We narrowed to 34,882 results for: cas

1. Zebrafish-gRNA-0008

   Plasmid

   #42248

   Purpose

   gRNA targeted to zebrafish gene tia1l

   Depositor

   Keith Joung

   Article

   Hwang et al Nat Biotechnol. 2013 Jan 29. doi: 10.103

   Insert

   gRNA-tia1l (tia1l Zebrafish)
   

   Use

   CRISPR; Zebrafish expression

   Tags

   Expression

   Mutation

   Promoter

   T7

   Available since

   Jan. 31, 2013

   Availability

   Academic Institutions and Nonprofits only

2. pSL0721 (pDonor_L1-105)

   Plasmid

   #130645

   Purpose

   Encodes a mini-transposon derived from V. cholerae Tn6677 CAST, with CmR cargo gene and shortened left end. Total transposon size = 933 bp.

   Depositor

   Samuel H. Sternberg

   Article

   Klompe et al Nature. 2019 Jul;571(7764):219-225. doi:

   Insert

   VchCAST donor DNA (short L)
   

   Use

   CRISPR; Transposon

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCsy-crRNA-EV

   Plasmid

   #153944

   Purpose

   For cloning and expression of Type I-F P. aeruginosa (PaeCascade) crRNA in mammalian cells

   Depositor

   Zhou Songyang

   Article

   Chen et al Nat Commun. 2020 Jun 19;11(1):3136. doi:

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSL0376 (His-MBP-StrepII-TniQ)

   Plasmid

   #130641

   Purpose

   Expresses V. cholerae CAST TniQ from a T7 promoter, with N-terminal His10-MBP-TEVsite-StrepII for purification.

   Depositor

   Samuel H. Sternberg

   Article

   Klompe et al Nature. 2019 Jul;571(7764):219-225. doi:

   Insert

   VchTniQ
   

   Use

   CRISPR; Transposon

   Tags

   His10-MBP-TEVsite-StrepII on TniQ

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLentiCRISPR sgSLC19A1

   Plasmid

   #102314

   Purpose

   genetic depletion of SLC19A1

   Depositor

   David Sabatini

   Article

   Kanarek et al Nature. 2018 Jul 11. pii: 10.1038/s41586

   Insert

   sgSLC19A1 (SLC19A1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   July 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSL0715 (pDonor_R1-57)

   Plasmid

   #130644

   Purpose

   Encodes a mini-transposon derived from V. cholerae Tn6677 CAST, with CmR cargo gene and shortened right end. Total transposon size = 910 bp.

   Depositor

   Samuel H. Sternberg

   Article

   Klompe et al Nature. 2019 Jul;571(7764):219-225. doi:

   Insert

   VchCAST donor DNA (short R)
   

   Use

   CRISPR; Transposon

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pKDsgRNA-pfkB

   Plasmid

   #102284

   Purpose

   Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting pfkB.

   Depositor

   Michael Lynch

   Article

   Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

   Insert

   pfkB gRNA
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pTet

   Available since

   April 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. EF1a-Triplex-28-M13-28-pA

   Plasmid

   #202041

   Purpose

   Encodes a non-coding transcript expressed from the EF1a promoter that has a 3' UTR containing a MALAT1 triplex, Csy4 binding sites ("28"), and a cloning site for a Cas9 gRNA.

   Depositor

   Darren Cusanovich

   Article

   Kang et al ACS Synth Biol. 2025 May 16;14(5):1732-1

   Insert

   Triplex-28-M13-28 (noncoding)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLentiCRISPR-v1-sgNADK2

   Plasmid

   #233897

   Purpose

   lentiviral vector expressing Cas9 and an sgRNA targeting NADK2

   Depositor

   Jason Cantor

   Article

   Flickinger et al Nat Metab. 2025 May 2. doi: 10.1038/s422

   Insert

   sgRNA targeting NADK2 (NADK2 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   May 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAPH07

    Plasmid

    #234345

    Purpose

    Library-scale IPTG-inducible gRNA expression for Type II dCas9 in Synechococcus sp. PCC 7002, genomically integrated next to glpK

    Depositor

    Jerome Fox

    Article

    Hren et al Proc Natl Acad Sci U S A. 2025 Mar 25;12

    Insert

    Type II dCas9 sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    lacUV5

    Available since

    April 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAPH021

    Plasmid

    #234346

    Purpose

    Library-scale IPTG-inducible single transcript dual-gRNA expression for Type II dCas9 in Synechococcus sp. PCC 7002, genomically integrated next to glpK

    Depositor

    Jerome Fox

    Article

    Hren et al Proc Natl Acad Sci U S A. 2025 Mar 25;12

    Inserts

    Type II dCas9 sgRNA
    Type II dCas9 sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    lacUV5

    Available since

    April 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTBL3346 PB-nicking guide-blast

    Plasmid

    #226665

    Purpose

    PiggyBac cargo vector encoding protective nicking sgRNA marked with blasticidin

    Depositor

    Chang Liu

    Article

    Loveless et al bioRxiv 2021.11.05.467507

    Insert

    protective nicking sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    Oct. 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLenti-Sa-gRNA-puro

    Plasmid

    #191652

    Purpose

    Lentiviral expression of puromycin resistance gene and S. aureus scrambled non-targeting gRNA ; useful for changing gRNA by mutagenesis.

    Depositor

    Moshe Szyf

    Article

    Sapozhnikov et al Sci Rep. 2023 Feb 9;13(1):2280. doi: 10.

    Insert

    S. aureus gRNAscr
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Nov. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLentiguide-puro_hNKD2sg#1

    Plasmid

    #174323

    Purpose

    gRNA targeting hNKD2 - encodes puromycin resistance

    Depositor

    Sonal Srikanth

    Article

    Wu et al Cell Rep. 2021 Aug 24;36(8):109603. doi:

    Insert

    human NKD2
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pJK528

    Plasmid

    #155388

    Purpose

    dCas12a oscillator, reverse crRNAs + PA4-mVenus. (Integration of 511; pOSIP)

    Depositor

    Pamela Silver

    Article

    Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81

    Inserts

    dCas12a (F. novicida)
    PA4-mVenus
    dCas12a oscillator crRNAs
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    D917A (nuclease-deactivating)

    Promoter

    Available since

    Feb. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pJK434

    Plasmid

    #155378

    Purpose

    PphlF_A4NT, 1x cascade + [dCas9 + PA4-mVenus]. (Integration of 429; pOSIP)

    Depositor

    Pamela Silver

    Article

    Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81

    Inserts

    dCas9 (bacteria)
    PA4-mVenus
    PphlF_A4NT in dCas9 sgRNA oscillator v1
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    D10A H840A (catalytically inactive)

    Promoter

    Available since

    Feb. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pJK422

    Plasmid

    #155373

    Purpose

    dCas9 oscillator v1 (dCRv1) + [dCas9 + PA4-mVenus]. (Integration of 412; pOSIP)

    Depositor

    Pamela Silver

    Article

    Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81

    Inserts

    dCas9 (bacteria)
    PA4-mVenus
    dCas9 sgRNA oscillator v1
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    D10A H840A (catalytically inactive)

    Promoter

    Available since

    Dec. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pJK423

    Plasmid

    #155374

    Purpose

    dCas9 oscillator v1 (dCRv1) + [dCas9 + PA4-mVenus]. (Integration of 413; pOSIP)

    Depositor

    Pamela Silver

    Article

    Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81

    Inserts

    dCas9 (bacteria)
    PA4-mVenus
    dCas9 sgRNA oscillator v1
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    D10A H840A (catalytically inactive)

    Promoter

    Available since

    Dec. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLenti_CMV_CapChR2-GFP

    Plasmid

    #224357

    Purpose

    Expresses CapChR2-GFP in mammalian cells

    Depositor

    Adam Cohen

    Article

    Hayward et al Cell Calcium. 2024 Dec 5;125:102983. doi

    Insert

    CapChR2
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pME.A (pDPL6)

    Plasmid

    #24571

    Depositor

    David Pulleyblank

    Article

    Haniford et al Nature. 1983 Apr 14. 302(5909):632-4.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 3, 2010

    Availability

    Academic Institutions and Nonprofits only