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MEIS1B-pSP65

Plasmid

#21019

Depositor

Corey Largman

Article

Shen et al Mol Cell Biol. 1997 Nov;17(11):6448-58

Insert

MEIS1B (Meis1 Mouse)

Use

In vitro tnt

Tags

Flag

Available Since

Aug. 14, 2009

Availability

Academic Institutions and Nonprofits only
pLyGo-Kp-2

Plasmid

#163144

Purpose

pLyGo cloning vector for a sequence of interest (LPMO) in K. phaffii. Vector encoding the alfa-amylase signal peptide and the LyGo cassette (SapI-ccdB-SapI)

Depositor

Morten Norholm

Article

Hernandez-Rollan et al ACS Synth Biol. 2021 Apr 16;10(4):897-90

Type

Empty backbone

Use

Synthetic Biology

Expression

Yeast

Promoter

AOX1

Available Since

May 7, 2021

Availability

Academic Institutions and Nonprofits only

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pOME0006

Plasmid

#178375

Purpose

Expresses KSHV K2 ORF tagged

Depositor

Dirk Dittmer

Article

Alzhanova et al PLoS Pathog. 2021 Jan 7;17(1):e1009033.

Insert

KSHV ORF K2

Tags

3xFLAG

Expression

Mammalian

Available Since

Jan. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pME-hygro-hPGAP3-3HA

Plasmid

#50373

Purpose

Express C-terminally triple HA tagged human PGAP3 in mammmalian cells

Depositor

Reika Watanabe

Article

Castillon et al Mol Biol Cell. 2013 Jun;24(12):2021-33.

Insert

PGAP3 post-GPI attachment to proteins 3 (PGAP3 Human)

Tags

3x HA

Expression

Mammalian

Promoter

SR alpha

Available Since

July 15, 2014

Availability

Academic Institutions and Nonprofits only
actc1b-mKate2-LactC2

Plasmid

#109499

Purpose

Muscle specific zebrafish expression of mKate2 fused to the C-terminal domain from cow (Bos taurus) milk fat globue EGF-factor 8 protein (MFGE8). Binds phosphatidylserine (PS)

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

actc1b-mKate2-LactC2

Use

Zebrafish plasmids

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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actc1b-mKate2-rab7a

Plasmid

#109655

Purpose

Muscle specific zebrafish expression of mKate2 fused to rab7a

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

rab7a (rab7a Zebrafish)

Use

Zebrafish plasmids

Tags

mKate2

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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actc1b-mKate2-rab7b

Plasmid

#109656

Purpose

Muscle specific zebrafish expression of mKate2 fused to rab7b

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

rab7b (rab7b Zebrafish)

Use

Zebrafish plasmids

Tags

mKate2

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pMM1162

Plasmid

#112535

Purpose

pZE2-hrtR-RBS3-chuA - LuxCDABE transcriptional unit deletion of plasmid pMM643, ColE1 origin, KanR

Depositor

Timothy Lu

Article

Mimee et al Science. 2018 May 25;360(6391):915-918.

Inserts

HrtR
ChuA

Expression

Bacterial

Promoter

J23107 and ProD

Available Since

Feb. 6, 2019

Availability

Academic Institutions and Nonprofits only

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mIFP-MyosinIIC-N-18

Plasmid

#56232

Purpose

Localization: Cytoskeleton, Excitation: 683, Emission: 704

Depositor

Michael Davidson

Article

Yu et al Nat Methods. 2015 Aug;12(8):763-765. doi

Insert

MyosinIIC

Tags

mIFP

Expression

Mammalian

Promoter

CMV

Available Since

April 28, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pCMV Aquamarine-shadowY tandem

Plasmid

#157775

Purpose

Produces a fusion between Aquamarine and shadowY. It can be used as a positive control for FRET (using FLIM)

Depositor

Marc Tramier

Article

Bertolin et al eLife. 2018 Aug 2;7. pii: 38111. doi: 10

Type

Empty backbone

Tags

Aquamarine and shadowY

Expression

Mammalian

Available Since

Feb. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmLSm4_A

Plasmid

#145883

Purpose

Insect Expression of DmLSm4

Depositor

Elisa Izaurralde

Article

Tritschler et al Mol Cell Biol. 2007 Dec . 27(24):8600-11

Insert

DmLSm4 (LSm-4 Fly)

Tags

V5-6xHis

Expression

Insect

Mutation

two silent mutations compared to the sequence of …

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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actc1b-mKate2-rab8a

Plasmid

#109657

Purpose

Muscle specific zebrafish expression of mKate2 fused to rab8a

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

rab8a (rab8a Zebrafish)

Use

Zebrafish plasmids

Tags

mKate2

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pZ004 (LseC2c2 locus)

Plasmid

#70170

Purpose

Expresses LseC2c2 locus with one spacer in array

Depositor

Feng Zhang

Article

Shmakov et al Mol Cell. 2015 Nov 5;60(3):385-97. doi:

Insert

LseC2c2 locus with one spacer in array

Expression

Bacterial

Available Since

May 11, 2016

Availability

Academic Institutions and Nonprofits only
pME18S-F-R-hb2AR

Plasmid

#149367

Purpose

Expresses human b2AR in mammalian cells.

Depositor

Takeshi Imai

Article

Inagaki et al Cell Rep. 2020 Jun 30;31(13):107814. doi

Insert

b2AR (ADRB2 Human)

Tags

FLAG and rhodopsin

Expression

Mammalian

Promoter

SR alpha

Available Since

July 15, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV.U6gLacZ.hSyn.H2B.RFP

Plasmid

#170369

Purpose

Negative control plasmid used for Crispr/cas9 based disruption. Expressing nuclear RFP.

Depositor

Johan Jakobsson

Article

Jonsson et al EMBO J. 2021 Mar 1:e106423. doi: 10.1525

Insert

H2B RFP

Use

AAV

Promoter

human Synapsin

Available Since

Jan. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pEGFP-STAG2-A223Stop

Plasmid

#31973

Depositor

Todd Waldman

Article

Solomon et al Science. 2011 Aug 19;333(6045):1039-43.

Insert

human STAG2 cDNA (STAG2 Human)

Tags

EGFP

Expression

Mammalian

Mutation

changed Alanine 223 to Stop codon

Available Since

Aug. 25, 2011

Availability

Academic Institutions and Nonprofits only
Human T (BRACHYURY) sgRNA

Plasmid

#59726

Purpose

Plasmid encoding sgRNA to generate T (BRACHYURY) knock-out mutant human cells

Depositor

Jacob Hanna

Article

Irie et al Cell. 2015 Jan 15;160(1-2):253-68. doi:

Insert

hT sgRNA (TBXT Human)

Use

CRISPR

Expression

Mammalian

Available Since

Dec. 29, 2014

Availability

Academic Institutions and Nonprofits only
pUAST-HDAC4 3A

Plasmid

#52978

Purpose

insect expression of HDAC4 3A constitutive active mutant

Depositor

Marc Montminy, Biao Wang

Article

Wang et al Cell. 2011 May 13;145(4):596-606. doi: 1

Insert

HDAC4 (HDAC4 Fly)

Tags

FLAG

Expression

Insect

Mutation

S239,573,748A constitutively active*

Promoter

Hsp70

Available Since

July 29, 2014

Availability

Academic Institutions and Nonprofits only
Non-targeting CRISPRi plasmid

Plasmid

#154891

Purpose

Human non-targeting CRISPRi gRNA

Depositor

Joseph Dougherty, Rob Mitra

Article

Lalli et al Genome Res. 2020 Sep;30(9):1317-1331. do

Insert

Nontargeting gRNA

Use

CRISPR and Lentiviral

Available Since

Nov. 18, 2020

Availability

Academic Institutions and Nonprofits only

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HOXA9 (murine) FLAG pET

Plasmid

#8494

Depositor

Corey Largman

Article

Shen et al Mol Cell Biol 1997 Nov;17(11):6448-58.

Insert

HOXA9 (Hoxa9 Mouse)

Tags

FLAG and His

Expression

Bacterial

Mutation

FLAG epitope tag replaces T7 tag in the pET back…

Available Since

May 30, 2006

Availability

Academic Institutions and Nonprofits only