We narrowed to 9,182 results for: mel

1. pGG438

   Plasmid

   #165486

   Purpose

   Vector for expression of the SpCas9 VRKG variant in human cells: CMV-T7-humanVRKG(SpCas9, D1135V/S1136R/D1332K/R1333G)-1xNLS(SV40)-3xFLAG

   Depositor

   Marcus Noyes

   Article

   Goldberg et al Nat Commun. 2021 Jan 13;12(1):349. doi:

   Insert

   Mammalian codon-optimized Streptococcus pyogenes Cas9 VRKG(D1135V/S1136R/D1332K/R1333G)-1xNLS(SV40)-3xFlag
   

   Use

   CRISPR

   Tags

   3x FLAG and SV40 NLS

   Expression

   Mammalian

   Mutation

   D1135V, S1136R, D1332K, and R1333G mutations in S…

   Promoter

   CMV and T7

   Available Since

   April 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. INCTbiosyn-pUB-HspINT4

   Plasmid

   #127513

   Purpose

   Plasmid encodes H. sapiens codon optimized Integrase 4.

   Depositor

   Martin Bonamino

   Article

   Gomide et al Commun Biol. 2020 May 22;3(1):255. doi:

   Insert

   Integrase 4 coding sequence codon optimized for H. sapiens expression.
   

   Expression

   Mammalian

   Available Since

   May 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. INCTbiosyn-pUB-HspINT5

   Plasmid

   #127514

   Purpose

   Plasmid encodes H. sapiens codon optimized Integrase 5.

   Depositor

   Martin Bonamino

   Article

   Gomide et al Commun Biol. 2020 May 22;3(1):255. doi:

   Insert

   Integrase 5 coding sequence codon optimized for H. sapiens expression.
   

   Expression

   Mammalian

   Available Since

   May 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBADHISTEV_PfeEH258A

   Plasmid

   #129122

   Purpose

   expresses H258A mutant of PfeE in E.coli

   Depositor

   James Naismith

   Article

   Perraud et al ACS Chem Biol. 2018 Sep 21;13(9):2603-26

   Insert

   Periplasmic Esterase PfeE (PA2689 Pseudomonas aeruginosa PAO1)
   

   Tags

   TEV protease cleavable 6xHis

   Expression

   Bacterial

   Mutation

   Signal peptide sequence has been removed, mutat…

   Promoter

   PBAD

   Available Since

   Aug. 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBADHISTEV_PfeES157A

   Plasmid

   #129121

   Purpose

   expresses S157A mutant of PfeE in E.coli

   Depositor

   James Naismith

   Article

   Perraud et al ACS Chem Biol. 2018 Sep 21;13(9):2603-26

   Insert

   Periplasmic Esterase PfeE (PA2689 Pseudomonas aeruginosa PAO1)
   

   Tags

   TEV protease cleavable 6xHis

   Expression

   Bacterial

   Mutation

   Signal peptide sequence has been removed, mutat…

   Promoter

   PBAD

   Available Since

   Aug. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. Designed HEL-binding scFv D44.1des

   Plasmid

   #111719

   Purpose

   Yeast Surface Display of D44.1des

   Depositor

   Sarel Fleishman

   Article

   Warszawski et al PLoS Comput Biol. 2019 Aug 23;15(8):e100

   Insert

   D44.1 des
   

   Tags

   cmyc tag

   Expression

   Yeast

   Mutation

   L Q89G, L S55V, L S43P, H V37M, H H43G, H W47Y, H…

   Available Since

   July 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. Dscam1_7.27.26 EC10-Fc-His

   Plasmid

   #72189

   Purpose

   Expresses the 10 N-terminal extracellular domains of the Dscam, isoform 7.27.26 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

   Depositor

   Woj Wojtowicz

   Article

   Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

   Insert

   Dscam1_7.27.26
   

   Tags

   Fc-His

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. Dscam1_7.27.23 EC10-Fc-His

   Plasmid

   #72187

   Purpose

   Expresses the 10 N-terminal extracellular domains of the Dscam, isoform 7.27.23 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

   Depositor

   Woj Wojtowicz

   Article

   Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

   Insert

   Dscam1_7.27.23
   

   Tags

   Fc-His

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. Dscam1_7.27.26 EC10-AP-His

   Plasmid

   #72063

   Purpose

   Expresses the 10 N-terminal extracellular domains of the Dscam, isoform 7.27.26 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

   Depositor

   Woj Wojtowicz

   Article

   Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

   Insert

   Dscam1_7.27.26
   

   Tags

   AP-His

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. GAL-ARF

    Plasmid

    #89121

    Purpose

    mammalian expression of p14-hARF linked to GAL4 DNA binding domain

    Depositor

    Elizabeth Wilson

    Article

    Minges et al J Biol Chem. 2015 Oct 9;290(41):25174-87

    Insert

    p14-hARF (CDKN2A Human)
    

    Tags

    GAL4 DBD

    Expression

    Mammalian

    Available Since

    April 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. Dscam1_7.27.30 EC10-AP-His

    Plasmid

    #72064

    Purpose

    Expresses the 10 N-terminal extracellular domains of the Dscam, isoform 7.27.30 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Dscam1_7.27.30
    

    Tags

    AP-His

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. Dscam1_7.27.22 EC10-AP-His

    Plasmid

    #72060

    Purpose

    Expresses the 10 N-terminal extracellular domains of the Dscam, isoform 7.27.22 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Dscam1_7.27.22
    

    Tags

    AP-His

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. Dscam1_7.27.23 EC10-AP-His

    Plasmid

    #72061

    Purpose

    Expresses the 10 N-terminal extracellular domains of the Dscam, isoform 7.27.23 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Dscam1_7.27.23
    

    Tags

    AP-His

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. Dscam1_7.27.22 EC10-Fc-His

    Plasmid

    #72186

    Purpose

    Expresses the 10 N-terminal extracellular domains of the Dscam, isoform 7.27.22 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Dscam1_7.27.22
    

    Tags

    Fc-His

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pUAST-HA, Pbl-A DH domain

    Plasmid

    #69794

    Purpose

    Pebble isoform A, DH domain, in P element-based pUAST vector for Gal4-regulated expression in Drosophila.

    Depositor

    Arno Muller

    Article

    van Impel et al Development. 2009 Mar;136(5):813-22. doi

    Insert

    Pebble DH domain
    

    Use

    P element-based puast vector for gal4-regulated e…

    Tags

    HA-Tag

    Expression

    Insect

    Mutation

    Contains only the Pebble DH domain

    Promoter

    hsp70

    Available Since

    Oct. 7, 2015

    Availability

    Academic Institutions and Nonprofits only

16. pCDF1-ADAMTS18 (P1035S)

    Plasmid

    #29558

    Depositor

    Yardena Samuels

    Article

    Wei et al Mol Cancer Res. 2010 Nov . 8(11):1513-25

    Insert

    ADAMTS18 (ADAMTS18 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    changed proline 1035 to serine

    Available Since

    Sept. 7, 2011

    Availability

    Academic Institutions and Nonprofits only

17. pCDF1-ADAM29 (S112F)

    Plasmid

    #31145

    Depositor

    Yardena Samuels

    Article

    Wei et al Hum Mutat. 2011 Jun;32(6):E2148-75. doi:

    Insert

    ADAM29 (ADAM29 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Serine 112 changed to Pheylalnine

    Available Since

    Sept. 1, 2011

    Availability

    Academic Institutions and Nonprofits only

18. pcDNA3.1-ADAM7 (S703N)

    Plasmid

    #31141

    Depositor

    Yardena Samuels

    Article

    Wei et al Hum Mutat. 2011 Jun;32(6):E2148-75. doi:

    Insert

    ADAM7 (ADAM7 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    S703N

    Available Since

    July 22, 2011

    Availability

    Academic Institutions and Nonprofits only

19. pCDF1-ADAMTS18 (C638S)

    Plasmid

    #29555

    Depositor

    Yardena Samuels

    Article

    Wei et al Mol Cancer Res. 2010 Nov . 8(11):1513-25

    Insert

    ADAMTS18 (ADAMTS18 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    changed cysteine 638 to serine

    Available Since

    July 22, 2011

    Availability

    Academic Institutions and Nonprofits only

20. pET28a-MED20

    Plasmid

    #15435

    Depositor

    Joan Conaway, Ronald Conaway

    Article

    Sato et al J Biol Chem. 2003 Apr 25. 278(17):15123-

    Insert

    MED20 (Med20 Mouse)
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    N/A

    Available Since

    Aug. 10, 2007

    Availability

    Academic Institutions and Nonprofits only