We narrowed to 42,187 results for: ids

1. pAAV-anti-GFP(LaG17) PAGER(TF)

   Plasmid

   #229997

   Purpose

   Expresses anti-GFP PAGER(TF) (high reversibility) in mammalian cells; used with NanoLuc-Arrestin-TEVp (Addgene #125228) and UAS-Firefly Luciferase reporter (Addgene #104840)

   Depositor

   Alice Ting

   Article

   Kalogriopoulos et al Nature. 2024 Dec 4. doi: 10.1038/s41586-

   Insert

   IL2SP-Aro6-LaG17-TEVcs-ALFA-KORD(RAA)-LOV-TEVcs-Gal4
   

   Use

   AAV

   Expression

   Mammalian

   Mutation

   V360A/R361A on KORD

   Promoter

   CMV

   Available Since

   Jan. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pIRES-hrGFP II-TET1_CD

   Plasmid

   #83570

   Purpose

   Expresses the catalytic domain of TET1 in mammalian cells

   Depositor

   Jean-Pierre Issa

   Article

   Jin et al Nucleic Acids Res. 2014 Jun;42(11):6956-

   Insert

   Tet Methylcytosine Dioxygenase 1 (TET1 Human)
   

   Tags

   3X FLAG tag and hr GFP II

   Expression

   Mammalian

   Mutation

   TET1 amino acids 1-1417 deleted

   Promoter

   CMV

   Available Since

   Aug. 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. ESR1-luciferase

   Plasmid

   #113351

   Purpose

   evaluate FOXA1 DIV motif enhancer activity near 50 kb of ESR1 gene

   Depositor

   Ralf Jauch

   Article

   Wang et al Nucleic Acids Res. 2018 Jun 20;46(11):54

   Insert

   ESR1-enhancer (ESR1 Human)
   

   Use

   Luciferase

   Available Since

   Sept. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-CaMKIIa-C1V1 (t/t)-TS-mCherry

   Plasmid

   #35500

   Purpose

   AAV expression of CaMKII-driven chimeric opsin variant C1V1 (E122T/E162T) for fast and potent optical excitation at red-shifted wavelengths.

   Depositor

   Karl Deisseroth

   Article

   Yizhar et al Nature. 2011 Jul 27;477(7363):171-8. doi

   Insert

   ChR1-VChR1 Chimera
   

   Use

   AAV

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   E122T and E162T

   Promoter

   CaMKIIa

   Available Since

   April 18, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEvol-pAcFRS.1.t1

   Plasmid

   #73545

   Purpose

   tRNA synthetase/tRNA pair for the in vivo incorporation of several non-standard amino acids, into proteins in E coli in response to the amber codon, TAG

   Depositor

   Farren Isaacs

   Article

   Amiram et al Nat Biotechnol. 2015 Dec;33(12):1272-127

   Inserts

   pAcFRS.1.t1
   pAcFRS.1.t1
   

   Expression

   Bacterial

   Available Since

   March 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-CaMKIIa-hChR2(C128S/D156A)-EYFP

   Plasmid

   #35501

   Purpose

   AAV expression of CaMKII-driven stabilized step function opsin (SSFO) for optogenetic activation.

   Depositor

   Karl Deisseroth

   Article

   Yizhar et al Nature. 2011 Jul 27;477(7363):171-8. doi

   Insert

   hChR2
   

   Use

   AAV

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   C128S and D156A

   Promoter

   CaMKIIa

   Available Since

   April 18, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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7. AAV N-SpyCas9-Intein, U6-gRNA scaffold (F+E)

   Plasmid

   #120293

   Purpose

   AAV Vector for expression of N-terminal SpyCas9 fragment with Intein and a U6-driven F+E gRNA scaffold

   Depositor

   Dominik Niopek

   Article

   Hoffmann et al Nucleic Acids Res. 2019 Apr 15. pii: 545

   Insert

   N-terminal fragment of SpyCas9
   

   Use

   AAV and CRISPR

   Tags

   SV40-NLS and split-intein

   Expression

   Mammalian

   Available Since

   Jan. 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-anti-TNFalpha(Ozoralizumab) PAGER(TF)

   Plasmid

   #230001

   Purpose

   Expresses anti-TNFalpha PAGER(TF) in mammalian cells; used with NanoLuc-Arrestin-TEVp (Addgene #125228) and UAS-Firefly Luciferase reporter (Addgene #104840)

   Depositor

   Alice Ting

   Article

   Kalogriopoulos et al Nature. 2024 Dec 4. doi: 10.1038/s41586-

   Insert

   IL2SP-Aro6-Ozoralizumab-TEVcs-ALFA-KORD(RAA)-LOV-TEVcs-Gal4
   

   Use

   AAV

   Expression

   Mammalian

   Mutation

   V360A/R361A on KORD

   Promoter

   CMV

   Available Since

   Jan. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAAV-anti-CCL2(8E10) PAGER(TF)

   Plasmid

   #230002

   Purpose

   Expresses anti-CCL2 PAGER(TF) in mammalian cells; used with NanoLuc-Arrestin-TEVp (Addgene #125228) and UAS-Firefly Luciferase reporter (Addgene #104840)

   Depositor

   Alice Ting

   Article

   Kalogriopoulos et al Nature. 2024 Dec 4. doi: 10.1038/s41586-

   Insert

   IL2SP-Aro6-8E10-TEVcs-ALFA-KORD(RAA)-LOV-TEVcs-Gal4
   

   Use

   AAV

   Expression

   Mammalian

   Mutation

   V360A/R361A on KORD

   Promoter

   CMV

   Available Since

   Jan. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAAV-anti-mCherry(LaM6) PAGER(TF)

    Plasmid

    #229998

    Purpose

    Expresses anti-mCherry PAGER(TF) (high sensitivity) in mammalian cells; used with NanoLuc-Arrestin-TEVp (Addgene #125228) and UAS-Firefly Luciferase reporter (Addgene #104840)

    Depositor

    Alice Ting

    Article

    Kalogriopoulos et al Nature. 2024 Dec 4. doi: 10.1038/s41586-

    Insert

    IL2SP-Aro6-LaM6-TEVcs-ALFA-KORD(RAA)-LOV-TEVcs-Gal4
    

    Use

    AAV

    Expression

    Mammalian

    Mutation

    V360A/R361A on KORD

    Promoter

    CMV

    Available Since

    Jan. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pGEX-UAP56

    Plasmid

    #157661

    Purpose

    Expresses UAP56 in E.coli cell

    Depositor

    Byung Il Lee

    Article

    Bong et al Nucleic Acids Res. 2020 Jun 19;48(11):63

    Insert

    Spliceosome RNA helicase DDX39B (DDX39B Human)
    

    Tags

    GST tag

    Expression

    Bacterial

    Mutation

    deleted amino acids 1-43

    Available Since

    Aug. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. QSOX1-luciferase

    Plasmid

    #113352

    Purpose

    evaluate FOXA1 DIV motif enhancer activity near 50 kb of QSOX1 gene

    Depositor

    Ralf Jauch

    Article

    Wang et al Nucleic Acids Res. 2018 Jun 20;46(11):54

    Insert

    QSOX1-enhancer (QSOX1 Human)
    

    Use

    Luciferase

    Available Since

    Oct. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. ATP9A-luciferase

    Plasmid

    #113354

    Purpose

    evaluate FOXA1 DIV motif enhancer activity near 50 kb of ATP9A gene

    Depositor

    Ralf Jauch

    Article

    Wang et al Nucleic Acids Res. 2018 Jun 20;46(11):54

    Insert

    ATP9A-enhancer (ATP9A Human)
    

    Use

    Luciferase

    Available Since

    Oct. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. AAV CMV-driven AcrIIA4-2xmiR-122 target sites

    Plasmid

    #120298

    Purpose

    AAV vector for expression of AcrIIA4 with two miR-122 binding sites

    Depositor

    Dominik Niopek

    Article

    Hoffmann et al Nucleic Acids Res. 2019 Apr 15. pii: 545

    Insert

    AcrIIA4-2xmiR-122 binding sites
    

    Use

    AAV and CRISPR

    Tags

    FLAG

    Expression

    Mammalian

    Available Since

    Jan. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

15. pMMDS DEST

    Plasmid

    #206264

    Purpose

    DEST vector for MultiSite Gateway assembly and production of recombinant baculovirus using MultiMate assembly. After LR propagate in Pir+. Works as a CRE-donor in the MultiBac system.

    Depositor

    Imre Berger

    Article

    Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77

    Type

    Empty backbone

    Use

    Cre-donor, multimate/gateway dest vector (multiba…

    Available Since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3-ΔN-RNMT

    Plasmid

    #112708

    Purpose

    Expresses FLAG-NES-RNMT 121-476 D203A ("ΔN-RNMT") in mammalian cells. Overexpression inhibits cytoplasmic cap methylation.

    Depositor

    Daniel Schoenberg

    Article

    Trotman et al Nucleic Acids Res. 2017 Oct 13;45(18):10

    Insert

    RNMT (RNMT Human)
    

    Tags

    FLAG and HIV Rev nuclear export signal (NES)

    Expression

    Mammalian

    Mutation

    deleted amino acids 2-120, mutated active-site D2…

    Promoter

    CMV

    Available Since

    Aug. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pEGFP-RNF8-N-terminus(1-111)

    Plasmid

    #64671

    Purpose

    Expresses only N terminus of RNF8, amino acids 1 to 111

    Depositor

    Marianne Farnebo

    Article

    Henriksson et al Genes Dev. 2014 Dec 15;28(24):2726-38. d

    Insert

    ring finger protein 8 (RNF8 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    containing only N-terminus amino acids 1 to 111

    Promoter

    CMV

    Available Since

    May 21, 2015

    Availability

    Academic Institutions and Nonprofits only

18. Emerald-MannII-N-10

    Plasmid

    #54150

    Depositor

    Michael Davidson

    Article

    Planchon et al Nat Methods. 2011 May;8(5):417-23. doi:

    Insert

    MannII (Man2a1 Mouse)
    

    Tags

    Emerald

    Expression

    Mammalian

    Mutation

    aa 1-112 of MannII aa 1-112 of MannII

    Promoter

    CMV

    Available Since

    July 25, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

19. pEGFP-RNF8-C-terminus

    Plasmid

    #64673

    Purpose

    expresses only C terminus of RNF8, amino acids 402-485

    Depositor

    Marianne Farnebo

    Article

    Henriksson et al Genes Dev. 2014 Dec 15;28(24):2726-38. d

    Insert

    Ring finger protein 8 (RNF8 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    containing only C-terminus amino acids 402 to 485

    Promoter

    CMV

    Available Since

    May 21, 2015

    Availability

    Academic Institutions and Nonprofits only

20. p3xFLAG-CMV-RNF8-N-terminus(1-140)

    Plasmid

    #64675

    Purpose

    expresses only N terminus of RNF8, amino acids 1 to 140

    Depositor

    Marianne Farnebo

    Article

    Henriksson et al Genes Dev. 2014 Dec 15;28(24):2726-38. d

    Insert

    Ring finger protein 8 (RNF8 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    only N terminus amino acids 1-140

    Promoter

    CMV

    Available Since

    May 20, 2015

    Availability

    Academic Institutions and Nonprofits only