We narrowed to 9,182 results for: mel

1. pLJM1 FLCN (R164A) FLAG GFP

   Plasmid

   #164980

   Purpose

   Lentiviral vector for FLCN expression in mammalian cells

   Depositor

   Roberto Zoncu

   Article

   Lawrence et al Science. 2019 Nov 22;366(6468):971-977.

   Insert

   FLCN (FLCN Human)
   

   Use

   Lentiviral

   Tags

   1xFLAG-GFP

   Expression

   Mammalian

   Mutation

   R164A

   Promoter

   UBC

   Available Since

   March 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. FCK-Chronos-GFP

   Plasmid

   #80902

   Purpose

   Lenti-mediated expression of Chronos-GFP under the CaMKII promoter.

   Depositor

   Edward Boyden

   Article

   Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

   Insert

   Chronos-GFP
   

   Use

   Lentiviral

   Tags

   GFP

   Expression

   Mammalian

   Promoter

   CaMKII

   Available Since

   March 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLX304-MARK3_T211A

   Plasmid

   #107236

   Purpose

   Lentiviral gene expression vector for phospho-MARK3_T221A expression

   Depositor

   Alex Kentsis

   Article

   Brown et al Cancer Discov. 2018 Apr;8(4):478-497. do

   Insert

   MARK3
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   May 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pYFP-ErbB2ΔC990

   Plasmid

   #66946

   Purpose

   Mammalian expression of rat ErbB2 ΔC990 tagged with YFP

   Depositor

   Mads Lerdrup, Bo van_Deurs

   Article

   Lerdrup et al Mol Biol Cell. 2007 Sep;18(9):3656-66. E

   Insert

   ErbB2 (Erbb2 Rat)
   

   Tags

   6xHis, V5 Tag, and YFP

   Expression

   Mammalian

   Mutation

   Deletion of C990. (see depositor comment below on…

   Promoter

   CMV

   Available Since

   Sept. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

5. Gal4-entry+NLS

   Plasmid

   #128013

   Purpose

   Vector containing the Gal4-entry expression cassette with SV40 NLS and a separate expression cassette driving mCherry via the traffic jam enhancer

   Depositor

   Julius Brennecke

   Article

   Batki et al Nat Struct Mol Biol. 2019 Aug;26(8):720-

   Insert

   Gal4 DBD-SV40NLS-3x-Flag
   

   Use

   Gal4 entry vector with nls to generate tethering …

   Tags

   Gal4-DBD-SV40NLS-3xFlag

   Available Since

   Aug. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pYFP-ErbB2ΔC776

   Plasmid

   #66947

   Purpose

   Mammalian expression of rat ErbB2 ΔC776 tagged with YFP

   Depositor

   Mads Lerdrup, Bo van_Deurs

   Article

   Lerdrup et al Mol Biol Cell. 2007 Sep;18(9):3656-66. E

   Insert

   ErbB2 (Erbb2 Rat)
   

   Tags

   6xHis, V5 Tag, and YFP

   Expression

   Mammalian

   Mutation

   Deletion of C776 (see depositor comment below on …

   Promoter

   CMV

   Available Since

   Sept. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

7. pLJM1 FLCN (F118D) FLAG GFP

   Plasmid

   #164979

   Purpose

   Lentiviral vector for FLCN expression in mammalian cells

   Depositor

   Roberto Zoncu

   Article

   Lawrence et al Science. 2019 Nov 22;366(6468):971-977.

   Insert

   FLCN (FLCN Human)
   

   Use

   Lentiviral

   Tags

   1xFLAG-GFP

   Expression

   Mammalian

   Mutation

   F118D

   Promoter

   UBC

   Available Since

   March 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. FLAG-MED27

   Plasmid

   #15424

   Depositor

   Joan Conaway, Ronald Conaway

   Article

   Sato et al J Biol Chem. 2003 Apr 25. 278(17):15123-

   Insert

   MED27 (MED27 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   N/A

   Available Since

   Aug. 15, 2007

   Availability

   Academic Institutions and Nonprofits only

9. FLAG-MED21

   Plasmid

   #15422

   Depositor

   Joan Conaway, Ronald Conaway

   Article

   Sato et al J Biol Chem. 2003 Apr 25. 278(17):15123-

   Insert

   MED21 (MED21 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   N/A

   Available Since

   Aug. 15, 2007

   Availability

   Academic Institutions and Nonprofits only

10. pET28a(His10-PD-1cyto)

    Plasmid

    #177896

    Purpose

    E coli expression of His10 tag fused to human PD-1 Intracellular Domain

    Depositor

    Ron Vale

    Article

    Hui et al Science. 2017 Mar 31;355(6332):1428-1433

    Insert

    PD-1 (aa 194-288 only) (PDCD1 Human)
    

    Tags

    10xHis-Thrombin cut site-T7 tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pFastBac(His10-Shp2)

    Plasmid

    #177899

    Purpose

    Baculo expression of His10 tag fused to human Shp2

    Depositor

    Ron Vale

    Article

    Hui et al Science. 2017 Mar 31;355(6332):1428-1433

    Insert

    Shp2 (PTPN11 Human)
    

    Tags

    10xHis-TEV-SNAP tag

    Expression

    Insect

    Promoter

    Polyhedrin

    Available Since

    April 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. prk5-MFSD12-TEV-3xFLAG-3xHA PM mutant

    Plasmid

    #149507

    Purpose

    Transient expression of human MFSD12 with recoded nucleotide sequence. Dileucine motif LL256-257 has been mutated to AA.

    Depositor

    David Sabatini

    Article

    Adelmann et al Nature. 2020 Nov 18. pii: 10.1038/s41586

    Insert

    MFSD12 (MFSD12 Human)
    

    Use

    Transient expression

    Tags

    TEV-3XHA-3XFLAG

    Expression

    Mammalian

    Mutation

    Codon Optimized Sequence, p.LL256-257AA

    Promoter

    CMV

    Available Since

    Nov. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. SopB-EGFP

    Plasmid

    #183655

    Purpose

    C-terminally tagged Salmonella Typhimurium SopB for mammalian expression

    Depositor

    Sergio Grinstein

    Article

    Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

    Insert

    sopB (sopB Salmonella enterica serovar Typhimurium)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-Syn-CsChR-GFP

    Plasmid

    #58841

    Purpose

    AAV expression of CsChR-GFP under the Syn promoter

    Depositor

    Edward Boyden

    Article

    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

    Insert

    CsChR-GFP
    

    Use

    AAV

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    Syn

    Available Since

    Aug. 14, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLEX307-HCoV-NL63-3CL-C144A

    Plasmid

    #160287

    Purpose

    Allows for constituitive mammalian expression of the HCoV-NL63 3CL protease with C144A mutation via transfection or lentivirus

    Depositor

    Alejandro Chavez

    Article

    Resnick et al J Virol. 2021 Jun 24;95(14):e0237420. do

    Insert

    3CL Protease
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    C144A

    Promoter

    EF1a

    Available Since

    Dec. 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLEX307-SARS-CoV-3CL-C145A

    Plasmid

    #160281

    Purpose

    Allows for constituitive mammalian expression of the SARS-CoV 3CL protease with C145A mutation via transfection or lentivirus

    Depositor

    Alejandro Chavez

    Article

    Resnick et al J Virol. 2021 Jun 24;95(14):e0237420. do

    Insert

    3CL Protease
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    C145A

    Promoter

    EF1a

    Available Since

    Oct. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pXPR_BRD111

    Plasmid

    #78166

    Purpose

    Expresses Cas9 with the pLX311 backbone

    Depositor

    William Hahn

    Article

    Hong et al Nat Commun. 2016 Jun 22;7:11987. doi: 10

    Insert

    Cas9
    

    Use

    Lentiviral

    Tags

    3xFLAG and NLS

    Available Since

    June 22, 2016

    Availability

    Academic Institutions and Nonprofits only

18. pX131

    Plasmid

    #167149

    Purpose

    MoClo-compatible Level 0-SP promoterless vector encoding Aspergillus nidulans 4'-phosphopantetheinyl transferase NpgA codon-optimised for expression in Nicotiana benthamiana and Pichia pastoris

    Depositor

    Karen Sarkisyan

    Article

    Mitiouchkina et al Nat Biotechnol. 2020 Apr 27. pii: 10.103

    Insert

    4'-phosphopantetheinyl transferase, npgA
    

    Use

    Synthetic Biology

    Available Since

    June 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pESC-LEU-SopB

    Plasmid

    #183670

    Purpose

    Inducible Salmonella Typhimurium SopB for expression in yeast

    Depositor

    Sergio Grinstein

    Article

    Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

    Insert

    sopB (sopB Salmonella enterica serovar Typhimurium)
    

    Expression

    Yeast

    Promoter

    GAL1

    Available Since

    May 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pESC-Leu-SopB(C460S)

    Plasmid

    #183671

    Purpose

    Inducible Salmonella Typhimurium SopB (catalytically inactive) for expression in yeast

    Depositor

    Sergio Grinstein

    Article

    Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

    Insert

    sopB (sopB Salmonella enterica serovar Typhimurium)
    

    Expression

    Yeast

    Mutation

    C460S

    Promoter

    GAL1

    Available Since

    May 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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