We narrowed to 28,877 results for: pET

1. pET22-HT/plsA

   Plasmid

   #73625

   Purpose

   Produces Escherichia coli adenylate kinase (PlsA), N-terminal His6 tag

   Depositor

   James Williamson, T. J. Kappock

   Article

   Schultheisz et al ACS Chem Biol. 2008 Aug 15;3(8):499-511.

   Insert

   adenylate kinase (adk Escherichia coli str. K-12 substr. MG1655)
   

   Tags

   His6

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Feb. 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET31b-T7-Spinach2-Ct

   Plasmid

   #79158

   Purpose

   Expresses the Ct biosensor under a T7 promoter

   Depositor

   Ming Hammond

   Article

   Wang et al Nucleic Acids Res. 2016 Jul 5. pii: gkw5

   Insert

   T7-tRNA-Spinach2-Ct
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET31b-T7-Spinach2-Dp

   Plasmid

   #79159

   Purpose

   Expresses the Dp biosensor under a T7 promoter

   Depositor

   Ming Hammond

   Article

   Wang et al Nucleic Acids Res. 2016 Jul 5. pii: gkw5

   Insert

   T7-tRNA-Spinach2-Dp
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET28-MHL

   Plasmid

   #26096

   Purpose

   SGC Empty backbone for bacterial expression

   Depositor

   Cheryl Arrowsmith

   Type

   Empty backbone

   Tags

   6xHis and TEV cleavage site

   Expression

   Bacterial

   Promoter

   T7-lacO (lactose/IPTG inducible)

   Available Since

   Aug. 20, 2010

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pEThT-RvLEAM

   Plasmid

   #90033

   Purpose

   To express His-tagged RvLEAM in bacteria

   Depositor

   Takekazu Kunieda

   Article

   Tanaka et al PLoS One. 2015 Feb 12;10(2):e0118272. eC

   Insert

   RvLEAM
   

   Tags

   His

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   May 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET23a-mUbc9

   Plasmid

   #53137

   Purpose

   E.coli expression of full length untagged mouse Ubc9 (protein sequence identical to human)

   Depositor

   Frauke Melchior

   Article

   Pichler et al Cell. 2002 Jan 11;108(1):109-20.

   Insert

   Ubc9 (Ube2i Mouse)
   

   Tags

   untagged

   Expression

   Bacterial

   Mutation

   protein sequence is identical to human

   Promoter

   T7

   Available Since

   Sept. 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET30-2-GPD1

   Plasmid

   #83911

   Purpose

   stable overexpression

   Depositor

   David Sabatini

   Article

   Pacold et al Nat Chem Biol. 2016 Jun;12(6):452-8. doi

   Insert

   glycerol-3-phosphate dehydrogenase 1 (GPD1 Human)
   

   Tags

   6x His tags

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET_RTX-6xHis

   Plasmid

   #145029

   Purpose

   pET vector for IPTG-inducible, T7-based expression of RTX bearing a C-terminal 6xHis tag

   Depositor

   Andrew Ellington

   Article

   Bhadra et al BioProtocol 2021

   Insert

   RTX
   

   Tags

   6xHis

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   April 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET28a-SnoopCatcher

   Plasmid

   #72322

   Purpose

   Bacterial expression of SnoopCatcher, an engineered protein forming a spontaneous covalent bond with its cognate peptide partner SnoopTag.

   Depositor

   Mark Howarth

   Article

   Veggiani et al Proc Natl Acad Sci U S A. 2016 Jan 19. p

   Insert

   pET28a-SnoopCatcher
   

   Tags

   His6 tag

   Expression

   Bacterial

   Mutation

   changed G842T and D848G of S. pneumoniae adhesin …

   Promoter

   T7

   Available Since

   Feb. 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET15b-RvLEAMshort

    Plasmid

    #218122

    Purpose

    Expresses truncated RvLEAM protein under IPTG induction in bacterial host cells.

    Depositor

    Ci Ji Lim

    Article

    Abe et al bioRxiv [Preprint]. 2024 Feb 11:2024.02.

    Insert

    RvLEAMshort
    

    Tags

    6xHIS

    Expression

    Bacterial

    Mutation

    Truncated form (58-181aa)

    Promoter

    T7

    Available Since

    April 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET_RTX_(exo-)

    Plasmid

    #102786

    Purpose

    Expresses the Reverse Transcriptase gene (3'-5' exonuclease deficient mutant)

    Depositor

    Andrew Ellington

    Article

    Ellefson et al Science. 2016 Jun 24;352(6293):1590-3. d

    Insert

    reverse transcription xenopolymerase with 3'-5' deficient exonuclease
    

    Expression

    Bacterial

    Available Since

    July 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET24_NusA-Tus

    Plasmid

    #165959

    Purpose

    Expression of soluble E. coli Tus protein

    Depositor

    Keith Pardee

    Article

    Norouzi et al ACS Synth Biol. 2021 Jun 23. doi: 10.102

    Insert

    Tus
    

    Tags

    His-NusA-Tev

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET24b-APEX2

    Plasmid

    #111702

    Purpose

    For IPTG-inducible expression of APEX2 in E. coli

    Depositor

    Jessica Seeliger

    Article

    Ganapathy et al ACS Infect Dis. 2018 Apr 30. doi: 10.102

    Insert

    APEX2
    

    Tags

    6xHis and V5

    Expression

    Bacterial

    Promoter

    T7 promoter

    Available Since

    Sept. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET28 MBP_PrA/G-H6

    Plasmid

    #176077

    Purpose

    Express His6 tagged MBP_PrA/G in Escherichia coli

    Depositor

    Tom Rapoport

    Article

    Wu et al Proc Natl Acad Sci U S A. 2021 Oct 12;11

    Insert

    MBP PrA/G-H6
    

    Tags

    His tag

    Expression

    Bacterial

    Promoter

    T7 Promoter

    Available Since

    Oct. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET28a MDHC_YEAST

    Plasmid

    #204232

    Purpose

    Bacterial expression of yeast cytosolic malate dehydrogenase; Use in CURES (integration of research into undergraduate teaching labs).

    Depositor

    Malate dehydrogenase CUREs Community (MCC)

    Insert

    MDHC_YEAST (MDH2 Budding Yeast)
    

    Tags

    6X His

    Expression

    Bacterial

    Promoter

    Lac

    Available Since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. miniTurbo-His6_pET21a

    Plasmid

    #107178

    Purpose

    expresses His6-tagged miniTurbo in bacteria

    Depositor

    Alice Ting

    Article

    Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

    Insert

    miniTurbo (BirA mutant)
    

    Expression

    Bacterial

    Mutation

    aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …

    Available Since

    May 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET28-mE1

    Plasmid

    #32534

    Depositor

    Jorge Eduardo Azevedo

    Article

    Carvalho et al Mol Biotechnol. 2011 Oct 20.

    Insert

    E1, ubiquitin-activating enzyme (Uba1 Mouse)
    

    Tags

    His tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 18, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET22b RNase A

    Plasmid

    #58903

    Purpose

    Bacterial expression of bovine pancreatic ribonuclease A

    Depositor

    Ronald Raines

    Article

    delCardayre et al Protein Eng. 1995 Mar;8(3):261-73.

    Insert

    RNase A (RNASE1 Bovine)
    

    Tags

    pelB sequence

    Expression

    Bacterial

    Mutation

    See Depositor Comments below

    Promoter

    T7

    Available Since

    Feb. 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET28a MDHM_YEAST

    Plasmid

    #204231

    Purpose

    Bacterial expression of yeast mitochondrial malate dehydrogenase; Use in CURES (integration of research into undergraduate teaching labs).

    Depositor

    Malate dehydrogenase CUREs Community (MCC)

    Insert

    MDHM_YEAST (MDH1 Budding Yeast)
    

    Tags

    6X His

    Expression

    Bacterial

    Mutation

    Does not include transit peptide and includes a M…

    Promoter

    Lac

    Available Since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET12a-PAPI

    Plasmid

    #19961

    Depositor

    Jonathan Tait

    Article

    Wood et al Blood. 1996 Sep 1. 88(5):1873-80.

    Insert

    Annexin V (ANXA5 Human)
    

    Expression

    Bacterial

    Available Since

    Dec. 16, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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21. pET20b- hTPI

    Plasmid

    #50723

    Purpose

    overexpression of human TPI1 in E.coli

    Depositor

    Markus Ralser

    Article

    Gruning et al Open Biol. 2014 Mar 5;4(3):130232. doi:

    Insert

    Triosephosphate isomerase 1 (TPI1 Human)
    

    Tags

    6xHIS tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Jan. 30, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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22. pET28a(+)/FaGH16A

    Plasmid

    #100910

    Purpose

    Expression of the Beta-1,3-1,4-Endo-Laminarinase FaGH16A in E.coli

    Depositor

    Jan-Hendrik Hehemann

    Article

    Becker et al Appl Environ Microbiol. 2017 Feb 17. pii

    Insert

    FaGH16A
    

    Tags

    His-Tag

    Expression

    Bacterial

    Available Since

    Sept. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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23. pET28a(+)/FbGH30

    Plasmid

    #86463

    Purpose

    Expression of the Beta-1,6-Exo-Laminarinase FbGH30 in E.coli

    Depositor

    Jan-Hendrik Hehemann

    Article

    Becker et al Appl Environ Microbiol. 2017 Feb 17. pii

    Insert

    FbGH30
    

    Tags

    His-Tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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24. pET28a(+)/FbGH17A

    Plasmid

    #100911

    Purpose

    Expression of the Beta-1,3-Endo-Laminarinase FbGH17A in E.coli

    Depositor

    Jan-Hendrik Hehemann

    Insert

    FbGH17A
    

    Tags

    His-Tag

    Expression

    Bacterial

    Available Since

    March 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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25. pET47b-T7-enAsCas12f

    Plasmid

    #204642

    Purpose

    Expression of enAsCas12f in E. coli

    Depositor

    Weixin Tang

    Article

    Wu et al Nat Chem Biol. 2023 Jul 3. doi: 10.1038/

    Insert

    enAsCas12f
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    D196K, N199K, G276R, N328G, D364R

    Promoter

    T7

    Available Since

    Aug. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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26. pET28-SaCas9

    Plasmid

    #178901

    Purpose

    SaCas9 (Cas9 from Staphylococcus aureus)

    Depositor

    Hendrik Milting

    Article

    Flottmann et al STAR Protoc. 2022 Apr 5;3(2):101276. doi

    Insert

    SaCas9
    

    Tags

    His6-Tag, bipartite nuclear localization signal f…

    Expression

    Bacterial

    Available Since

    July 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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27. pET30a-HS-Tn5

    Plasmid

    #127916

    Purpose

    Prokaryotic expression of Hyperstable Tn5 (HS-Tn5) for Illumina library preperation. Developed for the large scale ChIP-Seq and ATAC-Seq experiments for the fruitENCODE and Maize Cistrome projects.

    Depositor

    Silin Zhong

    Article

    Tu et al Nat Commun. 2020 Oct 9;11(1):5089. doi:

    Insert

    Hyperstable Tn5
    

    Tags

    E coli elongation factor Ts and Mxe intein - Chit…

    Expression

    Bacterial

    Mutation

    E54K, L372P

    Promoter

    T7

    Available Since

    July 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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28. pET28b-PunyEEER

    Plasmid

    #208322

    Purpose

    Bacterial expression of the His-tagged peptide PunyEEER.

    Depositor

    Kimberly Beatty

    Article

    Suyama et al Biochemistry. 2023 Jun 6;62(11):1735-174

    Insert

    PunyERRE
    

    Tags

    DBCO-Tag, His-Tag

    Expression

    Bacterial

    Available Since

    April 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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29. pET28b-TinyERRE

    Plasmid

    #208320

    Purpose

    Bacterial expression of the His-tagged peptide TinyERRE.

    Depositor

    Kimberly Beatty

    Article

    Suyama et al Biochemistry. 2023 Jun 6;62(11):1735-174

    Insert

    TinyERRE
    

    Tags

    DBCO-Tag, His-Tag

    Expression

    Bacterial

    Available Since

    Feb. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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30. UbcH5c WT pET28a

    Plasmid

    #12643

    Depositor

    Rachel Klevit

    Article

    Brzovic et al Mol Cell. 2006 Mar 17. 21(6):873-80.

    Insert

    UbcH5c - Ubiquitin conjugating enzyme (UBE2D3 Human)
    

    Expression

    Bacterial

    Available Since

    July 31, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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