We narrowed to 25,266 results for: multi

1. pXEE401B

   Plasmid

   #91716

   Purpose

   CRISPR/Cas9-mediated genome editing in Arabidopsis. Contains Cas9 and empty gRNA scaffold, Bar resistance

   Depositor

   Qi-Jun Chen

   Article

   Wang et al Genome Biol. 2015 Jul 21;16:144. doi: 10

   Type

   Empty backbone

   Expression

   Plant

   Available Since

   July 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pmiRFP703-Tubulin

   Plasmid

   #79991

   Purpose

   Alpha tubulin labeling with near-infrared fluorescent protein miRFP703

   Depositor

   Vladislav Verkhusha

   Article

   Shcherbakova et al Nat Commun. 2016 Aug 19;7:12405. doi: 10

   Insert

   Tubulin (TUBA1B Human)
   

   Tags

   miRFP703

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pJFRC208-10XUAS-FRT>STOP>FRT-myr::smGFP-FLAG

   Plasmid

   #63169

   Purpose

   GAL4-responsive UAS “flp-On” spaghetti monster GFP-FLAG reporter

   Depositor

   Gerald Rubin

   Article

   Nern et al Proc Natl Acad Sci U S A. 2015 May 11. p

   Insert

   "spaghetti monster GFP-FLAG"
   

   Tags

   N-myristoylation

   Expression

   Insect

   Available Since

   May 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. RCAS-K-ras

   Plasmid

   #11549

   Depositor

   Sandra Orsulic

   Article

   Orsulic et al Cancer Cell. 2002 Feb . 1(1):53-62.

   Insert

   v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog (Kras Mouse)
   

   Use

   Retroviral

   Mutation

   G12D

   Available Since

   April 5, 2006

   Availability

   Academic Institutions and Nonprofits only

5. pU6_(GLuc)_INT[BoBS]

   Plasmid

   #68431

   Purpose

   Transient expression in mammalian cells of an "INT" construct_bearing the "Bunch of Baby Spinach" (or "BoBS") construct, targeting the GLuc reporter. U6 promoter

   Depositor

   John Rinn

   Article

   Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

   Insert

   INT construct_bearing the "Bunch of Baby Spinach" (or "BoBS") construct
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Mammalian

   Promoter

   human U6

   Available Since

   Oct. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pTU2-A-RFP (p15A origin)

   Plasmid

   #74090

   Purpose

   pTU2-A backbone, with RFP negative selection and p15A (low-copy) origin of replication

   Depositor

   Paul Freemont

   Article

   Moore et al ACS Synth Biol. 2016 May 2.

   Insert

   Golden Gate destination vector - Level 2, 4 TU's with RFP and p15A origin
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   R0010 Const. Promoter controlling RFP

   Available Since

   April 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pSB700 lenti gRNA Cerulean-zhang2.0 Weiss full

   Plasmid

   #79383

   Purpose

   Cas9 gRNA variant to recruit ms2 proteins

   Depositor

   George Church

   Article

   Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

   Insert

   Zhang 2.0 + scaffold
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Feb. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. STAGR_SAMScaffold_hU6

   Plasmid

   #102843

   Purpose

   Can be used as PCR template for a STAgR reaction

   Depositor

   Stefan Stricker

   Article

   Breunig et al PLoS One. 2018 Apr 27;13(4):e0196015. do

   Insert

   gRNAScaffold_hU6promoter
   

   Use

   As pcr template

   Available Since

   Jan. 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pLKO mouse shRNA 2 raptor

   Plasmid

   #21340

   Depositor

   David Sabatini

   Article

   Peterson et al Cell. 2009 May 29;137(5):873-86. doi: 10

   Insert

   raptor shRNA 2 (Rptor Mouse)
   

   Use

   Lentiviral and RNAi

   Expression

   Mammalian

   Available Since

   July 6, 2009

   Availability

   Academic Institutions and Nonprofits only

10. STAgR_tdTomato

    Plasmid

    #102993

    Purpose

    Can be used as backbone for a STAgR reaction

    Depositor

    Stefan Stricker

    Article

    Breunig et al PLoS One. 2018 Apr 27;13(4):e0196015. do

    Type

    Empty backbone

    Use

    CRISPR; Pcr template for stagr vectors

    Promoter

    U6

    Available Since

    March 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pENTR221-NFAT5

    Plasmid

    #79506

    Purpose

    MultiSite Gateway entry clones, second fragment (attL5, attL2) Human NFAT5 CDS

    Depositor

    Peter Jon Nelson

    Article

    Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

    Insert

    NFAT5 (NFAT5 Human)
    

    Use

    Multisite gateway entry clone

    Promoter

    no Promoter

    Available Since

    May 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pU6_(GLuc)_INT[3xPP7_SL]

    Plasmid

    #68424

    Purpose

    Transient expression of an "INT" construct_bearing three PP7 Stem-loops, targeting the GLuc reporter, in mammalian cells. U6 promoter

    Depositor

    John Rinn

    Article

    Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

    Insert

    INT construct bearing three PP7 Stem-loops
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Mammalian

    Promoter

    human U6

    Available Since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. FLAG-Ago1

    Plasmid

    #21533

    Depositor

    Edward Chan

    Article

    Lian et al RNA. 2009 May . 15(5):804-13.

    Insert

    Ago1 (AGO1 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Available Since

    July 13, 2009

    Availability

    Academic Institutions and Nonprofits only

14. RCAS-myrAkt

    Plasmid

    #11547

    Depositor

    Sandra Orsulic

    Article

    Orsulic et al Cancer Cell. 2002 Feb . 1(1):53-62.

    Insert

    Protein Kinase B (Akt1 Mouse)
    

    Use

    Retroviral

    Tags

    HA tag and myr signal

    Available Since

    April 5, 2006

    Availability

    Academic Institutions and Nonprofits only

15. pAAV-SMVP-Cas9N-U6-gRNA TdR

    Plasmid

    #80940

    Purpose

    Expresses Cas9N in mammalian cells; expresses gRNA TdR for Ai9 cleavage.

    Depositor

    George Church

    Article

    Chew et al Nat Methods. 2016 Sep 5. doi: 10.1038/nm

    Insert

    Cas9N
    

    Use

    AAV, CRISPR, and Synthetic Biology

    Expression

    Mammalian

    Available Since

    Oct. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. Orange Nano-lantern(Ca2+)/pcDNA3

    Plasmid

    #71346

    Purpose

    Express Orange Nano-lantern-based luminescent Ca2+ indicator in mammalian cell

    Depositor

    Takeharu Nagai

    Article

    Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

    Insert

    Orange Nano-lantern(Ca2+)
    

    Expression

    Mammalian

    Available Since

    Dec. 8, 2015

    Availability

    Academic Institutions and Nonprofits only

17. pActinin-miRFP703

    Plasmid

    #79992

    Purpose

    Alpha actinin labeling with near-infrared fluorescent protein miRFP703

    Depositor

    Vladislav Verkhusha

    Article

    Shcherbakova et al Nat Commun. 2016 Aug 19;7:12405. doi: 10

    Insert

    Alpha-Actinin (ACTN1 Human)
    

    Tags

    miRFP703

    Expression

    Mammalian

    Promoter

    c

    Available Since

    July 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pMX-Flag-WIP

    Plasmid

    #11772

    Depositor

    Jack Strominger

    Article

    Krzewski et al J Cell Biol. 2006 Apr 10. 173(1):121-32.

    Insert

    WIP (WIPF1 Human)
    

    Use

    Retroviral

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    Feb. 15, 2007

    Availability

    Academic Institutions and Nonprofits only

19. pHis-zfEpoa

    Plasmid

    #65611

    Purpose

    Express soluble His-tagged zebrafish erythropoietin in E. coli

    Depositor

    Petr Bartunek

    Article

    Stachura et al Blood. 2009 Jul 9;114(2):279-89. doi: 10

    Insert

    erythropoietin (epoa Zebrafish)
    

    Tags

    (His)6

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Orange Nano-lantern/pcDNA3

    Plasmid

    #67661

    Purpose

    orange luminescent protein for high-speed single-cell and whole body imaging

    Depositor

    Takeharu Nagai

    Article

    Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

    Insert

    Orange Nano-lantern
    

    Expression

    Mammalian

    Available Since

    Sept. 10, 2015

    Availability

    Academic Institutions and Nonprofits only