We narrowed to 51,719 results for: Ide

1. pXPR_212

   Plasmid

   #133457

   Purpose

   U6 promoter expresses customizable Spyo-guide; EFS promoter expresses SaurCas9 and 2A site provides puromycin resistance

   Depositor

   John Doench

   Article

   DeWeirdt et al Nat Commun. 2020 Feb 6;11(1):752. doi: 1

   Insert

   control guide
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Oct. 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMT462

   Plasmid

   #139393

   Purpose

   XOR circuit (A000 and P000 design) with aTC and Bile Acid as inputs, AmpR, ErmR

   Depositor

   Christopher Voigt

   Article

   Taketani et al Nat Biotechnol. 2020 Aug;38(8):962-969.

   Insert

   XOR circuit (A000 and P000 design) with aTC and Bile Acid as inputs
   

   Use

   Synthetic Biology

   Promoter

   aTC and BA inducible promoters

   Available Since

   March 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pXPR_213

   Plasmid

   #133456

   Purpose

   H1 promoter expresses customizable Saur-guide; EF1a promoter expresses SpyoCas9 and 2A site provides blasticidin resistance

   Depositor

   John Doench

   Article

   DeWeirdt et al Nat Commun. 2020 Feb 6;11(1):752. doi: 1

   Insert

   Control guide
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Oct. 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. UNC50-myc

   Plasmid

   #104382

   Purpose

   Expresses UNC50-myc wild-type in mammalian cells

   Depositor

   Somshuvra Mukhopadhyay

   Article

   Selyunin et al J Cell Biol. 2017 Oct 2;216(10):3249-326

   Insert

   UNC50 (UNC50 Human)
   

   Tags

   myc

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJFRC7-UAS-NPRR_ANP

   Plasmid

   #133243

   Purpose

   Fluorescent reporter for neuropeptide release imaging

   Depositor

   David Anderson

   Article

   Ding et al Elife. 2019 Jun 26;8. pii: 46421. doi: 1

   Insert

   rat atrial natriuretic peptide-fused codon-optimized GCaMP6s
   

   Expression

   Insect

   Promoter

   hsp70

   Available Since

   Feb. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. Flag-nls-NgAgo-GK

   Plasmid

   #86435

   Purpose

   Expresses a Flag tag on the N-terminal

   Depositor

   Gaétan Burgio

   Article

   Khin et al PLoS One. 2017 Jun 13;12(6):e0178768. do

   Insert

   NgAgo
   

   Tags

   Flag tag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGL4-Gateway-SCP1

   Plasmid

   #71510

   Purpose

   humanSTARR-seq validation vector with Gateway cassette upstream of the firefly luciferase gene and the SCP1 core promoter

   Depositor

   Alexander Stark

   Article

   Arnold et al Science. 2013 Mar 1;339(6123):1074-7. do

   Type

   Empty backbone

   Use

   Humanstarr-seq validation vector

   Tags

   luciferase

   Expression

   Mammalian

   Available Since

   Jan. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

8. pRDA_186

   Plasmid

   #133458

   Purpose

   U6 promoter expresses customizable Spyo-guide; PGK promoter expresses blasticidin resistance and 2A site provides EGFP

   Depositor

   John Doench

   Article

   DeWeirdt et al Nat Commun. 2020 Feb 6;11(1):752. doi: 1

   Insert

   control guide
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Oct. 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET16b-Antp

   Plasmid

   #86866

   Purpose

   Bacterial expression of fusion protein with cell penetrating peptide, antennapedia fragment, at C-terminus.

   Depositor

   Michael Chin

   Article

   Chien et al Genesis. 2014 Jul;52(7):695-701. doi: 10

   Insert

   Linker-Antp
   

   Tags

   Antp

   Expression

   Bacterial

   Available Since

   April 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBS-beta1,4 GT-1

    Plasmid

    #11872

    Depositor

    Michiko Fukuda

    Article

    Masri et al Biochem Biophys Res Commun. 1988 Dec 15.

    Insert

    beta 1,4 galactosyltransferase (B4GALT1 Human)
    

    Expression

    Bacterial

    Available Since

    May 12, 2006

    Availability

    Academic Institutions and Nonprofits only

11. pGEX-2T SHP1 CS

    Plasmid

    #8595

    Depositor

    Ben Neel

    Article

    Timms et al Mol Cell Biol 1998 Jul;18(7):3838-50.

    Insert

    SHP1 (PTPN6 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    C453S (catalytically inactive)

    Available Since

    June 21, 2005

    Availability

    Academic Institutions and Nonprofits only

12. pET14_GFP5_Encap

    Plasmid

    #86408

    Purpose

    Expresses sfGFP tagged with a 5 amino acid CLP and encapsulin as an operon

    Depositor

    David Savage

    Article

    Cassidy-Amstutz et al Biochemistry. 2016 Jun 21;55(24):3461-8.

    Inserts

    sfGFP with a 5aa CLP tag
    Encapsulin
    

    Tags

    6x Histidine

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. HsCERK

    Plasmid

    #118600

    Purpose

    Expresses ceramide kinase, which catalyzes the phosphorylation of ceramide to form ceramide 1-phosphate

    Depositor

    Kevin Lynch

    Article

    Kharel et al PLoS One. 2018 Apr 19;13(4):e0192179. do

    Insert

    Homo sapiens ceramide kinase
    

    Tags

    FLAG

    Expression

    Yeast

    Promoter

    GAL1, 10

    Available Since

    Feb. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEGFP-(RTRG)6

    Plasmid

    #69849

    Purpose

    The RTRG repeat act as a nuclear/cytoplasmic partition signal in the expressed fusion protein providing increased nuclear to cytoplasmic partition ratio when increasing the number of repeats

    Depositor

    Daniel Christophe

    Article

    Christophe-Hobertus et al Protein Pept Lett. 2008;15(4):397-401.

    Insert

    6 RTRG repeats
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pJZ001

    Plasmid

    #120230

    Purpose

    Contains intB13 integrase casette for knock-in to pCAP-BAC backbone

    Depositor

    Bradley Moore

    Article

    Bauman et al Cell Chem Biol. 2019 Feb 15. pii: S2451-

    Insert

    intB13 integrase casette
    

    Expression

    Bacterial

    Mutation

    bla-g717a (amp resistance gene)

    Available Since

    March 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pPIC-mouseKir3.2-mC-S

    Plasmid

    #140745

    Purpose

    Expression of mouse Kir3.2 fused to mCherry and Streptag II

    Depositor

    Arthur Laganowsky

    Article

    Qiao et al Biochemistry. 2020 May 6. doi: 10.1021/a

    Insert

    Kir3.2 (Kcnj6 Mouse)
    

    Tags

    mCherry with Streptag II

    Expression

    Yeast

    Promoter

    AOX1

    Available Since

    May 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pmFgf7

    Plasmid

    #31237

    Depositor

    Cliff Ragsdale

    Article

    Assimacopoulos et al J Neurosci. 2003 Jul 23. 23(16):6399-403

    Insert

    Fgf7 (Fgf7 Mouse)
    

    Use

    Cdna for in situ hybridization

    Available Since

    Sept. 15, 2011

    Availability

    Academic Institutions and Nonprofits only

18. pGL2084

    Plasmid

    #137726

    Purpose

    Gateway vector for inducible stem loop RNAi in T.b. brucei 2T1

    Depositor

    Jeremy Mottram

    Article

    Jones et al PLoS Pathog. 2014 Jan;10(1):e1003886. do

    Type

    Empty backbone

    Use

    RNAi; Trypanosoma brucei tet inducible stem loop

    Available Since

    June 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET28a-FFSARG-ddFLN4-HIS-ybbr

    Plasmid

    #101240

    Purpose

    minimal SdrG binding peptide FFSARG with ddFLN4 fingerprint domain with C-terminal ybbr tag for covalent immobilization - resulting in high forces

    Depositor

    Hermann Gaub

    Article

    Milles et al Science. 2018 Mar 30;359(6383):1527-1533

    Insert

    FFSARG peptide, ddFLN4 fingerprint
    

    Tags

    HIS and ybbr

    Expression

    Bacterial

    Mutation

    C18S in ddFLN4

    Promoter

    T7

    Available Since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pJB172

    Plasmid

    #86992

    Purpose

    CRISPR/Cas9 in fission yeast using fluoride selection and targetting pil1

    Depositor

    Julien Berro

    Article

    Fernandez et al Yeast. 2016 Oct;33(10):549-557. doi: 10.

    Insert

    gRNA targeting pil1
    

    Expression

    Yeast

    Available Since

    Feb. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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