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_APLP2_Kunitz-BPTI

Plasmid

#110041

Purpose

Protein expression and purification of APLP2_Kunitz-BPTI

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

APLP2_Kunitz-BPTI (APLP2 Human)

Expression

Bacterial

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pSF3-Flag-Cbir-Cdc25C S216A_Myc-Nbir-14-3-3e

Plasmid

#90011

Purpose

Split-BioID plasmid: Expresses N-terminally tagged CBir[257-321]-Cdc25C S216A and N-terminally tagged NBir[2-256]-14-3-3e

Depositor

Julien Béthune

Article

Schopp et al Nat Commun. 2017 Jun 6;8:15690. doi: 10.

Inserts

CBirA*-Cdc25C S216A
NBirA*-14-3-3e

Use

Retroviral; Flp/frt

Tags

FLAG and Myc

Expression

Mammalian

Mutation

Ser 216 to Ala

Promoter

CMV

Available Since

Jan. 2, 2018

Availability

Academic Institutions and Nonprofits only

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EGFP-CCHD (p130Cas)

Plasmid

#145132

Purpose

Expresses CCH domain from p130Cas with an EGFP tag

Depositor

Johanna Ivaska

Article

Jacquemet et al Curr Biol. 2019 Jan 21;29(2):202-216.e7.

Insert

p130Cas CCH domain (BCAR1 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

July 28, 2020

Availability

Academic Institutions and Nonprofits only

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pWZLblasti-HA-MEK5

Plasmid

#53167

Purpose

Expresses HA tagged human MEK5 from blasticidin resistance retroviral vector

Depositor

Christopher Counter

Article

Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

Insert

MEK5 (MAP2K5 Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Available Since

June 24, 2014

Availability

Academic Institutions and Nonprofits only
pmiRGLO-LFNG-203

Plasmid

#117351

Purpose

Dual luciferase assay for miRNA-targeted UTR

Depositor

Volker Busskamp

Article

Kutsche et al Cell Syst. 2018 Sep 28. pii: S2405-4712(

Insert

3' UTR LFNG-203

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Promoter

Murine PGK-1

Available Since

Dec. 19, 2018

Availability

Academic Institutions and Nonprofits only

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