We narrowed to 9,236 results for: mel

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pCS2+8-CYP2A6(C14A)-flag-tev-halo-his

Plasmid

#227159

Purpose

For T-REX experiments of CYP2A6 C14A mutant. A reactive electrophilic species (RES) Sensor.

Depositor

Yimon Aye

Article

Liu et al Cell. 2024 Dec 26;187(26):7450-7469.e29.

Insert

CYP2A6 (CYP2A6 Human)

Tags

Flag, HaloTag, and His6

Expression

Mammalian

Mutation

changed cysteine 14 to alanine

Promoter

CMV and SP6

Available Since

Aug. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pCS2+8-CYP2A6(C82A)-flag-tev-halo-his

Plasmid

#227160

Purpose

For T-REX experiments of CYP2A6 C82A mutant. Less sensing ability to reactive electrophilic species (RES).

Depositor

Yimon Aye

Article

Liu et al Cell. 2024 Dec 26;187(26):7450-7469.e29.

Insert

CYP2A6 (CYP2A6 Human)

Tags

Flag, HaloTag, and His6

Expression

Mammalian

Mutation

changed cysteine 82 to alanine

Promoter

CMV and SP6

Available Since

Aug. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pCS2+8-CYP2A6(C439A)-flag-tev-his

Plasmid

#227167

Purpose

For overexpression of CYP2A6 C439A mutant in mammalian cells and purification.

Depositor

Yimon Aye

Article

Liu et al Cell. 2024 Dec 26;187(26):7450-7469.e29.

Insert

CYP2A6 (CYP2A6 Human)

Tags

Flag and His6

Expression

Mammalian

Mutation

changed cysteine 439 to alanine

Promoter

CMV and SP6

Available Since

Aug. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pCS2+8-cyp-33e1(C295A)-flag-tev-halo-his

Plasmid

#227156

Purpose

For T-REX experiments of cyp-33e1 C295A mutant. Less sensing ability to reactive electrophilic species (RES).

Depositor

Yimon Aye

Article

Liu et al Cell. 2024 Dec 26;187(26):7450-7469.e29.

Insert

cyp-33e1 (cyp-33E1 Nematode)

Tags

Flag, HaloTag, and His6

Expression

Mammalian

Mutation

changed cysteine 295 to alanine

Promoter

CMV and SP6

Available Since

Aug. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pCS2+8-cyp-33e1(C439A)-flag-tev-halo-his

Plasmid

#227157

Purpose

For T-REX experiments of cyp-33e1 C439A mutant. Less sensing ability to reactive electrophilic species (RES).

Depositor

Yimon Aye

Article

Liu et al Cell. 2024 Dec 26;187(26):7450-7469.e29.

Insert

cyp-33e1 (cyp-33E1 Nematode)

Tags

Flag, HaloTag, and His6

Expression

Mammalian

Mutation

changed cysteine 439 to alanine

Promoter

CMV and SP6

Available Since

Aug. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pCS2+ Zf nhsl1b-mNeongreen

Plasmid

#233883

Purpose

mNeongreen tagged form of zebrafish nhsl1b. For in vitro transcription (SP6) or expression through CMV.

Depositor

Nicolas David

Article

Escot et al Commun Biol. 2025 Feb 28;8(1):328. doi:

Insert

nhsl1b (nhsl1b Zebrafish)

Use

In vitro synthesis of mrna

Tags

mNeongreen

Expression

Mammalian

Available Since

July 18, 2025

Availability

Academic Institutions and Nonprofits only

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pMT-sfGFP-TurboID-ER

Plasmid

#240230

Purpose

Expression of ER-localized sfGFP-TurboID under control of copper-inducible promoter. Can be used to generate stable cell lines by Hygromycin selection.

Depositor

Justin Bosch, Norbert Perrimon

Article

Bosch et al bioRxiv 2025.07.09.659702; doi: https://

Insert

BIP-sfGFP-TurboID-KDEL

Expression

Insect

Promoter

MT

Available Since

July 11, 2025

Availability

Academic Institutions and Nonprofits only

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pMT-sfGFP-TurboID

Plasmid

#240231

Purpose

Expression of sfGFP-TurboID under control of copper-inducible promoter. Can be used to generate stable cell lines by Hygromycin selection.

Depositor

Justin Bosch, Norbert Perrimon

Article

Bosch et al bioRxiv 2025.07.09.659702; doi: https://

Insert

sfGFP-TurboID

Expression

Insect

Promoter

MT

Available Since

July 11, 2025

Availability

Academic Institutions and Nonprofits only

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pMT-Myc-BirA*G3-ER

Plasmid

#240234

Purpose

Expression of ER-localized Myc-BirA*-G3 under control of copper-inducible promoter. Can be used to generate stable cell lines by Hygromycin selection.

Depositor

Justin Bosch, Norbert Perrimon

Article

Bosch et al bioRxiv 2025.07.09.659702; doi: https://

Insert

BiP-Myc-BirA*G3-KDEL

Expression

Insect

Promoter

MT

Available Since

July 11, 2025

Availability

Academic Institutions and Nonprofits only

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pWal10-roe-sfGFP

Plasmid

#240239

Purpose

Gateway destination plasmid to generate C-terminal sfGFP fusions under control of UAS promoter. Can be used to generate transgenic fly lines by white+ fly eye selection and phiC31 integration.

Depositor

Justin Bosch, Norbert Perrimon

Article

Bosch et al bioRxiv 2025.07.09.659702; doi: https://

Type

Empty backbone

Use

Gateway destination vector

Expression

Insect

Available Since

July 11, 2025

Availability

Academic Institutions and Nonprofits only

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AAV-DIO-mPOMC

Plasmid

#239042

Purpose

Overexpression of POMC gene

Depositor

Qingchun Tong

Article

Xu et al Cell Rep. 2023 May 30;42(5):112502. doi:

Insert

mPOMC (Pomc Mouse)

Use

AAV

Promoter

CAG

Available Since

May 21, 2025

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR-v1-sgDHFR

Plasmid

#233898

Purpose

lentiviral vector expressing Cas9 and an sgRNA targeting DHFR

Depositor

Jason Cantor

Article

Flickinger et al Nat Metab. 2025 May 2. doi: 10.1038/s422

Insert

sgRNA targeting DHFR (DHFR Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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pTBL3331 MTK234-TetO-AtoBpegRNA

Plasmid

#226668

Purpose

MTK234 part containing tet-inducible variant of the hU6 promoter driving an A to B pegRNA

Depositor

Chang Liu

Article

Loveless et al Nat Chem Biol. 2025 Apr;21(4):512-521. d

Insert

hU6-TetO-AtoBpegRNA

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Promoter

hU6-TetO

Available Since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pTBL3346 PB-nicking guide-blast

Plasmid

#226665

Purpose

PiggyBac cargo vector encoding protective nicking sgRNA marked with blasticidin

Depositor

Chang Liu

Article

Loveless et al Nat Chem Biol. 2025 Apr;21(4):512-521. d

Insert

protective nicking sgRNA

Use

CRISPR

Expression

Mammalian

Promoter

hU6

Available Since

Oct. 28, 2024

Availability

Academic Institutions and Nonprofits only

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PiggyBac EF1a-eGFP-Puro U6 MMP2 g2

Plasmid

#170825

Purpose

PiggyBac Cas13d sgRNA plasmid for MMP2 knockdown

Depositor

Xiaoping Bao

Article

Chang et al Cell Rep. 2022 Jul 19;40(3):111128. doi:

Insert

Cas13d MMP2 gRNA2

Use

Piggybac transposon

Expression

Mammalian

Promoter

U6

Available Since

July 19, 2024

Availability

Academic Institutions and Nonprofits only

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PiggyBac EF1a-eGFP-Puro U6 ANXA2 g1

Plasmid

#170826

Purpose

PiggyBac Cas13d sgRNA plasmid for ANXA2 knockdown

Depositor

Xiaoping Bao

Article

Chang et al Cell Rep. 2022 Jul 19;40(3):111128. doi:

Insert

Cas13d ANXA2 gRNA1

Use

Piggybac transposon

Expression

Mammalian

Promoter

U6

Available Since

July 19, 2024

Availability

Academic Institutions and Nonprofits only

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PiggyBac EF1a-eGFP-Puro U6 ANXA2 g2

Plasmid

#170827

Purpose

PiggyBac Cas13d sgRNA plasmid for ANXA2 knockdown

Depositor

Xiaoping Bao

Article

Chang et al Cell Rep. 2022 Jul 19;40(3):111128. doi:

Insert

Cas13d ANXA2 gRNA2

Use

Piggybac transposon

Expression

Mammalian

Promoter

U6

Available Since

July 19, 2024

Availability

Academic Institutions and Nonprofits only

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PiggyBac EF1a-eGFP-Puro U6 CLCN3 g1

Plasmid

#170828

Purpose

PiggyBac Cas13d sgRNA plasmid for CLCN3 knockdown

Depositor

Xiaoping Bao

Article

Chang et al Cell Rep. 2022 Jul 19;40(3):111128. doi:

Insert

Cas13d CLCN3 gRNA1

Use

Piggybac transposon

Expression

Mammalian

Promoter

U6

Available Since

July 19, 2024

Availability

Academic Institutions and Nonprofits only

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PiggyBac EF1a-eGFP-Puro U6 CLCN3 g2

Plasmid

#170829

Purpose

PiggyBac Cas13d sgRNA plasmid for CLCN3 knockdown

Depositor

Xiaoping Bao

Article

Chang et al Cell Rep. 2022 Jul 19;40(3):111128. doi:

Insert

Cas13d CLCN3 gRNA2

Use

Piggybac transposon

Expression

Mammalian

Promoter

U6

Available Since

July 19, 2024

Availability

Academic Institutions and Nonprofits only

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pBS-KDRT-STOP-loxP-3XP3::dsRed-loxP-STOP-KDRT-1XALFA

Plasmid

#217509

Purpose

KD Recombinase dependent conditional 1X ALFA cassette

Depositor

S. Lawrence Zipursky

Article

Sanfilippo et al Neuron. 2024 Jan 13:S0896-6273(23)00973-

Insert

KDRT-STOP-loxP-3XP3::dsRed-loxP-STOP-KDRT-1XALFA

Tags

ALFA tag

Expression

Insect

Available Since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

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