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pLL3.7m-psMEK2

Plasmid

#89363

Purpose

A lenti-viral vector expressing photoswitchable MEK2 in mammalian cells

Depositor

Michael Lin

Article

Zhou et al Science. 2017 Feb 24;355(6327):836-842.

Insert

psMEK2 (MAP2K2 Synthetic, Human)

Use

Lentiviral

Tags

Human influenza hemagglutinin (HA) tag and pdDron…

Expression

Mammalian

Promoter

CMV

Available Since

April 10, 2017

Availability

Academic Institutions and Nonprofits only

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pC014 - LwCas13a-msfGFP

Plasmid

#91902

Purpose

Expresses active LwCas13a for mammalian RNA targeting

Depositor

Feng Zhang

Article

Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

Insert

LwCas13a

Use

CRISPR

Tags

NLS and msfGFP-NLS-3xHA

Expression

Mammalian

Promoter

EF1a

Available Since

Dec. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pLL3.7m-psCDK5

Plasmid

#89365

Purpose

A lenti-viral vector expressing photoswitchable CDK5 in mammalian cells

Depositor

Michael Lin

Article

Zhou et al Science. 2017 Feb 24;355(6327):836-842.

Insert

psCDK5 (CDK5 Synthetic, Human)

Use

Lentiviral

Tags

Human influenza hemagglutinin (HA) tag and pdDron…

Expression

Mammalian

Promoter

CMV

Available Since

April 10, 2017

Availability

Academic Institutions and Nonprofits only

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pXR002: EF1a-dCasRx-2A-EGFP

Plasmid

#109050

Purpose

Catalytically inactive dCasRx with 2A-EGFP

Depositor

Patrick Hsu

Article

Konermann et al Cell. 2018 Mar 8. pii: S0092-8674(18)302

Insert

dCasRx

Use

CRISPR and Lentiviral

Tags

2A-eGFP, HA, and NLS

Expression

Mammalian

Mutation

R239A/H244A/R858A/H863A

Available Since

April 26, 2018

Availability

Academic Institutions and Nonprofits only

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dCas13d-BFP

Plasmid

#215720

Purpose

Catalytically inactive dCasRx with 2A-BFP

Depositor

Chaolin Zhang

Article

Recinos et al Nat Commun. 2024 May 7;15(1):3839. doi:

Insert

dCasRx

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

May 30, 2024

Availability

Academic Institutions and Nonprofits only

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pFUGW mCherry-KASH

Plasmid

#131505

Purpose

mCherry_KASH epression under hUBC promotor; serves as backbone for ORANGE lenti knock-in

Depositor

Harold MacGillavry

Article

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Insert

mCherry_KASH

Use

Lentiviral

Expression

Mammalian

Promoter

hUBC

Available Since

Sept. 20, 2019

Availability

Academic Institutions and Nonprofits only

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Reporter-1

Plasmid

#180218

Purpose

Surrogate reporter plasmid for the assay of targeted RNA editing

Depositor

Wensheng Wei

Article

Qu et al Nat Biotechnol. 2019 Sep;37(9):1059-1069

Insert

Surrogate reporter

Use

Lentiviral

Available Since

April 28, 2022

Availability

Academic Institutions and Nonprofits only

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Reporter-2

Plasmid

#180219

Purpose

Surrogate reporter plasmid for the assay of targeted RNA editing

Depositor

Wensheng Wei

Article

Qu et al Nat Biotechnol. 2019 Sep;37(9):1059-1069

Insert

Surrogate reporter

Use

Lentiviral

Available Since

April 28, 2022

Availability

Academic Institutions and Nonprofits only

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pLL3.7m-psMEK2

Plasmid

#89363

Purpose

A lenti-viral vector expressing photoswitchable MEK2 in mammalian cells

Depositor

Michael Lin

Article

Zhou et al Science. 2017 Feb 24;355(6327):836-842.

Insert

psMEK2 (MAP2K2 Synthetic, Human)

Use

Lentiviral

Tags

Human influenza hemagglutinin (HA) tag and pdDron…

Expression

Mammalian

Promoter

CMV

Available Since

April 10, 2017

Availability

Academic Institutions and Nonprofits only

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pXR001: EF1a-CasRx-2A-EGFP

Plasmid

#109049

Purpose

CasRx (NLS-RfxCas13d-NLS) with 2A-EGFP for RNA targeting

Depositor

Patrick Hsu

Article

Konermann et al Cell. 2018 Mar 8. pii: S0092-8674(18)302

Insert

CasRx

Use

CRISPR and Lentiviral

Tags

2A-eGFP, HA, and NLS

Expression

Mammalian

Promoter

EF1A

Available Since

May 29, 2018

Availability

Academic Institutions and Nonprofits only

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pLL3.7m-psRaf1

Plasmid

#89364

Purpose

A lenti-viral vector expressing photoswitchable Raf1 in mammalian cells

Depositor

Michael Lin

Article

Zhou et al Science. 2017 Feb 24;355(6327):836-842.

Insert

psRaf1 (RAF1 Synthetic, Human)

Use

Lentiviral

Tags

Human influenza hemagglutinin (HA) tag and pdDron…

Expression

Mammalian

Promoter

CMV

Available Since

April 10, 2017

Availability

Academic Institutions and Nonprofits only

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dCAS9-VP64_GFP

Plasmid

#61422

Purpose

Expresses dCAS9-VP64 activator with 2A GFP

Depositor

Feng Zhang

Article

Konermann et al Nature. 2014 Dec 10. doi: 10.1038/nature

Has Service

Concentrated Lentiviral Prep

Insert

dCAS9(D10A,H840A)-VP64_2A_GFP

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

D10A and H840A in Cas9

Promoter

EF1A

Available Since

Dec. 15, 2014

Availability

Academic Institutions and Nonprofits only

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pC035 - dLwCas13a-msfGFP

Plasmid

#91925

Purpose

Expresses catalytically inactive LwCas13a for mammalian RNA targeting

Depositor

Feng Zhang

Article

Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

Insert

dLwCas13a

Use

CRISPR

Tags

NLS-3xHA and NLS-msfGFP

Expression

Mammalian

Mutation

R488A and R1060A

Promoter

EF-1a

Available Since

Nov. 16, 2018

Availability

Academic Institutions and Nonprofits only

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pC0056 - LwCas13a-msfGFP-NES

Plasmid

#105815

Purpose

Expresses active LwaCas13a-NES for mammalian RNA targeting

Depositor

Feng Zhang

Article

Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

Insert

LwCas13a

Use

CRISPR and Lentiviral

Tags

NES and msfGFP-NES-3xHA

Expression

Mammalian

Promoter

EF-1a

Available Since

Feb. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pMuLE_EXPR_CMV-eGFP_TOP-iRFP_PTCH1-mTurquoise2_CBF-tdTomato

Plasmid

#113865

Purpose

Triple pathway reporter, 3P-Fluor; wnt-iRFP, hedgehog-mTurquoise2, notch-tdTomato; plus CMV-eGFP. Gateway expression vector for lentivirus generation.

Depositor

Manfred Ogris

Article

Maier et al PLoS One. 2019 Dec 20;14(12):e0226570. d

Inserts

CMV-eGFP
TOP-iRFP
PTCH1-mTurquoise2
CBF-tdTomato

Use

Lentiviral

Expression

Mammalian

Available Since

Jan. 22, 2020

Availability

Academic Institutions and Nonprofits only

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PLX306 iCre tetO tomato rtTA

Plasmid

#158050

Purpose

lenti-viral construct with Cre recombinase and doxycycline inducible expression of tdTomato

Depositor

Daniel Schramek

Article

Loganathan et al Science. 2020 Mar 13;367(6483):1264-1269

Insert

tdTomato

Tags

none

Expression

Mammalian

Promoter

tetO promoter

Available Since

Oct. 5, 2020

Availability

Academic Institutions and Nonprofits only

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pLL3.7m-psMEK1tight

Plasmid

#89362

Purpose

A lenti-viral vector expressing photoswitchable MEK1 with minimum basal activity in mammalian cells

Depositor

Michael Lin

Article

Zhou et al Science. 2017 Feb 24;355(6327):836-842.

Insert

psMEK1tight (MAP2K1 Synthetic, Human)

Use

Lentiviral

Tags

Human influenza hemagglutinin (HA) tag and pdDron…

Expression

Mammalian

Promoter

CMV

Available Since

April 10, 2017

Availability

Academic Institutions and Nonprofits only

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pLKO-Cre stuffer v3

Plasmid

#158030

Purpose

lenti-viral construct with Cre recombinase and U6 driven sgRNA casette (20bp sgRNA can be cloned by digesting the stuffer 1.8kb with BsmBI). NO Cas9

Depositor

Daniel Schramek

Article

Loganathan et al Science. 2020 Mar 13;367(6483):1264-1269

Type

Empty backbone

Expression

Mammalian

Available Since

Sept. 29, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pLL3.7m-psMEK1

Plasmid

#89361

Purpose

A lenti-viral vector expressing photoswitchable MEK1 in mammalian cells

Depositor

Michael Lin

Article

Zhou et al Science. 2017 Feb 24;355(6327):836-842.

Insert

psMEK1 (MAP2K1 Synthetic, Human)

Use

Lentiviral

Tags

Human influenza hemagglutinin (HA) tag and pdDron…

Expression

Mammalian

Promoter

CMV

Available Since

April 10, 2017

Availability

Academic Institutions and Nonprofits only

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Cas9-sgRNA-A

Plasmid

#149369

Purpose

Expresses SpCas9 and sgRNA targeting the lenti-CDDR reporter

Depositor

Tarek Abbas

Article

Eki et al Nucleic Acids Res. 2020 Oct 17. pii: 592

Inserts

Cas9
sgRNA-A
Puromycin resistance

Use

CRISPR

Tags

3xFLAG, Nucleoplasmin NLS, and SV40 NLS

Expression

Mammalian

Promoter

CBh, PGK, and U6

Available Since

Nov. 4, 2020

Availability

Academic Institutions and Nonprofits only

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