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4A3.PBAD-repL(AT2).J23115-gfp

Plasmid

#197873

Purpose

Arabinose induced expression of repL(AT2) gene, which triggers DNA replication in trans, weak level of constitutive GFP expression

Depositor

Baojun Wang

Article

Huan et al Front Microbiol. 2023 Feb 9;14:1095671.

Insert

part kilA/repL(AT2)

Expression

Bacterial

Promoter

PBAD

Available Since

June 14, 2023

Availability

Academic Institutions and Nonprofits only

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pAT9675-CBE

Plasmid

#163007

Purpose

plasmid expressing CBE

Depositor

Ervin Welker

Article

Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

Insert

CBE

Use

CRISPR

Tags

3xFLAG and SV40 NLS

Expression

Mammalian

Promoter

EF1-a

Available Since

Sept. 21, 2021

Availability

Academic Institutions and Nonprofits only

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p4A8_S7T_BC

Plasmid

#217832

Purpose

4A8 variant in barcoded, yeast surface display plasmid

Depositor

Timothy Whitehead

Article

Petersen et al Nat Commun. 2024 May 10;15(1):3974. doi:

Insert

4A8 variant Fab

Expression

Yeast

Mutation

VL: S7T

Available Since

April 24, 2024

Availability

Academic Institutions and Nonprofits only

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pXTK066 [Con-Pro_nptII-Broad_PartType8]

Plasmid

#229303

Purpose

XanthoMoClo Golden Gate Part Plasmid for cloning, contains Constitutive-Promoter_nptII-Broad_PartType8

Depositor

Michael Springer

Article

Soltysiak et al ACS Synth Biol. 2025 Apr 18;14(4):1173-1

Insert

nptII Promoter (Broad)

Expression

Bacterial

Available Since

March 14, 2025

Availability

Academic Institutions and Nonprofits only

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LC301: pMVP/PB/Zeo-DEST

Plasmid

#121877

Purpose

pMVP destination vector, empty PiggyBac transposon vector backbone w/ Zeo selection cassette

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Type

Empty backbone

Use

Piggybac transposon, pmvp gateway destination vec…

Expression

Mammalian

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pAT9748-dCBE

Plasmid

#163008

Purpose

plasmid expressing CBE with dCas9

Depositor

Ervin Welker

Article

Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

Insert

dCBE

Use

CRISPR

Tags

3xFLAG and SV40 NLS

Expression

Mammalian

Promoter

EF1-a

Available Since

Sept. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pENTR4 TNRC6B

Plasmid

#20022

Depositor

Thomas Tuschl

Article

Landthaler et al RNA. 2008 Dec;14(12):2580-96. doi: 10.12

Insert

Trinucleotide repeat containing 6B (TNRC6B Human)

Use

Entry vector

Available Since

Jan. 12, 2009

Availability

Academic Institutions and Nonprofits only

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OVA-miSFIT-17-T

Plasmid

#124677

Purpose

Tuning expression of cytosolic Ovalbumin coupled to EGFP

Depositor

Tudor Fulga

Article

Michaels et al Nat Commun. 2019 Feb 18;10(1):818. doi:

Insert

Cyto-Ovalbumin

Expression

Mammalian

Mutation

Truncated OVA for cytoplasmic expression

Promoter

minimal CMV

Available Since

May 8, 2019

Availability

Academic Institutions and Nonprofits only

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pSUMO-YHRC

Plasmid

#54336

Purpose

Expression vector (pT7) for untagged expression as well as fusions to His6-SUMO or His6 tags. Designed for single or multiple fragment cloning by yeast homologous recombination.

Depositor

Claes Andréasson

Article

Holmberg et al Protein Expr Purif. 2014 Jun;98:38-45. d

Type

Empty backbone

Tags

6His-SUMO (yeast gene: SMT3)

Expression

Bacterial

Promoter

T7-LacO

Available Since

Aug. 1, 2014

Availability

Academic Institutions and Nonprofits only

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MPRA Empty Vector

Plasmid

#204865

Purpose

Vector used for high-throughput cloning and subsequent transfection of MPRA libraries into mammalian cells.

Depositor

Philipp Maass

Article

Oliveros et al Cell Genom. 2023 May 24;3(7):100330. doi

Type

Empty backbone

Expression

Bacterial and Mammalian

Available Since

Sept. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pCMU-4

Plasmid

#172353

Purpose

CRISPR gene editing in Streptomyces, codon-optimized Cas9 behind theophylline riboswitch and D4 promoter, with custom gRNA

Depositor

Eriko Takano

Article

Ye et al J Ind Microbiol Biotechnol. 2020 May;47(

Insert

D4-TheoR-cas9

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Promoter

D4

Available Since

Dec. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pAGM55297

Plasmid

#153213

Purpose

Level 2 cloning vector for a single guide RNA, already contains Cas9, Kanamycin selection cassette for plant transformation and FAST marker

Depositor

Sylvestre Marillonnet

Article

Grutzner et al Plant Commun. 2020 Nov 23;2(2):100135. d

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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HB501: pMVP (L1-L4) RIP

Plasmid

#121688

Purpose

pMVP L1-L4 entry plasmid, contains rat Ins1 promoter (RIP) for 3-component MultiSite Gateway Pro assembly

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

Rat Ins1 promoter (RIP; β-cell specific)

Use

Synthetic Biology; Pmvp gateway entry plasmid

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pKB01-PssbB-mKate2

Plasmid

#69105

Purpose

Expresses mKate2 (codon adapted for Streptococcus pneumoniae); under control of CSP inducible promoter PssbB. Plasmid integrates in S. pneumoniae bgaA locus by double cross-over.

Depositor

Jan-Willem Veening

Article

Beilharz et al Appl Environ Microbiol. 2015 Aug 7. pii:

Insert

mKate2

Use

Synthetic Biology

Expression

Bacterial

Available Since

April 27, 2016

Availability

Academic Institutions and Nonprofits only

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pAGM65905

Plasmid

#153217

Purpose

Level 2 cloning vector for one or several guide RNAs, already contains Cas9 and a kanamycin selection cassette for plant transformation

Depositor

Sylvestre Marillonnet

Article

Grutzner et al Plant Commun. 2020 Nov 23;2(2):100135. d

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pHS1184

Plasmid

#205965

Purpose

NZ_CP025815 His14-TwinStrep-bdSUMO tagged DinG HNH protein (E. coli codon optimized) in pET45b(+)

Depositor

Feng Zhang

Article

Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

Insert

DinG HNH

Tags

14xHis-TwinStrep-bdSUMO

Expression

Bacterial

Promoter

T7

Available Since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

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AlkB-D135T

Plasmid

#160433

Purpose

A plasmid for expression in E. coli to produce His-tag fusion protein AlkB with Asp at position 135 mutated to Thr

Depositor

Tao Pan

Article

Wang et al Nucleic Acids Res. 2021 Mar 18;49(5):e30

Insert

E. coli AlkB

Tags

His-tag

Expression

Bacterial

Mutation

Changed Aspartic acid 135 to Threonine; deleted t…

Promoter

T7

Available Since

Dec. 8, 2020

Availability

Academic Institutions and Nonprofits only

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pRS306-pHIS3-2xGFP-tURA3

Plasmid

#116936

Purpose

Yeast integrative vector for the expression of 2xGFP

Depositor

Liam Holt

Article

Delarue et al Cell. 2018 Jul 12;174(2):338-349.e20. do

Insert

2xGFP

Expression

Yeast

Promoter

HIS3

Available Since

Jan. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pRepair-MPP8_mAID-P2A-BFP

Plasmid

#175036

Purpose

Homology Directed Repair construct for C-terminal tagging of mMPP8 with mAID using CRISPR/Cas9

Depositor

Kristian Helin

Article

Muller et al Nat Commun. 2021 May 24;12(1):3034. doi:

Insert

mAID-P2A-BFP

Use

Hdr donor template

Available Since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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LD101: pMVP (L3-L2) eCFP + WPRE

Plasmid

#121775

Purpose

pMVP L3-L2 entry plasmid, contains eCFP + WPRE for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term eCFP fusion to gene of interest in lentivirus vectors.

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

eCFP + WPRE

Use

Synthetic Biology; Pmvp gateway entry plasmid

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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