164,892 results

1. pNestin-EGFP

   Plasmid

   #38777

   Depositor

   Wei Cui

   Article

   Noisa et al Stem Cell Rev. 2010 Sep;6(3):438-49.

   Insert

   nestin 2nd intron enhancer with TK mini promoter (NES Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 15, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMAH-EcaYRS

   Plasmid

   #153558

   Purpose

   Amber suppression for incorporating 3-aminotyrosine to proteins in mammalian cells

   Depositor

   Huiwang Ai

   Article

   Zhang et al Nat Chem Biol. 2020 Sep 14. pii: 10.1038

   Inserts

   EcaYRS
   amber suppression tRNA (multiple copies)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. U6-sgH11-eCas9-T2A-BlastR

   Plasmid

   #172492

   Purpose

   For CRISPR-assisted HDR, Hipp11 locus

   Depositor

   Tyler Jacks

   Article

   Freed-Pastor et al Cancer Cell. 2021 Oct 11;39(10):1342-136

   Insert

   , BlastR and sgRNA targeting the Hipp11 (H11) safe harbor locus
   

   Use

   CRISPR, Lentiviral, and Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGEL685

   Plasmid

   #205592

   Purpose

   NEPE for prime editing in rice

   Depositor

   Yong Zhang

   Article

   Zhong et al Plant Commun. 2024 May 14:100976. doi: 1

   Type

   Empty backbone

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   Promoter

   ZmUbi

   Available since

   May 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEntry_SARS-CoV-2-nsp16

   Plasmid

   #168876

   Purpose

   Gateway-compatible Entry vector

   Depositor

   Pascal Falter-Braun

   Article

   Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

   Insert

   SARS-CoV-2-nsp16 (ORF1ab )
   

   Use

   Gateway-compatible entry vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. NylCK-TS_pET28a

   Plasmid

   #215423

   Purpose

   Expression construct for NylCK-TS gene, codon optimized for expression in E. coli

   Depositor

   Gregg Beckham

   Article

   Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

   Insert

   NylCK-TS
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   S111G/A137L

   Promoter

   Available since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pX330A-FBL/PITCh

   Plasmid

   #63671

   Purpose

   Expresses Cas9 nuclease, FBL-specific gRNA, and the PITCh-gRNA

   Depositor

   Takashi Yamamoto

   Article

   Sakuma et al Nat Protoc. 2016 Jan;11(1):118-33. doi:

   Insert

   humanized S. pyogenes Cas9
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CBh

   Available since

   Dec. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

8. pET3a-hUBA1

   Plasmid

   #63571

   Purpose

   Expression of human UBA1 tagged with His

   Depositor

   Titia Sixma

   Article

   Pichler et al Nat Struct Mol Biol. 2005 Mar;12(3):264-

   Insert

   UBA1 (UBA1 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Isoform 2 (1018 amino acids)

   Promoter

   T7

   Available since

   March 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEGFP-N1-FLAG

   Plasmid

   #60360

   Purpose

   Plasmid for cloning and expression of FLAG-GFP tagged proteins (C-terminal tag). Confers resistance to G418.

   Depositor

   Patrick Calsou, Sébastien Britton

   Article

   Britton et al Nucleic Acids Res. 2014 Oct 1;42(14):904

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Tags

   EGFP and FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. Lenti-EF1alpha-VP64-dCas9-p300_Blast

    Plasmid

    #192654

    Purpose

    3rd generation lenti vector encoding VP64-dCas9-p300 with 2A Blast resistance marker

    Depositor

    Jeffrey Miner

    Article

    Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

    Insert

    VP64-dCas9-p300
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    D10A; H840A in Cas9

    Promoter

    EF1a

    Available since

    March 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. trPAGmEos3.2

    Plasmid

    #203752

    Purpose

    Encodes the transmembrane domain from PAG/CSK, including its 2 palmitoylation sites, with mEos3.2 fused on the C terminus. Used as a fluorescent plasma membrane probe.

    Depositor

    Sarah Veatch

    Article

    Shelby et al Nat Chem Biol. 2023 Jun;19(6):750-758. d

    Insert

    trPAG (PAG1 Human)
    

    Use

    Tags

    mEos3.2

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. mPlum-pBAD

    Plasmid

    #54564

    Purpose

    Localization: Protein Expression Vector , Excitation: 590, Emission: 649

    Depositor

    Roger Tsien, Michael Davidson

    Article

    Wang et al Proc Natl Acad Sci U S A. 2004 Nov 30;10

    Type

    Empty backbone

    Use

    Tags

    6xHis and mPlum

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pENTR-MBD1

    Plasmid

    #47057

    Purpose

    Expresses methyl-CpG binding domain protein 1

    Depositor

    Huda Zoghbi

    Article

    Sakai et al Sci Transl Med. 2011 Jun 8;3(86):86ra49.

    Insert

    Methyl-CpG Binding Domain Protein 1 (MBD1 Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 29, 2013

    Availability

    Academic Institutions and Nonprofits only

14. pAAV-pCMV-dSaCas9-VP64-pU6-sgRNA

    Plasmid

    #158990

    Purpose

    Vector G encodes pAAV-pCMV-dSaCas9-VP64-spA-pU6-sgRNA (BsaI) transgenes for AAV packaging and expression of CRISPR activator in mammalian cells

    Depositor

    Chung Tin

    Article

    Lau et al Mol Ther Nucleic Acids. 2019 Jun 7;16:63

    Insert

    dSaCas9-VP64
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pCMV

    Available since

    Aug. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMGE-T7

    Plasmid

    #153021

    Purpose

    Single-plasmid multi-gene expression system. Each genes under the control of a T7 and the lacO promoter sequences.

    Depositor

    Vito Valiante

    Article

    Kufs et al ACS Synth Biol. 2020 Jun 11. doi: 10.102

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLVX-puro-cOVA

    Plasmid

    #135073

    Purpose

    Expresses chicken cytoplasmic ovalbumin in lentiviral vector with puromycin resistance

    Depositor

    Maria Castro

    Insert

    cOVA (OVAL Chicken)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRosetta

    Plasmid

    #59700

    Purpose

    Lentiviral vector for use as a standard for titering experiments when trying to compare vectors with different selection markers or to determine appropriate antibiotic conditions.

    Depositor

    David Root, John Doench

    Inserts

    Puro
    BlastR
    EGFP
    

    Use

    Lentiviral

    Tags

    2A

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHes1-DsRed

    Plasmid

    #13767

    Depositor

    Connie Cepko

    Article

    Matsuda et al Proc Natl Acad Sci U S A. 2007 Jan 16;10

    Insert

    Hes1 promoter
    

    Use

    Tags

    DsRed2

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 20, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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19. SynTagMA_post

    Plasmid

    #119736

    Purpose

    Synaptic Tag for Mapping Activity

    Depositor

    Thomas Oertner

    Article

    Perez-Alvarez et al Nat Commun. 2020 May 18;11(1):2464. doi:

    Insert

    SynTagMA
    

    Use

    AAV

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn-1

    Available since

    Feb. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV_BiSSTe4_GCaMP6f

    Plasmid

    #213947

    Purpose

    AAV construct expressing GCaMP6f driven by SST interneuron-targeting enhancer. Alias: pAAV_WDS0004_GCaMP6f

    Depositor

    Gordon Fishell

    Article

    Furlanis et al Neuron. 2025 May 21;113(10):1525-1547.e1

    Insert

    GCaMP6f
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge