We narrowed to 19,003 results for: Tec

1. CEBPalpha NGFR

   Plasmid

   #44627

   Depositor

   Ellen Rothenberg

   Article

   Del Real et al Development. 2013 Mar;140(6):1207-19. do

   Insert

   CEBPalpha (Cebpa Mouse)
   

   Use

   Retroviral

   Tags

   IRES and truncated NGFR

   Expression

   Mammalian

   Available Since

   April 30, 2013

   Availability

   Academic Institutions and Nonprofits only

2. pCS2-Flag-TTGZFP-FokI-DD

   Plasmid

   #18755

   Depositor

   Scot Wolfe

   Article

   Meng et al Nat Biotechnol. 2008 Jun . 26(6):695-701

   Insert

   zinc finger nuclease
   

   Expression

   Bacterial

   Mutation

   RNA expression vector for expressing the zinc fin…

   Available Since

   July 11, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pSI-359

   Plasmid

   #131131

   Purpose

   gRNA expression vector for A-to-G base editing reporter

   Depositor

   Nozomu Yachie

   Article

   Sakata et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

   Insert

   A-to-G reporter activation gRNA
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   Human U6 promoter

   Available Since

   Sept. 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. L2-AA007

   Plasmid

   #185750

   Purpose

   Vector to introduce HygR and eGFP under control of p35S into the genome of the hornwort Anthoceros agrestis via Agrobacterium mediated transformation. eGFP contains a cell membrane localization tag.

   Depositor

   Peter Szoevenyi

   Article

   Frangedakis et al New Phytol. 2021 Jun 2. doi: 10.1111/nph

   Inserts

   HygR2
   eGFP
   

   Tags

   membrane localization tag Lti6b

   Expression

   Plant

   Available Since

   July 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pNXgate32-3HA

   Plasmid

   #105076

   Purpose

   Yeast mating-based Split Ubiquitin Assay, prey vector for in vivo cloning

   Depositor

   Wolf Frommer

   Article

   Obrdlik et al Proc Natl Acad Sci U S A. 2004 Aug 17;10

   Type

   Empty backbone

   Tags

   3xHA and NubG

   Expression

   Yeast

   Available Since

   Feb. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pAPcrRNA_Lmo

   Plasmid

   #126501

   Purpose

   L.monocytogenes CRISPR Type I-B crRNA cloning vector for mammalian expression driven by U6 promoter

   Depositor

   Charles Gersbach

   Article

   Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Sept. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pOCC61

   Plasmid

   #118877

   Purpose

   shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with C-terminal monomeric GFP and C-terminal HIS6 tag, cleavable with 3C

   Depositor

   Marc Bickle, PEPC MPI CBG

   Article

   Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

   Insert

   NcoI-NotI-ccdB-AscI-mGFP-3C-HIS6-stop-HindIII cassette
   

   Tags

   mGFP, HIS6, cleavable with 3C protease

   Expression

   Insect

   Promoter

   polH

   Available Since

   Jan. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. EK0423 SFFV-mTagBFP2-SGc(s70587)cSG-mCherry (FLP-IN)

   Plasmid

   #191162

   Purpose

   Expression of a sensor for synthetic sequence 1 (EK0208) in mammalian cells; mTagBFP2 marker, mCherry output

   Depositor

   Xiaojing Gao

   Article

   Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

   Insert

   mTagBFP:s70587:mCherry
   

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   SFFV

   Available Since

   Oct. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pOCC28

   Plasmid

   #118873

   Purpose

   shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal MBP tag, cleavable with 3C, and C-terminal HIS6 tag, cleavable with 3C

   Depositor

   Marc Bickle, PEPC MPI CBG

   Article

   Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

   Insert

   NcoI-MBP-3C--NotI-ccdB-AscI-3C-HIS6-stop-HindIII cassette
   

   Tags

   HIS6, cleavable with 3C protease and MBP, cleavab…

   Expression

   Insect

   Promoter

   polH

   Available Since

   Jan. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pGEL033

    Plasmid

    #137902

    Purpose

    STU2.0 rAPOBEC1-SpyCas9(D10A) for plant genome base editing

    Depositor

    Yong Zhang

    Article

    Tang et al Plant Biotechnol J. 2019 Jul;17(7):1431-

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Plant

    Available Since

    Feb. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. AA345

    Plasmid

    #216031

    Purpose

    Fragmid fragment: (Cas protein) Cas13d

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Has Service

    Cloning Grade DNA

    Insert

    Cas13d_v1.1 [Dj]
    

    Use

    CRISPR; Fragment

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pRDA_834

    Plasmid

    #216087

    Purpose

    Cas9 [Sp] knockout targeting CD59, positive control

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Insert

    CD59 guide
    

    Use

    CRISPR and Lentiviral; Positive controls

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. SNF-5 pXOOM

    Plasmid

    #48697

    Purpose

    Expressed SNF-5 in Xenopus oocytes or mammalian cells

    Depositor

    Dmitri Boudko

    Article

    Metzler et al J Exp Biol. 2013 Aug 1;216(Pt 15):2843-5

    Insert

    snf-5 (snf-5 Nematode)
    

    Use

    Xenopus oocyte expression

    Expression

    Mammalian

    Mutation

    K42E (when compared to NP_496735.1)

    Promoter

    T7

    Available Since

    Dec. 20, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pX459-sg-mGreenLantern/EGFP

    Plasmid

    #179913

    Purpose

    All in one Cas9 plasmid with puromycin resistance and a single guide RNA targeting mGreenLantern and EGFP fluorescent proteins.

    Depositor

    Indeever Madireddy, Johan Sosa

    Article

    Madireddy et al MicroPubl Biol. 2022 Jun 16;2022. doi: 1

    Insert

    mGreenLantern/EGFP sgRNA
    

    Use

    CRISPR

    Tags

    2A-Puro and 3XFLAG

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    April 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCI-globin_del5UTR_WT-xrRNA-4H

    Plasmid

    #108366

    Purpose

    Expresses wild type beta-globin reporter; enables detection of 5'-3' decay intermediates (xrFrag) and allows detection via northern blot (4H binding sites)

    Depositor

    Niels Gehring

    Article

    Boehm et al Nat Commun. 2016 Dec 5;7:13691. doi: 10.

    Insert

    Wild type beta-globin reporter with MVE xrRNA and 4H probe binding sites
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pGEL029

    Plasmid

    #137898

    Purpose

    STU2.0 SpyCas9 RZ system for plant genome editing

    Depositor

    Yong Zhang

    Article

    Tang et al Plant Biotechnol J. 2019 Jul;17(7):1431-

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Plant

    Available Since

    Feb. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pTS2394-Tier1-PhCMV-3xNLS_mTagBFP2

    Plasmid

    #169531

    Purpose

    Tier-1 vector encoding PhCMV-driven blue fluorescent protein mTagBFP2 with nuclear localization (PhCMV-3xNLS-mTagBFP2-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PCMV-driven nucleus-localized monomeric Tag blue fluorescent protein 2
    

    Expression

    Mammalian

    Promoter

    PhCMV

    Available Since

    June 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. shNTC

    Plasmid

    #73549

    Purpose

    Non-targeting control shRNA cloned in retroviral vector pMLP (MSCV-based vector expressing shRNA in a mir30 context).

    Depositor

    Gerardo Ferbeyre

    Article

    Acevedo et al Cancer Res. 2016 Jun 1;76(11):3252-64. d

    Insert

    shNTC
    

    Use

    Retroviral

    Expression

    Mammalian

    Promoter

    LTR

    Available Since

    Aug. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

19. pcDNA3.1(+)-sapphireAKAR-NLS

    Plasmid

    #118467

    Purpose

    cp-T-sapphire-based single-color PKA biosensor targeted to the nucleus.

    Depositor

    Jin Zhang

    Article

    Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

    Insert

    sapphireAKAR-NLS
    

    Tags

    Nuclear localization signal (NLS)

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pT2SCb

    Plasmid

    #59385

    Purpose

    Template plasmid for amplification of a selection cassette that includes two transcription terminator elements, an I-SceI site and a modified chloramphenicol resistance gene.

    Depositor

    Shelley Copley

    Article

    Kim et al BMC Biotechnol. 2014 Sep 25;14(1):84.

    Insert

    TT-ISceI-cat2
    

    Use

    Template

    Mutation

    The 3’-end of the chloramphenicol resistance gene…

    Available Since

    Sept. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge