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pCL1046

Plasmid

#252436

Purpose

Mitochondria-tethered construct for the RAPID-Release approach

Depositor

Andrew Bowman

Article

Apta-Smith et al EMBO J. 2018 Oct 1;37(19):e98714. doi: 1

Insert

Histone H3.2

Tags

mCherry-TVMVx2-FRB-OMP25

Expression

Mammalian

Promoter

CMV

Available Since

March 10, 2026

Availability

Academic Institutions and Nonprofits only

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AncPass

Plasmid

#196461

Purpose

Expresses Na,K-ATPase (ATP1A1 and ATP1B1 in pFastBac Dual vector) in insect cells

Depositor

Susanne Dobler

Article

Mohammadi et al bioRxiv 2025.07.04.662692

Inserts

ATP1A1
ATP1B1

Expression

Insect

Promoter

PH and p10

Available Since

Feb. 19, 2026

Availability

Academic Institutions and Nonprofits only

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AncPass+Q111E

Plasmid

#196462

Purpose

Expresses Na,K-ATPase (ATP1A1 and ATP1B1 in pFastBac Dual vector) in insect cells

Depositor

Susanne Dobler

Article

Mohammadi et al bioRxiv 2025.07.04.662692

Inserts

ATP1A1
ATP1B1

Expression

Insect

Promoter

PH and p10

Available Since

Feb. 19, 2026

Availability

Academic Institutions and Nonprofits only

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AncPass+6sub

Plasmid

#196463

Purpose

Expresses Na,K-ATPase (ATP1A1 and ATP1B1 in pFastBac Dual vector) in insect cells

Depositor

Susanne Dobler

Article

Mohammadi et al bioRxiv 2025.07.04.662692

Inserts

ATP1A1
ATP1B1

Expression

Insect

Promoter

PH and p10

Available Since

Feb. 19, 2026

Availability

Academic Institutions and Nonprofits only

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AncPass+7sub

Plasmid

#196464

Purpose

Expresses Na,K-ATPase (ATP1A1 and ATP1B1 in pFastBac Dual vector) in insect cells

Depositor

Susanne Dobler

Article

Mohammadi et al bioRxiv 2025.07.04.662692

Inserts

ATP1A1
ATP1B1

Expression

Insect

Promoter

PH and p10

Available Since

Feb. 19, 2026

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_Venus

Plasmid

#247761

Purpose

mammalian expression of Venus

Depositor

Alan Ashworth

Article

Abrams et al Cell Chem Biol. 2025 Nov 10:S2451-9456(2

Insert

Venus

Expression

Mammalian

Available Since

Nov. 11, 2025

Availability

Academic Institutions and Nonprofits only

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xenCGN(1-378)

Plasmid

#236504

Purpose

For expression of the head domain of Xenopus CGN in bacteria

Depositor

Sandra Citi

Article

D'Atri et al J Biol Chem. 2002 Aug 2;277(31):27757-64

Insert

CGN (cgn Frog)

Tags

His and Myc

Expression

Bacterial

Mutation

WT

Available Since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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pMB28

Plasmid

#223011

Purpose

Cas12a and Csy4 with 5' and 3' targets (I) for Nicotiana leaf assay

Depositor

Martin Parniske

Article

Bircheneder et al Plant J. 2024 Sep;119(5):2564-2577. doi:

Insert

Cas12a and Csy4 with 5' and 3' targets (I)

Use

CRISPR

Expression

Plant

Available Since

March 18, 2025

Availability

Academic Institutions and Nonprofits only

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pLY071c

Plasmid

#227940

Purpose

SICLOPPS precursor for cyclo-CHVVGVI

Depositor

Tina Wang

Article

Yang et al Nat Chem Biol. 2025 Jan 13. doi: 10.1038

Insert

Evolved Ssp SICLOPPS-CHVVGVI

Expression

Bacterial

Mutation

Evolved Ssp split inteins with multiple mutations…

Available Since

Nov. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pLY071e

Plasmid

#227941

Purpose

SICLOPPS precursor for cyclo-CDLGVFR

Depositor

Tina Wang

Article

Yang et al Nat Chem Biol. 2025 Jan 13. doi: 10.1038

Insert

Evolved Ssp SICLOPPS-CDLGVFR

Expression

Bacterial

Mutation

Evolved Ssp split inteins with multiple mutations…

Available Since

Nov. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pLY090a

Plasmid

#227938

Purpose

SICLOPPS precursor for cyclo-CRVWCAR

Depositor

Tina Wang

Article

Yang et al Nat Chem Biol. 2025 Jan 13. doi: 10.1038

Insert

Evolved Ssp SICLOPPS-CRVWCAR

Expression

Bacterial

Mutation

Evolved Ssp split inteins with multiple mutations…

Available Since

Nov. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pLY090b

Plasmid

#227939

Purpose

SICLOPPS precursor for cyclo-CRVYQVL

Depositor

Tina Wang

Article

Yang et al Nat Chem Biol. 2025 Jan 13. doi: 10.1038

Insert

Evolved Ssp SICLOPPS-CRVYQVL

Expression

Bacterial

Mutation

Evolved Ssp split inteins with multiple mutations…

Available Since

Nov. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pKJ247

Plasmid

#228714

Purpose

Mammalian expression of U6 epPsaCas12f RNF2 targeting guide

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Jiang et al Science 0, eadr6006

Insert

u6-RNF2 targeting guide

Use

CRISPR

Expression

Mammalian

Available Since

Nov. 25, 2024

Availability

Academic Institutions and Nonprofits only

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p2T_CAG_SpCas9PE2_5plinker_BlastR

Plasmid

#173066

Purpose

Expresses PE2

Depositor

Richard Sherwood

Article

Velimirovic et al Nat Commun. 2022 Jun 18;13(1):3512. doi:

Insert

5plinker

Expression

Mammalian

Available Since

Oct. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pSuperior.retro.neo GFP shScramble

Plasmid

#220357

Purpose

Retroviral expression vector for an shScramble

Depositor

David Kashatus

Article

Sweatt et al Mol Syst Biol. 2024 Sep 27. doi: 10.1038

Insert

shScramble

Use

RNAi and Retroviral

Promoter

H1

Available Since

Oct. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pET28-Butelase

Plasmid

#221848

Purpose

Expresses asparaginyl endopeptidase butelase

Depositor

Artur Krężel

Article

Singh et al Microb Cell Fact. 2024 Nov 30;23(1):325.

Insert

Butelase 1 ligase

Expression

Bacterial

Mutation

No

Available Since

Aug. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pNoRBS

Plasmid

#218167

Purpose

pET31b plasmid with short insert lacking ribosome binding site to increase 2',3'-cNMP levels

Depositor

Emily Weinert

Article

Chauhan et al ACS Cent Sci. 2022 Nov 23;8(11):1518-152

Insert

Short insert

Expression

Bacterial

Promoter

T7

Available Since

June 3, 2024

Availability

Academic Institutions and Nonprofits only

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pAN099

Plasmid

#220044

Purpose

Mammalian expression of type III-A crRNA from Streptococcus thermophilus

Depositor

Blake Wiedenheft

Article

Nemudraia et al Science. 2024 Apr 25:eadk5518. doi: 10.1

Insert

crRNA

Expression

Mammalian

Available Since

May 23, 2024

Availability

Academic Institutions and Nonprofits only

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KNtp-SUR1 core fusion

Plasmid

#216212

Purpose

for expression of KNtp-SUR1 fusion protein in BacMam system

Depositor

Lei Chen

Article

Wang et al Front Pharmacol. 2022 Jun 30;13:929684.

Insert

SUR1 core

Tags

GFP-MBP

Expression

Mammalian

Mutation

deletion of TMD0

Available Since

May 7, 2024

Availability

Academic Institutions and Nonprofits only

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pGL4_EGR1p-BC1490-luc2

Plasmid

#214654

Purpose

barcoded assay, MAPK sensor

Depositor

Michael Wehr

Article

Popovic et al STAR Protoc. 2024 Apr 16;5(2):102987. do

Insert

EGR1 promoter with barcode 1490

Use

Luciferase

Expression

Mammalian

Available Since

April 1, 2024

Availability

Academic Institutions and Nonprofits only

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