We narrowed to 81,643 results for: TRI

1. pIRESpuro-Flag-Pol b(K72A)

   Plasmid

   #23258

   Depositor

   Robert Sobol

   Article

   Tang et al Mol Cancer Res. 2010 Jan;8(1):67-79. doi

   Insert

   DNA polymerase beta (K72A) (POLB Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   K72A

   Available Since

   March 31, 2010

   Availability

   Academic Institutions and Nonprofits only

2. pAG8Ha-SERBP1(149-400)

   Plasmid

   #176516

   Purpose

   Expresses the human C-terminal SERBP1 149-400 construct.

   Depositor

   David Libich

   Article

   Baudin et al Front Mol Biosci. 2021 Sep 24;8:744707.

   Insert

   SERBP1 149-400 (SERBP1 Human)
   

   Tags

   His8 tag, linker, TEV site

   Expression

   Bacterial

   Mutation

   deleted amino acids 1-148 and 401-408

   Promoter

   T7

   Available Since

   June 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3-Venus-cpVenus-FLARE-EKAR-EV

   Plasmid

   #123339

   Purpose

   Yellow-fluorescent homoFRET/anisotropy-based biosensor for monitoring ERK activity. Contains monomeric Venus/cpVenus-E172 homoFRET pair.

   Depositor

   Jin Zhang

   Article

   Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

   Insert

   Venus-cpVenus-FLARE-EKAR-EV
   

   Tags

   6xHIS, T7 tag (gene 10 leader), Venus, Xpress (TM…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGEX 4t1 RARalpha (1-187)

   Plasmid

   #35558

   Depositor

   Jonathan Kurie, Harish Srinivas

   Article

   Srinivas et al Mol Cell Biol. 2005 Feb;25(3):1054-69.

   Insert

   RARalpha (RARA Human)
   

   Tags

   GST

   Expression

   Bacterial

   Mutation

   aa 1-187

   Available Since

   April 5, 2012

   Availability

   Academic Institutions and Nonprofits only

5. pPKm-230

   Plasmid

   #90499

   Purpose

   pSIN - EF-1alpha - PIF3 - MTAD - IRES - PhyB - GBD, dual vector of PIF3-MTAD under EF-1 alpha promoter and PhyB-DBD under IRES promoter; See growth conditions below.

   Depositor

   Todd Coleman

   Article

   Kyriakakis et al ACS Synth Biol. 2018 Feb 16;7(2):706-717

   Insert

   mito-tFD and mito-tFNR
   

   Use

   Lentiviral

   Available Since

   June 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pIRESneo-MPG(N169D)

   Plasmid

   #23259

   Depositor

   Robert Sobol

   Article

   Tang et al Mol Cancer Res. 2010 Jan;8(1):67-79. doi

   Insert

   N-methylpurine DNA glycosylase (N169D) (MPG Human)
   

   Expression

   Mammalian

   Mutation

   N169D

   Available Since

   March 31, 2010

   Availability

   Academic Institutions and Nonprofits only

7. pAAV-hGRM1-RFP

   Plasmid

   #22920

   Depositor

   Edward Callaway

   Article

   Nathanson et al Front Neural Circuits. 2009 Nov 9;3:19.

   Insert

   human receptor metabotropic 1 promoter driving DsRedExpress (GRM1 Human)
   

   Use

   AAV

   Expression

   Mammalian

   Available Since

   May 25, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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8. K15-TK/pGL3

   Plasmid

   #44267

   Depositor

   George Cotsarelis

   Article

   Ito et al Nat Med. 2005 Dec;11(12):1351-4. Epub 20

   Insert

   K15 promoter (-4.8) (Krt15 Mouse)
   

   Use

   Mouse Targeting; Thymidine kinase

   Tags

   HSV-1 TK

   Expression

   Mammalian

   Mutation

   Contains the murine K15 promoter (-4.8) fragment

   Promoter

   murine K15 promoter (-4.8) fragment

   Available Since

   April 2, 2013

   Availability

   Academic Institutions and Nonprofits only

9. pAAV-mA93-RFP

   Plasmid

   #22918

   Depositor

   Edward Callaway

   Article

   Nathanson et al Front Neural Circuits. 2009 Nov 9;3:19.

   Insert

   mouse riken gene A930038C07Rik promoter driving DsRedExpress (A930038C07Rik Mouse)
   

   Use

   AAV

   Expression

   Mammalian

   Available Since

   Aug. 26, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMT-zic2a-cytoBirA-2A-mCherry_Ras

    Plasmid

    #80064

    Purpose

    Zic2a promoter driving HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences

    Depositor

    Tatjana Sauka-Spengler

    Article

    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

    Insert

    Biotin ligase (BirA), a Thosea asigna virus peptide (2A), and mCherry protein
    

    Use

    Zebrafish biotagging

    Tags

    3x HA

    Expression

    Bacterial

    Promoter

    Zic2a enhancer driving c-fos promoter

    Available Since

    Oct. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMT-ubb-NLS-BirA-2a-mCherry

    Plasmid

    #79886

    Purpose

    Ubiquitin promoter driving HA-tagged biotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein; flanked by Tol2 sequences

    Depositor

    Tatjana Sauka-Spengler

    Article

    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

    Insert

    Biotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein
    

    Tags

    3x HA

    Expression

    Bacterial

    Promoter

    Ubiquitin

    Available Since

    Oct. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pPN007

    Plasmid

    #91575

    Purpose

    Express sgRNA targeting human BCL11A

    Depositor

    Lindy Barrett

    Article

    Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

    Insert

    BCL11A (BCL11A Human)
    

    Use

    CRISPR

    Promoter

    U6

    Available Since

    Oct. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMT-MCS-NLS-BirA-2A-mCherry_Ras

    Plasmid

    #80059

    Purpose

    MCS for cloning promoter to drive HA-tagged biotin ligase (BirA) with NLS, a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences

    Depositor

    Tatjana Sauka-Spengler

    Article

    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

    Insert

    Biotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein
    

    Use

    Zebrafish biotagging

    Tags

    3x HA

    Expression

    Bacterial

    Available Since

    Oct. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA3-Lyn-Venus-cpVenus-FLARE-AKAR

    Plasmid

    #123337

    Purpose

    Plasma membrane-targeted yellow-fluorescent homoFRET/anisotropy-based biosensor for monitoring Protein Kinase A activity.

    Depositor

    Jin Zhang

    Article

    Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

    Insert

    Lyn-Venus-cpVenus-FLARE-AKAR
    

    Tags

    N-terminal targeting sequence from Lyn kinase, Ve…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGEX-6P-1-INTS3-C

    Plasmid

    #128417

    Purpose

    Express INTS3 C-termini (514-1042 aa) in E. coli with a GST tag (N-terminal)

    Depositor

    Yuliang Wu

    Article

    Vidhyasagar et al Biochem J. 2018 Jan 2;475(1):45-60. doi:

    Insert

    INTS3 (INTS3 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    INTS3 C-terminal region (514-1042 aa)

    Promoter

    Tac promoter

    Available Since

    Aug. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBabe JunD-Venus puro

    Plasmid

    #58496

    Purpose

    mammalian expression of Venus-tagged mouse JunD

    Depositor

    Kevin Janes

    Article

    Wang et al Nat Cell Biol. 2014 Apr;16(4):345-56. do

    Insert

    JunD (Jund Mouse)
    

    Tags

    Venus

    Expression

    Mammalian

    Available Since

    Aug. 12, 2014

    Availability

    Academic Institutions and Nonprofits only

17. pcDNA3-Venus-cpVenus-FLARE-CKAR

    Plasmid

    #123343

    Purpose

    Yellow-fluorescent homoFRET/anisotropy-based biosensor for monitoring Protein Kinase C activity. Contains monomeric Venus/cpVenus-E172 homoFRET pair.

    Depositor

    Jin Zhang

    Article

    Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

    Insert

    Venus-cpVenus-FLARE-CKAR
    

    Tags

    6xHIS, T7 tag (gene 10 leader), Venus, Xpress (TM…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. HOXB5 (human) FLAG-MSCV

    Plasmid

    #8532

    Depositor

    Corey Largman

    Article

    Shen et al Proc Natl Acad Sci U S A 1989 Nov;86(21)

    Insert

    HOXB5 (HOXB5 Human)
    

    Use

    Retroviral

    Tags

    FLAG and IRES-GFP

    Expression

    Mammalian

    Mutation

    contains improved ribosomal binding site and FLAG…

    Available Since

    May 30, 2006

    Availability

    Academic Institutions and Nonprofits only

19. pRK5 GST MetaP2

    Plasmid

    #112750

    Purpose

    express HA-GST-FRB-MetaP2

    Depositor

    Roberto Zoncu

    Article

    Lawrence et al Nat Cell Biol. 2018 Sep;20(9):1052-1063.

    Insert

    HA-GST-FRB-MetaP2 (METAP2 Human)
    

    Tags

    GST

    Expression

    Mammalian

    Available Since

    Oct. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCS2+ cMyc-oDam_f_GFPoNLS

    Plasmid

    #85818

    Purpose

    iDamID plasmid. To express transiently the c-Myc-tagged and optimized E. coli Dam adenine methyltransferase fused to the nuclear-localized mmGFP via flexylinker.

    Depositor

    Joachim Wittbrodt

    Article

    Gutierrez-Triana et al Development. 2016 Oct 5. pii: dev.139261

    Insert

    cMyc oDam_f_GFPoNLS
    

    Tags

    cMyc and oNLS (optimized nuclear localization sig…

    Expression

    Bacterial, Insect, Mammalia…

    Mutation

    L122A. Codon optimization compatible to work in M…

    Available Since

    Jan. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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