We narrowed to 5,958 results for: NAP

1. pLenti-hPGK-eGFP-SynaptoZip

   Plasmid

   #122523

   Purpose

   Lentiviral expression of the synaptic activity-marker SynaptoZip fused to eGFP driven by hPGK promoter

   Depositor

   Antonio Malgaroli

   Article

   Ferro et al Nat Commun. 2017 Oct 31;8(1):1229. doi:

   Insert

   eGFP-SynaptoZip (Vamp2 Rat)
   

   Use

   Lentiviral

   Tags

   Myc and eGFP

   Expression

   Mammalian

   Mutation

   Promoter

   hPGK

   Available since

   March 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pUC CBA-SpCas9D10Anickase.EF1a-BFP.sgLMNApair4

   Plasmid

   #98975

   Purpose

   Cas9 Homologous Recombination Reporter. SpCas9D10A nickase and a pair of sgRNAs targeting hLMNA. Generates breaks with 94bp 3' overhangs. TagBFP.

   Depositor

   Jan Karlseder

   Article

   Arnoult et al Nature. 2017 Sep 20;549(7673):548-552. d

   Insert

   LMNA sgRNAs pair 4 and Cas9(D10A)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET28a_DmKHC[1-421]-SNAP-6xHis

   Plasmid

   #196975

   Purpose

   Bacterial expression plasmid for His tag-based purification of Drosophila melanogaster kinesin heavy chain [1-421] with C-terminal SNAP tag and C-terminal 6xHis tag

   Depositor

   Lukas Kapitein

   Article

   Deguchi et al Science. 2023 Mar 10;379(6636):1010-1015

   Insert

   Kinesin Heavy Chain (Khc Fly)
   

   Use

   Tags

   6xHis and SNAP

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available since

   July 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. phage UbiC SNAP-KDEL

   Plasmid

   #104982

   Purpose

   Lentiviral vector expressing SNAP-KDEL for ER labeling

   Depositor

   Jeffrey Chao

   Article

   Voigt et al Cell Rep. 2017 Dec 26;21(13):3740-3753.

   Insert

   SNAP-KDEL
   

   Use

   Lentiviral

   Tags

   Secretion signal

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   UbiC

   Available since

   Feb. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pattB-synaptobrevin-GAL4-hsp70

   Plasmid

   #46107

   Depositor

   Christopher Potter

   Article

   Riabinina et al Nat Methods. 2015 Jan 12. doi: 10.1038/n

   Insert

   GAL4
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Aug. 12, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-hSyn-eGFP-SynaptoZip

   Plasmid

   #122525

   Purpose

   AAV expression of the synaptic activity-marker SynaptoZip fused to eGFP driven by human Synapsin promoter

   Depositor

   Antonio Malgaroli

   Article

   Ferro et al Nat Commun. 2017 Oct 31;8(1):1229. doi:

   Insert

   eGFP-SynaptoZip (Vamp2 Rat)
   

   Use

   AAV

   Tags

   Myc and eGFP

   Expression

   Mammalian

   Mutation

   Promoter

   hSyn

   Available since

   March 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pFCK(1.3)GFP-SynaptophysW

   Plasmid

   #27235

   Depositor

   Pavel Osten

   Article

   Dittgen et al Proc Natl Acad Sci U S A. 2004 Dec 28. 1

   Insert

   EGFP
   

   Use

   Lentiviral

   Tags

   Synaptophys-GFP

   Expression

   Mutation

   Promoter

   Available since

   March 11, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-hSynapsin1-axon-GCaMP6m

   Plasmid

   #111261

   Purpose

   For enriched expression of GCaMP6m in axons

   Depositor

   Lin Tian

   Article

   Broussard et al Nat Neurosci. 2018 Sep;21(9):1272-1280.

   Has Service

   AAV9

   Insert

   axon-GCaMP6m
   

   Use

   AAV

   Tags

   GAP43 palmitoylation domain

   Expression

   Mammalian

   Mutation

   Promoter

   hSynapsin1

   Available since

   June 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pFSW FLEx Synapsin-FLAG

   Plasmid

   #139653

   Purpose

   Conditional expression of Synapsin-FLAG

   Depositor

   Harold MacGillavry

   Article

   Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

   Insert

   Synapsin-FLAG
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Synapsin

   Available since

   June 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV hSynapsin flexed SV-tag

    Plasmid

    #163686

    Purpose

    express SV-Tag construct in a Cre-dependent manner

    Depositor

    Bernardo Sabatini

    Article

    Chantranupong et al Elife. 2020 Oct 12;9. pii: 59699. doi: 1

    Insert

    synaptophysin 9X HA
    

    Use

    AAV

    Tags

    9X HA

    Expression

    Mammalian

    Mutation

    Promoter

    synapsin

    Available since

    Jan. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pUC CBA-SpCas9D10Anickase.EF1a-BFP.sgLMNApair1

    Plasmid

    #98972

    Purpose

    Cas9 Homologous Recombination Reporter. SpCas9D10A nickase and a pair of sgRNA targeting hLMNA. Generates breaks with 44bp 5' overhangs. TagBFP.

    Depositor

    Jan Karlseder

    Article

    Arnoult et al Nature. 2017 Sep 20;549(7673):548-552. d

    Insert

    LMNA sgRNAs pair 1 and Cas9(D10A)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. His-ZZ-TEV-SNAPf-DYNC2H1

    Plasmid

    #132524

    Purpose

    Full length human dynein 2 heavy chain DYNC2H1, Sf9 codon optimised. With 8xHis-ZZ tag, TEV cleavage site, SNAPf tag, precission cleavage site at N-terminus. Assembled from synthesised fragments

    Depositor

    Andrew Carter

    Article

    Toropova et al Nat Struct Mol Biol. 2019 Aug 26. pii: 1

    Insert

    DYNC2H1 (DYNC2H1 Human)
    

    Use

    Tags

    8x His, Linker-TEV-linker-TEV, Precission cleavag…

    Expression

    Insect

    Mutation

    Codon optimised for Sf9 expression

    Promoter

    Available since

    Oct. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pFastBac(His10-SNAP-Csk)

    Plasmid

    #177895

    Purpose

    Baculo expression of His10 tag fused to SNAPtag and human Csk

    Depositor

    Ron Vale

    Article

    Hui et al Nat Struct Mol Biol. 2014 Feb;21(2):133-

    Insert

    Csk (CSK Human)
    

    Use

    Tags

    10xHis-TEV-TVMV-SNAP tag

    Expression

    Insect

    Mutation

    Promoter

    Polyhedrin

    Available since

    Oct. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBS-TRE-SNAPf-WPRE

    Plasmid

    #104106

    Purpose

    Expresses SNAPf under the TRE promoter in mammalian cells.

    Depositor

    Takeshi Imai

    Article

    Sakaguchi et al eLife. 2018 Nov 20;7. pii: 40350. doi: 1

    Insert

    SNAPf
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE

    Available since

    June 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pUC CBA-SpCas9D10Anickase.EF1a-BFP.sgLMNApair2

    Plasmid

    #98973

    Purpose

    Cas9 Homologous Recombination Reporter. SpCas9D10A nickase and a pair of sgRNAs targeting hLMNA. Generates breaks with 96bp 5' overhangs. TagBFP.

    Depositor

    Jan Karlseder

    Article

    Arnoult et al Nature. 2017 Sep 20;549(7673):548-552. d

    Insert

    LMNA sgRNAs pair 2 and Cas9(D10A)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pGEX6-2 SNAP-Syk tSH2

    Plasmid

    #113028

    Purpose

    for purification of Syk tSh2. Purified protein will have a snap tag that can be conjugated to fluorescent dyes.

    Depositor

    Ron Vale

    Article

    Morrissey et al Elife. 2018 Jun 4;7. pii: 36688. doi: 10

    Insert

    SNAP-Syk tSH2
    

    Use

    Tags

    SNAP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLenti-hPGK-mCherry-SynaptoZip

    Plasmid

    #177317

    Purpose

    Lentiviral expression of the synaptic activity-marker SynaptoZip fused to mCherry driven by hPGK promoter

    Depositor

    Antonio Malgaroli

    Article

    Lamanna et al Sci Rep. 2022 Jun 30;12(1):11055. doi: 1

    Insert

    mCherry-SynaptoZip (Vamp2 Rat)
    

    Use

    Lentiviral

    Tags

    Myc and mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAAV.hSynap-FLEX.SF-Venus-iGluSnFR.A184S

    Plasmid

    #106183

    Purpose

    Tight affinity yellow glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

    Depositor

    Loren Looger

    Article

    Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

    Has Service

    AAV1

    Insert

    SF-Venus-iGluSnFR.A184S
    

    Use

    AAV

    Tags

    Expression

    Mutation

    SFGFP: T203Y, F46L, T65G, S72A GltI: A184S

    Promoter

    hSynapsin

    Available since

    July 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pFastBac_His-ZZ-TEV-Lis1-SNAPf

    Plasmid

    #133073

    Purpose

    Plasmid for insect cell expression of human full-length LIS1 with an N terminal His-ZZ-LTLT tag and C terminal SNAPf tag

    Depositor

    Simon Bullock, Andrew Carter

    Article

    Baumbach et al Elife. 2017 Apr 13;6. pii: e21768. doi:

    Insert

    Lis1 (PAFAH1B1 Human)
    

    Use

    Tags

    8x His, Linker-TEV-linker_TEV, SNAPf, and ZZ

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Oct. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pattB-synaptobrevin-14-LEXA-QF-hsp70

    Plasmid

    #46123

    Depositor

    Christopher Potter

    Article

    Riabinina et al Nat Methods. 2015 Jan 12. doi: 10.1038/n

    Insert

    LexA-QF
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Aug. 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge