We narrowed to 8,454 results for: gnal

1. CUP1-∆ssCPY*-mNeonGreen in pRS315

   Plasmid

   #221160

   Purpose

   Fluorescent reporter for cytoplasmic aggregates under LEU2 selection marker

   Depositor

   Mark Leake

   Insert

   Carboxypeptidase Y (PRC1 Budding Yeast)
   

   Tags

   mNeonGreen

   Expression

   Yeast

   Mutation

   Deletion signal peptide, G255A mutation

   Available Since

   Nov. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pNIC-OmpA-NHSTIIT-GG

   Plasmid

   #215790

   Purpose

   Cloning of target gene with N terminal OmpA SEC pathway signal peptide His6 Twin Strep tag and TEV cleavage

   Depositor

   ASAP Discovery, Frank von Delft

   Article

   Fairhead et al bioRxiv 2024.02.13.579886

   Type

   Empty backbone

   Expression

   Bacterial

   Available Since

   April 25, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pNIC-SufI-NHSTIIT-GG

   Plasmid

   #215791

   Purpose

   Cloning of target gene with N terminal SufI TatA pathway signal peptide His6 Twin Strep tag and TEV cleavage

   Depositor

   ASAP Discovery, Frank von Delft

   Article

   Fairhead et al bioRxiv 2024.02.13.579886

   Type

   Empty backbone

   Expression

   Bacterial

   Available Since

   April 25, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. Cas9-T2A-H2B mCitirine

   Plasmid

   #210471

   Purpose

   Coexpression of Cas9 and H2B mCitrine

   Depositor

   Amro Hamdoun

   Insert

   Cas9-T2A-H2B mCitirine
   

   Use

   CRISPR and Synthetic Biology

   Tags

   mCitrine on C terminal of H2B

   Expression

   Mammalian

   Mutation

   Cas9 contains 3xFLAG tag and nuclear localization…

   Available Since

   Nov. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCW57.1-RFP_mmu-miR-292b-3p_sponge

   Plasmid

   #132623

   Purpose

   Expresses a TurboRFP transcript with test microRNA sponge for mmu-miR-292b-3p as 3'UTR; serves as template for other microRNA sponges

   Depositor

   Constance Ciaudo

   Insert

   stop codon, mmu-miR-292b-3p sponge sequence and polyA signal
   

   Use

   Lentiviral; Doxycycline inducible

   Expression

   Mammalian

   Promoter

   TRE

   Available Since

   Aug. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. MDKA-c009

   Plasmid

   #175463

   Purpose

   Protein expression/secretion in mammalian cells. Entire CDS with ectopic signal peptide, V21-D143

   Depositor

   Opher Gileadi

   Insert

   MDK (MDK Human)
   

   Tags

   His6 and PTPRS (Gg) SP

   Expression

   Mammalian

   Available Since

   Jan. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. YIplac211-KAR2-mEGFP-HDEL

   Plasmid

   #160467

   Purpose

   Yeast integration vector for the gene replacement of S. cerevisiae KAR2 fused to mEGFP with an ER retrieval signal

   Depositor

   Benjamin Glick

   Article

   Valbuena et al Traffic. 2020 May 15. doi: 10.1111/tra.1

   Insert

   KAR2-mEGFP-HDEL
   

   Expression

   Yeast

   Available Since

   March 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJT104

   Plasmid

   #25088

   Depositor

   Christopher Voigt

   Article

   Tabor et al Cell. 2009 Jun 26. 137(7):1272-81.

   Insert

   Edge detector delta luxI
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   Feb. 10, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTD103luxI_sfGFP

   Plasmid

   #48885

   Purpose

   Positive feedback plasmid from oscillator

   Depositor

   Jeff Hasty

   Article

   Prindle et al Nature. 2011 Dec 18;481(7379):39-44. doi

   Insert

   LuxI, sfGFP, and LuxR
   

   Use

   Synthetic Biology

   Tags

   ssrA tag (AANDENYALAA)

   Expression

   Bacterial

   Promoter

   pLux

   Available Since

   Feb. 6, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pQC membrane TdTomato IX

    Plasmid

    #37351

    Depositor

    Connie Cepko

    Article

    Beier et al Dev Biol. 2011 May 15;353(2):309-20. Epu

    Insert

    membrane TdTomato
    

    Tags

    Palmitoylation signal*

    Available Since

    July 30, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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11. pUAS-NanoLuc

    Plasmid

    #87696

    Purpose

    Gal4VP16 driven expression of NanoLuc

    Depositor

    Robert Campbell

    Article

    Zhang et al Nat Methods. 2017 Mar 13. doi: 10.1038/n

    Insert

    NanoLuc
    

    Tags

    nuclear localization signal

    Expression

    Mammalian

    Available Since

    April 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. myr-palm-mCherry2

    Plasmid

    #197910

    Purpose

    Monomer expression vector with plasma membrane localization signal

    Depositor

    Salvatore Chiantia

    Article

    Petrich et al Biophys J. 2021 Dec 21;120(24):5478-5490

    Insert

    myristoylated-palmitoylated-mCherry2
    

    Expression

    Mammalian

    Mutation

    none

    Available Since

    July 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCS2+ EGFP-3NLS

    Plasmid

    #165400

    Purpose

    Expresses nuclear localized EGFP

    Depositor

    Philipp Niethammer

    Article

    Enyedi et al Cell. 2016 May 19;165(5):1160-1170. doi:

    Insert

    Nuclear Localization Signal
    

    Use

    Avian, xenopus, zebrafish

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    SP6

    Available Since

    March 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDJSVT87

    Plasmid

    #109302

    Purpose

    Express periplasmic, outer membrane, or secreted proteins in E. coli - OmpA signal and His6 tag

    Depositor

    Daniel Slade

    Article

    Casasanta et al Methods Mol Biol. 2019;1960:123-138. doi

    Type

    Empty backbone

    Tags

    OmpA 1-27, 6His

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. nls-NgAgo-GK

    Plasmid

    #78253

    Purpose

    Mammalian expression of NgAgo (Argonaute from N. gregoryi).

    Depositor

    Chunyu Han

    Article

    Gao et al Nat Biotechnol. 2016 May 2. doi: 10.1038

    Insert

    NgAgo
    

    Tags

    a nuclear localization signal (NLS)

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTD103aiiA(Cm)

    Plasmid

    #48886

    Purpose

    Negative feedback plasmid from oscillator

    Depositor

    Jeff Hasty

    Article

    Prindle et al Nature. 2011 Dec 18;481(7379):39-44. doi

    Insert

    aiiA and LuxR
    

    Use

    Synthetic Biology

    Tags

    ssrA tag (AANDENYALAA)

    Expression

    Bacterial

    Promoter

    pLux

    Available Since

    Feb. 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. Ub-R-YFP

    Plasmid

    #11948

    Depositor

    Nico Dantuma

    Article

    Menendez-Benito et al Hum Mol Genet. 2005 Oct 1. 14(19):2787-9

    Insert

    Ub-R-YFP
    

    Expression

    Mammalian

    Mutation

    Ubiquitin fused to N-terminus of YFP. Arginine at…

    Available Since

    July 5, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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18. GPI-mNeonGreen

    Plasmid

    #197912

    Purpose

    Monomer expression vector with plasma membrane localization signal

    Depositor

    Salvatore Chiantia

    Article

    Petrich et al PLoS One. 2023 Aug 3;18(8):e0285486. doi

    Insert

    glycosylphosphatidylinositol-mNeonGreen
    

    Expression

    Mammalian

    Mutation

    none

    Available Since

    April 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pZE-PalkB-GFP

    Plasmid

    #44381

    Depositor

    Hal Alper

    Article

    Reed et al J Biotechnol. 2012 Apr 15;158(3):75-9. d

    Inserts

    GFP
    PalkB
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    PalkB

    Available Since

    April 17, 2013

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. pACYC-PLlambda-alkSm4-3

    Plasmid

    #44383

    Depositor

    Hal Alper

    Article

    Reed et al J Biotechnol. 2012 Apr 15;158(3):75-9. d

    Insert

    AlkSm4-3
    

    Use

    Synthetic Biology

    Mutation

    Q410I, S470T, K546E, E775D

    Promoter

    PLlambda

    Available Since

    July 15, 2013

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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