We narrowed to 12,189 results for: LEA

Showing: 6241 - 6260 of 12189 results

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pLKO_C.HSV.S_mCherry-NLS

Plasmid

#178245

Purpose

Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.

Depositor

Brian Brown

Publication

Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00

Insert

nPC Tagged mCherry-NLS

Use

Lentiviral

Tags

C.HSV.S and Nuclear Localization Signal

Promoter

eF1a

Available since

Jan. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pLKO_C.HA.VSVg_mCherry-NLS

Plasmid

#178241

Purpose

Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.

Depositor

Brian Brown

Publication

Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00

Insert

nPC Tagged mCherry-NLS

Use

Lentiviral

Tags

C.HA.VSVg and Nuclear Localization Signal

Promoter

eF1a

Available since

Jan. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pLKO_C.HA.S_mCherry-NLS

Plasmid

#178240

Purpose

Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.

Depositor

Brian Brown

Publication

Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00

Insert

nPC Tagged mCherry-NLS

Use

Lentiviral

Tags

C.HA.S and Nuclear Localization Signal

Promoter

eF1a

Available since

Jan. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pLKO_C.HA.HSV_mCherry-NLS

Plasmid

#178237

Purpose

Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.

Depositor

Brian Brown

Publication

Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00

Insert

nPC Tagged mCherry-NLS

Use

Lentiviral

Tags

C.HA.HSV and Nuclear Localization Signal

Promoter

eF1a

Available since

Jan. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pJH807_pJH_LldR_Pcon-sfGFP.2TAG

Plasmid

#163738

Purpose

Constitutive expression of green fluorescent protein encoding two stop codons

Depositor

Matthew Bennett

Publication

Ho et al Nucleic Acids Res. 2021 Mar 18;49(5):e25

Insert

lldr gfp.2TAG

Expression

Bacterial

Mutation

gfp.N39TAG,Y135TAG

Available since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pJH27_pBad_AraC_Para-sfGFP.2TAG

Plasmid

#163722

Purpose

Arabinose-inducible expression of green fluorescent protein encoding two amber codons

Depositor

Matthew Bennett

Publication

Ho et al Nucleic Acids Res. 2021 Mar 18;49(5):e25

Insert

araC gfp.2TAG

Expression

Bacterial

Mutation

gfp.N39TAG,Y151TAG

Available since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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TetO::EGFP-hMeikin

Plasmid

#174705

Purpose

Doxycycline inducible expression of human Meikin

Depositor

Iain Cheeseman

Publication

Maier et al Dev Cell. 2021 Aug 9;56(15):2192-2206.e8

Insert

Meikin

Tags

EGFP

Expression

Mammalian

Available since

Sept. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pJCC_083 Cas12i1

Plasmid

#171670

Purpose

For bacterial expression of Cas12i1 with an N-terminal His-MBP tag

Depositor

Jennifer Doudna

Publication

Cofsky et al Elife. 2020 Jun 10;9. pii: 55143. doi: 1

Insert

Cas12i1

Tags

10X His, TEV protease cleavage site, and maltose-…

Expression

Bacterial

Available since

June 16, 2021

Availability

Academic Institutions and Nonprofits only

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His-SUMO-flag-hDDI2_pET11a

Plasmid

#169121

Purpose

expression of protein

Depositor

Barbara Dirac-Svejstrup

Publication

Dirac-Svejstrup et al Mol Cell. 2020 Jul 16;79(2):332-341.e7.

Insert

hDDI (DDI2 Human)

Expression

Bacterial

Available since

May 13, 2021

Availability

Academic Institutions and Nonprofits only

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Nup54 no FG-mEGFP494(0)

Plasmid

#164269

Purpose

Mammalian expression of Nup54 without the FG-Nup domain, with mEGFP (missing the first 5 amino acids) at amino acid position 494 with a rigid alpha helix of 5 amino acids at the carboxyl end of mEGFP

Depositor

Sanford Simon

Publication

Pulupa et al Elife. 2020 Dec 21;9. pii: 60654. doi: 1

Insert

Nup54 (NUP54 Human)

Tags

mEGFP

Expression

Mammalian

Mutation

deleted the FG region of Nup54

Promoter

CMV

Available since

Feb. 23, 2021

Availability

Academic Institutions and Nonprofits only

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fADL-1e-flag-linker-p3

Plasmid

#139441

Purpose

For cloning and subsequent expression of peptide fragments fused with pIII protein as a part of fd filamentous bacteriophage.

Depositor

Alexander Gabibov

Publication

Ishina et al Int J Mol Sci. 2020 Aug 29;21(17). pii:

Insert

linker with pelB-leader, cloning site, flag-tag and p3

Expression

Bacterial

Available since

Aug. 31, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pTEI134

Plasmid

#126071

Purpose

Vector for expression of plastid targeted YFP1-10 (T203Y) in plant cell

Depositor

Ralf-Bernd Klösgen

Publication

Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs

Insert

Chloroplastic FNR transit peptide

Use

Binary

Tags

YFP1-10

Expression

Plant

Available since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pEPOR0SP0007

Plasmid

#117534

Purpose

Level0 Golden Gate part: CDS, LbCas12a

Depositor

Nicola Patron

Publication

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

LbCas12a (with stop codon)

Use

CRISPR

Available since

Nov. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pJOG140

Plasmid

#105399

Purpose

Synthetic biology

Depositor

Johannes Stuttmann

Publication

Gantner et al PLoS One. 2018 May 30;13(5):e0197185. do

Insert

PKI nuclear export signal

Use

Synthetic Biology

Mutation

BsaI/ BpiI restriction sites removed

Available since

Aug. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pJOG141

Plasmid

#105400

Purpose

Synthetic biology

Depositor

Johannes Stuttmann

Publication

Gantner et al PLoS One. 2018 May 30;13(5):e0197185. do

Insert

SV40 nuclear localization signal

Use

Synthetic Biology

Mutation

BsaI/ BpiI restriction sites removed

Available since

Aug. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pJOG824

Plasmid

#105401

Purpose

Synthetic biology

Depositor

Johannes Stuttmann

Publication

Gantner et al PLoS One. 2018 May 30;13(5):e0197185. do

Insert

SV40 nuclear localization signal

Use

Synthetic Biology

Mutation

BsaI/ BpiI restriction sites removed

Available since

March 27, 2018

Availability

Academic Institutions and Nonprofits only

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pEANHISECArdB

Plasmid

#96967

Purpose

Expression of E. coli ArdB protein wirh an N-terminal TEV protease cleavable His tag

Depositor

Huanting Liu, James Naismith

Publication

Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

Insert

E. coli ArdB

Tags

N-ter TEV cleavable 6His

Expression

Bacterial

Mutation

none

Promoter

T7

Available since

Aug. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pET21a - EtfA

Plasmid

#89616

Purpose

Expresses recombinant 6HIS tagged T. cruzi electron transfer flavoprotein A in E. coli upon IPTG induction

Depositor

Judy Lieberman

Publication

Dotiwala et al Nat Med. 2016 Feb;22(2):210-6. doi: 10.1

Insert

Electron transport flavoprotein alpha subunit

Tags

6HIS and T7 tag (gene 10 leader)

Expression

Bacterial

Available since

May 22, 2017

Availability

Academic Institutions and Nonprofits only

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pET21a - EtfB

Plasmid

#89617

Purpose

Expresses recombinant 6HIS tagged T. cruzi electron transfer flavoprotein B in E. coli upon IPTG induction

Depositor

Judy Lieberman

Publication

Dotiwala et al Nat Med. 2016 Feb;22(2):210-6. doi: 10.1

Insert

electron-transfer-flavoprotein, alpha polypeptide

Tags

6HIS and T7 tag (gene 10 leader)

Expression

Bacterial

Available since

May 22, 2017

Availability

Academic Institutions and Nonprofits only

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sgGCAT_2

Plasmid

#72885

Purpose

CRISPR-Cas9 induced gene disruption

Depositor

David Sabatini

Publication

Kim et al Nature. 2015 Apr 16;520(7547):363-7. doi

Insert

sgGCAT

Use

Lentiviral

Promoter

CMV

Available since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only