We narrowed to 740 results for: SG

1. pLAP-BUBR1-D882N

   Plasmid

   #114062

   Purpose

   transfection in mammalian cells, combination of fluorescent protein fusion and tandem affinity purification

   Depositor

   Geert Kops

   Article

   Suijkerbuijk et al Dev Cell. 2012 Jun 12;22(6):1321-9. doi:

   Insert

   BUBR1-D882N (BUB1B Human)
   

   Use

   Tags

   YFP-TEV-S- Human LAP

   Expression

   Mammalian

   Mutation

   C2823A and G2826A (siRNA-resistance), BUBR1-D882N

   Promoter

   Available since

   Nov. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLAP-BUBR1-K795R

   Plasmid

   #114060

   Purpose

   transfection in mammalian cells, combination of fluorescent protein fusion and tandem affinity purification

   Depositor

   Geert Kops

   Article

   Suijkerbuijk et al Dev Cell. 2012 Jun 12;22(6):1321-9. doi:

   Insert

   BUBR1-K795R (BUB1B Human)
   

   Use

   Tags

   YFP-TEV-S- Human LAP

   Expression

   Mammalian

   Mutation

   C2823A and G2826A (siRNA-resistance), BUBR1-K795R

   Promoter

   Available since

   Nov. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBrain-GFP-TACC3KDP∆682-688-shTACC3

   Plasmid

   #69116

   Purpose

   Dual-promoter plasmid to express knockdown-proof human TACC3 (682-688aa ch-TOG binding site deleted) tagged with EGFP along with shRNA to knock down endogenous TACC3 in mammalian cells.

   Depositor

   Stephen Royle

   Article

   Gutierrez-Caballero et al Biol Open. 2015 Jan 16;4(2):170-9. doi:

   Inserts

   Transforming Acidic Coiled Coil protein 3 (TACC3 Human)
   shTACC3
   

   Use

   RNAi

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Deletion of amino acids 682-688 (region containin…

   Promoter

   CMV

   Available since

   Oct. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRCIscript-crhr1_TAL1B

   Plasmid

   #44235

   Depositor

   Stephen Ekker, Karl Clark

   Article

   Bedell et al Nature. 2012 Nov 1;491(7422):114-8. doi:

   Insert

   crhr1 pTAL scaffold 1B (crhr1 Zebrafish)
   

   Use

   Mrna transcription vector

   Tags

   Expression

   Mutation

   Promoter

   T3

   Available since

   April 10, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRCIscript-crhr1_TAL1A

   Plasmid

   #44234

   Depositor

   Stephen Ekker, Karl Clark

   Article

   Bedell et al Nature. 2012 Nov 1;491(7422):114-8. doi:

   Insert

   crhr1 pTAL scaffold 1A (crhr1 Zebrafish)
   

   Use

   Mrna transcription vector

   Tags

   Expression

   Mutation

   Promoter

   T3

   Available since

   April 10, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. pScalps-Puro-FLAG_HA-Regnase-1 D141N

   Plasmid

   #222663

   Purpose

   Lentiviral vector to express mouse Regnase-1 D141N mutant tagged with FLAG-HA at N-term

   Depositor

   Silvia Monticelli

   Article

   Bataclan et al Life Sci Alliance. 2024 Jun 3;7(8):e2024

   Insert

   Zc3h12a (Zc3h12a Mouse)
   

   Use

   Lentiviral

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   mutated aspartic acid 141 to asparagine to abroga…

   Promoter

   SFFV

   Available since

   Jan. 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGL4.23-WIPI1promotor

   Plasmid

   #206840

   Purpose

   The plasmid contains the human WIPI1 promotor sequence (-1226 bp upstream to +329 bp downstream of the transcription start site) cloned into pGL4.23[luc2/minP] (NheI/HindIII).

   Depositor

   Tassula Proikas-Cezanne

   Article

   Sporbeck et al Commun Biol. 2023 Aug 24;6(1):872. doi:

   Insert

   WIPI1 promotor (partial)
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 21, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pUC57 mini-ZsGreen-P2A-FLAG-HA-Regnase-1 D141N

   Plasmid

   #222650

   Purpose

   For in vitro transcription of mouse Zc3h12a D141N mutant tagged with FLAG-HA at N-term

   Depositor

   Silvia Monticelli

   Article

   Bataclan et al Life Sci Alliance. 2024 Jun 3;7(8):e2024

   Insert

   Zc3h12a (Zc3h12a Mouse)
   

   Use

   In vitro transcription

   Tags

   FLAG and HA

   Expression

   Mutation

   mutated aspartic acid 141 to asparagine to abroga…

   Promoter

   T7

   Available since

   Oct. 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGECPL.BC10.MG

   Plasmid

   #223822

   Purpose

   Contains Cas9-editable barcode, marking guide (MG) for lineage tracing, Cre for Cas9 activation and loxP-mediated gene deletion, and Firefly Luciferase (FLuc) for live luminescence-based monitoring.

   Depositor

   Dawid Nowak

   Article

   Serio et al Cancer Discov. 2024 Jul 5. doi: 10.1158/

   Insert

   Ef1a-Driven Cre-P2A-FLuc
   

   Use

   CRISPR, Cre/Lox, Lentiviral, Luciferase, and Mous…

   Tags

   P2A

   Expression

   Mammalian

   Mutation

   Promoter

   Ef1a

   Available since

   Aug. 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMXs-IRES-blast 3xFlag-SPNS1(R76A)

    Plasmid

    #198570

    Purpose

    SPNS1(R76A) codon-optimized orf with n-terminal 3xFlag in pMXs-IRES-Blast vector

    Depositor

    Monther Abu-Remaileh

    Article

    Scharenberg et al Sci Adv. 2023 Apr 21;9(16):eadf8966. doi

    Insert

    SPNS1(R76A) with n-terminal 3xFlag (SPNS1 Human)
    

    Use

    Retroviral

    Tags

    3xFlag

    Expression

    Mammalian

    Mutation

    changed arginine R76 to alanine (R76A)

    Promoter

    Available since

    July 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMXs-IRES-blast GFP-SPNS1(E164K)

    Plasmid

    #198572

    Purpose

    SPNS1(E164K) codon-optimized orf with n-terminal 3xFlag GFP in pMXs-IRES-Blast vector

    Depositor

    Monther Abu-Remaileh

    Article

    Scharenberg et al Sci Adv. 2023 Apr 21;9(16):eadf8966. doi

    Insert

    SPNS1(E164K) with n-terminal 3xFlag-GFP fusion (SPNS1 Human)
    

    Use

    Retroviral

    Tags

    3xFlag-GFP

    Expression

    Mammalian

    Mutation

    changed glutamic acid E164 to lysine (E164K)

    Promoter

    Available since

    July 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCINeo-gp78 Mutant G2BR

    Plasmid

    #185355

    Purpose

    Assessing cellular requirement for G2BRgp78

    Depositor

    Allan Weissman

    Article

    Smith et al PLoS Biol. 2021 Dec 8;19(12):e3001474. d

    Insert

    gp78 (AMFR Human)
    

    Use

    Tags

    GST

    Expression

    Mammalian

    Mutation

    aa 595-600 KRFLNK to AGAAGG

    Promoter

    Available since

    Dec. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCDNA3-AUP1 A397D

    Plasmid

    #185342

    Purpose

    Assessing G2BR function in AUP1

    Depositor

    Allan Weissman

    Article

    Smith et al PLoS Biol. 2021 Dec 8;19(12):e3001474. d

    Insert

    AUP1 (AUP1 Human)
    

    Use

    Tags

    Flag, 6xHis

    Expression

    Mammalian

    Mutation

    A397D

    Promoter

    Available since

    May 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLKO.1-Teton-puro-shFDPS #2

    Plasmid

    #198760

    Purpose

    conditional knockdown of FDPS

    Depositor

    Hyonchol Jang

    Article

    Kim et al Exp Mol Med. 2018 Oct 17;50(10):137. doi

    Insert

    shFDPS #2 (FDPS Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLKO.1-Teton-puro-shFDPS #1

    Plasmid

    #198759

    Purpose

    conditional knockdown of FDPS

    Depositor

    Hyonchol Jang

    Article

    Kim et al Exp Mol Med. 2018 Oct 17;50(10):137. doi

    Insert

    shFDPS #1 (FDPS Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3.1(+)-Cue1pTM-MYC-UBE2G2-FLAG

    Plasmid

    #185347

    Purpose

    Membrane anchoring of UBE2G2 and co-IP. Encodes transmembrane domain of S. Cerevisiae Cue1p fused to MYC-UBE2G2 with C-terminal FLAG tag

    Depositor

    Allan Weissman

    Article

    Smith et al PLoS Biol. 2021 Dec 8;19(12):e3001474. d

    Insert

    Cue1p/Ube2G2 fusion (internal MYC tag)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLenti-mitoX4-mVenus

    Plasmid

    #185808

    Purpose

    Mitochondrial targetted (COX8) mVenus

    Depositor

    Dipayan Chaudhuri

    Article

    Balderas et al Nat Commun. 2022 May 19;13(1):2769. doi:

    Insert

    mito-mVenus
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLenti-mitoX4-FKBP-HA

    Plasmid

    #185658

    Purpose

    Mitochondrial targetted (Cox8) FKBP protein with C-terminal HA epitope

    Depositor

    Dipayan Chaudhuri

    Article

    Balderas et al Nat Commun. 2022 May 19;13(1):2769. doi:

    Insert

    mito-FKBP
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV_Donor/Reporter-COL4A3

    Plasmid

    #130279

    Purpose

    COL4A3 mutation-specific sgRNA under the control of U6 promoter; mCherry-sgRNA target-(out of frame)EGFP expression cassette; COL4A3 donor template

    Depositor

    Silvestro Conticello

    Article

    Daga et al Eur J Hum Genet. 2019 Nov 21. pii: 10.10

    Insert

    mCherry-eGFP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Dec. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV_COL4A3_self-cleavingCas9

    Plasmid

    #130281

    Purpose

    Expresses an spCas9 under the control of a CMV promoter; The spCas9 is flanked by COL4A3 sgRNA targets to induce its self-cleaving and inactivation

    Depositor

    Silvestro Conticello

    Article

    Daga et al Eur J Hum Genet. 2019 Nov 21. pii: 10.10

    Insert

    spCas9
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Dec. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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