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pSFFV_sfCherry2(11)_TagBFP

Plasmid

#82606

Purpose

Expresses sfCherry2(11) and TagBFP fusion in mammalian cells

Depositor

Bo Huang

Publication

Feng et al Nat Commun. 2017 Aug 29;8(1):370. doi: 1

Insert

sfCherry2(11)_TagBFP

Use

Lentiviral

Expression

Mammalian

Mutation

changed Gly12 to Ala in sfCherry11+

Promoter

SFFV

Available since

June 29, 2017

Availability

Academic Institutions and Nonprofits only

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pX330S-7

Plasmid

#58783

Purpose

Expresses Cas9 nuclease and gRNA

Depositor

Takashi Yamamoto

Publication

Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

Insert

humanized S. pyogenes Cas9 nuclease

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Promoter

CBh

Available since

Nov. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pX330S-7

Plasmid

#58783

Purpose

Expresses Cas9 nuclease and gRNA

Depositor

Takashi Yamamoto

Publication

Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

Insert

humanized S. pyogenes Cas9 nuclease

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Promoter

CBh

Available since

Nov. 7, 2014

Availability

Academic Institutions and Nonprofits only

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p3E-MCS

Plasmid

#75174

Purpose

Multiple cloning site for insertion of gene of interest for C-terminal fusions.

Depositor

Nicholas Cole

Publication

Don et al Zebrafish. 2016 Sep 15.

Insert

Multiple Cloning Site

Use

Three prime entry vector for multisite gateway th…

Promoter

n/a

Available since

Oct. 6, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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p3E-MCS

Plasmid

#75174

Purpose

Multiple cloning site for insertion of gene of interest for C-terminal fusions.

Depositor

Nicholas Cole

Publication

Don et al Zebrafish. 2016 Sep 15.

Insert

Multiple Cloning Site

Use

Three prime entry vector for multisite gateway th…

Promoter

n/a

Available since

Oct. 6, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pLSLGFP.R

Plasmid

#51501

Purpose

Single component LSL vector with GFP. Rep is expressed from the complementary sense LIR promoter. GFP is between LIR and SIR and is driven from a 2X35S promoter. Rep is within the replicon

Depositor

Daniel Voytas

Publication

Baltes et al Plant Cell. 2014 Jan;26(1):151-63. doi:

Insert

GFP

Use

Plant t-dna plasmid

Promoter

2x35S

Available since

May 2, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pLSLGFP.R

Plasmid

#51501

Purpose

Single component LSL vector with GFP. Rep is expressed from the complementary sense LIR promoter. GFP is between LIR and SIR and is driven from a 2X35S promoter. Rep is within the replicon

Depositor

Daniel Voytas

Publication

Baltes et al Plant Cell. 2014 Jan;26(1):151-63. doi:

Insert

GFP

Use

Plant t-dna plasmid

Promoter

2x35S

Available since

May 2, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pFA6a-link-yomKate2-SpHis5

Plasmid

#44852

Purpose

Yeast tagging vector to create gene fusion with yeast optimized mKate2 with SpHis5 selection

Depositor

Wendell Lim, Kurt Thorn

Publication

Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

Insert

yomKate2

Expression

Yeast

Available since

Oct. 16, 2013

Availability

Academic Institutions and Nonprofits only

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pFA6a-link-yomKate2-SpHis5

Plasmid

#44852

Purpose

Yeast tagging vector to create gene fusion with yeast optimized mKate2 with SpHis5 selection

Depositor

Wendell Lim, Kurt Thorn

Publication

Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

Insert

yomKate2

Expression

Yeast

Available since

Oct. 16, 2013

Availability

Academic Institutions and Nonprofits only

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pCDNA3 IRF9-STAT2C

Plasmid

#37544

Depositor

Curt Horvath

Publication

Kraus et al J Biol Chem. 2003 Apr 11;278(15):13033-8

Insert

IRF-9 STAT2 C (IRF9, STAT2 Human)

Expression

Mammalian

Promoter

CMV

Available since

March 17, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pCDNA3 IRF9-STAT2C

Plasmid

#37544

Depositor

Curt Horvath

Publication

Kraus et al J Biol Chem. 2003 Apr 11;278(15):13033-8

Insert

IRF-9 STAT2 C (IRF9, STAT2 Human)

Expression

Mammalian

Promoter

CMV

Available since

March 17, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pSLQ2812 pPB: CAG-GID1-VPR-IRES-Puro-WPRE PGK-GAI-tagBFP-SpdCas9

Plasmid

#84240

Purpose

Expresses GA-inducible VPR-Sp dCas9

Depositor

Stanley Qi

Publication

Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

Inserts

GAI-tagBFP-Sp dCas9
GID1-VPR

Use

CRISPR and Synthetic Biology; Piggybac

Tags

2xNLS (SV40), GAI, GID1, HA tag, IRES-Puro, and t…

Expression

Mammalian

Promoter

CAG and PGK

Available since

Nov. 9, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pSLQ2812 pPB: CAG-GID1-VPR-IRES-Puro-WPRE PGK-GAI-tagBFP-SpdCas9

Plasmid

#84240

Purpose

Expresses GA-inducible VPR-Sp dCas9

Depositor

Stanley Qi

Publication

Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

Inserts

GAI-tagBFP-Sp dCas9
GID1-VPR

Use

CRISPR and Synthetic Biology; Piggybac

Tags

2xNLS (SV40), GAI, GID1, HA tag, IRES-Puro, and t…

Expression

Mammalian

Promoter

CAG and PGK

Available since

Nov. 9, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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LeGO-G/BSD

Plasmid

#27354

Depositor

Boris Fehse

Publication

Weber et al Mol Ther. 2008 Apr . 16(4):698-706.

Insert

U6 promoter, SFFV promoter, eGFP/BSD-Fusion

Use

Lentiviral

Expression

Mammalian

Available since

April 24, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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LeGO-G/BSD

Plasmid

#27354

Depositor

Boris Fehse

Publication

Weber et al Mol Ther. 2008 Apr . 16(4):698-706.

Insert

U6 promoter, SFFV promoter, eGFP/BSD-Fusion

Use

Lentiviral

Expression

Mammalian

Available since

April 24, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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RCAS-myrAkt

Plasmid

#11547

Depositor

Sandra Orsulic

Publication

Orsulic et al Cancer Cell. 2002 Feb . 1(1):53-62.

Insert

Protein Kinase B (Akt1 Mouse)

Use

Retroviral

Tags

HA tag and myr signal

Available since

April 5, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles
RCAS-myrAkt

Plasmid

#11547

Depositor

Sandra Orsulic

Publication

Orsulic et al Cancer Cell. 2002 Feb . 1(1):53-62.

Insert

Protein Kinase B (Akt1 Mouse)

Use

Retroviral

Tags

HA tag and myr signal

Available since

April 5, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles
mRFP-FKBP12-5ptpase domain

Plasmid

#67516

Purpose

Recruitable human type IV 5-phosphatase domain (214-644)

Depositor

Tamas Balla

Publication

Varnai et al J Cell Biol. 2006 Nov 6;175(3):377-82.

Insert

FKBP12-5ptpase enzyme (INPP5E, FKBP1A Human)

Tags

mRFP

Mutation

INPP5E has C641A to destroy the C-terminal CAAX d…

Available since

Sept. 9, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles
mRFP-FKBP12-5ptpase domain

Plasmid

#67516

Purpose

Recruitable human type IV 5-phosphatase domain (214-644)

Depositor

Tamas Balla

Publication

Varnai et al J Cell Biol. 2006 Nov 6;175(3):377-82.

Insert

FKBP12-5ptpase enzyme (INPP5E, FKBP1A Human)

Tags

mRFP

Mutation

INPP5E has C641A to destroy the C-terminal CAAX d…

Available since

Sept. 9, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles
delta DE OCT4-GFP-2A-PURO transgenic reporter

Plasmid

#52381

Purpose

BAC recombineering generated construct, used as a reporter for OCT4 expression

Depositor

Jacob Hanna

Publication

Gafni et al Nature. 2013 Dec 12;504(7479):282-6. doi

Inserts

GFP-2A-puro
OCT4 (POU5F1 Human)

Expression

Mammalian

Mutation

deleted -3194 - -1768 upstream from OCT4 ATG

Promoter

OCT4

Available since

June 6, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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