We narrowed to 9,760 results for: Ada;

1. pLKO.1-shGBP1.2.mKO2

   Plasmid

   #85210

   Purpose

   TRCN0000116120 (Target TGAGACGACGAAAGGCATGTA) for silencing GBP1 gene and express monomeric Kusabira-Orange2.

   Depositor

   Sandra Martha Gomes Dias

   Article

   Quintero et al BMC Cancer. 2017 Nov 7;17(1):727. doi: 1

   Insert

   GBP1 (guanylate binding protein 1) (GBP1 Human)
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   RNA polymerase III promoter for human U6 snRNA fo…

   Available since

   Oct. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. GST-beta2(616-951)∆CBM

   Plasmid

   #100745

   Purpose

   Bacterial expression of GST-tagged beta2 subunit of AP2 complex (hinge and ear) long splice form, mutant

   Depositor

   Stephen Royle

   Article

   Wood et al J Cell Biol. 2017 Sep 27. pii: jcb.20170

   Insert

   beta2 adaptin (AP2B1 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Long isoform with deletion of clathrin binding mo…

   Promoter

   Available since

   Sept. 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLuc-OptoJNKi

   Plasmid

   #89744

   Purpose

   Expression of light-regulated JNK inhibitor, firefly luciferase-fused, wild-type photosensor, inhibitor JIP11

   Depositor

   Michael Courtney

   Article

   Melero-Fernandez de Mera et al Nat Commun. 2017 May 12;8:15017. doi: 10

   Insert

   OptoJNKi
   

   Use

   Luciferase

   Tags

   Firefly luciferase

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3/ TO myc-cCE-bio (cCE-bio)

   Plasmid

   #82473

   Purpose

   Expresses cytoplasmic restricted form of N-terminal myc tagged and C-terminal biotin tagged mRNA capping enzyme, inactive form

   Depositor

   Daniel Schoenberg

   Article

   Mukherjee et al PLoS Biol. 2014 Aug 19;12(8):e1001933. d

   Insert

   Myc-NES-mCE (Wt, NLS deletion)-TEV-Bio (Rngtt Synthetic, Mouse)
   

   Use

   Tags

   TEV-Biotin and myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBullet-cyt-n

   Plasmid

   #53065

   Purpose

   destination vector with cytosol marker (ECFP) for tagged fluorescent protein (N-ter-EYFP) of plant gene

   Depositor

   Joshua Heazlewood

   Article

   Lao et al Plant J. 2014 Jun 6. doi: 10.1111/tpj.12

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   ECFP and EYFP

   Expression

   Mutation

   Promoter

   Available since

   July 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBullet-er-n

   Plasmid

   #53067

   Purpose

   destination vector with WAK2 (29 aa)-ECFP- ER marker (HDEL) for tagged fluorescent protein (N-ter-EYFP) of plant gene

   Depositor

   Joshua Heazlewood

   Article

   Lao et al Plant J. 2014 Jun 6. doi: 10.1111/tpj.12

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   ECFP and EYFP

   Expression

   Mutation

   Promoter

   Available since

   July 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBullet-tgn-n

   Plasmid

   #53071

   Purpose

   destination vector with ECFP-trans golgi network marker (VTI12) for tagged fluorescent protein (N-ter-EYFP) of plant gene

   Depositor

   Joshua Heazlewood

   Article

   Lao et al Plant J. 2014 Jun 6. doi: 10.1111/tpj.12

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEGFP-C1-AR Q48

   Plasmid

   #86428

   Purpose

   Fluorescent human androgen receptor (fused to EGFP) with expanded polyglutamine region

   Depositor

   Michael Mancini

   Article

   Stenoien et al Hum Mol Genet. 1999 May . 8(5):731-41.

   Insert

   Androgen receptor (AR) (AR Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Poly-glutamine expansion, G130D

   Promoter

   CMV

   Available since

   April 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. HA-HA-AR-DBD

   Plasmid

   #171238

   Purpose

   Mammalian expression of 2xHA-tagged human androgen receptor trancate: AR DNA-bindind domain (DBD)

   Depositor

   Steven Balk

   Article

   Liu et al Oncotarget. 2016 Jan 12;7(2):1754-64. do

   Insert

   2xHA-tagged human androgen receptor trancate:DNA-binding domain (AR Human)
   

   Use

   Tags

   HA

   Expression

   Bacterial and Mammalian

   Mutation

   AR truncate

   Promoter

   CMV

   Available since

   June 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. HA-HA-AR-NTD

    Plasmid

    #171235

    Purpose

    Mammalian expression of 2xHA-tagged human androgen receptor trancate: AR N-terminal-domain (NTD)

    Depositor

    Steven Balk

    Article

    Liu et al Oncotarget. 2016 Jan 12;7(2):1754-64. do

    Insert

    2xHA-tagged human androgen receptor trancate: N-terminal-domain (AR Human)
    

    Use

    Tags

    HA

    Expression

    Bacterial and Mammalian

    Mutation

    AR truncate

    Promoter

    CMV

    Available since

    June 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. HA-AR-LBD

    Plasmid

    #171239

    Purpose

    Mammalian expression of 1xHA-tagged human androgen receptor trancate: AR Ligand-bindind domain (LBD)

    Depositor

    Steven Balk

    Article

    Liu et al Oncotarget. 2016 Jan 12;7(2):1754-64. do

    Insert

    1xHA-tagged human androgen receptor trancate:Ligand-binding domain (AR Human)
    

    Use

    Tags

    HA

    Expression

    Bacterial and Mammalian

    Mutation

    AR truncate

    Promoter

    CMV

    Available since

    June 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pESF140_HisSpy_DDB1∆B

    Plasmid

    #124213

    Purpose

    Insect cell expression vector for His6, Spy tagged hsDDB1∆B

    Depositor

    Eric Fischer

    Article

    Nowak et al Nat Chem Biol. 2018 Jul;14(7):706-714. d

    Insert

    DNA damage-binding protein 1 (DDB1 Human)
    

    Use

    Tags

    His6-Spy

    Expression

    Insect

    Mutation

    internal deletion of BPB domain to aid crystalliz…

    Promoter

    Available since

    April 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. CaMKII-somBiPOLES-mCerulean

    Plasmid

    #154948

    Purpose

    Optogenetic tool for blue-light inhibition and red-light excitation of neurons

    Depositor

    Simon Wiegert

    Article

    Vierock et al Nat Commun. 2021 Jul 26;12(1):4527. doi:

    Has Service

    AAV Retrograde, AAV5, and AAV9

    Insert

    somBiPOLES
    

    Use

    AAV

    Tags

    soma-targeting motif from Kv2.1 channel

    Expression

    Mammalian

    Mutation

    Promoter

    CaMKIIa(0.4)

    Available since

    Aug. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCMV-PEmax

    Plasmid

    #174820

    Purpose

    Mammalian expression of SpCas9 PEmax prime editor

    Depositor

    David Liu

    Article

    Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

    Insert

    PEmax
    

    Use

    Tags

    SV40 bpNLS and c-Myc NLS

    Expression

    Mammalian

    Mutation

    Detailed in manuscript

    Promoter

    CMV

    Available since

    Oct. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pT7-PEmax for IVT

    Plasmid

    #178113

    Purpose

    Template for in vitro transcription of PEmax

    Depositor

    David Liu

    Article

    Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

    Insert

    PEmax
    

    Use

    Template for in vitro transcription

    Tags

    SV40 bpNLS and c-Myc NLS

    Expression

    Mutation

    Detailed in manuscript

    Promoter

    T7 (inactivated)

    Available since

    Nov. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV_hsyn_NES-his-CaMPARI2-WPRE-SV40

    Plasmid

    #101060

    Purpose

    AAV vector expressing CaMPARI2 (Kd = 200nM), a photoconvertible fluorescent protein-based calcium integrator, in neurons

    Depositor

    Eric Schreiter

    Article

    Moeyaert et al Nat Commun. 2018 Oct 25;9(1):4440. doi:

    Has Service

    AAV1

    Insert

    CaMPARI2
    

    Use

    AAV

    Tags

    FLAG-HA-myc and NES_his

    Expression

    Mutation

    Promoter

    hsyn (synapsin-1)

    Available since

    Oct. 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLentiCrisprV2 sgRNA hCRY1 c-term

    Plasmid

    #179453

    Purpose

    Lentiviral Crispr/Cas9 plasmid targeting hCRY1 at C-terminus to generate C-terminal knock-in

    Depositor

    Achim Kramer

    Article

    Gabriel et al Nat Commun. 2021 Jun 18;12(1):3796. doi:

    Insert

    sgRNA targeting human CRY1
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    hU6

    Available since

    March 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCRIS-PITChv2-Puro-dTAG (BRD4)

    Plasmid

    #91793

    Purpose

    PITCh dTAG donor vector for Puro-P2A-2xHA-FKBP_F36V knock-in into the N-terminus of the human BRD4 locus.

    Depositor

    Behnam Nabet, James Bradner

    Article

    Nabet et al Nat Chem Biol. 2018 May;14(5):431-441. d

    Insert

    Puro-P2A-2xHA-FKBP_F36V
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Phenylalanine 36 to valine in FKBP12

    Promoter

    Promotorless

    Available since

    March 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEGFP-C2-MVP

    Plasmid

    #204543

    Purpose

    Expresses EGFP-tagged MVP in mammalian cells

    Depositor

    Masaaki Komatsu

    Article

    Kurusu et al Dev Cell. 2023 May 9:S1534-5807(23)00191

    Insert

    MVP (MVP Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    None

    Promoter

    Available since

    Aug. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCRIS-PITChv2-dTAG-Puro (BRD4)

    Plasmid

    #91796

    Purpose

    PITCh dTAG donor vector for FKBP_F36V-2xHA-P2A-Puro knock-in into the C-terminus of the human BRD4 locus.

    Depositor

    Behnam Nabet, James Bradner

    Article

    Nabet et al Nat Chem Biol. 2018 May;14(5):431-441. d

    Insert

    FKBP_F36V-2xHA-P2A-Puro
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Phenylalanine 36 to valine in FKBP12

    Promoter

    Promotorless

    Available since

    March 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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