We narrowed to 9,310 results for: yeast expression

1. pET28b(+)_rPD-L1nb

   Plasmid

   #226715

   Purpose

   Heterologous expression and purification of recombinant PD-L1 nanobody (rPD-L1nb)

   Depositor

   Gautam Dantas

   Article

   Rebeck et al Cell Chem Biol. 2024 Nov 16:S2451-9456(2

   Insert

   Recombinant PD-L1 nanobody
   

   Tags

   HA tag and Histidine tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Dec. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pETDuet-1 TIM9,10

   Plasmid

   #170280

   Purpose

   Expresses both yeast Tim9 and yeast Tim10 (the latter with cleavable N-ter His-tag), codon-optimized for E. coli production

   Depositor

   Paul Schanda

   Article

   Weinhaupl et al Cell. 2018 Nov 15;175(5):1365-1379.e25.

   Insert

   mitochondrial Tim9 chaperone subunit and Tim10 (with N-ter His tag) (TIM9, TIM10 Budding Yeast)
   

   Expression

   Bacterial

   Promoter

   Tim9: via NdeI/XhoI into the MCS2; Tim10: via Bam…

   Available Since

   June 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pETcon_SARS-CoV-2_RBD

   Plasmid

   #166782

   Purpose

   Yeast surface display vector, expressing the SARS-2 RBD, 201aa construct

   Depositor

   Jesse Bloom

   Article

   Starr et al Cell. 2020 Sep 3;182(5):1295-1310.e20. d

   Insert

   Spike receptor binding domain (RBD) from SARS-CoV-2 (S )
   

   Expression

   Yeast

   Available Since

   March 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRS313_ATP3T911A

   Plasmid

   #120254

   Purpose

   Expression of ATP3-6 in yeast

   Depositor

   Markus Ralser

   Article

   Vowinckel et al Nat Metab. 2021 Nov;3(11):1521-1535. doi

   Insert

   ATP3 (ATP3 Budding Yeast)
   

   Expression

   Yeast

   Mutation

   T911 changed to A; Isoleucine 304 changed to Aspa…

   Promoter

   ATP3

   Available Since

   April 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAG416GALL-ccdB-6stop

   Plasmid

   #203511

   Purpose

   Gateway-compatible destination vector for yeast expression with GALL promoter/6stop mutation/URA3 marker

   Depositor

   Alejandro Chavez

   Article

   Hong et al Mol Syst Biol. 2025 Feb;21(2):158-172. d

   Type

   Empty backbone

   Expression

   Yeast

   Available Since

   July 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAG416GAL10-ccdB-6stop

   Plasmid

   #203512

   Purpose

   Gateway-compatible destination vector for yeast expression with GAL10 promoter/6stop mutation/URA3 marker

   Depositor

   Alejandro Chavez

   Article

   Hong et al Mol Syst Biol. 2025 Feb;21(2):158-172. d

   Type

   Empty backbone

   Expression

   Yeast

   Available Since

   July 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pESC-Leu-SopB(C460S)

   Plasmid

   #183671

   Purpose

   Inducible Salmonella Typhimurium SopB (catalytically inactive) for expression in yeast

   Depositor

   Sergio Grinstein

   Article

   Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

   Insert

   sopB (sopB Salmonella enterica serovar Typhimurium)
   

   Expression

   Yeast

   Mutation

   C460S

   Promoter

   GAL1

   Available Since

   May 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDUAL-Pnmt1-Ost4-GFP-MTn

   Plasmid

   #154369

   Purpose

   Yeast expression of Ost4

   Depositor

   Wanzhong He

   Article

   Jiang et al Nat Methods. 2020 Sep;17(9):937-946. doi

   Insert

   Ost4 (ost4 Fission Yeast)
   

   Expression

   Yeast

   Available Since

   July 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDUAL-P41nmt1-Nup124-GFP-MTn

   Plasmid

   #154365

   Purpose

   Yeast expression of Nup124

   Depositor

   Wanzhong He

   Article

   Jiang et al Nat Methods. 2020 Sep;17(9):937-946. doi

   Insert

   Nup124 (nup124 Fission Yeast)
   

   Expression

   Yeast

   Mutation

   See depositor comments below

   Available Since

   Aug. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pACT-HAB1

    Plasmid

    #241276

    Purpose

    Yeast two hybrid vector expressing AD-HAB1

    Depositor

    Sean Cutler

    Article

    Park et al Science. 2009 May 22;324(5930):1068-71.

    Insert

    HAB1 (HAB1 Mustard Weed)
    

    Expression

    Yeast

    Mutation

    NA

    Available Since

    Sept. 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pJRoC30-LaccID

    Plasmid

    #234652

    Purpose

    Expresses LaccID evolved variant in yeast

    Depositor

    Alice Ting, Miguel Alcalde Galeote

    Article

    Lee et al Nat Chem Biol. 2025 Aug 1. doi: 10.1038/

    Insert

    LaccID
    

    Tags

    HRV 3C protease site-GSG linker-8xHis tag

    Expression

    Yeast

    Mutation

    V11A, Q45R, N74S, R121S, I126V, D255G, L300V

    Promoter

    Gal1p

    Available Since

    Aug. 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pKG-GW2-NgBR

    Plasmid

    #203165

    Purpose

    Yeast expression vector for hNUS1

    Depositor

    William Sessa

    Article

    Park et al Cell Metab. 2014 Sep 2;20(3):448-57. doi

    Insert

    NUS1 (NUS1 Human)
    

    Expression

    Yeast

    Promoter

    TDH3

    Available Since

    Aug. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pKG-GW2-NgBR R290H

    Plasmid

    #203167

    Purpose

    Yeast expression vector for hNUS1 R290H

    Depositor

    William Sessa

    Article

    Park et al Cell Metab. 2014 Sep 2;20(3):448-57. doi

    Insert

    NUS1 (NUS1 Human)
    

    Expression

    Yeast

    Mutation

    R290H

    Promoter

    TDH3

    Available Since

    Aug. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pESC-LEU-IpgD(C439S)

    Plasmid

    #183673

    Purpose

    Inducible Shigella flexneri IpgD (catalytically inactive) for expression in yeast

    Depositor

    Sergio Grinstein

    Article

    Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

    Insert

    IpgD
    

    Expression

    Yeast

    Mutation

    C439S

    Promoter

    GAL1

    Available Since

    May 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGG442

    Plasmid

    #165616

    Purpose

    Vector for expression of SpCas9-Zif268 in yeast after assembly with PID fragments: TEF1p-SpCas9::KanR-1xNLS-3xHA-1xNLS-Zif268-1xNLS-CYC1t (KanR cassette in place of the PID)

    Depositor

    Marcus Noyes

    Article

    Goldberg et al Nat Commun. 2021 Jan 13;12(1):349. doi:

    Insert

    TEF1p-SpCas9::KanR(in place of PID)-1xNLS-3xHA-1xNLS-Zif268-1xNLS-CYC1t
    

    Use

    CRISPR

    Tags

    3x HA, SV40 NLS, Zif268, and c-Myc-like NLS

    Expression

    Yeast

    Mutation

    Corrected homology relative to wild type SpCas9 (…

    Promoter

    TEF1

    Available Since

    April 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pRS313_ATP3G919C

    Plasmid

    #120255

    Purpose

    Expression of ATP3-7 in yeast

    Depositor

    Markus Ralser

    Article

    Vowinckel et al Nat Metab. 2021 Nov;3(11):1521-1535. doi

    Insert

    ATP3 (ATP3 Budding Yeast)
    

    Expression

    Yeast

    Mutation

    G919 changed to C; Alanine 307 changed to Proline

    Promoter

    ATP3

    Available Since

    April 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRS313_ATP3mut

    Plasmid

    #120256

    Purpose

    Expression of ATP3-67 in yeast

    Depositor

    Markus Ralser

    Article

    Vowinckel et al Nat Metab. 2021 Nov;3(11):1521-1535. doi

    Insert

    ATP3 (ATP3 Budding Yeast)
    

    Expression

    Yeast

    Mutation

    Isoleucine 304 changed to Asparagine; Alanine 307…

    Promoter

    ATP3

    Available Since

    April 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. CPH3568 (YCp LEU2 Rpb1 P1455_E1456InsGGGGGLEVLFQGP(H)10,N1501K; msLink1)

    Plasmid

    #91819

    Purpose

    Yeast expression of S. cerevisiae Rpb1 with an internal PreScission protease site and 10xHis tag for purification of the linker-CTD

    Depositor

    Chris Hill

    Article

    Sdano et al Elife. 2017 Aug 16;6. pii: 28723. doi: 1

    Insert

    RPO21 (RPO21 Budding Yeast)
    

    Tags

    10xHis

    Expression

    Yeast

    Mutation

    5xGly linker, PreScission Protease site (LEVLFQGP…

    Promoter

    Endogenous Rpb1 promoter

    Available Since

    March 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. CPH3569 (YCp LEU2 Rpb1 P1455_E1456InsGGGGGLEVLFQGP(H)10,K1487N,D1497K; msLink2)

    Plasmid

    #91820

    Purpose

    Yeast expression of S. cerevisiae Rpb1 with an internal PreScission protease site and 10xHis tag for purification of the linker-CTD

    Depositor

    Chris Hill

    Article

    Sdano et al Elife. 2017 Aug 16;6. pii: 28723. doi: 1

    Insert

    RPO21 (RPO21 Budding Yeast)
    

    Tags

    10xHis

    Expression

    Yeast

    Mutation

    5xGly linker, PreScission Protease site (LEVLFQGP…

    Promoter

    Endogenous Rpb1 promoter

    Available Since

    March 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pPICZ-aC-MSVU-BZ

    Plasmid

    #160426

    Purpose

    Expression of c-luciferase from sp. Maristella sp. SVU (Belize)

    Depositor

    Todd Oakley

    Article

    Hensley et al Mol Ecol. 2020 Oct 8. doi: 10.1111/mec.1

    Insert

    luciferase
    

    Use

    Luciferase

    Tags

    6x Histidine tag, Yeast alpha secretion factor, c…

    Expression

    Yeast

    Mutation

    See depositor comments below

    Promoter

    AOX

    Available Since

    Oct. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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