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pEntry_SARS-CoV-1-Spike

Plasmid

#168864

Purpose

Gateway-compatible Entry vector

Depositor

Pascal Falter-Braun

Article

Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

Insert

SARS-CoV-1-Spike (S )

Use

Gateway-compatible entry vector

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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PIK3C2B gRNA (BRDN0001149112)

Plasmid

#76713

Purpose

3rd generation lentiviral gRNA plasmid targeting human PIK3C2B

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PIK3C2B (PIK3C2B Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pCWXPGR-pTF-Wnt3a

Plasmid

#114287

Purpose

TET-inducible expression of Wnt3a

Depositor

Patrick Salmon

Article

Bocchi et al Nat Commun. 2017 Oct 27;8(1):1158. doi:

Insert

Wnt3a (Wnt3a Mouse)

Use

Lentiviral

Available Since

Nov. 16, 2018

Availability

Academic Institutions and Nonprofits only

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pJEP317-pAAV-U6SaCas9gRNA(SapI)-EFS-GFP- KASH-pA

Plasmid

#113694

Purpose

U6 driven SaCas9 gRNA expression cassette without a gRNA. Followed by an EFS driven GFP-KASH in a separate reading frame. SapI can be used to clone in gRNAs.

Depositor

Jonathan Ploski

Article

Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

Inserts

SaCas9 gRNA Cassete
GFP

Use

AAV and CRISPR

Tags

KASH

Available Since

Jan. 2, 2019

Availability

Academic Institutions and Nonprofits only

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mCh-KIF17*-strep

Plasmid

#120167

Purpose

Expresses a chimera of mCherry (fluorescent tag), a KIF17-based anterograde motor and streptavidin

Depositor

Juan Bonifacino

Article

Guardia et al PLoS Biol. 2019 May 17;17(5):e3000279. d

Insert

kinesin family member 17 (KIF17 Human)

Tags

Streptavidin and mCherry

Expression

Mammalian

Mutation

Truncated KIF17: 1-509 aa

Promoter

CMV

Available Since

July 30, 2019

Availability

Academic Institutions and Nonprofits only

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mCh-KIF13A*-strep

Plasmid

#120166

Purpose

Expresses a chimera of mCherry (fluorescent tag), a KIF13A-based anterograde motor and streptavidin

Depositor

Juan Bonifacino

Article

Guardia et al PLoS Biol. 2019 May 17;17(5):e3000279. d

Insert

kinesin family member 13A (KIF13A Human)

Tags

Streptavidin and mCherry

Expression

Mammalian

Mutation

Truncated KIF13A: 1-639 aa

Promoter

CMV

Available Since

July 30, 2019

Availability

Academic Institutions and Nonprofits only

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pUG-natNT2

Plasmid

#110922

Purpose

expression of nat gene conferring resistance to nourseothricin for gene deletion in yeast

Depositor

Jean-Marc Daran

Article

Juergens et al FEMS Yeast Res. 2018 May 1;18(3). pii: 4

Insert

nat

Expression

Bacterial and Yeast

Promoter

AgTEF1

Available Since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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PKD1 gRNA (BRDN0001149544)

Plasmid

#76841

Purpose

3rd generation lentiviral gRNA plasmid targeting human PKD1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PKD1 (PKD1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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TVBB C-term-mNeongreen

Plasmid

#169227

Purpose

Targeting vector backbone to support a knock-in of Linker-mNeongreen at the C-terminus of a target locus

Depositor

Hugo Snippert

Article

Bollen et al PLoS Biol. 2022 Jan 28;20(1):e3001527. d

Insert

Double SapI flanked mNeongreen

Use

Targeting vector backbone

Available Since

Jan. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pWPI-UPP1-3xFLAG

Plasmid

#201644

Purpose

Over-expression of 3xFLAG-tagged human UPP1

Depositor

Alexis Jourdain, Vamsi Mootha

Article

Skinner et al Nat Metab. 2023 May;5(5):765-776. doi: 1

Insert

UPP1 (UPP1 Human)

Use

Lentiviral

Tags

3xFLAG

Available Since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

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GFP-SBP-VAP-A*

Plasmid

#120175

Purpose

Expresses a chimera of GFP (fluorescent tag), SBP tag and the C-terminal of ER-resident protein VAP-A

Depositor

Juan Bonifacino

Article

Guardia et al PLoS Biol. 2019 May 17;17(5):e3000279. d

Insert

VAMP associated protein A (VAPA Human)

Tags

EGFP and SBP

Expression

Mammalian

Mutation

Truncated VAP-A: 118-249

Promoter

CMV

Available Since

July 30, 2019

Availability

Academic Institutions and Nonprofits only

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mCh-BICD2*-strep

Plasmid

#120168

Purpose

Expresses a chimera of mCherry (fluorescent tag), a BICD2-based adaptor and streptavidin

Depositor

Juan Bonifacino

Article

Guardia et al PLoS Biol. 2019 May 17;17(5):e3000279. d

Insert

BICD cargo adaptor 2 (Bicd2 Mouse)

Tags

Streptavidin and mCherry

Expression

Mammalian

Mutation

Truncated BICD2: 15-595 aa

Promoter

CMV

Available Since

July 30, 2019

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1_MeCP2(deltaNIC)

Plasmid

#110191

Purpose

Expression of mouse MeCP2 (deltaNIC) in mammalian cells

Depositor

Adrian Bird

Article

Tillotson et al Nature. 2017 Oct 19;550(7676):398-401. d

Insert

MeCP2_e2 delta NIC (mouse cDNA) - EGFP (Mecp2 Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

delta N and C termini. Intervening region replace…

Promoter

CMV

Available Since

May 16, 2018

Availability

Academic Institutions and Nonprofits only

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pJEP316-pAAV-U6SaCas9gRNA(SapI)-pA Empty Cassette

Plasmid

#113693

Purpose

U6 driven SaCas9 gRNA expression cassette without a gRNA. SapI can be used to clone in gRNAs.

Depositor

Jonathan Ploski

Article

Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

Insert

SaCas9 gRNA Cassete

Use

AAV and CRISPR

Available Since

Dec. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV_hSyn_NES-Kinprola_PKA-mEGFP_WRPE-SV40

Plasmid

#233366

Purpose

hSyn1 driven expression of the PKA activity recorder Kinprola_PKA fused to mEGFP for neuronal expression through AAV transduction

Depositor

Kai Johnsson

Article

Sun et al Nat Chem Biol. 2025 Jul 10. doi: 10.1038

Insert

NES-Kinprola_PKA-mEGFP

Use

AAV

Tags

NES and mEGFP

Promoter

hSyn1

Available Since

June 23, 2025

Availability

Academic Institutions and Nonprofits only

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pDONR223-VPS35

Plasmid

#21689

Depositor

Lynda Chin

Article

Scott et al Nature. 2009 Jun 25. 459(7250):1085-90.

Insert

Vacuolar Protein Sorting 35 homolog (VPS35 Human)

Use

Gateway entry clone

Mutation

last nucleotide of stop codon removed for c-termi…

Available Since

April 30, 2010

Availability

Academic Institutions and Nonprofits only
INCTbiosyn-pUB-HspINT2

Plasmid

#127512

Purpose

Plasmid encodes H. sapiens codon optimized Integrase 2.

Depositor

Martin Bonamino

Article

Gomide et al Commun Biol. 2020 May 22;3(1):255. doi:

Insert

Integrase 2 coding sequence codon optimized for H. sapiens expression.

Expression

Mammalian

Available Since

May 6, 2020

Availability

Academic Institutions and Nonprofits only

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INCTbiosyn-pUB-HspINT9

Plasmid

#127516

Purpose

Plasmid encodes H. sapiens codon optimized Integrase 9.

Depositor

Martin Bonamino

Article

Gomide et al Commun Biol. 2020 May 22;3(1):255. doi:

Insert

Integrase 9 coding sequence codon optimized for H. sapiens expression.

Expression

Mammalian

Available Since

May 6, 2020

Availability

Academic Institutions and Nonprofits only

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INCTbiosyn-pUB-HspINT13

Plasmid

#127517

Purpose

Plasmid encodes H. sapiens codon optimized Integrase 13.

Depositor

Martin Bonamino

Article

Gomide et al Commun Biol. 2020 May 22;3(1):255. doi:

Insert

Integrase 13 coding sequence codon optimized for H. sapiens expression.

Expression

Mammalian

Available Since

May 6, 2020

Availability

Academic Institutions and Nonprofits only

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pPB-3xnls-Tq-Ca-FLITS

Plasmid

#145030

Purpose

piggyBac vector for expressing a Turquoise calcium sensor (GECI) that reports with lifetime and intensity changes

Depositor

Dorus Gadella

Article

van der Linden et al Nat Commun. 2021 Dec 9;12(1):7159. doi:

Insert

3xnls-Tq-Ca-FLITS

Tags

3xnls-Tq-Ca-FLITS

Expression

Mammalian

Promoter

CMV

Available Since

June 23, 2021

Availability

Academic Institutions and Nonprofits only

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