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Showing: 601 - 620 of 681 results

  5. pIMVAd40R

    Plasmid
    #98309
    Purpose
    Enhancing the lower-part mevalonate pathway; overexpressing yeast mevalonate pathway genes under the control of GAL promoters; integrated into ERG9 locus;
    Depositor
    Claudia Vickers
    Article
    Peng et al Metab Eng. 2018 Feb 19. pii: S1096-7176(
    Insert
    ERG9C-terminal-TURA3- PGAL7>MVD1>TPRM9-PGAL2>-ERG12>TNATTIDP1
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Feb. 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    98309-plasmid-map-sequence-id-182601

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  6. pIMVAd39T

    Plasmid
    #98303
    Purpose
    Enhancing the lower-part mevalonate pathway; overexpressing yeast mevalonate pathway genes under the control of consitutive promoters or GAL2 promoter; integrated into PDC5 locus; disrupting PDC5.
    Depositor
    Claudia Vickers
    Article
    Peng et al Biotechnol Biofuels. 2017 Feb 21;10:43.
    Insert
    pdc5(-277,-32)-PGAL2>ERG12>TNAT5-PTEF2>ERG8>TIDP1-TIDP1
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Aug. 7, 2017
    Availability
    Academic Institutions and Nonprofits only
    98303-plasmid-map-sequence-id-182779

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  7. mutpRL-TK 3xUTR

    Plasmid
    #40758
    Purpose
    To evaluate the efficacy of silencing and the extent of cooperativity directed by a small silencing RNA bound at one or multiple sites on an mRNA
    Depositor
    Phillip Zamore
    Article
    Broderick et al RNA. 2011 Oct;17(10):1858-69. Epub 2011
    Insert
    3 copies of the CXCR4 small RNA target site
    Use
    Luciferase
    Tags
    luciferase
    Expression
    Mutation
    mutated from TAG to CTC at nt 387–389 of the Reni…
    Promoter
    TK
    Available since
    Sept. 26, 2013
    Availability
    Academic Institutions and Nonprofits only

  10. pPuroR-MCS-GAL4-MiniWhite

    Plasmid
    #165890
    Purpose
    Puromycin resistant GAL4 driver vector with Mini-w+ CDS eye marker. Enhancer grammar GB20 entry point for custom enhancers. Cut-and-paste cloning can be used. Purple-white bacteria colony screening.
    Depositor
    Koen Venken
    Article
    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi
    Insert
    Selectable Empty Enhancer Driver
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    165890-plasmid-map-sequence-id-329406

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  12. pILGFPEasy9R

    Plasmid
    #218291
    Purpose
    Plasmid-free in-situ promoter cloning and characterisation system in S. cerevisiae, allows the characterisation of a bidirectional promoter using yEGFP and E2-Crimson as reporters.
    Depositor
    Claudia Vickers
    Article
    McDonnell et al bioRxiv 2024.03.26.586389
    Insert
    pURA3>KlURA3>tKlURA3-tPGK1KanMX>tAgTEF-pMAL32>MazF(RPL28i)-E2Crimson>tPDC1-pDDI2>ISceI>tSynth3>ura3(4,191)
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    May 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    218291-plasmid-map-sequence-id-433736

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  13. pToxAmp1

    Plasmid
    #218285
    Purpose
    Amplifying the gene copy number of heterologous genes (yEGFP and RelB) through ToxAmp (toxin-antitoxin-driven gene amplification) mechanism
    Depositor
    Claudia Vickers
    Article
    Evans et al bioRxiv 2024.01.30.578080
    Insert
    HO(-253, -1)-pRPL8B>RelB>tPDC1-pTEF1>yEGFP>tURA3-ARS712-HO(-731, -264)
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    May 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    218285-plasmid-map-sequence-id-433687

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  14. pG418R-MCS-LexA-VP16-MiniWhite

    Plasmid
    #165894
    Purpose
    G418 resistant LexA driver vector with Mini-w+ CDS eye marker. Enhancer grammar GB20 entry point for custom enhancers. Cut-and-paste cloning can be used. Purple-white bacteria colony screening.
    Depositor
    Koen Venken
    Article
    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi
    Insert
    Selectable Empty Enhancer Driver
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    165894-plasmid-map-sequence-id-338670

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  16. mutpRL-TK 6xUTR

    Plasmid
    #40761
    Purpose
    To evaluate the efficacy of silencing and the extent of cooperativity directed by a small silencing RNA bound at one or multiple sites on an mRNA
    Depositor
    Phillip Zamore
    Article
    Broderick et al RNA. 2011 Oct;17(10):1858-69. Epub 2011
    Insert
    6 copies of the CXCR4 small RNA target site
    Use
    Luciferase
    Tags
    luciferase
    Expression
    Mutation
    mutated from TAG to CTC at nt 387–389 of the Reni…
    Promoter
    TK
    Available since
    Sept. 26, 2013
    Availability
    Academic Institutions and Nonprofits only

  17. mutpRL-TK 2xUTR

    Plasmid
    #40757
    Purpose
    To evaluate the efficacy of silencing and the extent of cooperativity directed by a small silencing RNA bound at one or multiple sites on an mRNA
    Depositor
    Phillip Zamore
    Article
    Broderick et al RNA. 2011 Oct;17(10):1858-69. Epub 2011
    Insert
    2 copies of the CXCR4 small RNA target site
    Use
    Luciferase
    Tags
    luciferase
    Expression
    Mutation
    mutated from TAG to CTC at nt 387–389 of the Reni…
    Promoter
    TK
    Available since
    Sept. 26, 2013
    Availability
    Academic Institutions and Nonprofits only

  18. mutpRL-TK 4xUTR

    Plasmid
    #40759
    Purpose
    To evaluate the efficacy of silencing and the extent of cooperativity directed by a small silencing RNA bound at one or multiple sites on an mRNA
    Depositor
    Phillip Zamore
    Article
    Broderick et al RNA. 2011 Oct;17(10):1858-69. Epub 2011
    Insert
    4 copies of the CXCR4 small RNA target site
    Use
    Luciferase
    Tags
    luciferase
    Expression
    Mutation
    mutated from TAG to CTC at nt 387–389 of the Reni…
    Promoter
    TK
    Available since
    Sept. 26, 2013
    Availability
    Academic Institutions and Nonprofits only

  19. mutpRL-TK 5xUTR

    Plasmid
    #40760
    Purpose
    To evaluate the efficacy of silencing and the extent of cooperativity directed by a small silencing RNA bound at one or multiple sites on an mRNA
    Depositor
    Phillip Zamore
    Article
    Broderick et al RNA. 2011 Oct;17(10):1858-69. Epub 2011
    Insert
    5 copies of the CXCR4 small RNA target site
    Use
    Luciferase
    Tags
    luciferase
    Expression
    Mutation
    mutated from TAG to CTC at nt 387–389 of the Reni…
    Promoter
    TK
    Available since
    Sept. 26, 2013
    Availability
    Academic Institutions and Nonprofits only

  20. mutpRL-TK 1xUTR

    Plasmid
    #40756
    Purpose
    To evaluate the efficacy of silencing and the extent of cooperativity directed by a small silencing RNA bound at one or multiple sites on an mRNA
    Depositor
    Phillip Zamore
    Article
    Broderick et al RNA. 2011 Oct;17(10):1858-69. Epub 2011
    Insert
    CXCR4 small RNA target site
    Use
    Luciferase
    Tags
    luciferase
    Expression
    Mutation
    mutated from TAG to CTC at nt 387–389 of the Reni…
    Promoter
    TK
    Available since
    Sept. 26, 2013
    Availability
    Academic Institutions and Nonprofits only
Showing: 601 - 620 of 681 results